Panax ginseng C.A.Mey.is an important plant species used in traditional Chinese medicine,whose primary active ingredient is a ginsenoside.Ginsenoside biosynthesis is not only regulated by transcription factors but als...Panax ginseng C.A.Mey.is an important plant species used in traditional Chinese medicine,whose primary active ingredient is a ginsenoside.Ginsenoside biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural genes.Nonetheless,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary metabolites.Squalene epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as terpenoid.Using ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in ginseng.We first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside biosynthesis.Next,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root clones.Thereafter,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,respectively.Then,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside biosynthesis.Thus,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.展开更多
Celts, a name full of mysteries, represent the unique cultural origin for the whole western countries, including those in Europe and America. It ever experienced its infancy, its prime, and also its decline, even near...Celts, a name full of mysteries, represent the unique cultural origin for the whole western countries, including those in Europe and America. It ever experienced its infancy, its prime, and also its decline, even nearly on the verge of extinction. Later, in the late 19 th and 20 th centuries, a variety of movements and trends occurring in western countries saw a renewed interest in aspects of Celtic culture, in pursuit of the unique cultural charm of Celts.展开更多
[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistr...[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a ＂U＂-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a ＂U＂ shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.展开更多
Estrogen affects the generation and transmission of neuropathic pain,but the specific regulatory mechanism is still unclear.Activation of the N-methyl-D-aspartate acid receptor 1（NMDAR1） plays an important role in t...Estrogen affects the generation and transmission of neuropathic pain,but the specific regulatory mechanism is still unclear.Activation of the N-methyl-D-aspartate acid receptor 1（NMDAR1） plays an important role in the production and maintenance of hyperalgesia and allodynia.The present study was conducted to determine whether a relationship exists between estrogen and NMDAR1 in peripheral nerve pain.A chronic sciatic nerve constriction injury model of chronic neuropathic pain was established in rats.These rats were then subcutaneously injected with 17β-estradiol,the NMDAR1 antagonist D（-）-2-amino-5-phosphonopentanoic acid（AP-5）,or both once daily for 15 days.Compared with injured drug na？ve rats,rats with chronic sciatic nerve injury that were administered estradiol showed a lower paw withdrawal mechanical threshold and a shorter paw withdrawal thermal latency,indicating increased sensitivity to mechanical and thermal pain.Estrogen administration was also associated with increased expression of NMDAR1 immunoreactivity（as assessed by immunohistochemistry） and protein（as determined by western blot assay） in spinal dorsal root ganglia.This 17β-estradiol-induced increase in NMDAR1 expression was blocked by co-administration with AP-5,whereas AP-5 alone did not affect NMDAR1 expression.These results suggest that 17β-estradiol administration significantly reduced mechanical and thermal pain thresholds in rats with chronic constriction of the sciatic nerve,and that the mechanism for this increased sensitivity may be related to the upregulation of NMDAR1 expression in dorsal root ganglia.展开更多
In order to provide theoretical basis and technical support for the afforestation and artificial water supply of P.szechuanica in arid areas,the characteristics of water consumption of P.szechuanica were explored,and ...In order to provide theoretical basis and technical support for the afforestation and artificial water supply of P.szechuanica in arid areas,the characteristics of water consumption of P.szechuanica were explored,and the law of water demand of P.szechuanica was grasped.In this paper,potted seedlings of 1-0 rooted cuttings of P.szechuanica were taken as research objects,and change situation of water consumption under different water control gradients was measured regularly by using weighing method,further analyzing dynamic change of water consumption of P.szechuanica and revealing water demand law of 1-0 rooted cuttings of P.szechuanica.The results showed that total change of water consumption of 1-0 rooted cuttings of P.szechuanica had"slow-fast-slow-fast"double-peak trend in the growth period of the current year,and corresponded with univariate linear relation(R^(2)=0.7137),with significant difference.In whole growth period,water consumption in August was the highest,which was 2.7 times of that in June and July and 1.5 times of that after September.In different water control treatments,the dynamic changes of daily and monthly water consumption were significantly different.In seven water control treatments,monthly water consumption was between(6315.95±1690.70)and(10105.28±3065.30)g/month,and mean was(8211.07±2308.23)g/month.With intensification of water control treatment,water consumption increased,but there was no seedling death due to water shortage.P.szechuanica has great plasticity in water demand,and can survive in both arid and humid environments.Meanwhile,it is revealed that P.szechuanica is the most widely distributed tree species in the region.展开更多
