A phosphate-accumulating bacteria strain PAO3-1 was isolated from biological phosphorus removal sludge supplied with sodium acetate as carbon source under stable performance. This strain has good enhanced biological p...A phosphate-accumulating bacteria strain PAO3-1 was isolated from biological phosphorus removal sludge supplied with sodium acetate as carbon source under stable performance. This strain has good enhanced biological phosphorus removal effect on normal activated sludge system. Phosphorus removal ratio was raised form 44% with no added strain to more than 82% with strain strengthening biological phosphorus removal. It is identified to be Alcaligenes sp. according to its morphology, biochemical characteristics and 16S rDNA sequence analysis. The cell of strain PAO3-1 is straight bacilli form, 0.4×1.1μm, no flagellum, gram negative and special aerobiotic. The optimal temperature and pH for growth are 32℃-37℃ and 5.5-9.5, respectively. The shape of slant clone is feathery. The phosphate accumulating rate of strain PAO3-1 was 8.1mgP/g cell·h, and 14.3 mgP/g cell·h when in phosphate-starving situation, which was 76.5% higher than that in non-starving situation. Its phosphate release rate of log course in anaerobic phase and in culture without phosphorus was 7.6mgP/g cell·h, while in stable course the rate was 6.1mgP/g cell·h. The rate in stable course was 19.7% lower than that in log course.展开更多
Absorption and catalytic oxidation of nitric oxide can be achieved by using cobalt（Ⅲ） ethylenediamine （Co（en）3^3＋. When simultaneous absorbing SO2 and NO, the precipitation of Co2（SO3）3 will be yielded and th...Absorption and catalytic oxidation of nitric oxide can be achieved by using cobalt（Ⅲ） ethylenediamine （Co（en）3^3＋. When simultaneous absorbing SO2 and NO, the precipitation of Co2（SO3）3 will be yielded and the NO removal will be decreased. A new catalyst system using Co（en）3^3＋ coupled with urea has been developed to simultaneous remove NO and SO2 in the flue gas. NO is absorbed and catalytically oxidized to nitrite and nitrate by Co（en）3^3＋. The dissolved oxygen in scrubbing solution from the feed stream acts as oxidant. Urea restrains the precipitation of Co2（SO3）3 by oxidizing SO3^2-to SO4^2- as COSO4 is more soluble in water. The experimental results proved that nearly all SO3^2- can be oxidized to SO4^2- and the high NO and SO2 removal could be obtained with the new system. The NO removal is influenced by gas flow rate, the concentration of Co（en）3^3＋ and urea in the absorption solution, the temperature of the scrubbing solution and the content of oxygen in the flue gas. The low gas flow rate is favorable to increase the NO removal. The experiments proved that the NO removal could be maintained at more than 95% by the system of 0.02 mol/L Co（en）3^3＋ and 1% urea at 50℃ with 10% O2 in the flue gas.展开更多
A new kind of bio-fluid bed used to treat dyes wastewater is described in detail due to its several special features,such as high removal efficiency,simple struc-ture,shock load resistance,etc.By means of analyzing th...A new kind of bio-fluid bed used to treat dyes wastewater is described in detail due to its several special features,such as high removal efficiency,simple struc-ture,shock load resistance,etc.By means of analyzing the experiment data,the results show that the dye wastewater’s organic matter is removed greatly after be-ing treated by this new kind of bio-fluid bed.On the other hand,the removal efficiency of chromaticity of展开更多
Optical microscopy, and scanning electron microscopy in conjunction with energy dispersed X-ray spectrometry （SEM-EDX）, have been used to study the minerals and the concentrations of 12 trace elements in the No.14 c...Optical microscopy, and scanning electron microscopy in conjunction with energy dispersed X-ray spectrometry （SEM-EDX）, have been used to study the minerals and the concentrations of 12 trace elements in the No.14 coal from the Huolinhe mine, Inner Mongolia China. The distribution, affinity and removability of the trace elements were studied by float-sink experiments and petrological methods. A high mineral content, dominated by clay minerals, was found in the No.14 coal from the Huolinhe mine. The concentrations of As, Sb and Hg are relatively high compared to the average values for Chinese coals. As, Cr, Hg, Li, Mn, Pb are mainly associated with the minerals while Cd, Co, Ni, Sb, and Se are evenly distributed between the minerals and the organic matter. Be and Ba are mainly distributed in the minerals with a minor proportion in the organic matter. Most elements have a low organic affinity, although Sb, Se, Co, Cd, Ni are closely integrated with the organic matter. High theoretical removabilities are indicated for most trace elements. So it may be possible to lower the concentrations of trace elements during coal preparation.展开更多
