In order to screen the genes controlling watermelon rind color and luster, the experiment was carried out with yellow watermelon skin mutants as tester and green wild type watermelon as control, and transcriptome sequ...In order to screen the genes controlling watermelon rind color and luster, the experiment was carried out with yellow watermelon skin mutants as tester and green wild type watermelon as control, and transcriptome sequencing and bioinformatics analysis were done. The results show that 34.27 Gb clean data were got by transcriptome sequencing. There are 261 differentially expressed genes among Y_1_vs_G_1, Y_2_vs_G_2 and Y_3_vs_G_3. The pathways contenting most differentially expressed genes are plant hormone signal transduction pathway, phenylpropanoid biosynthesis pathway, photosynthesis pathway, starch and sucrose metabolism pathway. 9-cis-epoxycarotenoid dioxygenase(Cla002942), alcohol dehydrogenase(Cla004992), photosystem Ⅰ reaction center subunit Ⅲ, chloroplastic(precursor)(Cla009181), long-chain acyl coenzyme A synthetase(Cla017341), threonine dehydratase biosynthetic(Cla018352) candidates genes were screened out.展开更多
以西瓜浅绿色果皮、完全花品系ZXG1555及绿色果皮、单性花自交系Cream of Saskatchewan(简称“COS”)为亲本构建F_(2)群体,作BSA-seq分析,结合InDel和CAPS标记作基因分型,利用两亲本及其他6份绿色果皮、单性花西瓜品系基因组重测序数据...以西瓜浅绿色果皮、完全花品系ZXG1555及绿色果皮、单性花自交系Cream of Saskatchewan(简称“COS”)为亲本构建F_(2)群体,作BSA-seq分析,结合InDel和CAPS标记作基因分型,利用两亲本及其他6份绿色果皮、单性花西瓜品系基因组重测序数据,筛选并预测候选基因。结果表明,浅绿色果皮性状和单性花性状均由单隐性基因调控,利用分子标记筛选隐性性状单株重组事件,将控制两性状的关键基因分别定位于9号染色体1.1 Mb和3号染色体0.7 Mb区间内。筛选区间内候选基因,初步推测Cla97C09G175170(Two-component response regulator-like protein APRR2)和Cla97C03G066110(1-aminocyclopropane-1-carboxylate synthase 7)分别为西瓜浅绿色果皮和单性花性状候选基因。展开更多
基金Project(31260476)supported by the National Natural Science Foundation of China
文摘In order to screen the genes controlling watermelon rind color and luster, the experiment was carried out with yellow watermelon skin mutants as tester and green wild type watermelon as control, and transcriptome sequencing and bioinformatics analysis were done. The results show that 34.27 Gb clean data were got by transcriptome sequencing. There are 261 differentially expressed genes among Y_1_vs_G_1, Y_2_vs_G_2 and Y_3_vs_G_3. The pathways contenting most differentially expressed genes are plant hormone signal transduction pathway, phenylpropanoid biosynthesis pathway, photosynthesis pathway, starch and sucrose metabolism pathway. 9-cis-epoxycarotenoid dioxygenase(Cla002942), alcohol dehydrogenase(Cla004992), photosystem Ⅰ reaction center subunit Ⅲ, chloroplastic(precursor)(Cla009181), long-chain acyl coenzyme A synthetase(Cla017341), threonine dehydratase biosynthetic(Cla018352) candidates genes were screened out.
