Based on analysis of the development potential of rangeland resources in China＇s mountain areas, the paper makes analysis and forecast in human carrying capacity of rangeland resources. It provides some reference for...Based on analysis of the development potential of rangeland resources in China＇s mountain areas, the paper makes analysis and forecast in human carrying capacity of rangeland resources. It provides some reference for drawing up the development strategy in mountain areas.展开更多
The issue of the new standards for employees＇ salary and general financial rules that include employees＇ salary and relevant expenditures, have a great impact on human resources cost accounting. This paper performs ...The issue of the new standards for employees＇ salary and general financial rules that include employees＇ salary and relevant expenditures, have a great impact on human resources cost accounting. This paper performs a specialized study on human resources cost and employees＇ salary, accounts setting and accounting treatment for human resources cost accounting, human resources cost report, etc. on the basis of relevant provisions in the new Chinese accounting standards, the general financial rules, international accounting standards, and relevant academic research findings.展开更多
With the increasingly severe global warming, investments in clean technology, reforestation and political action have been studied to reduce CO2 emission. In this study, a nonlinear stochastic model is proposed to des...With the increasingly severe global warming, investments in clean technology, reforestation and political action have been studied to reduce CO2 emission. In this study, a nonlinear stochastic model is proposed to describe the dynamics of CO2 emission with control inputs: clean technology, reforestation and carbon tax, under stochastic uncertainties. For the efficient resources management, a robust tracking control is designed to force resources tracking a desired reference output. The worst-case effect of stochastic parametric fluctuations, external disturbances and uncertain initial conditions on the tracking performance is considered and minimized from the dynamic game theory perspective. This stochastic game problem, in which one player (stochastic uncertainty) maximizes the tracking error and another player (control input) minimizes the tracking error, could be equivalent to a robust minimax tracking problem. To avoid solving the HJI, a fuzzy model is proposed to approximate the nonlinear CO2 emission model. Then the nonlinear stochastic game problem could be easily solved by fuzzy stochastic game approach via LMI technique.展开更多
AIM: The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS: The effect of AFP on the...AIM: The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS: The effect of AFP on the proliferation of NIH 3T3 cells was measured by incorporation of 3H-TdR. Receptor-binding assay of 125I-AFP was performed to detect the properties of AFP receptor in NIH 3T3 cells. The influences of AFP on the [cAMP]i and the activities of protein kinase A (PKA) were determined. Western blot was used to detect the change of K-ras P21 protein expression. RESULTS: The proliferation of NIH 3T3 cells treated with 0-80 mg/L of AFP was significantly enhanced. The Scatchard analysis indicated that there were two classes of binding sites with KD of 2.722 x 10(-9)M (Bmax=12810 sites per cell) and 8.931 x 10(-8)M (Bmax=119700 sites per cell) respectively. In the presence of AFP (20 mg/L), the content of cAMP and activities of PKA were significantly elevated . The level of K-ras P21 protein was upregulated by AFP at the concentration of 20 mg/L. The monoclonal antibody against AFP could reverse the effects of AFP on the cAMP content, PKA activity and the expression of K-ras p21 gene. CONCLUSION: The effect of AFP on the cell proliferation was achieved by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 gene.展开更多
AIM: To clone the cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) into a tetracycline-regulated gene expression system, the RevTet-On system, transduce expression vectors into a gastric carcinoma ...AIM: To clone the cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) into a tetracycline-regulated gene expression system, the RevTet-On system, transduce expression vectors into a gastric carcinoma cell line-NCI-N87 and examine the effects of controlled expression of TRAIL in vitro on the gastric carcinoma cells. METHODS: The full-length cDNA of TRAIL was inserted into a vector under the control of the tetracycline-responsive element (TRE) to obtain the plasmid pRevTRE-TRAIL, which was transfected into a packaging cell line PT67. In addition, vector pRev-Tet On and pRevTRE were also transfected into PT67 separately. After hygromycin and G418 selection, the viral titer was determined. The medium containing retroviral vectors was collected and used to transduce a gastric carcinoma cell line NCI-N87. The resulting cell line NCI-N87-Tet On TRE-TRAIL and a control cell line, NCI-N87 Tet On-TRE, were established. TRAIL expression in the cell line was induced by