Mitochondrial dysfunction is a hallmark of Alzheimer’s disease.We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 mice.Because Alzheime...Mitochondrial dysfunction is a hallmark of Alzheimer’s disease.We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 mice.Because Alzheimer’s disease affects the entire brain,further research is needed to elucidate alterations in mitochondrial metabolism in the brain as a whole.Here,we investigated the expression of several important mitochondrial biogenesis-related cytokines in multiple brain regions after treatment with neural stem cell-derived exosomes and used a combination of whole brain clearing,immunostaining,and lightsheet imaging to clarify their spatial distribution.Additionally,to clarify whether the sirtuin 1(SIRT1)-related pathway plays a regulatory role in neural stem cell-de rived exosomes interfering with mitochondrial functional changes,we generated a novel nervous system-SIRT1 conditional knoc kout AP P/PS1mouse model.Our findings demonstrate that neural stem cell-de rived exosomes significantly increase SIRT1 levels,enhance the production of mitochondrial biogenesis-related fa ctors,and inhibit astrocyte activation,but do not suppress amyloid-βproduction.Thus,neural stem cell-derived exosomes may be a useful therapeutic strategy for Alzheimer’s disease that activates the SIRT1-PGC1αsignaling pathway and increases NRF1 and COXIV synthesis to improve mitochondrial biogenesis.In addition,we showed that the spatial distribution of mitochondrial biogenesis-related factors is disrupted in Alzheimer’s disease,and that neural stem cell-derived exosome treatment can reverse this effect,indicating that neural stem cell-derived exosomes promote mitochondrial biogenesis.展开更多
The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal degeneration.However,it remains largely unclear how PINK1 and Parkin a...The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal degeneration.However,it remains largely unclear how PINK1 and Parkin are expressed in mammalian brains.This has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small animals.Understanding this issue is critical for elucidating the in vivo roles of PINK1 and Parkin.Recently,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate brain.In the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate brain.We found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey brains.PINK1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable distribution.Immunofluorescent double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial stress.These findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.展开更多
Ambient noise tomography,when applied to a dense linear seismic array,has the capability to provide detailed insights into the fine velocity structures across diverse tectonic settings.The linear station arrangement n...Ambient noise tomography,when applied to a dense linear seismic array,has the capability to provide detailed insights into the fine velocity structures across diverse tectonic settings.The linear station arrangement naturally generates parallel and concentrated ray paths along the array trend.This unique geometry requires specific optimization of the inversion methodology and model parameterization.The Bayesian-based transdimensional inversion method,characterized by its fully non-linear nature and high degree of freedom in parameter settings,offers a powerful tool for ambient noise inversion.To effectively adapt this method to a linear array layout,we propose a modification to the Voronoi cell tessellation built in the transdimensional method.By introducing spatial priority to the Voronoi kernels,we strategically increased the density of Voronoi cells along the direction of the array.We then applied the modified approach to a linear seismic array in the North China Craton and validated its robustness through phase velocity images and resolution tests.Our improved non-uniform sampling technique in the 2-D model space accelerates convergence while simultaneously enhancing model accuracy.Compared with the conventional damped leastsquares method,the proposed algorithm revealed a shear-wave velocity map with notable low-velocity anomalies situated in the middle and lower crust beneath the borders of the Ordos block and its surrounding orogenic belt.Aligned with the crustal structures revealed by receiver function and electrical imaging,our findings indicated that the western and eastern margins of the Ordos block had experienced intensive crustal wedge deformation and re-melting,respectively.展开更多
