Nutrient Deficiency Affects Root Architecture of Young Seedlings of Malus hupehensis (Pamp) Rehd. Under Conditions of Artificial Medium Cultivation

What the researchers go in for is to establish models between root architecture （RA） changes and nutrition, mold ideal root architecture of apple trees, improve the nutrient uptake efficiency, and further explore the functional mechanism of nutrient elements during the course of RA construction. The cultivation system of filter paper is utilized to research the effect of nutrient deficiency on the RA of Malus hupehensis （Pamp.） Rehd. There may be eight types of RA. In complete Hogland solution, the main type of RA is ＂lateral roots clustering in the upper and middle regions of primary root＂. With the lack of P, K or Ca, the main type of RA is ＂lateral roots clustering in the upper region primary root＂, and the ＂lateral roots clustering in the upper and middle regions of primary root＂ types of RA decrease. But with shortage of P, the type of lateral roots clustering in the upper and lower regions of primary root increases, and the type of lateral roots clustering in the middle region of primary root decreases, with the types of RA diversified. Under the condition of K deficiency, the type of no lateral root increases and types of lateral roots clustering in the middle region of primary root decrease, and the percentage of such types as ＂no lateral root＂, ＂lateral roots clustering in the upper region of primary root＂, and ＂lateral roots clustering in the upper and middle regions of primary root＂ accounts for 97.9% in all, with the types of RA simplified. With lack of Fe, Mg or Zn, the main type of RA is ＂lateral roots clustering in the upper and middle regions of primary root＂, but the type of lateral roots evenly-distributed on primary root increases. The main type of RA is ＂lateral roots evenlydistributed on primary root＂, under the condition of N deficiency, and the types of RA turn out to be diversified. There exists a close relation between nutrient deficiency and RA changes. Owing to various forms of nutrient deficiency, correspondingly different types of RA have been produced.

Transcriptome analysis reveals the effects of alkali stress on root system architecture and endogenous hormones in apple rootstocks

Soil alkalinity is a major factor that restricts the growth of apple roots.To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia (alkali-tolerant) and Malus hupehensis (alkali-sensitive), were compared. To understand alkali tolerance of M. prunifolia at the molecular level, transcriptome analysis was performed. When plants were cultured in alkaline conditions for 15 d, the root growth of M. hupehensis with weak alkali tolerance decreased significantly. Analysis of endogenous hormone levels showed that the concentrations of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in M. hupehensis under alkali stress were lower than those in the control. However, the trend for IAA and ZR in M. prunifolia was the opposite. The concentration of abscisic acid (ABA) in the roots of the two apple rootstocks under alkali stress increased, but the concentration of ABA in the roots of M. prunifolia was higher than that in M. hupehensis. The expression of IAA-related genes ARF5, GH3.6, SAUR36, and SAUR32 and the Cytokinin (CTK)-related gene IPT5 in M. prunifolia was higher than those in the control, but the expression of these genes in M. hupehensis was lower than those in the control. The expression of ABA-related genes CIPK1 and AHK1 increased in the two apple rootstocks under alkali stress, but the expression of CIPK1 and AHK1 in M. prunifolia was higher than in M. hupehensis. These results demonstrated that under alkali stress, the increase of IAA, ZR, and ABA in roots and the increase of the expression of related genes promoted the growth of roots and improved the alkali tolerance of apple rootstocks.

Interacted Effect of Arbuscular Mycorrhizal Fungi and Polyamines on Root System Architecture of Citrus Seedlings