High nitrate(NO_3^-)loading in water bodies is a crucial factor inducing the eutrophication of lakes.We tried to enhance NO_3^-reduction in overlying water by coupling sediment microbial fuel cells(SMFCs)with submerge...High nitrate(NO_3^-)loading in water bodies is a crucial factor inducing the eutrophication of lakes.We tried to enhance NO_3^-reduction in overlying water by coupling sediment microbial fuel cells(SMFCs)with submerged aquatic plant Ceratophyllum demersum.A comparative study was conducted by setting four treatments:open-circuit SMFC(Control),closed-circuit SMFC(SMFC-c),open-circuit SMFC with C.demersum(Plant),and closed-circuit SMFC with C.demersum(P-SMFC-c).The electrochemical parameters were documented to illustrate the bio-electrochemical characteristics of SMFC-c and P-SMFC-c.Removal pathways of NO_3^- in different treatments were studied by adding quantitative^(15)NO_3^- to water column.The results showed that the cathodic reaction in SMFC-c was mainly catalyzed by aerobic organisms attached on the cathode,including algae,Pseudomonas,Bacillus,and Albidiferax.The oxygen secreted by plants significantly improved the power generation of SMFC-c.Both electrogenesis and plants enhanced the complete removal of NO_3^- from the sediment–water system.The complete removal rates of added^(15)N increased by 17.6% and 10.2% for SMFC-c and plant,respectively,when compared with control at the end of experiment.The electrochemical/heterotrophic and aerobic denitrification on cathodes mainly drove the higher reduction of NO_3^- in SMFC-c and plant,respectively.The coexistence of electrogenesis and plants further increased the complete removal of NO_3^- with a rate of 23.1%.The heterotrophic and aerobic denitrifications were simultaneously promoted with a highest abundance of Flavobacterium,Bacillus,Geobacter,Pseudomonas,Rhodobacter,and Arenimonas on the cathode.展开更多
A psychrophilic aerobic denitrifying bacterium,strain S1-1,was isolated from a biological aerated filter conducted for treatment of recirculating water in a marine aquaculture system.Strain S1-1 was preliminarily iden...A psychrophilic aerobic denitrifying bacterium,strain S1-1,was isolated from a biological aerated filter conducted for treatment of recirculating water in a marine aquaculture system.Strain S1-1 was preliminarily identified as Psychrobacter sp.based on the analysis of its 16S rRNA gene sequence,which showed 100% sequence similarity to that of Psychrobacter sp.TSBY-70.Strain S1-1 grew well either in high nitrate or high nitrite conditions with a removal of 100% nitrate or 63.50% nitrite,and the total nitrogen removal rates could reach to 46.48% and 31.89%,respectively.The results indicated that nitrate was mainly reduced in its logarithmic growth phase with a very low leve1 accumulation of nitrite,suggesting that the aerobic denitrification process of strain S1-1 occurred mainly in this phase.The GC-MS results showed that N 2 O was formed as the major intermediate during the aerobic denitrifying process of strain S1-1.Finally,factors affecting the growth of strain S1-1 and its aerobic denitrifying ability were also investigated.Results showed that the optimum aerobic denitrification conditions for strain S1-1 were sodium succinate as carbon source,C/N ratio15,salinity 10 g/L NaCl,incubation temperature 20°C and initial pH 6.5.展开更多
基金Fok Ying Tung Education Foundation ( No.94004)Shanghai Natural ScienceFoundation(No.04ZR14010)Young Teacher Foundation of Donghua University (No.113-10-0044065)
文摘A phosphate-accumulating bacteria strain PAO3-1 was isolated from biological phosphorus removal sludge supplied with sodium acetate as carbon source under stable performance. This strain has good enhanced biological phosphorus removal effect on normal activated sludge system. Phosphorus removal ratio was raised form 44% with no added strain to more than 82% with strain strengthening biological phosphorus removal. It is identified to be Alcaligenes sp. according to its morphology, biochemical characteristics and 16S rDNA sequence analysis. The cell of strain PAO3-1 is straight bacilli form, 0.4×1.1μm, no flagellum, gram negative and special aerobiotic. The optimal temperature and pH for growth are 32℃-37℃ and 5.5-9.5, respectively. The shape of slant clone is feathery. The phosphate accumulating rate of strain PAO3-1 was 8.1mgP/g cell·h, and 14.3 mgP/g cell·h when in phosphate-starving situation, which was 76.5% higher than that in non-starving situation. Its phosphate release rate of log course in anaerobic phase and in culture without phosphorus was 7.6mgP/g cell·h, while in stable course the rate was 6.1mgP/g cell·h. The rate in stable course was 19.7% lower than that in log course.
文摘Absorption and catalytic oxidation of nitric oxide can be achieved by using cobalt（Ⅲ） ethylenediamine （Co（en）3^3＋. When simultaneous absorbing SO2 and NO, the precipitation of Co2（SO3）3 will be yielded and the NO removal will be decreased. A new catalyst system using Co（en）3^3＋ coupled with urea has been developed to simultaneous remove NO and SO2 in the flue gas. NO is absorbed and catalytically oxidized to nitrite and nitrate by Co（en）3^3＋. The dissolved oxygen in scrubbing solution from the feed stream acts as oxidant. Urea restrains the precipitation of Co2（SO3）3 by oxidizing SO3^2-to SO4^2- as COSO4 is more soluble in water. The experimental results proved that nearly all SO3^2- can be oxidized to SO4^2- and the high NO and SO2 removal could be obtained with the new system. The NO removal is influenced by gas flow rate, the concentration of Co（en）3^3＋ and urea in the absorption solution, the temperature of the scrubbing solution and the content of oxygen in the flue gas. The low gas flow rate is favorable to increase the NO removal. The experiments proved that the NO removal could be maintained at more than 95% by the system of 0.02 mol/L Co（en）3^3＋ and 1% urea at 50℃ with 10% O2 in the flue gas.