Turbine noise would be one of the dominant noise sources especially in future UHBR (Ultra High Bypass-Ratio) aeroengine, but its currently far from being studied enough. Acoustic mode is crucial for duct propagation b...Turbine noise would be one of the dominant noise sources especially in future UHBR (Ultra High Bypass-Ratio) aeroengine, but its currently far from being studied enough. Acoustic mode is crucial for duct propagation but little study about the relation between serration and mode. Thus, taking axial single-turbine test bench NPU-Turb as object, the effect of Stator with Serrated Trailing-edge (Bionic S) and Rotor with Serrated Leading-edge (Bionic R) on duct acoustic modes of turbine turbulence interac-tion noise were studied in detail using DDES/AA hybrid model validated by acoustic experiment of NPU-Turb. Serval conclusions can be made here. First, for broadband noise, the effect of serrations on duct modes (increased or reduced of PWL<sub>mn</sub>) with the increasing frequency is more prominent. Second, the changing trend of ?PWL<sub>mn</sub> is something like Chinese character “人” with circumferential mode m and alternating with radial mode. Such distribution is more obvious at higher frequency. More theoretical and mechanistic research work needs to be carried out in depth in the future. .展开更多
Acceptance sampling is used to decide either the whole lot will be accepted or rejected,based on inspection of randomly sampled items from the same lot.As an alternative to traditional sampling plans,it is possible to...Acceptance sampling is used to decide either the whole lot will be accepted or rejected,based on inspection of randomly sampled items from the same lot.As an alternative to traditional sampling plans,it is possible to use Baye-sian approaches using previous knowledge on process variation.This study pre-sents a Bayesian two-sided group chain sampling plan(BTSGChSP)by using various combinations of design parameters.In BTSGChSP,inspection is based on preceding as well as succeeding lots.Poisson function is used to derive the probability of lot acceptance based on defective and non-defective products.Gamma distribution is considered as a suitable prior for Poisson distribution.Four quality regions are found,namely:(i)quality decision region(QDR),(ii)probabil-istic quality region(PQR),(iii)limiting quality region(LQR)and(iv)indifference quality region(IQR).Producer’s risk and consumer’s risk are considered to esti-mate the quality regions,where acceptable quality level(AQL)is associated with producer’s risk and limiting quality level(LQL)is associated with consumer’s risk.Moreover,AQL and LQL are used in the selection of design parameters for BTSGChSP.The values based on all possible combinations of design parameters for BTSGChSP are presented and inflection points’values are found.Thefinding exposes that BTSGChSP is a better substitute for the existing plan for industrial practitioners.展开更多
Understanding the composition and contents of carotenoids in various soybean seed accessions is important for their nutritional assessment.This study investigated the variability in the concentrations of carotenoids a...Understanding the composition and contents of carotenoids in various soybean seed accessions is important for their nutritional assessment.This study investigated the variability in the concentrations of carotenoids and chlorophylls and revealed their associations with other nutritional quality traits in a genetically diverse set of Chinese soybean accessions comprised of cultivars and landraces.Genotype,planting year,accession type,seed cotyledon color,and ecoregion of origin significantly influenced the accumulation of carotenoids and chlorophylls.The mean total carotenoid content was in the range of 8.15–14.72μg g–1 across the ecoregions.The total carotenoid content was 1.2-fold higher in the landraces than in the cultivars.Soybeans with green cotyledons had higher contents of carotenoids and chlorophylls than those with yellow cotyledons.Remarkably,lutein was the most abundant carotenoid in all the germplasms,ranging from 1.35–37.44μg g–1.Carotenoids and chlorophylls showed significant correlations with other quality traits,which will help to set breeding strategies for enhancing soybean carotenoids without affecting the other components.Collectively,our results demonstrate that carotenoids are adequately accumulated in soybean seeds,however,they are strongly influenced by genetic factors,accession type,and germplasm origin.We identified novel germplasms with the highest total carotenoid contents across the various ecoregions of China that could serve as the genetic materials for soybean carotenoid breeding programs,and thereby as the raw materials for food sectors,pharmaceuticals,and the cosmetic industry.展开更多
