[Objective] This study aimed to investigate the elimination regularity of ronidazole residues in chickens. [Method] Normal, healthy, 85-day-old Jinling broilers were selected as experimental chickens and fed with comp...[Objective] This study aimed to investigate the elimination regularity of ronidazole residues in chickens. [Method] Normal, healthy, 85-day-old Jinling broilers were selected as experimental chickens and fed with complete feed containing different concentrations of ronidazole. Ronidazole residues in chicken feather and edible tissues were detected by high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS） method to compare the elimination regularity. [Result] Data analysis showed that the amount of ronidazole residues in chicken feather and edible tissues was positively correlated with the concentration of ronidazole administered. After drug withdrawal, there was a significant correlation between the elimination of ronidazole residues in chicken feather and edible tissues. Thus, the prediction model of ronidazole residues in chicken feather could be established. [Conclusion] This study could improve in vivo detection system of ronidazole residues and provide reference for monitoring and controlling drug residues in livestock and poultry oroduction.展开更多
The Metronidazole(MTZ)/nitroreductase(NTR)-mediated cell ablation system is the most commonly used chemical-genetic cell ablation method in zebrafish. This system can specifically ablate target cells under spatial and...The Metronidazole(MTZ)/nitroreductase(NTR)-mediated cell ablation system is the most commonly used chemical-genetic cell ablation method in zebrafish. This system can specifically ablate target cells under spatial and temporal control. The MTZ/NTR system has become a widely used cell ablation system in biological, developmental, and functional studies. However, the inadequate cell-ablation ability of some cell types and the side effects of high concentration MTZ impede extensive applications of the MTZ/NTR system. In the present study, the US drug collection library was searched to extend the NTR system. Six MTZ analogs were found, and the cell-ablation ability of these analogs was tested in zebrafish larvae. The results revealed that two of the NTR substrates, Furazolidone and Ronidazole, ablated target cells more efficiently than MTZ at lower concentrations. Furthermore, the working concentration of Ronidazole, but not Furazolidone and MTZ, did not affect axonal bridge formation during spinal cord regeneration. Our results,taken together, indicate that Ronidazole is a superior prodrug to MTZ for the NTR system, especially for the study of neuron regeneration in zebrafish larvae.展开更多
文摘[Objective] This study aimed to investigate the elimination regularity of ronidazole residues in chickens. [Method] Normal, healthy, 85-day-old Jinling broilers were selected as experimental chickens and fed with complete feed containing different concentrations of ronidazole. Ronidazole residues in chicken feather and edible tissues were detected by high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS） method to compare the elimination regularity. [Result] Data analysis showed that the amount of ronidazole residues in chicken feather and edible tissues was positively correlated with the concentration of ronidazole administered. After drug withdrawal, there was a significant correlation between the elimination of ronidazole residues in chicken feather and edible tissues. Thus, the prediction model of ronidazole residues in chicken feather could be established. [Conclusion] This study could improve in vivo detection system of ronidazole residues and provide reference for monitoring and controlling drug residues in livestock and poultry oroduction.
基金supported by the National Natural Science Foundation of China(31771594,31970763)National Natural Science Foundation of China(NSFC)/Research Grants Council(RGC)Joint Research Scheme(31961160726)Guangdong Science and Technology Plan projects(2019A030317001)。
文摘The Metronidazole(MTZ)/nitroreductase(NTR)-mediated cell ablation system is the most commonly used chemical-genetic cell ablation method in zebrafish. This system can specifically ablate target cells under spatial and temporal control. The MTZ/NTR system has become a widely used cell ablation system in biological, developmental, and functional studies. However, the inadequate cell-ablation ability of some cell types and the side effects of high concentration MTZ impede extensive applications of the MTZ/NTR system. In the present study, the US drug collection library was searched to extend the NTR system. Six MTZ analogs were found, and the cell-ablation ability of these analogs was tested in zebrafish larvae. The results revealed that two of the NTR substrates, Furazolidone and Ronidazole, ablated target cells more efficiently than MTZ at lower concentrations. Furthermore, the working concentration of Ronidazole, but not Furazolidone and MTZ, did not affect axonal bridge formation during spinal cord regeneration. Our results,taken together, indicate that Ronidazole is a superior prodrug to MTZ for the NTR system, especially for the study of neuron regeneration in zebrafish larvae.
