期刊文献+
共找到627,988篇文章
< 1 2 250 >
每页显示 20 50 100
Dynamic response and failure process of horizontal-layered fractured structure rock slope under strong earthquake 被引量:1
1
作者 WANG Tong LIU Xianfeng +5 位作者 HOU Zhaoxu XU Jiahang ZHANG Jun YUAN Shengyang JIANG Guanlu HU Jinshan 《Journal of Mountain Science》 SCIE CSCD 2024年第3期882-900,共19页
Rock slope with horizontal-layered fractured structure(HLFS)has high stability in its natural state.However,a strong earthquake can induce rock fissure expansion,ultimately leading to slope failure.In this study,the d... Rock slope with horizontal-layered fractured structure(HLFS)has high stability in its natural state.However,a strong earthquake can induce rock fissure expansion,ultimately leading to slope failure.In this study,the dynamic response,failure mode,and spectral characteristics of rock slope with HLFS under strong earthquake conditions were investigated based on the large-scale shaking table model test.On this basis,multiple sets of numerical calculation models were further established by UDEC discrete element program.Five influencing factors were considered in the parametric study of numerical simulations,including slope height,slope angle,bedding-plane spacing and secondary joint spacing as well as bedrock dip angle.The results showed that the failure process of rock slope with HLFS under earthquake action is mainly divided into four phases,i.e.,the tensile crack of the slope shoulder joints and shear dislocation at the top bedding plane,the extension of vertical joint cracks and increase of shear displacement,the formation of step-through sliding surfaces and the instability,and finally collapse of fractured rock mass.The acceleration response of slopes exhibits elevation amplification effect and surface effect.Numerical simulations indicate that the seismic stability of slopes with HLFS exhibits a negative correlation with slope height and angle,but a positive correlation with bedding-plane spacing,joint spacing,and bedrock dip angle.The results of this study can provide a reference for seismic stability evaluation of weathered rock slopes. 展开更多
关键词 Seismic behavior Horizontal layered Weathered rock slope Shaking table test Failure mode
下载PDF
Damage evolution of rock-encased-backfill structure under stepwise cyclic triaxial loading 被引量:1
2
作者 Xin Yu Yuye Tan +4 位作者 Weidong Song John Kemeny Shengwen Qi Bowen Zheng Songfeng Guo 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第2期597-615,共19页
Rock-encased-backfill(RB)structures are common in underground mining,for example in the cut-andfill and stoping methods.To understand the effects of cyclic excavation and blasting activities on the damage of these RB ... Rock-encased-backfill(RB)structures are common in underground mining,for example in the cut-andfill and stoping methods.To understand the effects of cyclic excavation and blasting activities on the damage of these RB structures,a series of triaxial stepwise-increasing-amplitude cyclic loading experiments was conducted with cylindrical RB specimens(rock on outside,backfill on inside)with different volume fractions of rock(VF=0.48,0.61,0.73,and 0.84),confining pressures(0,6,9,and 12 MPa),and cyclic loading rates(200,300,400,and 500 N/s).The damage evolution and meso-crack formation during the cyclic tests were analyzed with results from stress-strain hysteresis loops,acoustic emission events,and post-failure X-ray 3D fracture morphology.The results showed significant differences between cyclic and monotonic loadings of RB specimens,particularly with regard to the generation of shear microcracks,the development of stress memory and strain hardening,and the contact forces and associated friction that develops along the rock-backfill interface.One important finding is that as a function of the number of