The diameter distribution of trees in a stand provides the basis for determining the stand’s ecological and economic value,its structure and stability and appropriate management practices.Scots pine(Pinus sylvestris ...The diameter distribution of trees in a stand provides the basis for determining the stand’s ecological and economic value,its structure and stability and appropriate management practices.Scots pine(Pinus sylvestris L.)is one of the most common and important conifers in Turkey,so a well-planned management schedule is critical.Diameter distribution models to accurately describe the stand structure help improve management strategies,but developing reliable models requires a deep understanding of the growth,output and constraints of the forests.The most important information derived by diameter distribution models is primary data on horizontal stand structure for each diameter class of trees:basal area and volume per unit area.These predictions are required to estimate the range of products and predicted volume and yield from a forest stand.Here,to construct an accurate,reliable diameter distribution model for natural Scots pine stands in the Türkmen Mountain region,we used Johnson’s SBdistribution to represent the empirical diameter distributions of the stands using ground-based measurements from 55 sample plots that included1219 trees in natural distribution zones of the forests.As an alternative,nonparametric approach,which does not require any predefined function,an artificial intelligence model was constructed based on support vector machine methodology.An error index was calculated to evaluate the results.Overall,both Johnson’s SB probability density function with a three-parameter recovery approach and the support vector regression methodology provided reliable estimates of the diameter distribution of these stands.展开更多
Based on one type of practical Biot＇s equation and the dynamic-stiffness matrices ofa poroelastic soil layer and half-space, Green＇s functions were derived for unitformly distributed loads acting on an inclined line...Based on one type of practical Biot＇s equation and the dynamic-stiffness matrices ofa poroelastic soil layer and half-space, Green＇s functions were derived for unitformly distributed loads acting on an inclined line in a poroelastie layered site. This analysis overcomes significant problems in wave scattering due to local soil conditions and dynamic soil-structure interaction. The Green＇s functions can be reduced to the case of an elastic layered site developed by Wolf in 1985. Parametric studies are then carried out through two example problems.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82171194 and 81974155(both to JL)the Shanghai Municipal Science and Technology Commission Medical Guide Project,No.16411969200(to WZ)Shanghai Municipal Science and Technology Commission Biomedical Science and Technology Project,No.22S31902600(to JL)。
文摘Mitochondrial dysfunction is a hallmark of Alzheimer’s disease.We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 mice.Because Alzheimer’s disease affects the entire brain,further research is needed to elucidate alterations in mitochondrial metabolism in the brain as a whole.Here,we investigated the expression of several important mitochondrial biogenesis-related cytokines in multiple brain regions after treatment with neural stem cell-derived exosomes and used a combination of whole brain clearing,immunostaining,and lightsheet imaging to clarify their spatial distribution.Additionally,to clarify whether the sirtuin 1(SIRT1)-related pathway plays a regulatory role in neural stem cell-de rived exosomes interfering with mitochondrial functional changes,we generated a novel nervous system-SIRT1 conditional knoc kout AP P/PS1mouse model.Our findings demonstrate that neural stem cell-de rived exosomes significantly increase SIRT1 levels,enhance the production of mitochondrial biogenesis-related fa ctors,and inhibit astrocyte activation,but do not suppress amyloid-βproduction.Thus,neural stem cell-derived exosomes may be a useful therapeutic strategy for Alzheimer’s disease that activates the SIRT1-PGC1αsignaling pathway and increases NRF1 and COXIV synthesis to improve mitochondrial biogenesis.In addition,we showed that the spatial distribution of mitochondrial biogenesis-related factors is disrupted in Alzheimer’s disease,and that neural stem cell-derived exosome treatment can reverse this effect,indicating that neural stem cell-derived exosomes promote mitochondrial biogenesis.
