为鉴定鸡白痢沙门菌生物被膜形成不依赖Rpo S的调控基因,以鸡白痢沙门菌S6702及其rpoS基因缺失株S6702ΔrpoS为母本,对S6702生物被膜状态和S6702ΔrpoS生物被膜状态(S7BF vs S7SBF)及S6702ΔrpoS浮游状态和S6702ΔrpoS生物被膜状态(S7SF...为鉴定鸡白痢沙门菌生物被膜形成不依赖Rpo S的调控基因,以鸡白痢沙门菌S6702及其rpoS基因缺失株S6702ΔrpoS为母本,对S6702生物被膜状态和S6702ΔrpoS生物被膜状态(S7BF vs S7SBF)及S6702ΔrpoS浮游状态和S6702ΔrpoS生物被膜状态(S7SFY vs S7SBF)进行转录组测序分析,筛选差异表达基因,构建基因缺失株并验证其生物被膜形成能力。结果显示,S7BF vs S7SBF组共鉴定出83个差异表达基因,S7SFY vs S7SBF组共鉴定出1984个差异表达基因,筛选出15个可能与生物被膜形成相关的基因。以STM2361和STM1703为靶标构建基因缺失株,结晶紫染色定量结果显示,STM2361不影响鸡白痢沙门菌生物被膜的形成,而STM1703缺失能增强鸡白痢沙门菌的生物被膜形成能力。本研究鉴定出不依赖RpoS的鸡白痢沙门菌生物被膜形成调控基因,丰富了鸡白痢沙门菌生物被膜调控的信息。展开更多
The role ofEscherichia coli rpoS on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose- enriched culture conditions. The experimental results...The role ofEscherichia coli rpoS on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose- enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the rpoS-deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation ofgltA and one-tenth downregulation of poxB, respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing rpoS gene into the mutant. These results demonstrated rpoS changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.展开更多
基金Supported by Science and Technology Program of Shandong Province (No. 2010GHY10501)National Department Public Benefit Research Fond of China (No. 200909020)~~
文摘为鉴定鸡白痢沙门菌生物被膜形成不依赖Rpo S的调控基因,以鸡白痢沙门菌S6702及其rpoS基因缺失株S6702ΔrpoS为母本,对S6702生物被膜状态和S6702ΔrpoS生物被膜状态(S7BF vs S7SBF)及S6702ΔrpoS浮游状态和S6702ΔrpoS生物被膜状态(S7SFY vs S7SBF)进行转录组测序分析,筛选差异表达基因,构建基因缺失株并验证其生物被膜形成能力。结果显示,S7BF vs S7SBF组共鉴定出83个差异表达基因,S7SFY vs S7SBF组共鉴定出1984个差异表达基因,筛选出15个可能与生物被膜形成相关的基因。以STM2361和STM1703为靶标构建基因缺失株,结晶紫染色定量结果显示,STM2361不影响鸡白痢沙门菌生物被膜的形成,而STM1703缺失能增强鸡白痢沙门菌的生物被膜形成能力。本研究鉴定出不依赖RpoS的鸡白痢沙门菌生物被膜形成调控基因,丰富了鸡白痢沙门菌生物被膜调控的信息。
文摘The role ofEscherichia coli rpoS on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose- enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the rpoS-deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation ofgltA and one-tenth downregulation of poxB, respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing rpoS gene into the mutant. These results demonstrated rpoS changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.
