Our findings represent the first report of the antitumor activity of the disintegrin-like components from the venom of Armenian viper (M. raddei). The venom of M. raddei was separated by reverse phase high-performance...Our findings represent the first report of the antitumor activity of the disintegrin-like components from the venom of Armenian viper (M. raddei). The venom of M. raddei was separated by reverse phase high-performance liquid chroma-tography (RP HPLC), and individual fractions were analyzed for disintegrin activity. Disintegrin-like components from the venom of M. raddei, by blocking integrins on breast cancer cells (MDA-MB-435), not only interferes with adhesion of breast cancer cells to the extracellular matrix, but also inhibits cellular mobility which is essential for cancer invasion. These effects seriously curtail the metastatic capability of the MDA-MB-435 cells.展开更多
The present study was undertaken to elucidate how the plastic properties of model membranes from native lipids of different tissues of rats change in the course of Macrovipera lebetina obtuse (MLO), Montivipera raddei...The present study was undertaken to elucidate how the plastic properties of model membranes from native lipids of different tissues of rats change in the course of Macrovipera lebetina obtuse (MLO), Montivipera raddei (MR) and Naja kaouthia (NK) venoms processing. The presence of viper venom in organism lead to increasing of the electrical resistance of BLMs from liver and muscle lipids approximately on a sequence, while the BLMs from brain lipids has not shown a noticeable differences of plastic properties compare the control. The same concentration of cobra venom leads to decreasing of electrical resistance of BLMs from 1011 Ohm till 108 Ohm. The low concentration of venom leads to appearance of channel activity. Especially it is noticeable in liver lipids in media of bivalen ions.展开更多
目的观察常规凝血试验结果与血浆达比加群浓度的相关性,寻找合适的试验用于监测达比加群服药患者的出血风险。方法分析北京大学第三医院2015年至2017年使用达比加群抗凝的49名非瓣膜性心房颤动(NVAF)患者的临床资料,并计算其出血发生率...目的观察常规凝血试验结果与血浆达比加群浓度的相关性,寻找合适的试验用于监测达比加群服药患者的出血风险。方法分析北京大学第三医院2015年至2017年使用达比加群抗凝的49名非瓣膜性心房颤动(NVAF)患者的临床资料,并计算其出血发生率。收集20名健康志愿者的血浆,制成正常混合血浆(NPP),分别向1 m L NPP中加入0~1 000 ng/m L浓度梯度的达比加群,检测凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(Fib)和稀释蝰蛇毒时间(dRVVT)。另外收集3名健康志愿者的新鲜全血,做相同的加药处理后进行血栓弹力图试验(TEG)。结果服用达比加群的NVAF患者的出血发生率高达28.6%,以消化道出血居多。APTT、dRVVT、TEG的R时间(R)和凝血指数(CI)结果与血浆达比加群浓度相关性良好,其中,dRVVT的敏感性最强,APTT次之,R和CI敏感性较差。结论 APTT和dRVVT在监测达比加群出血事件方面具有重要意义,将有助于临床寻找合适的停药时机,减少出血事件的发生。展开更多
目的:探究狼疮抗凝物检测中稀释蝰蛇毒时间(diluted Russell viper venom time, d RVVT)法与混合试验(Rosner指数)检测结果间的相关性及方法学特点。方法:2015年9月至2019年9月期间,收集在上海交通大学医学院附属瑞金医院就诊的31例凝...目的:探究狼疮抗凝物检测中稀释蝰蛇毒时间(diluted Russell viper venom time, d RVVT)法与混合试验(Rosner指数)检测结果间的相关性及方法学特点。方法:2015年9月至2019年9月期间,收集在上海交通大学医学院附属瑞金医院就诊的31例凝血功能异常、狼疮抗凝物阳性患者的临床资料,比较d RVVT标准化比值与混合试验(Rosner指数的R1、R2)检测结果间的相关性,进而探究2种方法间的异同。结果:从31例患者dRVVT标准化比值与病人血浆(病浆)活化部分凝血活酶时间(activated partial thromboplastin time,APTT)值与混合试验的R1、R2的散点图可以看出,d RVVT标准化比值分别与病浆APTT值、R1及R2之间具有较好的相关性。进一步应用Pearson相关性分析得出,dRVVT标准化比值与混合试验检测结果显著相关(相关系数r值为0.54~0.62,P<0.05)。结论:基于共同凝血途径d RVVT法的确证试验与混合试验(基于内源性及共同凝血途径)结果间具有显著相关性,但并非严格的线性相关。2种检测方法在狼疮抗凝物检测中并不可完全替代,因此仍建议将二者相结合进行分析,从而提高狼疮抗凝物的检出率,降低误诊率和漏诊率。展开更多
文摘Our findings represent the first report of the antitumor activity of the disintegrin-like components from the venom of Armenian viper (M. raddei). The venom of M. raddei was separated by reverse phase high-performance liquid chroma-tography (RP HPLC), and individual fractions were analyzed for disintegrin activity. Disintegrin-like components from the venom of M. raddei, by blocking integrins on breast cancer cells (MDA-MB-435), not only interferes with adhesion of breast cancer cells to the extracellular matrix, but also inhibits cellular mobility which is essential for cancer invasion. These effects seriously curtail the metastatic capability of the MDA-MB-435 cells.
