Objectives: Several studies have established the relevance of S- 100 in blood as a marker of brain damage after traumatic brain injury. However, a more specific marker is required and glial fibrillary acidic protein (...Objectives: Several studies have established the relevance of S- 100 in blood as a marker of brain damage after traumatic brain injury. However, a more specific marker is required and glial fibrillary acidic protein (GFAP) is considered to be a good candidate. Methods: In order to assess the increase of GFAP in serum (s- GFAP) after a severe traumatic brain injury (TBI) we collected daily serum samples from 59 patients with severe TBI starting on the day of the trauma. S- GFAP was measured using a sandwich ELISA. The Glasgow outcome scale (GOS) assessed outcome after 1 year. Results: All but one patient had maximal s-GFAP values above the laboratory reference value (median increased 10- fold). The highest detected levels were seen during the first days after TBI and then decreased gradually. Patients with unfavourable outcome had significantly (p < 0.001) higher maximal s-GFAP values in the acute phase compared with patients with favourable outcome. All patients (n = 5) with s-GFAP > 15.04 μ g /L died (reference level < 0.15 μ g/L). We found no significant difference in the maximal s-GFAP levels of patients with isolated brain injury in comparison with patients with multiple traumas. Conclusion: Serum-GFAP is increased during the first days after a severe traumatic brain injury and re-lated to clinical outcome.展开更多
Objective To explore the regulating effect of moxibustion on the enteric nervous system of rats with Crohn＇s disease. Methods Ten SD rats were selected randomly from 40 rats as normal control（group A）, and the othe...Objective To explore the regulating effect of moxibustion on the enteric nervous system of rats with Crohn＇s disease. Methods Ten SD rats were selected randomly from 40 rats as normal control（group A）, and the other 30 rats were established into Crohn＇s disease rat models by adopting clysis method with TNBS. On the basis of modeling successfully, the model rats were randomly divided into model group（group B）, herbs-partitioned moxibustion group（group C） and mild moxibustion group（group D） with 8 rats in each group（4 rats were dead during modeling. After modeling, 2 rats were selected from group A, and 2 rats were selected from models for determination,at last, 8 rats were included in each group）. In group C and group D, herbs-partitioned moxibustion or mild moxibustion was applied on ＂Tiānshū（天枢 ST 25）＂ bilaterally, and the rats in group A and group B were fixed as in treatment groups. HE stain was conducted, and morphological observation was performed on the colonic tissue of rats; the expression levels of proteins of S-100, SP, NPY and their receptors were observed by adopting immunohistochemical method. Results Compared with group A, the expression levels of proteins of S-100, SP and its receptor NK1R, NPY and its receptors NPY1R and NPY2R in the intestinal tract of rats in model groups increased obviously, and the differences were statistically significant.（P_（S-100）0.01, P_（SP）0.05, P_（NK1R）0.01, P_（NPY）0.05, P_（NPY1R）0.05, P_（NPY2R）0.01）, after treatment with drug-paste interposed moxibustion and mild moxibustion, the levels reduced significantly（P_（S-100）0.05, P_（SP）0.05, P_（NK1R）0.01, P_（NPY）0.05, P_（NPY1R）0.05, P_（NPY2R）0.01）. Conclusion Moxibustion treatment may regulate the expression levels of proteins of S-100, SP, NK1R, NPY, NPY1R and NPY2R through warm stimulation, alleviate inflammatory response of colonic tissue, and repair impaired colonic mucosa, thus achieving the goal of treating Crohn＇s disease.展开更多
文摘Objectives: Several studies have established the relevance of S- 100 in blood as a marker of brain damage after traumatic brain injury. However, a more specific marker is required and glial fibrillary acidic protein (GFAP) is considered to be a good candidate. Methods: In order to assess the increase of GFAP in serum (s- GFAP) after a severe traumatic brain injury (TBI) we collected daily serum samples from 59 patients with severe TBI starting on the day of the trauma. S- GFAP was measured using a sandwich ELISA. The Glasgow outcome scale (GOS) assessed outcome after 1 year. Results: All but one patient had maximal s-GFAP values above the laboratory reference value (median increased 10- fold). The highest detected levels were seen during the first days after TBI and then decreased gradually. Patients with unfavourable outcome had significantly (p < 0.001) higher maximal s-GFAP values in the acute phase compared with patients with favourable outcome. All patients (n = 5) with s-GFAP > 15.04 μ g /L died (reference level < 0.15 μ g/L). We found no significant difference in the maximal s-GFAP levels of patients with isolated brain injury in comparison with patients with multiple traumas. Conclusion: Serum-GFAP is increased during the first days after a severe traumatic brain injury and re-lated to clinical outcome.
基金Supported by:Youth Scientific Research Project of Shanghai Municipal Commission of Health and Family Planning:2013Q042National Key Basic Research Development Program(973 Program):2015CB554500+2 种基金Support Plan of New Century Excellent Talents of Ministry of Education:NCET-13-0907National Natural Science Foundation:81574079Shanghai Municipal Youth Science and Technology Venus Project:16QA1403400
文摘Objective To explore the regulating effect of moxibustion on the enteric nervous system of rats with Crohn＇s disease. Methods Ten SD rats were selected randomly from 40 rats as normal control（group A）, and the other 30 rats were established into Crohn＇s disease rat models by adopting clysis method with TNBS. On the basis of modeling successfully, the model rats were randomly divided into model group（group B）, herbs-partitioned moxibustion group（group C） and mild moxibustion group（group D） with 8 rats in each group（4 rats were dead during modeling. After modeling, 2 rats were selected from group A, and 2 rats were selected from models for determination,at last, 8 rats were included in each group）. In group C and group D, herbs-partitioned moxibustion or mild moxibustion was applied on ＂Tiānshū（天枢 ST 25）＂ bilaterally, and the rats in group A and group B were fixed as in treatment groups. HE stain was conducted, and morphological observation was performed on the colonic tissue of rats; the expression levels of proteins of S-100, SP, NPY and their receptors were observed by adopting immunohistochemical method. Results Compared with group A, the expression levels of proteins of S-100, SP and its receptor NK1R, NPY and its receptors NPY1R and NPY2R in the intestinal tract of rats in model groups increased obviously, and the differences were statistically significant.（P_（S-100）0.01, P_（SP）0.05, P_（NK1R）0.01, P_（NPY）0.05, P_（NPY1R）0.05, P_（NPY2R）0.01）, after treatment with drug-paste interposed moxibustion and mild moxibustion, the levels reduced significantly（P_（S-100）0.05, P_（SP）0.05, P_（NK1R）0.01, P_（NPY）0.05, P_（NPY1R）0.05, P_（NPY2R）0.01）. Conclusion Moxibustion treatment may regulate the expression levels of proteins of S-100, SP, NK1R, NPY, NPY1R and NPY2R through warm stimulation, alleviate inflammatory response of colonic tissue, and repair impaired colonic mucosa, thus achieving the goal of treating Crohn＇s disease.
