Anther culture is widely used in rice improve-ment and genome research.It is useful forgene transformation to stabilize foreign gene(s)and estimate the integrated copy number.We used two transgenic plants JB-3 andJB-4...Anther culture is widely used in rice improve-ment and genome research.It is useful forgene transformation to stabilize foreign gene(s)and estimate the integrated copy number.We used two transgenic plants JB-3 andJB-4 as donors for anther culture.Their originwas a japonica cultivar Jingyin 119,whose im-mature embryos were transformed by particlebombardment with plasmid pCB1(a cecropin B展开更多
In this paper,we describe briefly an efficientculture procedure for micro-propagation of riceregeneration plants.The procedure consists ofthe following steps: 1.Preparation of materials Calli wereinduced from mature ...In this paper,we describe briefly an efficientculture procedure for micro-propagation of riceregeneration plants.The procedure consists ofthe following steps: 1.Preparation of materials Calli wereinduced from mature seeds of an indica rice lineG67 by culturing the husked and disinfectedseeds on agar medium,consisting of Nbasalelements,3％ sucrose,1000mg/L proline,and 32mg/L 2,4-D.After one month＇s in- duction culture and 3 wk subculture,compactand nodular calli were picked out and trans- ferred into liquid medium for suspension cul-tures.The liquid medium contained the same展开更多
文摘Anther culture is widely used in rice improve-ment and genome research.It is useful forgene transformation to stabilize foreign gene(s)and estimate the integrated copy number.We used two transgenic plants JB-3 andJB-4 as donors for anther culture.Their originwas a japonica cultivar Jingyin 119,whose im-mature embryos were transformed by particlebombardment with plasmid pCB1(a cecropin B
文摘In this paper,we describe briefly an efficientculture procedure for micro-propagation of riceregeneration plants.The procedure consists ofthe following steps: 1.Preparation of materials Calli wereinduced from mature seeds of an indica rice lineG67 by culturing the husked and disinfectedseeds on agar medium,consisting of Nbasalelements,3％ sucrose,1000mg/L proline,and 32mg/L 2,4-D.After one month＇s in- duction culture and 3 wk subculture,compactand nodular calli were picked out and trans- ferred into liquid medium for suspension cul-tures.The liquid medium contained the same