cycles,the elastic strain increases linearly and the dissipated energy increases exponentially.Also,compared with monotonic loading,the cyclic strain hardening characteristics are more sensitive to rising confining pressures during the initial compaction stage.Another finding is that compared with monotonic loading,more shear microcracks are generated during every reloading stage,but these microcracks tend to be dispersed and lessen the likelihood of large shear fracture formation.The transition from elastic to plastic behavior varies depending on the parameters of each test(confinement,volume fraction,and cyclic rate),and an interesting finding was that the transformation to plastic behavior is significantly lower under the conditions of 0.73 rock volume fraction,400 N/s cyclic loading rate,and 9 MPa confinement.All the findings have important practical implications on the ability of backfill to support underground excavations. 展开更多
关键词 rock and backfill Triaxial cyclic loading Volume fraction Damage evolution 3D visualization
下载PDF
Effect of high-multiple water injection on rock pore structure and oil displacement efficiency
3
作者 Xiao Lei Chunpeng Zhao +2 位作者 Qiaoliang Zhang Panrong Wang Runfu Xiong 《Energy Geoscience》 EI 2024年第1期234-238,共5页
Experimental methods,including mercury pressure,nuclear magnetic resonance(NMR)and core(wateroil)displacement,are used to examine the effects of high-multiple water injection(i.e.water injection with high injected por... Experimental methods,including mercury pressure,nuclear magnetic resonance(NMR)and core(wateroil)displacement,are used to examine the effects of high-multiple water injection(i.e.water injection with high injected pore volume)on rock properties,pore structure and oil displacement efficiency of an oilfield in the western South China Sea.The results show an increase in the permeability of rocks along with particle migration,an increase in the pore volume and the average pore throat radius,and enhanced heterogeneity after high-multiple water injection.Compared with normal water injection methods,a high-multiple water injection is more effective in improving the oil displacement efficiency.The degree of recovery increases faster in the early stage due to the expansion of the swept area,and the transition from oil-wet to water-wet.The degree of recovery increases less in the late stage due to various factors,including the enhancement of heterogeneity in the rocks.Considering both the economic aspect and the production limit of water flooding,it is recommended to adopt other technologies to further enhance oil recovery after 300 PV water injection. 展开更多
关键词 High multiple Water injection rock permeability Pore structure Oil displacement efficiency
下载PDF
Frost deformation and microstructure evolution of porous rock under uniform and unidirectional freeze-thaw conditions
4
作者 LV Zhitao LIU Jintao +1 位作者 WAN Ling LIU Weiping 《Journal of Mountain Science》 SCIE CSCD 2024年第8期2855-2869,共15页
The frost deterioration and deformation of porous rock are commonly investigated under uniform freeze-thaw(FT)conditions.However,the unidirectional FT condition,which is also prevalent in engineering practice,has rece... The frost deterioration and deformation of porous rock are commonly investigated under uniform freeze-thaw(FT)conditions.However,the unidirectional FT condition,which is also prevalent in engineering practice,has received limited attention.Therefore,a comparative study on frost deformation and microstructure evolution of porous rock under both uniform and unidirectional FT conditions was performed.Firstly,frost deformation experiments of rock were conducted under cyclic uniform and unidirectional FT action,respectively.Results illustrate that frost deformation of saturated rock exhibits isotropic characteristics