incubating cells with doxycycline (Dox), which is a tetracycline analogue. The killing effect on gastric carcinoma cells was analyzed after induction. RESULTS: The recombinant plasmid pRev-TRE-TRAIL was constructed. After hygromycin or G418 selection, the producer cell lines PT67-TRE, PT67-TRE-TRAIL and PT67-Tet On were obtained,with titers of about 10(8)CFU.L(-1). By transducing NCI-N87 cells with retroviral vectors from these cell lines, stable cell lines NCI-N87-Tet-On TRE-TRAIL (NN3T) and control cell line NCI-N87-Tet-On-TRE (NN2T) were established. The growth curves of the selected cell lines were the same with the wild type NCI-N87. When Dox was added, cell death was obvious in the test groups (29%-77%), whereas no difference was observed in control and wild type cell lines. With the addition of a medium from the test group, human leukemia cell line Jurkat was activated till death (83%), indicating the secretion of active TRAIL proteins from the test cells to the medium. CONCLUSION: With the use of the RevTet-On system, a regulated expression system for TRAIL was constructed. Using this system, the selected killing effect of TRAIL on gastric carcinoma cell line NCI-N87 could be observed.展开更多
The paper makes an empirical analysis on the main factors affecting IT corporate growth-human resource and R＆D. By using data of China＇s listed IT companies, we focus on the relationship among corporate value （net ...The paper makes an empirical analysis on the main factors affecting IT corporate growth-human resource and R＆D. By using data of China＇s listed IT companies, we focus on the relationship among corporate value （net return of equity, ROE） or development of COl＇porate value （Tobin＇s Q） and manager＇s salary, R＆D fee, R＆D employee etc. The conclusion shows that development of corporate value （Tobin＇s Q） has correlativity positive with the plurality of board chairman and general manager, independent directors＇ proportion, R＆D fee, and R＆D employees while it is non-obvious positive with salary of superior managers, holding stocks of general manager, capital investment, and negative with corporate scale. The conclusion is accordant with the development status of Chinese IT corporate.展开更多
Nowadays,the world belongs to the era of the economy full of information. The development of human resources in the entel-prise also shows the increasingly important. At present, commercial banks are not only to recru...Nowadays,the world belongs to the era of the economy full of information. The development of human resources in the entel-prise also shows the increasingly important. At present, commercial banks are not only to recruitment, introduction of outstanding professionals to enrich their team, but also the more important is potental of the existing management and staff training. Therefore, staff training and development will highlight out from the commercial bank management, which can make sure that a business adapt to the environment, realizing the fundamental value and the main enterprises for sustainable development.展开更多
文摘Based on analysis of the development potential of rangeland resources in China＇s mountain areas, the paper makes analysis and forecast in human carrying capacity of rangeland resources. It provides some reference for drawing up the development strategy in mountain areas.
文摘The issue of the new standards for employees＇ salary and general financial rules that include employees＇ salary and relevant expenditures, have a great impact on human resources cost accounting. This paper performs a specialized study on human resources cost and employees＇ salary, accounts setting and accounting treatment for human resources cost accounting, human resources cost report, etc. on the basis of relevant provisions in the new Chinese accounting standards, the general financial rules, international accounting standards, and relevant academic research findings.
文摘With the increasingly severe global warming, investments in clean technology, reforestation and political action have been studied to reduce CO2 emission. In this study, a nonlinear stochastic model is proposed to describe the dynamics of CO2 emission with control inputs: clean technology, reforestation and carbon tax, under stochastic uncertainties. For the efficient resources management, a robust tracking control is designed to force resources tracking a desired reference output. The worst-case effect of stochastic parametric fluctuations, external disturbances and uncertain initial conditions on the tracking performance is considered and minimized from the dynamic game theory perspective. This stochastic game problem, in which one player (stochastic uncertainty) maximizes the tracking error and another player (control input) minimizes the tracking error, could be equivalent to a robust minimax tracking problem. To avoid solving the HJI, a fuzzy model is proposed to approximate the nonlinear CO2 emission model. Then the nonlinear stochastic game problem could be easily solved by fuzzy stochastic game approach via LMI technique.
基金This work was supported by National NaturalScience Fundation of China(No.39760077).