Lateral root is primary organ for plant to explore and utilize soil nutrient efficiently. The development of lateral roots (LR) is controlled by both genetic factors and nutrient status in environment. To investigate ...Lateral root is primary organ for plant to explore and utilize soil nutrient efficiently. The development of lateral roots (LR) is controlled by both genetic factors and nutrient status in environment. To investigate the effects of nitrate (NO3-) on rice lateral root growth and nitrogen (N) uptake efficiency under upland condition, three treatments, including root-split culture and whole plant culture in N sufficient and deficient conditions, were used in a vermiculite culture experiment. Root-split treatment showed that the growth of lateral roots was stimulated by localized nitrate supply. However, in whole plant culture, elongation of lateral roots was induced by NO3- deficiency. The effects of NO3- on rice lateral root growth were genotype-dependent. Similar N concentration, soluble sugar concentration and N content in shoot were observed in both root-split treatment and whole plant culture under NO3- sufficient condition, suggesting that the nitrogen requirement for rice normal growth could be satisfied with only half of roots supplied with NO3-. In the root-split treatment, N uptake was positively correlated with the average of lateral root length (ALRL) in NO3--supplied side, suggesting that the ALRL is important for rice root N uptake in the environment where the nitrogen nutrient is limiting factor. No significant correlation was observed between N uptake and ALRL in whole plant culture under N sufficient condition, which implies that the length of lateral roots may not be the main factor to determine tire rice root N uptake in nutrient-rich zone. Morphological and metabolic evidence in this study provided some prospects for genetic improvement of root system characters to improve the efficiency of nutrient absorption in rice.展开更多
With the development of economy and society, our country is on the road of rule by law, the legal system is progressing constantly. At the same time, we should also see that the administration according to law is not ...With the development of economy and society, our country is on the road of rule by law, the legal system is progressing constantly. At the same time, we should also see that the administration according to law is not perfect, and its shortcomings exist in different degree and need to be further improved. Based on the analysis of the status quo of grass-roots administrative law enforcement in China, this paper tries to put forward corresponding countermeasures, so as to promote the work of law-based administration for the corresponding study.展开更多
基金This work was supported by an award from the Department of Science and Technology of Jilin Province(20210402043GH and 20210204063YY).
文摘Panax ginseng C.A.Mey.is an important plant species used in traditional Chinese medicine,whose primary active ingredient is a ginsenoside.Ginsenoside biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural genes.Nonetheless,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary metabolites.Squalene epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as terpenoid.Using ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in ginseng.We first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside biosynthesis.Next,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root clones.Thereafter,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,respectively.Then,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside biosynthesis.Thus,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.
文摘Celts, a name full of mysteries, represent the unique cultural origin for the whole western countries, including those in Europe and America. It ever experienced its infancy, its prime, and also its decline, even nearly on the verge of extinction. Later, in the late 19 th and 20 th centuries, a variety of movements and trends occurring in western countries saw a renewed interest in aspects of Celtic culture, in pursuit of the unique cultural charm of Celts.
基金Supported by Natural Science Foundation of Ningxia Hui Autonomous Region(NZ0932)National Key Technology R & D Program of China(20068AI06A15-11)~~
文摘[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a ＂U＂-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a ＂U＂ shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.