under uniform FT cycles,while it shows anisotropic characteristics under unidirectional FT condition with both the frost heaving strain and residual strain along FT direction much higher than those perpendicular to FT direction.Moreover,the peak value and residual value of cumulative frost strain vary as logarithmic functions with cycle number under both uniform and unidirectional FT conditions.Subsequently,the microstructure evolution of rock suffered cyclic uniform and unidirectional FT action were measured.Under uniform FT cycles,newly generated pores uniformly distribute in rock and pore structure of rock remains isotropic in micro scale,and thus the frost deformation shows isotropic characteristics in macro scale.Under unidirectional FT cycles,micro-cracks or pore belts generate with their orientation nearly perpendicular to the FT direction,and rock structure gradually becomes anisotropic in micro scale,resulting in the anisotropic characteristics of frost deformation in macro scale. 展开更多
关键词 Frost deformation Microstructure evolution Porous rock Unidirectional freeze-thaw cycles Uniform freeze-thaw cycles
下载PDF
SLC9A3-AS1调控miR-148a-3p/ROCK1信号轴影响肾癌细胞生物学功能 被引量:1
5
作者 向威 吕磊 +2 位作者 郑福鑫 章传华 袁敬东 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第2期161-167,共7页
目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9... 目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9A3-AS1在ccRCC组织中的表达水平;采用实时荧光定量聚合酶链反应(qRT-PCR)检测SLC9A3-AS1在不同肾癌细胞系、24例ccRCC组织与癌旁正常肾脏组织中的表达水平;应用细胞增殖/毒性检测试剂盒(cell counting kit-8,CCK-8)和Transwell小室迁移实验检测敲低SLC9A3-AS1表达对肾癌细胞增殖与迁移的影响;应用蛋白质免疫印迹法检测增殖与迁移相关信号通路蛋白的表达水平;采用双荧光素酶报告基因实验验证SLC9A3-AS1与miR-148a-3p/ROCK1轴的靶向调控关系。结果GEPIA2软件分析结果显示,相较正常肾脏组织,SLC9A3-AS1在肾透明细胞癌组织中表达显著上调(P<0.01)。qRT-PCR结果显示,相较癌旁正常肾脏组织,SLC9A3-AS1在24例ccRCC组织中表达显著上调(P<0.01);相较永生化肾小管上皮细胞,SLC9A3-AS1在4种肾癌细胞系中表达均显著上调,以786-O细胞最为显著(P<0.01)。干扰SLC9A3-AS1表达,可显著抑制786-O细胞的增殖与迁移能力,上调E-cadherin的蛋白表达水平,而下调N-cadherin、MMP2的蛋白表达水平(均P<0.05);过表达miR-148a-3p可显著抑制786-O细胞的增殖与迁移能力(P<0.01)。双荧光素酶报告基因检测结果表明,SLC9A3-AS1可特异性结合miR-148a-3p,后者进一步靶向结合ROCK1 mRNA的3′UTR区域;过表达miR-148a-3p可明显下调ROCK1 mRNA的表达水平,敲低miR-148a-3p表达则产生相反的效应;敲低SLC9A3-AS1表达可显著下调786-O细胞中ROCK1 mRNA与蛋白的表达水平(P<0.01);下调miR-148a-3p表达可部分逆转SLC9A3-AS1沉默对786-O细胞增殖与迁移的抑制作用(P<0.05)。结论SLC9A3-AS1在ccRCC中通过调控miR-148a-3p/ROCK1轴发挥促癌作用,有望成为ccRCC的一个新的分子标志物。 展开更多
关键词 肾透明细胞癌 SLC9A3-AS1 miR-148a-3p rock1
下载PDF
贝母素乙调节RhoA/ROCK信号通路对膝骨关节炎大鼠软骨损伤的影响
6
作者 彭博文 冯立 覃剑 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第7期980-984,共5页
目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组... 目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组、贝母素乙(3.5 mg/kg)+LPA(RhoA激活剂,1 mg/kg)组,每组10只,另选10只大鼠为对照组。机械刺激法和热辐射法检测大鼠膝关节疼痛症状;检测大鼠关节肿胀度、膝关节宽度并以步态评分评测其关节功能;透射电镜检测大鼠膝关节软骨细胞超微结构;TUNEL染色检测大鼠关节软骨细胞凋亡比;酶联免疫吸附(ELISA)法检测大鼠血清和关节液促炎因子白细胞介素(IL)-18、单核细胞趋化蛋白1(MCP-1)水平;免疫印迹检测各组大鼠关节软骨组织凋亡(Cleaved caspase-3、Bax)和RhoA/ROCK通路相关蛋白表达。结果 贝母素乙可降低KOA大鼠关节肿胀度、膝关节宽度、步态评分、软骨细胞凋亡比、血清及关节液IL-18、MCP-1水平、软骨组织Cleaved caspase-3、Bax、RhoA、ROCK1、ROCK1蛋白表达,升高机械痛阈值、热痛阈值(P<0.05);LPA可减弱贝母素乙对KOA大鼠关节软骨损伤的改善作用。结论 贝母素乙可通过抑制RhoA/ROCK信号激活而抑制KOA大鼠关节炎症反应和关节软骨细胞凋亡,减轻软骨损伤。 展开更多
关键词 贝母素乙 RhoA/rock 膝骨关节炎 软骨损伤 信号通路
下载PDF
桃叶珊瑚苷调节RhoA/ROCK信号通路对胶质母细胞瘤细胞活力和上皮间质转化的影响
7
作者 李娟 石海平 李维民 《天津中医药》 CAS 2024年第3期386-392,共7页
[目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(C... [目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(CCK-8)法检测细胞活力,流式细胞术检测细胞凋亡,Transwell小室实验检测细胞迁移和侵袭,蛋白免疫印迹法(Western Blot)检测基质金属蛋白酶(MMP)2、MMP9、波形蛋白(Vimentin)、上皮钙黏蛋白(E-cadherin)、神经钙黏蛋白(N-cadherin)、Ras同源基因家族成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶(ROCK)1、ROCK2表达。构建GBM裸鼠模型,随机分为裸鼠对照组、AU组、Y-27632组、AU+Y-27632组,测量肿瘤质量与体积,免疫组化法检测移植瘤组织RhoA、ROCK1、ROCK2蛋白表达。[结果]GBM细胞活力随着AU浓度的升高而逐渐降低(P<0.05),选择U87作为后续实验细胞,选择10、25、50μmol/L浓度作为AU后续实验浓度。与对照组比较,AU低、中、高浓度组和Y-27632组细胞活力、迁移和侵袭细胞数、MMP2、MMP9、N-cadherin、Vimentin、RhoA、ROCK1、ROCK2表达显著下降,凋亡率、E-cadherin表达显著升高(P<0.05),其中高浓度AU和Y-27632组共同处理的细胞变化更显著(P<0.05)。AU和Y-27632均能抑制移植瘤质量和体积,降低RhoA/ROCK信号通路蛋白表达(P<0.05)。[结论]AU能抑制GBM细胞活力、迁移侵袭和EMT,促进细胞凋亡,其作用机制可能与抑制RhoA/ROCK信号通路有关。 展开更多
