Sugar beet root maggot （SBRM, Tetanops myopaeformis von R6der） is a major but poorly understood insect pest of sugar beet （Beta vulgaris L.）. The molecular mecha- nisms underlying plant defense responses are well ...Sugar beet root maggot （SBRM, Tetanops myopaeformis von R6der） is a major but poorly understood insect pest of sugar beet （Beta vulgaris L.）. The molecular mecha- nisms underlying plant defense responses are well documented, however, little information is available about complementary mechanisms for insect adaptive responses to overcome host resistance. To date, no studies have been published on SBRM gene expression pro- filing. Suppressive subtractive hybridization （SSH） generated more than 300 SBRM ESTs differentially expressed in the interaction of the pest with a moderately resistant （F1016） and a susceptible （F1010） sugar beet line. Blast2GO v. 3.2 search indicated that over 40% of the differentially expressed genes had known functions, primarily driven by fruit fly D. melanogaster genes. Expression patterns of 18 selected EST clones were confirmed by RT-PCR analysis. Gene Ontology （GO） analysis predicted a dominance of metabolic and catalytic genes involved in the interaction of SBRM with its host. SBRM genes function- ing during development, regulation, cellular process, signaling and under stress conditions were annotated. SBRM genes that were common or unique in response to resistant or susceptible interactions with the host were identified and their possible roles in insect responses to the host are discussed.展开更多
为明确辽宁地区韭菜田根蛆发生种类及优势种的消长动态,于2014-2017年分别采用黄色水盆诱集和挖根的方法对根蛆成虫和幼虫的发生和为害规律进行了系统调查研究。结果表明,辽宁韭菜田根蛆主要有韭菜迟眼蕈蚊Bradysia odoriphaga Yang et ...为明确辽宁地区韭菜田根蛆发生种类及优势种的消长动态,于2014-2017年分别采用黄色水盆诱集和挖根的方法对根蛆成虫和幼虫的发生和为害规律进行了系统调查研究。结果表明,辽宁韭菜田根蛆主要有韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang和食用菌异迟眼蕈蚊Bradysia difformis Frey,二者常常混合发生。北镇地区优势种为韭菜迟眼蕈蚊,年发生7~8代,其中冬季温室期发生3~4代。初冬扣棚后根蛆为害期与冬韭生产期高度重合。沈阳东陵地区优势种为食用菌异迟眼蕈蚊,其在露地韭菜上年发生5代。根蛆在田间呈聚集分布,聚集程度随种群密度的增大而升高。施用有机肥可显著降低根蛆的为害程度。根蛆以3龄幼虫在大葱上越冬。辽宁地区根蛆的发生规律呈现冬季发生重,春、秋季略轻发生,夏季基本不发生的特点。展开更多
针对我国根蛆类害虫严重危害葱蒜类蔬菜作物，特别在韭菜上问题突出的现状，项目开展了根蛆的生物学和生态学、种群规律、单项防治技术及综合防治技术的研究与示范工作。明确了我国大部分地区韭菜上的根蛆主要是韭菜迟眼蕈蚊Bradysiaodo...针对我国根蛆类害虫严重危害葱蒜类蔬菜作物，特别在韭菜上问题突出的现状，项目开展了根蛆的生物学和生态学、种群规律、单项防治技术及综合防治技术的研究与示范工作。明确了我国大部分地区韭菜上的根蛆主要是韭菜迟眼蕈蚊Bradysiaodoriphaga Yang et Zhang，在甘肃和辽宁地区的优势种为异迟眼蕈蚊B. difformis Frey。采用黄板诱集成虫结合挖根查幼虫的方法，明确了华北、东北、西北和华东地区根蛆在田间的发生规律。根蛆不耐高温耐低温，春、秋和冬季发生严重，在夏季有转主危害的趋势，冬季以老熟幼虫在0-5 cm土层中越冬。蕈蚊成虫对黑色和棕色趋性最强，研发出特异性的黑色粘虫板。覆盖50-60目防虫网对根蛆的防效最佳，且对韭菜生长有促进作用。建立了蕈蚊成虫的优势捕食性天敌——瘦弱秽蝇 Coenosia attenuata的饲养技术和田间应用技术，筛选出了对蕈蚊幼虫高致病力的昆虫病原线虫和Bt 菌株，田间防效达60%以上。研发出保护地灌溉臭氧水、减量施药和喷淋施药等轻简化的根蛆防治技术10项。根据不同地区韭菜的种植习惯和根蛆的发生特点，在辽宁、河北、山东、甘肃等地建立了区域性的根蛆类害虫综合防治技术，建立核心示范区10个，进行技术培训5000人次，取得了一定的经济、社会和生态效益。展开更多