Either arbuscular mycorrhizal fungi （AMF） or polyamines （PAs） may change root system architecture （RSA） of plants, whereas the interaction of AMF and PAs on RSA remains unclear. In the present study, we studied the interaction between AMF （Paraglomus occultum） and exogenous PAs, including putrescine （Put）, spermidine （Spd） and spermine （Spin） on mycorrhizal development of different parts of root system, plant growth, RSA and carbohydrate concentrations of 6-m-old citrus （Citrus tangerine Hort. ex Tanaka） seedlings. After 14 wk of PAs application, PA-treated mycorrhizal seedlings exhibited better mycorrhizal colonization and numbers of vesicles, arbuscules, and entry points, and the best mycorrhizal status of taproot, first-, second-, and third-order lateral roots was respectively found in mycorrhizal seedlings supplied with Put, Spd and Spm, suggesting that PAs might act as a regulated factor of mycorrhizal development through transformation of root sucrose more into glucose for sustaining mycorrhizal development. AMF usually notably increases RSA traits （taproot length, total length, average diameter, projected area, surface area, volume, and number of first-, second-, and third-order lateral roots） of only PA-treated seedlings. Among the three PA species, greater positive effects on RSA change and plant biomass increment of the seedlings generally rank as Spd〉Spm〉Put, irrespective of whether or not AMF colonization. PAs significantly changed the RSA traits in mycorrhizal but not in non-mycorrhizal seedlings. It suggests that the application of PAs （especially Spd） to AMF plants would optimize RSA of citrus seedlings, thus increasing plant growth （shoot and root dry weight）.

Identification of QTL and underlying genes for root system architecture associated with nitrate nutrition in hexaploid wheat

The root system architecture(RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource adaptive responses in wheat(Triticum aestivum L.). Here, a high-throughput germination paper-based plant phenotyping system was used to identify seedling traits in a wheat doubled haploid mapping population, Savannah×Rialto. Significant genotypic and nitrate-N treatment variation was found across the population for seedling traits with distinct trait grouping for root size-related traits and root distribution-related traits. Quantitative trait locus(QTL) analysis identified a total of 59 seedling trait QTLs. Across two nitrate treatments, 27 root QTLs were specific to the nitrate treatment. Transcriptomic analyses for one of the QTLs on chromosome 2 D, which was found under low nitrate conditions, revealed gene enrichment in N-related biological processes and 28 differentially expressed genes with possible involvement in a root angle response. Together, these findings provide genetic insight into root system architecture and plant adaptive responses to nitrate, as well as targets that could help improve N capture in wheat.

Root architecture characteristics of plant inlay in live slope grating

In the experimental garden of the Department of Soil Bioengineering and Landscape Construction, University of Applied Life Sciences in Vienna, Austria, coarse root systems of three different brush species were completely excavated and semiutomatically digitized. The species were Lonicera xylosteum, Ligustrum vulgare and Euonymus europaeus. The 3-D root architectures reveal different growth strategies between species, which are related to ecological characteristics and physical soil properties. The root architecture of Lonicera xylosteum and Ligustrum vulgare, planted in the under layer of the live slope grading, where the soil is very tight and the soil water content and fertility are relatively low, is shallow. However, the root distribution of E. europaeus, planted in the middle layer, where environmental conditions are better, is deeper. Most of the root biomass of the three species is concentrated in the 0-30 cm soil layer. A quarter of the root biomass ofLigustrum vulgare is distributed in the upper layer of the plant inlay. E. europaeus has a relatively even distribution in the 30-0 cm and 60-90 cm soil layer.

Ecological adaptation of Reaumuria soongorica root system architecture to arid environments

The architectural parameters of Reaumuria soongorica root system in different habitats of Gansu Province, China were analyzed to examine its ecological adaptability to arid environments. Results show that： （1） Topological indices of R. Soongorica root sys- tem are small in all habitats, and root branching pattem tends to be dichotomous. Also, the indices gradually increase in the Min- qin windblown sand region and the Zhangye Gobi region in Hexi Corridor, which indicates that drought tends to produce her- ringbone-like root branching pattems. （2） Fractal dimension values ofR. Soongorica root system are small and not obvious in the Minqin windblown sand region and the Zhangye Gobi region in Hexi Corridor, with values of 1.1778 and 1.1169, respectively. Fractal dimension values are relatively large in Jiuzhoutai semi-arid hilly and gully region of the Loess Plateau, which indicates that the R. Soongorica root system has better fractal characteristics in this region than in the other regions. （3） Total branching ra- tios of the R. Soongorica root system in arid regions of Hexi Corridor are smaller than that in the Jiuzhoutai semi-arid hilly and gully region of the Loess Plateau. This shows that root branching ability in the semi-arid region is stronger, and it decreases to some degree with increased drought. （4） The root connection lengths of R. soongorica root system are long in all habitats, but there are significant length differences between the different habitats. The root connection length at the Minqin windblown sand region is the longest. It is concluded that R. soongoriea adapts to arid environments by decreasing root branching, decreasing root overlap and increasing root connection length, which makes its root branching pattern tend to be herringbone-like to reduce com- petition in root internal environment for nutrients and to enhance root absorption rate of nutrients, and ensure effective nutrition space. Thus the roots can absorb enough water and nutrients in resource-poor settings to ensure normal physiological requirements.