关键词 胶质母细胞瘤 细胞活力 上皮间质转化 桃叶珊瑚苷 RhoA/rock信号通路
下载PDF
Numerical investigation of the mechanical behavior of the backfill–rock composite structure under triaxial compression 被引量:5
8
作者 Hongjian Lu Yiren Wang +2 位作者 Deqing Gan Jie Wu Xiaojun Wu 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2023年第5期802-812,共11页
To ensure safe and economical backfill mining,the mechanical response of the backfill–rock interaction system needs to be understood.The numerical investigation of the mechanical behavior of backfill–rock composite ... To ensure safe and economical backfill mining,the mechanical response of the backfill–rock interaction system needs to be understood.The numerical investigation of the mechanical behavior of backfill–rock composite structure(BRCS)under triaxial compression,which includes deformation,failure patterns,strength characteristics,and acoustic emission(AE)evolution,was proposed.The models used in the tests have one rough interface,two cement–iron tailings ratios(CTRs),four interface angles(IAs),and three confining pressures(CPs).Results showed that the deformation,strength characteristics,and failure patterns of BRCS under triaxial compression depend on IA,CP,and CTR.The stress–strain curves of BRCS under triaxial compression could be divided into five stages,namely,compaction,elasticity,yield,strain softening,and residual stress.The relevant AE counts have corresponding relationships with different stages.The triaxial compressive strengths of composites increase linearly with the increase of the CP.Furthermore,the CP stress strengthening effect occurs.When the IAs are45°and 60°,the failure areas of composites appear in the interface and backfill.When the IAs are 75°and 90°,the failure areas of composites appear in the backfill,interface,and rock.Moreover,the corresponding failure modes yield the combined shear failure.The research results provide the basis for further understanding of the stability of the BRCS. 展开更多
关键词 backfill–rock composite structure triaxial compression mechanical behavior acoustic emission numerical simulation
下载PDF
芍药苷调节RhoA/ROCK信号通路对实验性自身免疫性葡萄膜炎小鼠Th17/Treg免疫平衡的影响 被引量:2
9
作者 庞彬彬 陈震 邢怡桥 《中药新药与临床药理》 CAS CSCD 北大核心 2024年第4期506-512,共7页
目的探讨芍药苷调节Ras同源基因家族蛋白A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)小鼠辅助性T淋巴细胞17(Th17)/调节性T细胞(Treg)免疫平衡的影响。方法随... 目的探讨芍药苷调节Ras同源基因家族蛋白A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)小鼠辅助性T淋巴细胞17(Th17)/调节性T细胞(Treg)免疫平衡的影响。方法随机选出10只小鼠作为正常对照组,其余小鼠构建EAU模型,将造模成功小鼠随机平分为模型组、芍药苷组(10 mg·kg^(-1)芍药苷)、RhoA/ROCK信号通路激活剂溶血磷脂酸(LPA)组(25μmol·L^(-1)LPA)、芍药苷+LPA组(10 mg·kg^(-1)芍药苷+25μmol·L^(-1)LPA),每组10只。模型组和正常对照组给予等量生理盐水,给药每天1次,持续14 d。眼前节临床表现评分评估炎症严重程度;HE染色观察眼球组织病理形态;ELISA法检测血清中炎性因子转化生长因子-β(trarsforming growth factor beta,TGF-β)、白细胞介素10(IL-10)、白细胞介素17(IL-17)、白细胞介素23(IL-23)水平;流式细胞术检测脾脏Th17/Treg细胞水平;Western Blot法检测维甲酸相关核孤儿受体γt(RORγt)、叉头状转录因子3(Foxp3)以及RhoA/ROCK信号通路蛋白表达。结果正常对照组小鼠无虹膜、结膜血管舒张充血,与正常对照组比,模型组小鼠出现虹膜血管舒张充血,前房、虹膜、睫状体和视网膜内均有大量炎性细胞膨胀,眼前节临床表现评分、IL-17和IL-23含量、Th17细胞水平、Th17/Treg、RORγt、RhoA、ROCK1蛋白水平明显升高(P<0.05),IL-10和TGF-β含量、Treg细胞水平、Foxp3水平明显下降(P<0.05)。与模型组比,芍药苷组小鼠虹膜血管充血症状明显缓解,眼前节临床表现评分、IL-17和IL-23含量、Th17细胞水平、Th17/Treg、RORγt、RhoA、ROCK1蛋白水平明显降低(P<0.05),IL-10和TGF-β含量、Treg细胞水平、Foxp3水平明显升高(P<0.05);而LPA加重了眼部损伤(P<0.05),逆转了芍药苷对EAU小鼠的改善效果(P<0.05)。结论芍药苷可通过下调RhoA/ROCK信号通路调节EAU小鼠Th17/Treg免疫平衡减轻EAU小鼠葡萄膜炎。 展开更多
关键词 芍药苷 RhoA/rock信号通路 自身免疫性葡萄膜炎 TH17/TREG 小鼠
下载PDF
苍术素调节RhoA/ROCK信号通路对弥漫大B细胞淋巴瘤细胞恶性生物学行为的影响 被引量:1
10
作者 张薇 陈姣敏 +2 位作者 许卫星 尹凤雷 王娟 《陕西医学杂志》 CAS 2024年第5期593-597,共5页
