Individual variation in the response to drug therapy has been mainly attributed to the genetic polymorphism of cytochrome P 450 isoenzymes. Mutation in the gene CYP2C19 （cytochrome P450 2C19） has been shown to influ...Individual variation in the response to drug therapy has been mainly attributed to the genetic polymorphism of cytochrome P 450 isoenzymes. Mutation in the gene CYP2C19 （cytochrome P450 2C19） has been shown to influence the clinical efficiency of clopidogrel. The aim is to investigate the frequencies of CYP2C19＊2 （c.G681A; rs4244285）, CYP2C19＊3 （c.G636A; rs4986893）, CYP2C19＊17 （c.C806T; rs12248560） and to compare the allele and genotype frequencies of CYP2C19＊2 in patients with CHD （coronary heart disease） to healthy volunteers. We examined 53 patients with CHD received clopidogrel and 146 healthy volunteers. CYP2C19＊2, CYP2C19＊3, CYP2C19＊17 carriages were determined by a polymerase-chain reaction. The observed genotype distribution did not deviate from Hardy-Weinberg equilibrium, it was determined by a Chi-square test with Yates correction. The frequency of CYP2C19＂2 allele reported in patients with CHD and in the healthy volunteers was 16.6% and 13.3%, respectively （P = 0.584）. The results of the present study may be helpful in developing current and future directions for its management.展开更多
We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat(STR)loci and the amelogenin locus,which are not included in the combined DNA index system(CODIS),in a Ru...We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat(STR)loci and the amelogenin locus,which are not included in the combined DNA index system(CODIS),in a Russian ethnic minority group from the Inner Mongolia Autonomous Region,China.A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction(PCR)system.Using capillary electrophoresis,the PCR products of the 21 STR loci were separated and genotyped.A total of 161 alleles were observed in the Russian ethnic minority group,and corresponding allelic frequencies ranged from 0.0044 to 0.5965.The 21 non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population's genetic background,for individual identification,and for paternity testing in forensic practice.展开更多
文摘Individual variation in the response to drug therapy has been mainly attributed to the genetic polymorphism of cytochrome P 450 isoenzymes. Mutation in the gene CYP2C19 （cytochrome P450 2C19） has been shown to influence the clinical efficiency of clopidogrel. The aim is to investigate the frequencies of CYP2C19＊2 （c.G681A; rs4244285）, CYP2C19＊3 （c.G636A; rs4986893）, CYP2C19＊17 （c.C806T; rs12248560） and to compare the allele and genotype frequencies of CYP2C19＊2 in patients with CHD （coronary heart disease） to healthy volunteers. We examined 53 patients with CHD received clopidogrel and 146 healthy volunteers. CYP2C19＊2, CYP2C19＊3, CYP2C19＊17 carriages were determined by a polymerase-chain reaction. The observed genotype distribution did not deviate from Hardy-Weinberg equilibrium, it was determined by a Chi-square test with Yates correction. The frequency of CYP2C19＂2 allele reported in patients with CHD and in the healthy volunteers was 16.6% and 13.3%, respectively （P = 0.584）. The results of the present study may be helpful in developing current and future directions for its management.
基金Project supported by the Program for New Century Excellent Talents of the Ministry of Education,China (No. NECT-10-0687)the Fun-damental Research Funds for the Central University (No. 2011jdgz20)the Shaanxi Provincial Science and Technology Research and Development Project Fund (No. 2012K16-08-01),China
文摘We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat(STR)loci and the amelogenin locus,which are not included in the combined DNA index system(CODIS),in a Russian ethnic minority group from the Inner Mongolia Autonomous Region,China.A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction(PCR)system.Using capillary electrophoresis,the PCR products of the 21 STR loci were separated and genotyped.A total of 161 alleles were observed in the Russian ethnic minority group,and corresponding allelic frequencies ranged from 0.0044 to 0.5965.The 21 non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population's genetic background,for individual identification,and for paternity testing in forensic practice.