Development of Root Phenotyping Platforms for Identification of Root Architecture Mutations in EMS-Induced and Low-Path-Sequenced Sorghum Mutant Population

Sorghum’s natural adaptation to a wide range of abiotic stresses provides diverse genetic reserves for potential improvement in crop stress tolerance. Growing interest in sorghum research has led to the expansion of genetic resources though establishment of the sorghum association panel (SAP), generation of mutagenized populations, and recombinant inbred line (RIL) populations, etc. Despite rapid improvement in biotechnological tools, lack of efficient phenotyping platforms remains one of the major obstacles in utilizing these genetic resources. Scarcity of efforts in root system phenotyping hinders identification and integration of the superior root traits advantageous to stress tolerance. Here, we explored multiple approaches in root phenotyping of an ethyl methanesulfonate (EMS)-mutagenized sorghum population. Paper-based growth pouches (PGP) and hydroponics were employed to analyze root system architecture (RSA) variations induced by mutations and to test root development flexibility in response to phosphorus deficiency in early growing stages. PGP method had improved capabilities compared to hydroponics providing inexpensive, space-saving, and high-throughput phenotyping of sorghum roots. Preliminary observation revealed distinct phenotypic variations which were qualitatively and quantitatively systemized for association analysis. Phenotypes/ideotypes with root architecture variations potentially correlated with Pi acquisition were selected to evaluate their contribution to P-efficiency (PE). Sand mixed with P-loaded activated alumina substrate (SAS) provided closely to natural but still controlled single-variable conditions with regulated Pi availability. Due to higher labor and cost input we propose SAS to be used for evaluating selected sorghum candidates for PE. The ability of rapidly screening root phenotypes holds great potential for discovering genes responsible for relevant root traits and utilizing mutations to improve nutrient efficiency and crop productivity.

Hardware Architecture for RSA Cryptography Based on Residue Number System

A parallel architecture for efficient hardware implementation of Rivest Shamir Adleman(RSA) cryptography is proposed.Residue number system(RNS) is introduced to realize high parallelism,thus all the elements under the same base are independent of each other and can be computed in parallel.Moreover,a simple and fast base transformation is used to achieve RNS Montgomery modular multiplication algorithm,which facilitates hardware implementation.Based on transport triggered architecture(TTA),the proposed architecture is designed to evaluate the performance and feasibility of the algorithm.With these optimizations,a decryption rate of 106 kbps can be achieved for 1 024-b RSA at the frequency of 100 MHz.

Effect of Acid Treatment on Root Architecture in Seedlings of <i>Malus hupehensis</i>var. <i>pingyiensis</i>

The purpose of this paper is to explore the effects of acid treatment on root morphology and architecture in seedlings of Malus hupehensis var. pingyiensis. The rootstock seedlings were cultured in 1/2 Hoagland nutrient solutions of different pH (pH 4, pH 4.5, pH 5 and pH 6), respectively. The parameters of root architecture were measured in the day 4, 8 and 12 with the professional WinRHIZO 2007. Compared with the control (pH 6), the treatments significantly decreased the fractal dimension, length, diameter, surface area and volume of roots in day 8 and 12, and they kept decreasing followed the increase of the acidity and treatment time. The growth of lateral roots was more susceptible to acid treatment than taproots. In addition, the acid treatment mainly inhibited the growth of rootlets, significantly decreased the proportion of rootlets that changed the composition of roots, and then simplified the space structure of roots.