目的:探讨苍术素(ATR)调节Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对弥漫大B细胞淋巴瘤(DLBCL)细胞恶性生物学行为的影响。方法:常规培养DLBCL细胞SUDHL-4,将其随机分为对照组、低浓度ATR组(ATR-L组,5μ... 目的:探讨苍术素(ATR)调节Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对弥漫大B细胞淋巴瘤(DLBCL)细胞恶性生物学行为的影响。方法:常规培养DLBCL细胞SUDHL-4,将其随机分为对照组、低浓度ATR组(ATR-L组,5μmol/L ATR)、中浓度ATR组(ATR-M组,10μmol/L ATR)、高浓度ATR组(ATR-H组,20μmol/L ATR)和高浓度ATR+RhoA激活剂U46619组(ATR+U46619组,20μmol/L ATR+10 nmol/L U46619)。CCK-8法测定细胞增殖活性。划痕实验检测细胞迁移能力。Transwell实验测定细胞侵袭能力。流式细胞术检测细胞凋亡率。实时荧光定量PCR(RT-qPCR)法测定细胞中RhoA、ROCK1 mRNA表达。免疫印迹法(Western blot)测定各组细胞B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、RhoA、ROCK1蛋白表达。结果:与对照组比较,ATR-M组、ATR-H组SUDHL-4细胞OD450值(48、72 h)、细胞迁移率、细胞侵袭数目、RhoA、ROCK1 mRNA和蛋白表达、Bcl-2蛋白表达降低,细胞凋亡率、Bax蛋白表达升高(均P<0.05)。RhoA激活剂U46619减弱了ATR对DLBCL细胞恶性生物学行为的抑制作用。结论:ATR可能通过下调RhoA/ROCK信号通路抑制DLBCL细胞恶性生物学行为。 展开更多
关键词 弥漫大B细胞淋巴瘤 苍术素 RhoA/rock通路 细胞凋亡 细胞增殖 细胞侵袭 细胞迁移
下载PDF
艾灸联合保真汤加减治疗糖尿病肾病的疗效观察及对CT灌注参数、Rho/ROCK信号通路蛋白的影响 被引量:1
11
作者 赵小艳 王俊杰 《上海针灸杂志》 CSCD 2024年第1期17-23,共7页
目的 观察艾灸联合保真汤加减治疗糖尿病肾病气阴两虚证的临床疗效及对CT灌注参数、Rho/ROCK信号通路蛋白的影响。方法 将146例糖尿病肾病气阴两虚证患者随机分为观察组(74例)和对照组(72例)。在对症治疗的基础上,对照组给予厄贝沙坦片... 目的 观察艾灸联合保真汤加减治疗糖尿病肾病气阴两虚证的临床疗效及对CT灌注参数、Rho/ROCK信号通路蛋白的影响。方法 将146例糖尿病肾病气阴两虚证患者随机分为观察组(74例)和对照组(72例)。在对症治疗的基础上,对照组给予厄贝沙坦片,观察组给予艾灸联合保真汤加减。观察两组中医主症积分、CT灌注参数[血尿素氮(blood urea nitrogen, BUN)、血肌酐(serum creatinine, SCr)、尿白蛋白排泄率(urinary albumin excretion rate, UAER)和24 h尿蛋白定量(24-hour urinary protein quantification, 24 h Upro)]、肾血流指标[舒张末期血流速度(end-diastolic velocity, EDV)、肾段动脉的收缩期峰值流速(peak-systolic velocity, PSV)、搏动指数(pulsatility index, PI)和阻力指数(resistive index, RI)]、血清炎性因子[肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、白细胞介素-1β(interleukin-1β, IL-1β)、白细胞介素-6(interleukin-6, IL-6)和C反应蛋白(C-reactive protein, CRP)]水平及Rho/ROCK信号通路[Ras同源基因家族成员A(Ras homolog gene family member A, RhoA)、Rho关联含卷曲螺旋蛋白激酶Ⅰ(Rho-associated coiled-coil containing protein kinaseⅠ, ROCKI)、α-平滑肌肌动蛋白(α-smooth muscle actin, α-SMA)和钙黏附蛋白-E(E-cadherin, E-Cad)]蛋白水平,并比较两组临床疗效及不良反应。结果 观察组总有效率为97.3%(72/74),明显高于对照组的81.9%(59/72)(P<0.05)。观察组治疗后中医主症积分低于治疗前和对照组(P<0.05)。两组治疗后CT灌注参数降低(P<0.05),且观察组低于对照组(P<0.05)。观察组治疗后PSV、EDV较治疗前和对照组加快(P<0.05),RI、PI较治疗前和对照组降低(P<0.05)。观察组治疗后血清炎性因子水平较治疗前和对照组降低(P<0.05)。观察组治疗后Rho A、ROCKI、α-SMA蛋白水平较治疗前和对照组降低(P<0.05),E-Cad蛋白水平较治疗前和对照组升高(P<0.05)。观察组不良反应发生率为1.4%(1/74),低于对照组的19.4%(14/72)(P<0.05)。结论 在对症治疗的基础上,艾灸联合保真汤加减可明显提高糖尿病肾病气阴两虚证患者的治疗效果,其机制可能与改善CT灌注参数,调节血清Rho/ROCK信号通路蛋白相关。 展开更多
关键词 灸法 糖尿病并发症 糖尿病肾病 气阴两虚 Rho/rock信号通路蛋白
下载PDF
黄芩苷通过ROS依赖性调节RhoA/ROCK通路保护氯化钴诱导心肌细胞损伤的实验研究 被引量:2
12
作者 沈艳玲 刘承红 +3 位作者 王世魁 徐尧 张云波 顾申红 《海南医学院学报》 CAS 北大核心 2024年第7期481-488,共8页
目的:本研究旨在探讨黄芩苷(Baicalin)对氯化钴(CoCl_(2))诱导H9c2心肌细胞缺氧损伤的作用机制。方法:采用CoCl_(2)建立心肌细胞缺氧损伤模型,并分别加入不同浓度的黄芩苷培养。将正常氧培养设置为对照组,CoCl_(2)培养H9c2心肌细胞设置... 目的:本研究旨在探讨黄芩苷(Baicalin)对氯化钴(CoCl_(2))诱导H9c2心肌细胞缺氧损伤的作用机制。方法:采用CoCl_(2)建立心肌细胞缺氧损伤模型,并分别加入不同浓度的黄芩苷培养。将正常氧培养设置为对照组,CoCl_(2)培养H9c2心肌细胞设置为CoCl_(2)组;Baicalin、Y27632(Rho激酶抑制剂)预处理的H9c2缺氧心肌细胞设置为CoCl_(2)+Baicalin组、CoCl_(2)+Y27632组、CoCl_(2)+Baicalin+Y27632组。通过细胞毒性检测试剂盒(CCK8)检测细胞活性;荧光探针测定细胞中活性氧(ROS)的表达;WST-8检测心肌细胞超氧化歧化酶(SOD)活力;TBA法测定丙二醇(MDA)浓度;同时采用WesternBlot方法分析RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达情况。结果:处理H9c2细胞24 h后,1 000μmol/L的CoCl_(2)和75μmol/L黄芩苷对治疗心肌细胞缺氧损伤具有较好的细胞活力。CoCl_(2)组细胞活性明显低于对照组,加入黄芩苷后可显著提高细胞活性(P<0.05);与对照组相比,CoCl_(2)组心肌细胞可上调ROS、MDA表达,下调SOD活力,升高RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达水平,加入Baicalin后可逆转上诉蛋白的表达水平;与CoCl_(2)组相比,CoCl_(2)+Baicalin可抑制ROS、MDA的表达,上调SOD含量,下调RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达水平,而加入Y27632(Rho激酶抑制剂)后则可显著增强Baicalin带来的保护作用。结论:Baicalin可减轻CoCl_(2)诱导H9c2心肌细胞缺氧损伤的炎症、氧化应激反应,其机制与抑制ROS依赖性RhoA/ROCK通路有关。 展开更多
关键词 黄芩苷 RhoA/Rho相关激酶(rock)信号通路 缺氧损伤模型
下载PDF
川芎嗪抑制ROCK的表达降低模拟失重大鼠血管Ca^(2+)敏感性
13
作者 王慧平 白晓镯 +4 位作者 赵晶 赵省心 刘朕印 党凯 高云芳 《西北大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第5期919-928,共10页
