Many plants contain ribosome inactivating proteins (RIPs) with N-glycosidase activity, which depurinate large ribosomal RNA and arrest protein synthesis. RIPs so far tested inhibit replication of mRNA as well as DNA v...Many plants contain ribosome inactivating proteins (RIPs) with N-glycosidase activity, which depurinate large ribosomal RNA and arrest protein synthesis. RIPs so far tested inhibit replication of mRNA as well as DNA viruses and these proteins, isolated from plants, are found to be effective against a broad range of viruses such as human immunodeficiency virus (HIV), hepatitis B virus (HBV) and herpes simplex virus (HSV). Most of the research work related to RIPs has been focused on antiviral activity against HIV; however, the exact mechanism of antiviral activity is still not clear. The mechanism of antiviral activity was thought to follow inactivation of the host cell ribosome, leading to inhibition of viral protein translation and host cell death. Enzymatic activity of RIPs is not limited to depurination of the large rRNA, in addition they can depurinate viral DNA as well as RNA. Recently, Phase I/II clinical trials have demonstrated the potential use of RIPs for treating patients with HIV disease. The aim of this review is to focus on various RIPs from plants associated with anti-HIV activity.展开更多
Ribosome-inactivating proteins(RIPs) belong to a family of enzymes that attack eukaryotic ribosomes and potently inhibit cellular protein synthesis.RIPs possess several biomedical properties,including anti-viral and a...Ribosome-inactivating proteins(RIPs) belong to a family of enzymes that attack eukaryotic ribosomes and potently inhibit cellular protein synthesis.RIPs possess several biomedical properties,including anti-viral and anti-tumor activities.Multiple RIPs are known to inhibit tumor cell proliferation through inducing apoptosis in a variety of cancers,such as breast cancer,leukemia/lymphoma,and hepatoma.This review focuses on the anti-tumor activities of RIPs and their apoptotic effects through three closely related pathways:mitochondrial,death receptor,and endoplasmic reticulum pathways.展开更多
A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 col...A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of-29 kD. It is a rRNA Nglycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a ICs0 of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M21 with a IC50 of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent.展开更多
Genomic DNA for Jatropha curcas ribosome inactivating protein (JRIP) was cloned from total DNA of its leaves by polymerase chain reaction (PCR). The no intron character was confirmed. The plant expression vector p...Genomic DNA for Jatropha curcas ribosome inactivating protein (JRIP) was cloned from total DNA of its leaves by polymerase chain reaction (PCR). The no intron character was confirmed. The plant expression vector pBI121-JRIP was constructed by inserting the JRIP gene into pBI121 plasmid. The recombinant Agrobacterium EHA105 strain harboring pBI121-JRIP was constructed by conducting pBI121-JRIP to strain EHA 105. PCR and Southern blotting were carried out, and the results proved that the JRIP gene was integrated into tobacco genome. It might provide a new material for disease resistance tobacco species breeding.展开更多
Cucurmosin 2, a type 1 ribosome-inactivating protein (RIP) isolated from sarcocarp of Cucurbita moschata, has been crystallized by the vapor-diffusion method using PEG6000 as the precipitant. The crystals belong to ...Cucurmosin 2, a type 1 ribosome-inactivating protein (RIP) isolated from sarcocarp of Cucurbita moschata, has been crystallized by the vapor-diffusion method using PEG6000 as the precipitant. The crystals belong to the orthorhombic space group P212121, with unit cell parameters a = 55.853, b = 65.507, c = 91.754 А, and have one molecule per asymmetric unit. X-ray data have been collected to 1.8А, using a synchrotron source.展开更多
Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins (RIPs). It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant...Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins (RIPs). It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813, c = 83.571 A, α = 891068,β = 80.009 and y = 72.143°, and has two molecules per asymmetric unit. X-ray data have been collected to be 1.4 A using a synchrotron source.展开更多
The interaction between ribosome-inactivating proteins (RIPs) and supercoiled DNA was observed with an atomic force microscope (AFM). It was found that RIPs can bind to both supercoiled DNA and the unwound double stra...The interaction between ribosome-inactivating proteins (RIPs) and supercoiled DNA was observed with an atomic force microscope (AFM). It was found that RIPs can bind to both supercoiled DNA and the unwound double stranded loop region in supercoiled DNA. The RIPs hound to the supercoils can induce the conformational change of supercoiled DNA. Furthermore, the supercoiled DNA was relaxed and cleaved into nick or linear form by RIPs. It indicated that RIP seemed to be a supercoil-dependent DNA binding protein and exhibited the activity of su-percoil-dependent DNA endonuclease.展开更多
核糖体失活蛋白(ribosome-inactivating proteins,RIPs)是一类能够脱去真核细胞28S r RNA内SRL区域的A4342,从而破坏延伸因子与核糖体的结合,将蛋白质的生物合成抑制在延伸阶段的蛋白质家族。RIPs有Ⅰ、Ⅱ、Ⅲ型,苦瓜中已发现的α-苦瓜...核糖体失活蛋白(ribosome-inactivating proteins,RIPs)是一类能够脱去真核细胞28S r RNA内SRL区域的A4342,从而破坏延伸因子与核糖体的结合,将蛋白质的生物合成抑制在延伸阶段的蛋白质家族。RIPs有Ⅰ、Ⅱ、Ⅲ型,苦瓜中已发现的α-苦瓜素、β-苦瓜素、γ-苦瓜素、δ-苦瓜素、ε-苦瓜素、MAP30等,均属于Ⅰ型RIPs。这些RIPs具有抗病毒、抗菌、抗虫害、抑制肿瘤细胞生长等生物学活性,受到了人们的广泛关注。本文从RIPs的分类、生物学活性、功能与应用等方面,对苦瓜中的RIPs进行了综述。展开更多
基金Indo-Swiss Joint research Program (ISJRP)#17/2011
文摘Many plants contain ribosome inactivating proteins (RIPs) with N-glycosidase activity, which depurinate large ribosomal RNA and arrest protein synthesis. RIPs so far tested inhibit replication of mRNA as well as DNA viruses and these proteins, isolated from plants, are found to be effective against a broad range of viruses such as human immunodeficiency virus (HIV), hepatitis B virus (HBV) and herpes simplex virus (HSV). Most of the research work related to RIPs has been focused on antiviral activity against HIV; however, the exact mechanism of antiviral activity is still not clear. The mechanism of antiviral activity was thought to follow inactivation of the host cell ribosome, leading to inhibition of viral protein translation and host cell death. Enzymatic activity of RIPs is not limited to depurination of the large rRNA, in addition they can depurinate viral DNA as well as RNA. Recently, Phase I/II clinical trials have demonstrated the potential use of RIPs for treating patients with HIV disease. The aim of this review is to focus on various RIPs from plants associated with anti-HIV activity.
