Objective:To observe the effect of Rougan Huaqian granules combined with human mesenchymal stem cell(hMSC)transplantation on the liver fibrosis in carbon tetrachlorideinduced cirrhosis rats.Methods:Sixty SD rats were ...Objective:To observe the effect of Rougan Huaqian granules combined with human mesenchymal stem cell(hMSC)transplantation on the liver fibrosis in carbon tetrachlorideinduced cirrhosis rats.Methods:Sixty SD rats were randomly divided into live groups.The rats in control group received intraperitoneal injection of saline,while those in model control group,treatment group A,group B and group C received intraperitoneal injection of carbon tetrachloride oily solution to induce liver cirrhosis within 8 weeks.Then,the rats in the model control group,treatment group A,treatment group B,treatment group C received vein tail injection of saline,Rougan Huaqian granules,hMSC suspension and Rougan Huaqian granules combined with hMSC suspension.Results:The treatment groups had significantly different liver function(AST levels),liver fibrosis index(laminin and HA),hepatic sinusoidal wallsα-smooth muscle actin,Ⅳcollagen and laminin protein expression andⅠ,Ⅲcollagen from the model group(P<0.05).The transplanted cells showed human hepatocyte-like cells differentiation trend in the liver.Conclusions:The Rougan Huaqian granules combined with hMSC transplantation can alleviate liver fibrosis in cirrhosis rats.展开更多
Background:To explore the clinical efficacy of Huazhi Rougan granules in treating non-alcoholic fatty liver disease(NAFLD)and its effect on the oral and intestinal flora.Methods:48 patients who met the inclusion crite...Background:To explore the clinical efficacy of Huazhi Rougan granules in treating non-alcoholic fatty liver disease(NAFLD)and its effect on the oral and intestinal flora.Methods:48 patients who met the inclusion criteria were intervened with Huazhi Rougan granules,the liver and spleen CT ratio,Traditional Chinese medicine(TCM)syndrome,body mass index(BMI,WC,SMM,body fat percentage,visceral fat area),liver function,and blood lipid levels were compared before and after treatment,and the curative effect was evaluated;6 cases of NAFLD patients whose clinical efficacy after Huazhi Rougan granule intervention was effective were randomly selected,and the patient’s oral and intestinal samples were taken for further analysis.Results:After treatment,the total effective rate of clinical efficacy was 85.14%.The TCM symptoms of NAFLD patients were significantly alleviated compared with before treatment(P<0.05).There were statistically significant differences in ALT,AST,GGT,TC,and TG before and after treatment(P<0.01).After treatment,BW,BMI,WC,body fat percentage,visceral fat area,liver hardness,and fat attenuation index were significantly decreased(P<0.01).Oral-gut flora analysis showed:After treatment,the Shannon index of the gut microbial community increased(P<0.01).Compared with before treatment,the abundance of Firmicutes decreased,while the abundance of Bacteroidetes,Candidatus-Sa,and Tenericutes increased(P<0.05).At the genus level,the abundance of Bacteroides,Lactobacillus,and Bifidobacterium increased,and Streptococcus,Oribacterium,and Peptoanaerobacter decreased(P<0.05).Before and after treatment,the dominant bacterial phyla in the intestine were the same,including Bacteroidetes,Firmicutes,and Proteobacteria.The abundance of Firmicutes and Roseburia decreased after treatment(P<0.05).Conclusions:Huazhi Rougan granules have a significant effect on the treatment of NAFLD,can improve liver function,reduce blood lipids,can effectively reduce body morphological indicators such as body mass,WC,body fat percentage,visceral fat area,liver stiffness measurement(LSM),and fat content,and has the function of protecting the liver and reducing blood pressure,promoting the role of intrahepatic fat metabolism;and significantly relieve the clinical symptoms.NAFLD patients have the gut and oral flora disturbances.Huazhi Rougan granules can restore the diversity of intestinal microbial communities in NAFLD patients and play a therapeutic role by regulating the abundance and composition of oral and Gut flora.展开更多