研究失重条件下血管平滑肌收缩性、Ca^(2+)敏感性及其调控通路RhoA-ROCK蛋白表达的变化,以及川芎嗪干预对其的影响。大鼠尾部悬吊模拟失重,在机体前、后部分别选取颈总动脉和肠系膜上动脉。在模拟失重大鼠颈总动脉中,由苯肾上腺素(PHE)... 研究失重条件下血管平滑肌收缩性、Ca^(2+)敏感性及其调控通路RhoA-ROCK蛋白表达的变化,以及川芎嗪干预对其的影响。大鼠尾部悬吊模拟失重,在机体前、后部分别选取颈总动脉和肠系膜上动脉。在模拟失重大鼠颈总动脉中,由苯肾上腺素(PHE)或KCl诱发的血管收缩性和Ca^(2+)敏感性增强,RhoA激酶2(ROCK II)的表达、肌球蛋白磷酸酶靶亚基1(MYPT1)和肌球蛋白调节轻链(MLC)的磷酸化水平均上升,血管孵育Y-27632(ROCK特异性抑制剂)后可降低以上变化。模拟失重大鼠灌饲川芎嗪亦可降低以上变化。模拟失重后,大鼠肠系膜上动脉的收缩性和Ca^(2+)敏感性、ROCK II的表达、MYPT1和MLC的磷酸化水平降低,血管孵育Y-27632对以上变化无明显作用。模拟失重大鼠灌饲川芎嗪亦对以上变化无明显作用。结果表明,由RhoA-ROCK调控的血管平滑肌Ca^(2+)敏感性的变化可能是失重条件下机体前后部血管收缩性发生区域性重塑的关键因素。川芎嗪可抑制ROCK蛋白的表达,降低血管平滑肌升高的Ca^(2+)敏感性,从而纠正失重条件下机体前部血管收缩性的增强,但对失重条件下机体后部血管收缩性的减弱无恢复作用。 展开更多
关键词 Ca^(2+)敏感性 RhoA-rock 血管收缩性 模拟失重 川芎嗪
下载PDF
Geostructures, dynamics and risk mitigation of high-altitude and long- runout rockslides 被引量:7
14
作者 Yueping Yin Bin Li +3 位作者 Yang Gao Wenpei Wang Shilin Zhang Nan Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2023年第1期66-101,共36页
Long-runout rockslides at high altitude could cause disaster chain in river basins and destroy towns and major infrasturctures.This paper firstly explores the initiation mechanism of high-altitude and long-runout rock... Long-runout rockslides at high altitude could cause disaster chain in river basins and destroy towns and major infrasturctures.This paper firstly explores the initiation mechanism of high-altitude and long-runout rockslides.Two types of sliding-prone geostructure models,i.e.the fault control type in orogenic belt and the fold control type in platform area,are proposed.Then,large-scale experimental apparatus and associated numerical simulations are conducted to understanding the chain-style dynamics of rockslide-debris avalanche-debris flow.The results reveal the fragmentation effects,the rheological behaviors and the boundary layer effect of long-runout avalanche-debris flow.The dynamic character-istics of quasi-static-transition-inertia state and solid-liquid coupling in rapid movement of rockslide-debris avalanche-debris flow are investigated.Finally,the risk mitigation strategy of the non-structure and structure for resilient energy dissipation are illustrated for initiation,transition and deposition zones.The structural prevention and mitigation methods have been successfully applied to the high-altitude and long-runout rockslides in Zhouqu and Maoxian of the Wenchuan earthquake zone,as well as the other major geohazards in Qinghai-Tibet Plateau and its adjacent areas. 展开更多
关键词 rockSLIDE High-altitude and long-runout Sliding-prone geostructure Large-scale experimental apparatus Risk mitigation strategy Structural prevention technique
下载PDF
Mineralogy,microstructures and geomechanics of rock salt for underground gas storage 被引量:2
15
作者 Veerle Vandeginste Yukun Ji +1 位作者 Frank Buysschaert George Anoyatis 《Deep Underground Science and Engineering》 2023年第2期129-147,共19页
Rock salt has excellent properties for its use as underground leak‐proof containers for the storage of renewable energy.Salt solution mining has long been used for salt mining,and can now be employed in the construct... Rock salt has excellent properties for its use as underground leak‐proof containers for the storage of renewable energy.Salt solution mining has long been used for salt mining,and can now be employed in the construction of underground salt caverns for the storage of hydrogen gas.This paper presents a wide range of methods to study the mineralogy,geochemistry,microstructure and geomechanical characteristics of rock salt,which are important in the engineering of safe underground storage rock salt caverns.The mineralogical composition of rock salt varies and is linked to its depositional environment and diagenetic alterations.The microstructure in rock salt is related to cataclastic deformation,diffusive mass transfer and intracrystalline plastic deformation,which can then be associated with the macrostructural geomechanical behavior.Compared to other types of rock,rock salt exhibits creep at lower temperatures.This behavior can be divided into three phases based on the changes in strain with time.However,at very low effective confining pressure and high deviatoric stress,rock salt can exhibit dilatant behavior,where brittle deformation could compromise the safety of underground gas storage in rock salt caverns.The proposed review presents the impact of purity,geochemistry and water content of rock salt on its geomechanical behavior,and thus,on the safety of the caverns. 展开更多