文摘A new kind of bio-fluid bed used to treat dyes wastewater is described in detail due to its several special features,such as high removal efficiency,simple struc-ture,shock load resistance,etc.By means of analyzing the experiment data,the results show that the dye wastewater’s organic matter is removed greatly after be-ing treated by this new kind of bio-fluid bed.On the other hand,the removal efficiency of chromaticity of
文摘Optical microscopy, and scanning electron microscopy in conjunction with energy dispersed X-ray spectrometry （SEM-EDX）, have been used to study the minerals and the concentrations of 12 trace elements in the No.14 coal from the Huolinhe mine, Inner Mongolia China. The distribution, affinity and removability of the trace elements were studied by float-sink experiments and petrological methods. A high mineral content, dominated by clay minerals, was found in the No.14 coal from the Huolinhe mine. The concentrations of As, Sb and Hg are relatively high compared to the average values for Chinese coals. As, Cr, Hg, Li, Mn, Pb are mainly associated with the minerals while Cd, Co, Ni, Sb, and Se are evenly distributed between the minerals and the organic matter. Be and Ba are mainly distributed in the minerals with a minor proportion in the organic matter. Most elements have a low organic affinity, although Sb, Se, Co, Cd, Ni are closely integrated with the organic matter. High theoretical removabilities are indicated for most trace elements. So it may be possible to lower the concentrations of trace elements during coal preparation.
基金supported by the National Key Research and Development Plan of China (No. 2016YFC0500403-03)the Science and Technology Service Network Initiative of the Chinese Academy of Sciences (No. KFJ-STS-ZDTP-038)
文摘High nitrate(NO_3^-)loading in water bodies is a crucial factor inducing the eutrophication of lakes.We tried to enhance NO_3^-reduction in overlying water by coupling sediment microbial fuel cells(SMFCs)with submerged aquatic plant Ceratophyllum demersum.A comparative study was conducted by setting four treatments:open-circuit SMFC(Control),closed-circuit SMFC(SMFC-c),open-circuit SMFC with C.demersum(Plant),and closed-circuit SMFC with C.demersum(P-SMFC-c).The electrochemical parameters were documented to illustrate the bio-electrochemical characteristics of SMFC-c and P-SMFC-c.Removal pathways of NO_3^- in different treatments were studied by adding quantitative^(15)NO_3^- to water column.The results showed that the cathodic reaction in SMFC-c was mainly catalyzed by aerobic organisms attached on the cathode,including algae,Pseudomonas,Bacillus,and Albidiferax.The oxygen secreted by plants significantly improved the power generation of SMFC-c.Both electrogenesis and plants enhanced the complete removal of NO_3^- from the sediment–water system.The complete removal rates of added^(15)N increased by 17.6% and 10.2% for SMFC-c and plant,respectively,when compared with control at the end of experiment.The electrochemical/heterotrophic and aerobic denitrification on cathodes mainly drove the higher reduction of NO_3^- in SMFC-c and plant,respectively.The coexistence of electrogenesis and plants further increased the complete removal of NO_3^- with a rate of 23.1%.The heterotrophic and aerobic denitrifications were simultaneously promoted with a highest abundance of Flavobacterium,Bacillus,Geobacter,Pseudomonas,Rhodobacter,and Arenimonas on the cathode.
基金supported by the Knowledge Innova-tion Program of the Chinese Academy of Sciences(No.KJCX2-YW-L08)
文摘A psychrophilic aerobic denitrifying bacterium,strain S1-1,was isolated from a biological aerated filter conducted for treatment of recirculating water in a marine aquaculture system.Strain S1-1 was preliminarily identified as Psychrobacter sp.based on the analysis of its 16S rRNA gene sequence,which showed 100% sequence similarity to that of Psychrobacter sp.TSBY-70.Strain S1-1 grew well either in high nitrate or high nitrite conditions with a removal of 100% nitrate or 63.50% nitrite,and the total nitrogen removal rates could reach to 46.48% and 31.89%,respectively.The results indicated that nitrate was mainly reduced in its logarithmic growth phase with a very low leve1 accumulation of nitrite,suggesting that the aerobic denitrification process of strain S1-1 occurred mainly in this phase.The GC-MS results showed that N 2 O was formed as the major intermediate during the aerobic denitrifying process of strain S1-1.Finally,factors affecting the growth of strain S1-1 and its aerobic denitrifying ability were also investigated.Results showed that the optimum aerobic denitrification conditions for strain S1-1 were sodium succinate as carbon source,C/N ratio15,salinity 10 g/L NaCl,incubation temperature 20°C and initial pH 6.5.