文摘Sugar beet root maggot （SBRM, Tetanops myopaeformis von R6der） is a major but poorly understood insect pest of sugar beet （Beta vulgaris L.）. The molecular mecha- nisms underlying plant defense responses are well documented, however, little information is available about complementary mechanisms for insect adaptive responses to overcome host resistance. To date, no studies have been published on SBRM gene expression pro- filing. Suppressive subtractive hybridization （SSH） generated more than 300 SBRM ESTs differentially expressed in the interaction of the pest with a moderately resistant （F1016） and a susceptible （F1010） sugar beet line. Blast2GO v. 3.2 search indicated that over 40% of the differentially expressed genes had known functions, primarily driven by fruit fly D. melanogaster genes. Expression patterns of 18 selected EST clones were confirmed by RT-PCR analysis. Gene Ontology （GO） analysis predicted a dominance of metabolic and catalytic genes involved in the interaction of SBRM with its host. SBRM genes function- ing during development, regulation, cellular process, signaling and under stress conditions were annotated. SBRM genes that were common or unique in response to resistant or susceptible interactions with the host were identified and their possible roles in insect responses to the host are discussed.
文摘为明确辽宁地区韭菜田根蛆发生种类及优势种的消长动态,于2014-2017年分别采用黄色水盆诱集和挖根的方法对根蛆成虫和幼虫的发生和为害规律进行了系统调查研究。结果表明,辽宁韭菜田根蛆主要有韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang和食用菌异迟眼蕈蚊Bradysia difformis Frey,二者常常混合发生。北镇地区优势种为韭菜迟眼蕈蚊,年发生7~8代,其中冬季温室期发生3~4代。初冬扣棚后根蛆为害期与冬韭生产期高度重合。沈阳东陵地区优势种为食用菌异迟眼蕈蚊,其在露地韭菜上年发生5代。根蛆在田间呈聚集分布,聚集程度随种群密度的增大而升高。施用有机肥可显著降低根蛆的为害程度。根蛆以3龄幼虫在大葱上越冬。辽宁地区根蛆的发生规律呈现冬季发生重,春、秋季略轻发生,夏季基本不发生的特点。
文摘针对我国根蛆类害虫严重危害葱蒜类蔬菜作物，特别在韭菜上问题突出的现状，项目开展了根蛆的生物学和生态学、种群规律、单项防治技术及综合防治技术的研究与示范工作。明确了我国大部分地区韭菜上的根蛆主要是韭菜迟眼蕈蚊Bradysiaodoriphaga Yang et Zhang，在甘肃和辽宁地区的优势种为异迟眼蕈蚊B. difformis Frey。采用黄板诱集成虫结合挖根查幼虫的方法，明确了华北、东北、西北和华东地区根蛆在田间的发生规律。根蛆不耐高温耐低温，春、秋和冬季发生严重，在夏季有转主危害的趋势，冬季以老熟幼虫在0-5 cm土层中越冬。蕈蚊成虫对黑色和棕色趋性最强，研发出特异性的黑色粘虫板。覆盖50-60目防虫网对根蛆的防效最佳，且对韭菜生长有促进作用。建立了蕈蚊成虫的优势捕食性天敌——瘦弱秽蝇 Coenosia attenuata的饲养技术和田间应用技术，筛选出了对蕈蚊幼虫高致病力的昆虫病原线虫和Bt 菌株，田间防效达60%以上。研发出保护地灌溉臭氧水、减量施药和喷淋施药等轻简化的根蛆防治技术10项。根据不同地区韭菜的种植习惯和根蛆的发生特点，在辽宁、河北、山东、甘肃等地建立了区域性的根蛆类害虫综合防治技术，建立核心示范区10个，进行技术培训5000人次，取得了一定的经济、社会和生态效益。