基金supported by the National Natural Science Foundation of China,Nos.32070534(to WY),32370567(to WY),82371874(to XL),81830032(to XL),82071421(to SL)Key Field Research and Development Program of Guangdong Province,No.2018B030337001(to XL)+2 种基金Guangzhou Key Research Program on Brain Science,No.202007030008(to XL)Department of Science and Technology of Guangdong Province,Nos.2021ZT09Y007,2020B121201006(to XL)Guangdong Basic and Applied Basic Research Foundation,Nos.2022A1515012301(to WY),2023B1515020031(to WY).
文摘The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal degeneration.However,it remains largely unclear how PINK1 and Parkin are expressed in mammalian brains.This has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small animals.Understanding this issue is critical for elucidating the in vivo roles of PINK1 and Parkin.Recently,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate brain.In the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate brain.We found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey brains.PINK1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable distribution.Immunofluorescent double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial stress.These findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.
基金funded by the Special Fund of the Institute of Geophysics,China Earthquake Administration (Nos.DQJB21K52,and DQJB22R33)。
文摘Ambient noise tomography,when applied to a dense linear seismic array,has the capability to provide detailed insights into the fine velocity structures across diverse tectonic settings.The linear station arrangement naturally generates parallel and concentrated ray paths along the array trend.This unique geometry requires specific optimization of the inversion methodology and model parameterization.The Bayesian-based transdimensional inversion method,characterized by its fully non-linear nature and high degree of freedom in parameter settings,offers a powerful tool for ambient noise inversion.To effectively adapt this method to a linear array layout,we propose a modification to the Voronoi cell tessellation built in the transdimensional method.By introducing spatial priority to the Voronoi kernels,we strategically increased the density of Voronoi cells along the direction of the array.We then applied the modified approach to a linear seismic array in the North China Craton and validated its robustness through phase velocity images and resolution tests.Our improved non-uniform sampling technique in the 2-D model space accelerates convergence while simultaneously enhancing model accuracy.Compared with the conventional damped leastsquares method,the proposed algorithm revealed a shear-wave velocity map with notable low-velocity anomalies situated in the middle and lower crust beneath the borders of the Ordos block and its surrounding orogenic belt.Aligned with the crustal structures revealed by receiver function and electrical imaging,our findings indicated that the western and eastern margins of the Ordos block had experienced intensive crustal wedge deformation and re-melting,respectively.
文摘Turbine noise would be one of the dominant noise sources especially in future UHBR (Ultra High Bypass-Ratio) aeroengine, but its currently far from being studied enough. Acoustic mode is crucial for duct propagation but little study about the relation between serration and mode. Thus, taking axial single-turbine test bench NPU-Turb as object, the effect of Stator with Serrated Trailing-edge (Bionic S) and Rotor with Serrated Leading-edge (Bionic R) on duct acoustic modes of turbine turbulence interac-tion noise were studied in detail using DDES/AA hybrid model validated by acoustic experiment of NPU-Turb. Serval conclusions can be made here. First, for broadband noise, the effect of serrations on duct modes (increased or reduced of PWL<sub>mn</sub>) with the increasing frequency is more prominent. Second, the changing trend of ?PWL<sub>mn</sub> is something like Chinese character “人” with circumferential mode m and alternating with radial mode. Such distribution is more obvious at higher frequency. More theoretical and mechanistic research work needs to be carried out in depth in the future. .
基金supported by the Ministry of Higher Education(MoHE)through Fundamental Research Grant Scheme(FRGS/1/2020/STG06/UUM/02/2).
文摘Acceptance sampling is used to decide either the whole lot will be accepted or rejected,based on inspection of randomly sampled items from the same lot.As an alternative to traditional sampling plans,it is possible to use Baye-sian approaches using previous knowledge on process variation.This study pre-sents a Bayesian two-sided group chain sampling plan(BTSGChSP)by using various combinations of design parameters.In BTSGChSP,inspection is based on preceding as well as succeeding lots.Poisson function is used to derive the probability of lot acceptance based on defective and non-defective products.Gamma distribution is considered as a suitable prior for Poisson distribution.Four quality regions are found,namely:(i)quality decision region(QDR),(ii)probabil-istic quality region(PQR),(iii)limiting quality region(LQR)and(iv)indifference quality region(IQR).Producer’s risk and consumer’s risk are considered to esti-mate the quality regions,where acceptable quality level(AQL)is associated with producer’s risk and limiting quality level(LQL)is associated with consumer’s risk.Moreover,AQL and LQL are used in the selection of design parameters for BTSGChSP.The values based on all possible combinations of design parameters for BTSGChSP are presented and inflection points’values are found.Thefinding exposes that BTSGChSP is a better substitute for the existing plan for industrial practitioners.