基金the Basic Research Program of Shenzhen(JCYJ20120613113228732)the University Innovation Program of Guangdong Province (201410590040)
文摘Ribosome-inactivating proteins(RIPs) belong to a family of enzymes that attack eukaryotic ribosomes and potently inhibit cellular protein synthesis.RIPs possess several biomedical properties,including anti-viral and anti-tumor activities.Multiple RIPs are known to inhibit tumor cell proliferation through inducing apoptosis in a variety of cancers,such as breast cancer,leukemia/lymphoma,and hepatoma.This review focuses on the anti-tumor activities of RIPs and their apoptotic effects through three closely related pathways:mitochondrial,death receptor,and endoplasmic reticulum pathways.
文摘A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of-29 kD. It is a rRNA Nglycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a ICs0 of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M21 with a IC50 of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent.
基金Project supported by Tenth Five Years Key Program Foundation of the State Science and Technology Commission of China (GrantNo .2002BA901A15)
文摘Genomic DNA for Jatropha curcas ribosome inactivating protein (JRIP) was cloned from total DNA of its leaves by polymerase chain reaction (PCR). The no intron character was confirmed. The plant expression vector pBI121-JRIP was constructed by inserting the JRIP gene into pBI121 plasmid. The recombinant Agrobacterium EHA105 strain harboring pBI121-JRIP was constructed by conducting pBI121-JRIP to strain EHA 105. PCR and Southern blotting were carried out, and the results proved that the JRIP gene was integrated into tobacco genome. It might provide a new material for disease resistance tobacco species breeding.
基金supported by the National Natural Science Foundation of China (No. 39970872, 30772587)the Natural Science Foundation of Fujian Province (C97052,C0510012)+1 种基金Special Fund of Fujian Development and Reform Commission (2006-34)supported by the National Science Foundation of USA
文摘Cucurmosin 2, a type 1 ribosome-inactivating protein (RIP) isolated from sarcocarp of Cucurbita moschata, has been crystallized by the vapor-diffusion method using PEG6000 as the precipitant. The crystals belong to the orthorhombic space group P212121, with unit cell parameters a = 55.853, b = 65.507, c = 91.754 А, and have one molecule per asymmetric unit. X-ray data have been collected to 1.8А, using a synchrotron source.
基金This work was supported by the National Natural Science Foundation of China (No. 39970872) and Natural Science Foundation of Fujian Province (C97052)
文摘Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins (RIPs). It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813, c = 83.571 A, α = 891068,β = 80.009 and y = 72.143°, and has two molecules per asymmetric unit. X-ray data have been collected to be 1.4 A using a synchrotron source.
基金Project supported by the National Natural Science Foundation of China, Chinese Academy of Sciences and Shanghai Center of Life Sciences.
文摘The interaction between ribosome-inactivating proteins (RIPs) and supercoiled DNA was observed with an atomic force microscope (AFM). It was found that RIPs can bind to both supercoiled DNA and the unwound double stranded loop region in supercoiled DNA. The RIPs hound to the supercoils can induce the conformational change of supercoiled DNA. Furthermore, the supercoiled DNA was relaxed and cleaved into nick or linear form by RIPs. It indicated that RIP seemed to be a supercoil-dependent DNA binding protein and exhibited the activity of su-percoil-dependent DNA endonuclease.
文摘核糖体失活蛋白(ribosome-inactivating proteins,RIPs)是一类能够脱去真核细胞28S r RNA内SRL区域的A4342,从而破坏延伸因子与核糖体的结合,将蛋白质的生物合成抑制在延伸阶段的蛋白质家族。RIPs有Ⅰ、Ⅱ、Ⅲ型,苦瓜中已发现的α-苦瓜素、β-苦瓜素、γ-苦瓜素、δ-苦瓜素、ε-苦瓜素、MAP30等,均属于Ⅰ型RIPs。这些RIPs具有抗病毒、抗菌、抗虫害、抑制肿瘤细胞生长等生物学活性,受到了人们的广泛关注。本文从RIPs的分类、生物学活性、功能与应用等方面,对苦瓜中的RIPs进行了综述。