[Objectives] To establish the preparation process and quality standard of Rougan Huaxian ointment.[Methods] The L 9(3 4) orthogonal test was employed to optimize the preparation process by considering the multiplicati...[Objectives] To establish the preparation process and quality standard of Rougan Huaxian ointment.[Methods] The L 9(3 4) orthogonal test was employed to optimize the preparation process by considering the multiplication of water addition, extraction time, and extraction frequency as influencing factors. The dry paste yield was utilized as the evaluation criterion, in conjunction with the actual production conditions. Thin layer chromatography was employed to identify Radix Astragali, Lycii Fructus, Herba Dendrobii, and Rhizoma Polygoni Cuspidati. Ethanol served as the solvent for the determination of ethanol-soluble extractives using the cold immersion method.[Results] The preparation process was conducted as follows: the specified quantity of medicinal materials was combined with water for extraction purposes, performed in two separate stages. In each stage, eight times the amount of water was added. The first extraction lasted for 1.5 h, while the second extraction was completed in 1.0 h. The resulting liquid was then concentrated into a thick paste with a relative density ranging from 1.25 to 1.30 at a temperature of 60 ℃. The thin-layer chromatography analysis of Radix Astragali, Lycii Fructus, Herba Dendrobii, and Rhizoma Polygoni Cuspidati demonstrated distinct spots, effective separation, and the absence of interference from negative samples. Additionally, the ethanol-soluble extractives yielded a minimum of 8.0% in terms of dry weight.[Conclusions] The preparation process for Rougan Huaxian ointment is both stable and feasible. Furthermore, the quality standards established for this preparation are unique and reproducible, thereby facilitating effective quality control.展开更多
Objective: Huazhi Rougan granules(HRGs) are a promising drug to improve alcoholic liver disease(ALD), but its mechanism remains unclear. Materials and Methods: Network pharmacology, molecular docking, and animal exper...Objective: Huazhi Rougan granules(HRGs) are a promising drug to improve alcoholic liver disease(ALD), but its mechanism remains unclear. Materials and Methods: Network pharmacology, molecular docking, and animal experiments were used to reveal the potential mechanism of HRG against ALD. Results: A total of 245 potential targets of HRG against ALD were obtained. Functional enrichment analysis suggests that HRG could synergistically regulate various biological pathways to exert therapeutic effects on ALD. Molecular docking showed that the key targets exhibited the good binding ability with the key compounds. The in vivo experiments showed that HRG can effectively alleviate pathological changes in liver tissue, improve blood lipid levels, antioxidant stress ability, and liver function, and reduce the release of inflammatory cytokines in the liver. Conclusions: This study reflects that HRG is an effective strategy for treating ALD, providing a basis for revealing the prevention and treatment mechanisms of ALD.展开更多
基金supported by the Guangxi Scientific and Technological Project(No.11107009-3-1)Guangxi Natural Science Fund Projects(No.2010 GXNSFA013211)
文摘Objective:To observe the effect of Rougan Huaqian granules combined with human mesenchymal stem cell(hMSC)transplantation on the liver fibrosis in carbon tetrachlorideinduced cirrhosis rats.Methods:Sixty SD rats were randomly divided into live groups.The rats in control group received intraperitoneal injection of saline,while those in model control group,treatment group A,group B and group C received intraperitoneal injection of carbon tetrachloride oily solution to induce liver cirrhosis within 8 weeks.Then,the rats in the model control group,treatment group A,treatment group B,treatment group C received vein tail injection of saline,Rougan Huaqian granules,hMSC suspension and Rougan Huaqian granules combined with hMSC suspension.Results:The treatment groups had significantly different liver function(AST levels),liver fibrosis index(laminin and HA),hepatic sinusoidal wallsα-smooth muscle actin,Ⅳcollagen and laminin protein expression andⅠ,Ⅲcollagen from the model group(P<0.05).The transplanted cells showed human hepatocyte-like cells differentiation trend in the liver.Conclusions:The Rougan Huaqian granules combined with hMSC transplantation can alleviate liver fibrosis in cirrhosis rats.
基金Project of the State Administration of Traditional Chinese Medicine:Key Laboratory of Liver and Kidney Treatment of Chronic Liver Diseases(No Z155080000004)Key project Natural Science Foundation of Hubei Province:(No.2020CFA023).