基金financially supported by the National Natural Science Foundation of China(32161143033 and 32001574)the Agricultural Science and Technology Innovation Program of CAAS(2060203-2).
文摘Understanding the composition and contents of carotenoids in various soybean seed accessions is important for their nutritional assessment.This study investigated the variability in the concentrations of carotenoids and chlorophylls and revealed their associations with other nutritional quality traits in a genetically diverse set of Chinese soybean accessions comprised of cultivars and landraces.Genotype,planting year,accession type,seed cotyledon color,and ecoregion of origin significantly influenced the accumulation of carotenoids and chlorophylls.The mean total carotenoid content was in the range of 8.15–14.72μg g–1 across the ecoregions.The total carotenoid content was 1.2-fold higher in the landraces than in the cultivars.Soybeans with green cotyledons had higher contents of carotenoids and chlorophylls than those with yellow cotyledons.Remarkably,lutein was the most abundant carotenoid in all the germplasms,ranging from 1.35–37.44μg g–1.Carotenoids and chlorophylls showed significant correlations with other quality traits,which will help to set breeding strategies for enhancing soybean carotenoids without affecting the other components.Collectively,our results demonstrate that carotenoids are adequately accumulated in soybean seeds,however,they are strongly influenced by genetic factors,accession type,and germplasm origin.We identified novel germplasms with the highest total carotenoid contents across the various ecoregions of China that could serve as the genetic materials for soybean carotenoid breeding programs,and thereby as the raw materials for food sectors,pharmaceuticals,and the cosmetic industry.
基金supported by Turkish General Directorate of Forestry。
文摘The diameter distribution of trees in a stand provides the basis for determining the stand’s ecological and economic value,its structure and stability and appropriate management practices.Scots pine(Pinus sylvestris L.)is one of the most common and important conifers in Turkey,so a well-planned management schedule is critical.Diameter distribution models to accurately describe the stand structure help improve management strategies,but developing reliable models requires a deep understanding of the growth,output and constraints of the forests.The most important information derived by diameter distribution models is primary data on horizontal stand structure for each diameter class of trees:basal area and volume per unit area.These predictions are required to estimate the range of products and predicted volume and yield from a forest stand.Here,to construct an accurate,reliable diameter distribution model for natural Scots pine stands in the Türkmen Mountain region,we used Johnson’s SBdistribution to represent the empirical diameter distributions of the stands using ground-based measurements from 55 sample plots that included1219 trees in natural distribution zones of the forests.As an alternative,nonparametric approach,which does not require any predefined function,an artificial intelligence model was constructed based on support vector machine methodology.An error index was calculated to evaluate the results.Overall,both Johnson’s SB probability density function with a three-parameter recovery approach and the support vector regression methodology provided reliable estimates of the diameter distribution of these stands.
基金National Natural Science Foundation of China Under Grant No.50378063
文摘Based on one type of practical Biot＇s equation and the dynamic-stiffness matrices ofa poroelastic soil layer and half-space, Green＇s functions were derived for unitformly distributed loads acting on an inclined line in a poroelastie layered site. This analysis overcomes significant problems in wave scattering due to local soil conditions and dynamic soil-structure interaction. The Green＇s functions can be reduced to the case of an elastic layered site developed by Wolf in 1985. Parametric studies are then carried out through two example problems.