基金supported by the Youth Shihezi University Applied Basic Research Project of China,No.2015ZRKYQ-LH19
文摘Estrogen affects the generation and transmission of neuropathic pain,but the specific regulatory mechanism is still unclear.Activation of the N-methyl-D-aspartate acid receptor 1（NMDAR1） plays an important role in the production and maintenance of hyperalgesia and allodynia.The present study was conducted to determine whether a relationship exists between estrogen and NMDAR1 in peripheral nerve pain.A chronic sciatic nerve constriction injury model of chronic neuropathic pain was established in rats.These rats were then subcutaneously injected with 17β-estradiol,the NMDAR1 antagonist D（-）-2-amino-5-phosphonopentanoic acid（AP-5）,or both once daily for 15 days.Compared with injured drug na？ve rats,rats with chronic sciatic nerve injury that were administered estradiol showed a lower paw withdrawal mechanical threshold and a shorter paw withdrawal thermal latency,indicating increased sensitivity to mechanical and thermal pain.Estrogen administration was also associated with increased expression of NMDAR1 immunoreactivity（as assessed by immunohistochemistry） and protein（as determined by western blot assay） in spinal dorsal root ganglia.This 17β-estradiol-induced increase in NMDAR1 expression was blocked by co-administration with AP-5,whereas AP-5 alone did not affect NMDAR1 expression.These results suggest that 17β-estradiol administration significantly reduced mechanical and thermal pain thresholds in rats with chronic constriction of the sciatic nerve,and that the mechanism for this increased sensitivity may be related to the upregulation of NMDAR1 expression in dorsal root ganglia.
基金Supported by Natural Science Foundation of Tibet Autonomous Region(XZ2019ZRG-58)Forestry Discipline Innovation Team Construction Project of Tibet Agriculture&Animal Husbandry University(Tibet Financial Forecast:2020-001).
文摘In order to provide theoretical basis and technical support for the afforestation and artificial water supply of P.szechuanica in arid areas,the characteristics of water consumption of P.szechuanica were explored,and the law of water demand of P.szechuanica was grasped.In this paper,potted seedlings of 1-0 rooted cuttings of P.szechuanica were taken as research objects,and change situation of water consumption under different water control gradients was measured regularly by using weighing method,further analyzing dynamic change of water consumption of P.szechuanica and revealing water demand law of 1-0 rooted cuttings of P.szechuanica.The results showed that total change of water consumption of 1-0 rooted cuttings of P.szechuanica had"slow-fast-slow-fast"double-peak trend in the growth period of the current year,and corresponded with univariate linear relation(R^(2)=0.7137),with significant difference.In whole growth period,water consumption in August was the highest,which was 2.7 times of that in June and July and 1.5 times of that after September.In different water control treatments,the dynamic changes of daily and monthly water consumption were significantly different.In seven water control treatments,monthly water consumption was between(6315.95±1690.70)and(10105.28±3065.30)g/month,and mean was(8211.07±2308.23)g/month.With intensification of water control treatment,water consumption increased,but there was no seedling death due to water shortage.P.szechuanica has great plasticity in water demand,and can survive in both arid and humid environments.Meanwhile,it is revealed that P.szechuanica is the most widely distributed tree species in the region.
文摘Lateral root is primary organ for plant to explore and utilize soil nutrient efficiently. The development of lateral roots (LR) is controlled by both genetic factors and nutrient status in environment. To investigate the effects of nitrate (NO3-) on rice lateral root growth and nitrogen (N) uptake efficiency under upland condition, three treatments, including root-split culture and whole plant culture in N sufficient and deficient conditions, were used in a vermiculite culture experiment. Root-split treatment showed that the growth of lateral roots was stimulated by localized nitrate supply. However, in whole plant culture, elongation of lateral roots was induced by NO3- deficiency. The effects of NO3- on rice lateral root growth were genotype-dependent. Similar N concentration, soluble sugar concentration and N content in shoot were observed in both root-split treatment and whole plant culture under NO3- sufficient condition, suggesting that the nitrogen requirement for rice normal growth could be satisfied with only half of roots supplied with NO3-. In the root-split treatment, N uptake was positively correlated with the average of lateral root length (ALRL) in NO3--supplied side, suggesting that the ALRL is important for rice root N uptake in the environment where the nitrogen nutrient is limiting factor. No significant correlation was observed between N uptake and ALRL in whole plant culture under N sufficient condition, which implies that the length of lateral roots may not be the main factor to determine tire rice root N uptake in nutrient-rich zone. Morphological and metabolic evidence in this study provided some prospects for genetic improvement of root system characters to improve the efficiency of nutrient absorption in rice.
文摘With the development of economy and society, our country is on the road of rule by law, the legal system is progressing constantly. At the same time, we should also see that the administration according to law is not perfect, and its shortcomings exist in different degree and need to be further improved. Based on the analysis of the status quo of grass-roots administrative law enforcement in China, this paper tries to put forward corresponding countermeasures, so as to promote the work of law-based administration for the corresponding study.