文摘AIM: The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS: The effect of AFP on the proliferation of NIH 3T3 cells was measured by incorporation of 3H-TdR. Receptor-binding assay of 125I-AFP was performed to detect the properties of AFP receptor in NIH 3T3 cells. The influences of AFP on the [cAMP]i and the activities of protein kinase A (PKA) were determined. Western blot was used to detect the change of K-ras P21 protein expression. RESULTS: The proliferation of NIH 3T3 cells treated with 0-80 mg/L of AFP was significantly enhanced. The Scatchard analysis indicated that there were two classes of binding sites with KD of 2.722 x 10(-9)M (Bmax=12810 sites per cell) and 8.931 x 10(-8)M (Bmax=119700 sites per cell) respectively. In the presence of AFP (20 mg/L), the content of cAMP and activities of PKA were significantly elevated . The level of K-ras P21 protein was upregulated by AFP at the concentration of 20 mg/L. The monoclonal antibody against AFP could reverse the effects of AFP on the cAMP content, PKA activity and the expression of K-ras p21 gene. CONCLUSION: The effect of AFP on the cell proliferation was achieved by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 gene.
基金the National Natural Science Foundation of China,No.39870850
文摘AIM: To clone the cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) into a tetracycline-regulated gene expression system, the RevTet-On system, transduce expression vectors into a gastric carcinoma cell line-NCI-N87 and examine the effects of controlled expression of TRAIL in vitro on the gastric carcinoma cells. METHODS: The full-length cDNA of TRAIL was inserted into a vector under the control of the tetracycline-responsive element (TRE) to obtain the plasmid pRevTRE-TRAIL, which was transfected into a packaging cell line PT67. In addition, vector pRev-Tet On and pRevTRE were also transfected into PT67 separately. After hygromycin and G418 selection, the viral titer was determined. The medium containing retroviral vectors was collected and used to transduce a gastric carcinoma cell line NCI-N87. The resulting cell line NCI-N87-Tet On TRE-TRAIL and a control cell line, NCI-N87 Tet On-TRE, were established. TRAIL expression in the cell line was induced by incubating cells with doxycycline (Dox), which is a tetracycline analogue. The killing effect on gastric carcinoma cells was analyzed after induction. RESULTS: The recombinant plasmid pRev-TRE-TRAIL was constructed. After hygromycin or G418 selection, the producer cell lines PT67-TRE, PT67-TRE-TRAIL and PT67-Tet On were obtained,with titers of about 10(8)CFU.L(-1). By transducing NCI-N87 cells with retroviral vectors from these cell lines, stable cell lines NCI-N87-Tet-On TRE-TRAIL (NN3T) and control cell line NCI-N87-Tet-On-TRE (NN2T) were established. The growth curves of the selected cell lines were the same with the wild type NCI-N87. When Dox was added, cell death was obvious in the test groups (29%-77%), whereas no difference was observed in control and wild type cell lines. With the addition of a medium from the test group, human leukemia cell line Jurkat was activated till death (83%), indicating the secretion of active TRAIL proteins from the test cells to the medium. CONCLUSION: With the use of the RevTet-On system, a regulated expression system for TRAIL was constructed. Using this system, the selected killing effect of TRAIL on gastric carcinoma cell line NCI-N87 could be observed.
基金This project was supported by the National Social Science Foundation of China.
文摘The paper makes an empirical analysis on the main factors affecting IT corporate growth-human resource and R＆D. By using data of China＇s listed IT companies, we focus on the relationship among corporate value （net return of equity, ROE） or development of COl＇porate value （Tobin＇s Q） and manager＇s salary, R＆D fee, R＆D employee etc. The conclusion shows that development of corporate value （Tobin＇s Q） has correlativity positive with the plurality of board chairman and general manager, independent directors＇ proportion, R＆D fee, and R＆D employees while it is non-obvious positive with salary of superior managers, holding stocks of general manager, capital investment, and negative with corporate scale. The conclusion is accordant with the development status of Chinese IT corporate.
文摘Nowadays,the world belongs to the era of the economy full of information. The development of human resources in the entel-prise also shows the increasingly important. At present, commercial banks are not only to recruitment, introduction of outstanding professionals to enrich their team, but also the more important is potental of the existing management and staff training. Therefore, staff training and development will highlight out from the commercial bank management, which can make sure that a business adapt to the environment, realizing the fundamental value and the main enterprises for sustainable development.