文摘Background:To explore the clinical efficacy of Huazhi Rougan granules in treating non-alcoholic fatty liver disease(NAFLD)and its effect on the oral and intestinal flora.Methods:48 patients who met the inclusion criteria were intervened with Huazhi Rougan granules,the liver and spleen CT ratio,Traditional Chinese medicine(TCM)syndrome,body mass index(BMI,WC,SMM,body fat percentage,visceral fat area),liver function,and blood lipid levels were compared before and after treatment,and the curative effect was evaluated;6 cases of NAFLD patients whose clinical efficacy after Huazhi Rougan granule intervention was effective were randomly selected,and the patient’s oral and intestinal samples were taken for further analysis.Results:After treatment,the total effective rate of clinical efficacy was 85.14%.The TCM symptoms of NAFLD patients were significantly alleviated compared with before treatment(P<0.05).There were statistically significant differences in ALT,AST,GGT,TC,and TG before and after treatment(P<0.01).After treatment,BW,BMI,WC,body fat percentage,visceral fat area,liver hardness,and fat attenuation index were significantly decreased(P<0.01).Oral-gut flora analysis showed:After treatment,the Shannon index of the gut microbial community increased(P<0.01).Compared with before treatment,the abundance of Firmicutes decreased,while the abundance of Bacteroidetes,Candidatus-Sa,and Tenericutes increased(P<0.05).At the genus level,the abundance of Bacteroides,Lactobacillus,and Bifidobacterium increased,and Streptococcus,Oribacterium,and Peptoanaerobacter decreased(P<0.05).Before and after treatment,the dominant bacterial phyla in the intestine were the same,including Bacteroidetes,Firmicutes,and Proteobacteria.The abundance of Firmicutes and Roseburia decreased after treatment(P<0.05).Conclusions:Huazhi Rougan granules have a significant effect on the treatment of NAFLD,can improve liver function,reduce blood lipids,can effectively reduce body morphological indicators such as body mass,WC,body fat percentage,visceral fat area,liver stiffness measurement(LSM),and fat content,and has the function of protecting the liver and reducing blood pressure,promoting the role of intrahepatic fat metabolism;and significantly relieve the clinical symptoms.NAFLD patients have the gut and oral flora disturbances.Huazhi Rougan granules can restore the diversity of intestinal microbial communities in NAFLD patients and play a therapeutic role by regulating the abundance and composition of oral and Gut flora.
基金Supported by Guangxi Key R&D Programme Project(GK AB21196057)National Old Pharmacist Inheritance Studio Construction Project.
文摘[Objectives] To establish the preparation process and quality standard of Rougan Huaxian ointment.[Methods] The L 9(3 4) orthogonal test was employed to optimize the preparation process by considering the multiplication of water addition, extraction time, and extraction frequency as influencing factors. The dry paste yield was utilized as the evaluation criterion, in conjunction with the actual production conditions. Thin layer chromatography was employed to identify Radix Astragali, Lycii Fructus, Herba Dendrobii, and Rhizoma Polygoni Cuspidati. Ethanol served as the solvent for the determination of ethanol-soluble extractives using the cold immersion method.[Results] The preparation process was conducted as follows: the specified quantity of medicinal materials was combined with water for extraction purposes, performed in two separate stages. In each stage, eight times the amount of water was added. The first extraction lasted for 1.5 h, while the second extraction was completed in 1.0 h. The resulting liquid was then concentrated into a thick paste with a relative density ranging from 1.25 to 1.30 at a temperature of 60 ℃. The thin-layer chromatography analysis of Radix Astragali, Lycii Fructus, Herba Dendrobii, and Rhizoma Polygoni Cuspidati demonstrated distinct spots, effective separation, and the absence of interference from negative samples. Additionally, the ethanol-soluble extractives yielded a minimum of 8.0% in terms of dry weight.[Conclusions] The preparation process for Rougan Huaxian ointment is both stable and feasible. Furthermore, the quality standards established for this preparation are unique and reproducible, thereby facilitating effective quality control.
文摘Objective: Huazhi Rougan granules(HRGs) are a promising drug to improve alcoholic liver disease(ALD), but its mechanism remains unclear. Materials and Methods: Network pharmacology, molecular docking, and animal experiments were used to reveal the potential mechanism of HRG against ALD. Results: A total of 245 potential targets of HRG against ALD were obtained. Functional enrichment analysis suggests that HRG could synergistically regulate various biological pathways to exert therapeutic effects on ALD. Molecular docking showed that the key targets exhibited the good binding ability with the key compounds. The in vivo experiments showed that HRG can effectively alleviate pathological changes in liver tissue, improve blood lipid levels, antioxidant stress ability, and liver function, and reduce the release of inflammatory cytokines in the liver. Conclusions: This study reflects that HRG is an effective strategy for treating ALD, providing a basis for revealing the prevention and treatment mechanisms of ALD.