关键词 CREEP hydrogen IMPURITIES rock salt salt solution mining underground gas storage
下载PDF
益母草碱调节RhoA/ROCK信号通路对冠心病大鼠主动脉内皮细胞损伤的影响
16
作者 郭艳红 谢月敏 +2 位作者 冯强 郭少英 王静 《河北医学》 CAS 2024年第8期1272-1279,共8页
目的:研究益母草碱(Leo)通过调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对冠心病(CHD)大鼠主动脉内皮细胞损伤的影响及机制。方法:将造模成功的75只CHD大鼠随机分为模型组(CHD组)、Leo低、中、高剂量组(Leo-... 目的:研究益母草碱(Leo)通过调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对冠心病(CHD)大鼠主动脉内皮细胞损伤的影响及机制。方法:将造模成功的75只CHD大鼠随机分为模型组(CHD组)、Leo低、中、高剂量组(Leo-L组、Leo-M组、Leo-H组)和Leo高剂量组+RhoA激活剂LPA组(Leo-H+LPA组),每组15只。Leo-L、Leo-M、Leo-H组分别灌胃15、30、60mg·kg^(-1)·d^(-1)的Leo,Leo-H+LPA组灌胃60mg·kg^(-1)·d^(-1)的Leo,另需腹腔注射1mg·kg^(-1)·d^(-1)的LPA,Control组和CHD组以相同方式给予等量生理盐水,均连续干预4周。超声心动图测定心功能指标;全自动生化分析仪测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平;酶联免疫吸附测定(ELISA)检测白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、一氧化氮(NO)、内皮素1(ET-1)、内皮细胞特异性分子-1(EMS1)、血管内皮细胞粘附分子1(VCAM-1)水平;苏木精-伊红(HE)染色观察主动脉内皮损伤;流式细胞仪检测主动脉内皮细胞凋亡;免疫印迹法(Western blot)检测RhoA/ROCK信号通路相关蛋白表达。结果:与Control组相比,CHD组主动脉壁增厚且染色不均匀,细胞膨大,排列紊乱,内皮粗糙;左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)、TC、TG、LDL-C、IL-6、TNF-α、ET-1、VCAM-1和EMS1水平、细胞凋亡率及RhoA、ROCK1和ROCK2蛋白表达显著升高(P<0.05),左室射血分数(LVEF)、缩短分数(FS)、HDL-C、NO水平显著降低(P<0.05)。与CHD组相比,Leo-L组、Leo-M组、Leo-H组主动脉染色均匀,动脉壁结构清晰,细胞排列紧密且形态较正常,内膜较平滑;LVEDD、LVESD、TC、TG、LDL-C、IL-6、TNF-α、ET-1、VCAM-1和EMS1水平、细胞凋亡率及RhoA、ROCK1和ROCK2蛋白表达逐渐降低(P<0.05),LVEF、FS、HDL-C、NO水平逐渐升高(P<0.05)。Leo-H+LPA组逆转了Leo对CHD大鼠的保护作用(P<0.05)。结论:Leo可能通过抑制RhoA/ROCK信号通路改善CHD大鼠心功能,降低血脂,抑制炎症,进而减轻CHD大鼠主动脉内皮细胞损伤。 展开更多
关键词 益母草碱 RhoA/rock信号通路 冠心病 内皮细胞损伤 血脂 炎症反应
下载PDF
Impact of effective stress on permeability for carbonate fractured-vuggy rocks 被引量:1
17
作者 Ke Sun Huiqing Liu +5 位作者 Juliana Y.Leung Jing Wang Yabin Feng Renjie Liu Zhijiang Kang Yun Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第3期942-960,共19页
To gain insight into the flow mechanisms and stress sensitivity for fractured-vuggy reservoirs,several core models with different structural characteristics were designed and fabricated to investigate the impact of ef... To gain insight into the flow mechanisms and stress sensitivity for fractured-vuggy reservoirs,several core models with different structural characteristics were designed and fabricated to investigate the impact of effective stress on permeability for carbonate fractured-vuggy rocks(CFVR).It shows that the permeability performance curves under different pore and confining pressures(i.e.altered stress conditions)for the fractured core models and the vuggy core models have similar change patterns.The ranges of permeability variation are significantly wider at high pore pressures,indicating that permeability reduction is the most significant during the early stage of development for fractured-vuggy reservoirs.Since each obtained effective stress coefficient for permeability(ESCP)varies with the changes in confining pressure and pore pressure,the effective stresses for permeability of four representative CFVR show obvious nonlinear characteristics,and the variation ranges of ESCP are all between 0 and 1.Meanwhile,a comprehensive ESCP mathematical model considering triple media,including matrix pores,fractures,and dissolved vugs,was proposed.It is proved theoretically that the ESCP of CFVR generally varies between 0 and 1.Additionally,the regression results showed that the power model ranked highest among the four empirical models mainly applied in stress sensitivity characterization,followed by the logarithmic model,exponential model,and binomial model.The concept of“permeability decline rate”was introduced to better evaluate the stress sensitivity performance for CFVR,in which the one-fracture rock is the strongest,followed by the fracture-vug rock and two-horizontalfracture rock;the through-hole rock is the weakest.In general,this study provides a theoretical basis to guide the design of development and adjustment programs for carbonate fractured-vuggy reservoirs. 展开更多