The influence of plant root system architectural properties upon the stability of loess hillslopes,Northeast Qinghai,China

To investigate the influence of root system architectural properties of three indigenous （cold- adapted） shrubs on the hillslope stability of loess deposits in the Xining Basin, northeast part of Qinghai-Tibet Plateau （QTP）, indoor direct shear tests have been conducted on the remolded rooted soil of three shrubs. Test results show that root system architectural indices （root area ratio （RAR）, root length density （RLD） and root density （RD）） of the shrubs decline with depth and the relationship between RAR, RD and depth is exponential, while a power relationship describes the relationship between RLD and depth. The cohesion force of remolded rooted soil for the shrubs initially increases with depth, but it then demonstrates a slightly decreasing trend, which can be described with a power relationship. Power relationships also describe relationships between cohesion force and RAR, RLD and RD for the shrubs. As the growth period increases from lO to 17 months, the incremental increase in RAR is 48.32% ~ 21o.25% for Caragana korshinskii Kom and 0.56% ~ 166.85% for ZygophyUum xanthoxylon （Bunge） Maxim. This proportional increase is notably larger than that for RLD and RD. The increment in RAR is marginally greater for C. korshinskff than it is for Z. xanthoxylon. Correspondingly, the cohesion force incremental rates of remolded rooted soil for C. korshinskii and Z. xanthoxylon are 12.41% ~ 25.22% and 3.45% ~ 17.33% respectively. Meanwhile, as root content increases, the contribution by roots to cohesion force increases markedly until a threshold condition is reached.

Increasing root-lower characteristics improves drought tolerance in cotton cultivars at the seedling stage

Drought is an important abiotic stress factor in cotton production.The root system architecture(RSA)of cotton shows high plasticity which can alleviate drought-related stress under drought stress(DS)conditions;however,this alleviation is cultivar dependent.Therefore,this study estimated the genetic variability of RSA in cotton under DS.Using the paper-based growth system,we assessed the RSA variability in 80 cotton cultivars at the seedling stage,with 0 and10%polyethylene glycol 6000(PEG6000)as the control(CK)and DS treatment,respectively.An analysis of 23 aboveground and root traits in the 80 cotton cultivars revealed different responses to DS.On the 10th day after DS treatment,the degree of variation in the RSA traits under DS(5–55%)was greater than that of CK(5–49%).The 80 cultivars were divided into drought-tolerant cultivars(group 1),intermediate drought-tolerant cultivars(group 2),and drought-sensitive cultivars(group 3)based on their comprehensive evaluation values of drought resistance.Under DS,the root lengthlower,root area-lower,root volume-lower,and root length density-lower were significantly reduced by 63,71,76,and 4%in the drought-sensitive cultivars compared to CK.Notably,the drought-tolerant cultivars maintained their root lengthlower,root area-lower,root volume-lower,and root length density–lower attributes.Compared to CK,the root diameter(0–2 mm)-lower increased by 21%in group 1 but decreased by 3 and 64%in groups 2 and 3,respectively,under DS.Additionally,the drought-tolerant cultivars displayed a plastic response under DS that was characterized by an increase in the root-lower characteristics.Drought resistance was positively correlated with the root area-lower and root length density-lower.Overall,the RSA of the different cotton cultivars varied greatly under DS.Therefore,important root traits,such as the root-lower traits,provide great insights for exploring whether drought-tolerant cotton cultivars can effectively withstand adverse environments.

RSA算法的CUDA高效实现技术

CUDA(Compute Unified Device Architecture)作为一种支持GPU通用计算的新型计算架构,在大规模数据并行计算方面得到了广泛的应用。RSA算法是一种计算密集型的公钥密码算法,给出了基于CUDA的RSA算法并行化高效实现技术,其关键为引入大量独立并发的Montgomery模乘线程,并给出了具体的线程组织、数据存储结构以及基于共享内存的性能优化实现技术。根据RSA算法CUDA实现方法,在某款GPU上测试了RSA算法的运算性能和吞吐率。实验结果表明,与RSA算法的通用CPU实现方式相比,CUDA实现能够实现超过40倍的性能加速。

基于RSA算法的数据加密卡的设计

针对电子商务活动中的信息安全问题,基于非对称公钥密码体制,研究了RSA算法的软、硬件实现,提出了一种用加密卡实现的数字签名方案.模拟运行表明,该方案能够快速实现数据加密且运行方便可靠.

