AIM:To define the topography of mast cells and their numbers in cases of Hirschsprung's disease(HD)and non-HD,assess neural hypertrophy using imaging software and to study the relationship between mast cells and n...AIM:To define the topography of mast cells and their numbers in cases of Hirschsprung's disease(HD)and non-HD,assess neural hypertrophy using imaging software and to study the relationship between mast cells and nerve fibers.METHODS:HE stained sections of 32 cases of chronic constipation in the age group of 0-14 years were reviewed for ganglion cells.AChE staining was performed on frozen sections of colonic and rectal biopsies.Based on their findings cases were divided into HD and non-HD and mast cells stained by toluidine blue were evaluated.Image analysis by computerized software was applied to S-100 stained sections for assessment of neural hypertrophy.RESULTS:Difference between number of mast cells in HD group(mean=36.44)and in non-HD group(mean =14.79)was statistically significant.Image analysis morphometry on S-100 stained sections served as a useful adjunct.The difference between number,size,and perimeter of the nerve fibers between HD and non-HD group was statistically significant.CONCLUSION:Mast cells are significantly increased in HD and their base line values are much higher in Indian children than that reported in Western literature.Their role in HD needs further research.Morphometry of S-100 stained nerve fibers is a useful adjunct to conventional methods for diagnosis of HD.展开更多
The anterior pituitary gland regulates growth,metabolism,and reproduction by secreting hormones.Folliculo-stellate cells(FSC)are non-endocrine cells located among hormone-producing cells in the anterior pituitary glan...The anterior pituitary gland regulates growth,metabolism,and reproduction by secreting hormones.Folliculo-stellate cells(FSC)are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands,but little is known about the exact roles of those cells.Although,with their net organization,they seem to have an important role in the hormonal cells regulation and maintenance.In this work,the first ever made in this area,33 pituitaries of three groups of mice(18 wild type[WT],11 genetically obese[OB]and 4 under a high-fat diet[HFD])were studied in order to determine if there was any relation between the number of FSC and alterations of the basal metabolism in each group of mice.For that,immunohistochemical staining using the S-100 protein was used and also the Image-J software,to calculate the percentage of FSC present in each sample.The authors found that,although there wasn’t any significant difference between WT and OB mice,the group of HFD mice tend to have substantially higher percentage of FSC than the mice from other groups.This might suggest some yet unknown link between diet,precisely with a high-fat diet,and the presentation of FSC in the anterior pituitary.展开更多
Malignant Gastrointestinal Neuroectodermal Tumor (GNET) is a recently described rare malignant neoplasm, resembling “clear cell sarcoma of tendons and aponeuroses” morphologically, immunohistochemically and molecula...Malignant Gastrointestinal Neuroectodermal Tumor (GNET) is a recently described rare malignant neoplasm, resembling “clear cell sarcoma of tendons and aponeuroses” morphologically, immunohistochemically and molecular pathologically. We will describe a case of soft tissue sarcoma located at right cervical region of a 9-year-old male patient, mimicking malignant GNET, which can be considered as extragastrointestinal counterpart of this tumor. These tumors have poor prognosis. This is unique, possibly the first case of a soft tissue sarcoma resembling GNET.展开更多
文摘AIM:To define the topography of mast cells and their numbers in cases of Hirschsprung's disease(HD)and non-HD,assess neural hypertrophy using imaging software and to study the relationship between mast cells and nerve fibers.METHODS:HE stained sections of 32 cases of chronic constipation in the age group of 0-14 years were reviewed for ganglion cells.AChE staining was performed on frozen sections of colonic and rectal biopsies.Based on their findings cases were divided into HD and non-HD and mast cells stained by toluidine blue were evaluated.Image analysis by computerized software was applied to S-100 stained sections for assessment of neural hypertrophy.RESULTS:Difference between number of mast cells in HD group(mean=36.44)and in non-HD group(mean =14.79)was statistically significant.Image analysis morphometry on S-100 stained sections served as a useful adjunct.The difference between number,size,and perimeter of the nerve fibers between HD and non-HD group was statistically significant.CONCLUSION:Mast cells are significantly increased in HD and their base line values are much higher in Indian children than that reported in Western literature.Their role in HD needs further research.Morphometry of S-100 stained nerve fibers is a useful adjunct to conventional methods for diagnosis of HD.
文摘The anterior pituitary gland regulates growth,metabolism,and reproduction by secreting hormones.Folliculo-stellate cells(FSC)are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands,but little is known about the exact roles of those cells.Although,with their net organization,they seem to have an important role in the hormonal cells regulation and maintenance.In this work,the first ever made in this area,33 pituitaries of three groups of mice(18 wild type[WT],11 genetically obese[OB]and 4 under a high-fat diet[HFD])were studied in order to determine if there was any relation between the number of FSC and alterations of the basal metabolism in each group of mice.For that,immunohistochemical staining using the S-100 protein was used and also the Image-J software,to calculate the percentage of FSC present in each sample.The authors found that,although there wasn’t any significant difference between WT and OB mice,the group of HFD mice tend to have substantially higher percentage of FSC than the mice from other groups.This might suggest some yet unknown link between diet,precisely with a high-fat diet,and the presentation of FSC in the anterior pituitary.
文摘Malignant Gastrointestinal Neuroectodermal Tumor (GNET) is a recently described rare malignant neoplasm, resembling “clear cell sarcoma of tendons and aponeuroses” morphologically, immunohistochemically and molecular pathologically. We will describe a case of soft tissue sarcoma located at right cervical region of a 9-year-old male patient, mimicking malignant GNET, which can be considered as extragastrointestinal counterpart of this tumor. These tumors have poor prognosis. This is unique, possibly the first case of a soft tissue sarcoma resembling GNET.