关键词 Effective stress PERMEABILITY Carbonate fractured-vuggy rocks structure characteristics Stress sensitivity
下载PDF
抑制Rho/ROCK通路对气道平滑肌细胞增殖与迁移的影响及相关机制
18
作者 崔云斐 卢清华 +3 位作者 黄晓 林炜南 黄婷 杨琴 《中国当代儿科杂志》 CAS CSCD 北大核心 2024年第9期974-981,共8页
目的研究抑制Ras同源基因(Ras homolog gene,Rho)/Rho相关卷曲螺旋形成蛋白激酶(Rhoassociated coiled-coil forming protein kinase,ROCK)通路对心肌素(myocardin,MYOCD)参与的气道平滑肌细胞增殖与迁移的影响及分子机制。方法体外构... 目的研究抑制Ras同源基因(Ras homolog gene,Rho)/Rho相关卷曲螺旋形成蛋白激酶(Rhoassociated coiled-coil forming protein kinase,ROCK)通路对心肌素(myocardin,MYOCD)参与的气道平滑肌细胞增殖与迁移的影响及分子机制。方法体外构建腺病毒载体Ad-ZsGreen-shRNA-hROCK1感染人气道平滑肌细胞,将细胞随机分为4组:ROCK1基因沉默对照(shNC)组、shNC+花生四烯酸(arachidonic acid,AA;Rho/ROCK通路激活剂)组、ROCK1基因沉默(shROCK1)组、shROCK1+AA组(各组n=3)。采用实时荧光定量聚合酶链反应法、Western blot法检测ROCK1、MYOCD mRNA和蛋白表达水平,ELISA法检测球状肌动蛋白和丝状肌动蛋白水平,免疫荧光染色法及细胞划痕实验检测细胞增殖及迁移情况。结果与shNC+AA组相比,shROCK1+AA组ROCK1、MYOCD mRNA和蛋白表达水平降低,球状肌动蛋白、丝状肌动蛋白表达降低,细胞增殖、细胞迁移能力降低(P<0.05)。结论抑制Rho/ROCK通路致气道平滑肌细胞增殖及迁移能力受抑,其机制可能与MYOCD的下调有关. 展开更多
关键词 哮喘 Rho/rock通路 心肌素 增殖 气道平滑肌细胞
下载PDF
Drilling-based measuring method for the c-φ parameter of rock and its field application 被引量:3
19
作者 Bei Jiang Fenglin Ma +5 位作者 Qi Wang Hongke Gao Dahu Zhai Yusong Deng Chuanjie Xu Liangdi Yao 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第1期65-76,共12页
The technology of drilling tests makes it possible to obtain the strength parameter of rock accurately in situ. In this paper, a new rock cutting analysis model that considers the influence of the rock crushing zone(R... The technology of drilling tests makes it possible to obtain the strength parameter of rock accurately in situ. In this paper, a new rock cutting analysis model that considers the influence of the rock crushing zone(RCZ) is built. The formula for an ultimate cutting force is established based on the limit equilibrium principle. The relationship between digital drilling parameters(DDP) and the c-φ parameter(DDP-cφ formula, where c refers to the cohesion and φ refers to the internal friction angle) is derived, and the response of drilling parameters and cutting ratio to the strength parameters is analyzed. The drillingbased measuring method for the c-φ parameter of rock is constructed. The laboratory verification test is then completed, and the difference in results between the drilling test and the compression test is less than 6%. On this basis, in-situ rock drilling tests in a traffic tunnel and a coal mine roadway are carried out, and the strength parameters of the surrounding rock are effectively tested. The average difference ratio of the results is less than 11%, which verifies the effectiveness of the proposed method for obtaining the strength parameters based on digital drilling. This study provides methodological support for field testing of rock strength parameters. 展开更多
关键词 Digital drilling rock crushing zone c-u parameter Measurement method Field application
下载PDF
Rho/ROCK通路与心律失常的研究进展
20
作者 余意 杨波 《心血管病学进展》 CAS 2024年第7期577-580,共4页
越来越多的证据表明,Rho/ROCK通路在不同程度上调控着细胞的生长、发育、迁移、增殖和死亡,并在各种疾病中发挥重要作用。此外,Rho/ROCK通路与成纤维细胞、Ca^( 2+)和炎症因子等的相互作用对肺动脉高压和心血管疾病的发生与发展产生了... 越来越多的证据表明,Rho/ROCK通路在不同程度上调控着细胞的生长、发育、迁移、增殖和死亡,并在各种疾病中发挥重要作用。此外,Rho/ROCK通路与成纤维细胞、Ca^( 2+)和炎症因子等的相互作用对肺动脉高压和心血管疾病的发生与发展产生了重要的影响。近年来Rho/ROCK通路在心律失常中发挥的作用备受关注,现通过总结相关研究,为抗心律失常药的研究提供进一步参考。 展开更多
关键词 Rho/rock通路 心律失常 电生理
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部