使用改进的心动阵列结构实现RSA公共密钥算法

本文介绍了一种 RSA算法的电路实现结构。该结构是对心动阵列结构的改进 ,对心动阵列结构的核心模块长加法模块进行了循环复用 ,在大幅度降低电路面积的情况下 ,运算速度没有明显的降低。用 Verilog描述了整个设计 ,并在

对一类RSA变体的攻击

讨论RSA公钥密码体制在素因子p满足等式ex+by+c≡0(mod p)条件下的安全性,研究了对其格攻击的方法。利用方程小根求解问题对其进行攻击,攻击结果表明:当参数x,y满足|x||y|<N3β-3+(2β+1)1-β-ε时,通过格攻击方法可以有效的分解N,即满足这样条件的RSA公钥密码体制是不安全的。

改进的RSA密码算法的并行化实现

针对现有的RSA(Rivest-Shamir-Adelman)算法在云环境下运行速度慢的问题,提出了一种改进的RSA算法,该算法的基本思想是通过将RSA算法中部分解密端的计算量转移到加密端的方式,并且将统一计算设备架构(CUDA)的并行化实现技术应用于算法的解密端,在解密过程中大量引入并行的模乘线程来提高RSA算法模幂运算的速度,以达到加快解密的目的。实验结果表明,该算法相对于原RSA算法,解密速度可获得最大为10的加速比。

针对离散私钥比特泄漏的RSA格攻击方法

RSA算法是目前应用最广泛的公钥密码体制之一,而格攻击是针对RSA体制的一类重要攻击方法。为此,将RSA算法的部分私钥泄漏问题转化为多变元线性同余方程的求解问题,基于同余方程构造出特定的格,利用LLL格基约化算法进行约化,从而以一定的概率求得同余方程的小根。以上述多变元线性同余方程的小根求解技术为基础,提出一种针对离散私钥比特泄漏的RSA格攻击方法。在该方法下,如果RSA算法的公钥参数e=N~β≤N^(1/2),并且私钥d的未知部分N≤N^((1/2)-β),则能以高概率恢复出RSA算法的私钥d。通过NTL包对长度为1024 bit的大整数进行实验,结果验证了该攻击方法的有效性。

一款高吞吐率RSA密码处理器的设计(英文)

介绍了采用蒙哥马利模乘法算法和指数的从右到左的二进制方法,并根据大整数模乘法运算和VLSI实现的要求进行改进的RSA处理器,在提供高速RSA处理能力的同时,可抵抗某些定时分析攻击和功耗分析攻击。该RSA处理器在其模乘法器中使用了CSA(进位保留加法器)结构以避免长进位链,并采用一种新型(4∶2)压缩器结构以减少面积和延迟。提出了信号多重备份的方法,解决信号广播带来的大的负载和线长问题。数据通路的设计采用一种基于多选器的动态重构方法,其模乘法器可以执行一个1024位的模乘幂运算,也可以并行执行2个512位的模乘幂运算,从而支持基于中国剩余定理的加速策略。

RSA不动点的一个注记

给出RSA不动点数的一个证明,并给出RSA不动点的简单求解方法。

SET认证软件构架及RSA算法模型研究

以集成电子商务的CA认证软件开发为背景,探讨了SET认证安全问题的解决方案。首先提出SET认证的软件构架,阐述与层次模型相适应的智能软件构架设计思想;进而提出SET认证的协议模型,并分析其过程控制原理,重点探讨RSA安全算法的数学模型和多种建模机制的综合应用,给出基于主程序/子过程体系结构风格和形式描述的构件模型;最后给出RSA算法实现的ACME模板描述。所提出的构架分析过程和方法具有一般理论意义和工程实践价值。