文摘The present study was undertaken to elucidate how the plastic properties of model membranes from native lipids of different tissues of rats change in the course of Macrovipera lebetina obtuse (MLO), Montivipera raddei (MR) and Naja kaouthia (NK) venoms processing. The presence of viper venom in organism lead to increasing of the electrical resistance of BLMs from liver and muscle lipids approximately on a sequence, while the BLMs from brain lipids has not shown a noticeable differences of plastic properties compare the control. The same concentration of cobra venom leads to decreasing of electrical resistance of BLMs from 1011 Ohm till 108 Ohm. The low concentration of venom leads to appearance of channel activity. Especially it is noticeable in liver lipids in media of bivalen ions.
文摘目的观察常规凝血试验结果与血浆达比加群浓度的相关性,寻找合适的试验用于监测达比加群服药患者的出血风险。方法分析北京大学第三医院2015年至2017年使用达比加群抗凝的49名非瓣膜性心房颤动(NVAF)患者的临床资料,并计算其出血发生率。收集20名健康志愿者的血浆,制成正常混合血浆(NPP),分别向1 m L NPP中加入0~1 000 ng/m L浓度梯度的达比加群,检测凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(Fib)和稀释蝰蛇毒时间(dRVVT)。另外收集3名健康志愿者的新鲜全血,做相同的加药处理后进行血栓弹力图试验(TEG)。结果服用达比加群的NVAF患者的出血发生率高达28.6%,以消化道出血居多。APTT、dRVVT、TEG的R时间(R)和凝血指数(CI)结果与血浆达比加群浓度相关性良好,其中,dRVVT的敏感性最强,APTT次之,R和CI敏感性较差。结论 APTT和dRVVT在监测达比加群出血事件方面具有重要意义,将有助于临床寻找合适的停药时机,减少出血事件的发生。
文摘目的:探究狼疮抗凝物检测中稀释蝰蛇毒时间(diluted Russell viper venom time, d RVVT)法与混合试验(Rosner指数)检测结果间的相关性及方法学特点。方法:2015年9月至2019年9月期间,收集在上海交通大学医学院附属瑞金医院就诊的31例凝血功能异常、狼疮抗凝物阳性患者的临床资料,比较d RVVT标准化比值与混合试验(Rosner指数的R1、R2)检测结果间的相关性,进而探究2种方法间的异同。结果:从31例患者dRVVT标准化比值与病人血浆(病浆)活化部分凝血活酶时间(activated partial thromboplastin time,APTT)值与混合试验的R1、R2的散点图可以看出,d RVVT标准化比值分别与病浆APTT值、R1及R2之间具有较好的相关性。进一步应用Pearson相关性分析得出,dRVVT标准化比值与混合试验检测结果显著相关(相关系数r值为0.54~0.62,P<0.05)。结论:基于共同凝血途径d RVVT法的确证试验与混合试验(基于内源性及共同凝血途径)结果间具有显著相关性,但并非严格的线性相关。2种检测方法在狼疮抗凝物检测中并不可完全替代,因此仍建议将二者相结合进行分析,从而提高狼疮抗凝物的检出率,降低误诊率和漏诊率。
