Netrin-1 signaling pathway mechanisms in neurodegenerative diseases

Netrin-1 and its receptors play crucial roles in inducing axonal growth and neuronal migration during neuronal development.Their profound impacts then extend into adulthood to encompass the maintenance of neuronal survival and synaptic function.Increasing amounts of evidence highlight several key points:(1)Diminished Netrin-1 levels exacerbate pathological progression in animal models of Alzheimer’s disease and Parkinson’s disease,and potentially,similar alterations occur in humans.(2)Genetic mutations of Netrin-1 receptors increase an individuals’susceptibility to neurodegenerative disorders.(3)Therapeutic approaches targeting Netrin-1 and its receptors offer the benefits of enhancing memory and motor function.(4)Netrin-1 and its receptors show genetic and epigenetic alterations in a variety of cancers.These findings provide compelling evidence that Netrin-1 and its receptors are crucial targets in neurodegenerative diseases.Through a comprehensive review of Netrin-1 signaling pathways,our objective is to uncover potential therapeutic avenues for neurodegenerative disorders.

Gut microbiota dysbiosis contributes toα-synuclein-related pathology associated with C/EBPβ/AEP signaling activation in a mouse model of Parkinson’s disease

Parkinson’s disease is a neurodegenerative disease characterized by motor and gastrointestinal dysfunction.Gastrointestinal dysfunction can precede the onset of motor symptoms by several years.Gut microbiota dysbiosis is involved in the pathogenesis of Parkinson’s disease,whether it plays a causal role in motor dysfunction,and the mechanism underlying this potential effect,remain unknown.CCAAT/enhancer binding proteinβ/asparagine endopeptidase(C/EBPβ/AEP)signaling,activated by bacterial endotoxin,can promoteα-synuclein transcription,thereby contributing to Parkinson’s disease pathology.In this study,we aimed to investigate the role of the gut microbiota in C/EBPβ/AEP signaling,α-synuclein-related pathology,and motor symptoms using a rotenone-induced mouse model of Parkinson’s disease combined with antibiotic-induced microbiome depletion and fecal microbiota transplantation.We found that rotenone administration resulted in gut microbiota dysbiosis and perturbation of the intestinal barrier,as well as activation of the C/EBP/AEP pathway,α-synuclein aggregation,and tyrosine hydroxylase-positive neuron loss in the substantia nigra in mice with motor deficits.However,treatment with rotenone did not have any of these adverse effects in mice whose gut microbiota was depleted by pretreatment with antibiotics.Importantly,we found that transplanting gut microbiota derived from mice treated with rotenone induced motor deficits,intestinal inflammation,and endotoxemia.Transplantation of fecal microbiota from healthy control mice alleviated rotenone-induced motor deficits,intestinal inflammation,endotoxemia,and intestinal barrier impairment.These results highlight the vital role that gut microbiota dysbiosis plays in inducing motor deficits,C/EBPβ/AEP signaling activation,andα-synuclein-related pathology in a rotenone-induced mouse model of Parkinson’s disease.Additionally,our findings suggest that supplementing with healthy microbiota may be a safe and effective treatment that could help ameliorate the progression of motor deficits in patients with Parkinson’s disease.

Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding

Zooplankton,a crucial component of urban wetland,are one of the effective bioindicators for monitoring the feeding stocks of organisms at higher trophic levels and assessing the ecological quality of ecosystems.However,information about the characteristics of epiphytic zooplankton community structure resulted from traditional methods is limited and hindered by the large amount of detritus and sludge attached to the macrophytes.We investigated the epiphytic zooplankton communities associated with macrophytes(Vallisneria,Nymphaea,and Thalia dealbata)in a subtropical wetland using as DNA markers of the 18 S rRNA gene and the mitochondrial cytochrome c oxidase subunit I(COI)gene.A total of 241 OTUs of zooplankton were obtained from COI amplicons,including 194 OTUs of Rotifera,22 of Cladocera,and 25 of Copepoda,while only 62 OTUs of zooplankton were obtained from 18 S rDNA amplicons including 34 OTUs of Rotifera and 28 of Copepoda.The zooplankton communities associated with the three macrophytes were similar,but they differed significantly from those in the open waters.However,there were no significant temporal differences among the zooplankton communities.Epiphytic zooplankton communities were dominated by littoral zooplankton such as Testudinella,Lecane,and Philodina.Microzooplankton,especially littoral species,utilize macrophytes as food sources and as refuges against predation.This further led to an increase inαandβdiversity of zooplankton communities in urban wetlands.Our result suggests that the joint use of multiple molecular markers could improve the taxonomic resolution and generate a comprehensive biodiversity profile of zooplankton.

Prognostic significance and relationship of SMAD3 phosphoisoforms and VEGFR-1 in gastric cancer:A clinicopathological study

BACKGROUND The TGF-β/SMAD3 and VEGFR-1 signaling pathways play important roles in gastric cancer metastasis.SMAD3 phosphorylation is a crucial prognostic marker in gastric cancer.AIM To determine the prognostic value and relationship of SMAD3 phospho-isoforms and VEGFR-1 in gastric cancer.METHODS This was a single-center observational study which enrolled 98 gastric cancer patients and 82 adjacent normal gastric tissues from patients aged 32-84 years(median age 65)between July 2006 and April 2007.Patients were followed up until death or the study ended(median follow-up duration of 28.5 mo).The samples were used to generate tissue microarrays(TMAs)for immunohistochemical(IHC)staining.The expressions of TGF-β1,pSMAD3C(S423/425),pSMAD3L(S204),and VEGFR-1 in gastric cancer(GC)tumor tissue and normal tissue were measured by IHC staining using TMAs obtained from 98 GC patients.Prognosis and survival information of the patients was recorded by Outdo Biotech from May 2007 to July 2015.The relationship between TGF-β1,pSMAD3C(S423/425),pSMAD3L(S204),and VEGFR-1 protein expression levels was analyzed using Pearson's correlation coefficient.The relationship between protein expression levels and clinicopathological parameters was analyzed using the Chi-squared test.A survival curve was generated using the Kaplan-Meier survival analysis.RESULTS TGFβ-1 and VEGFR-1 expression was significantly upregulated in gastric cancer tissue compared to adjacent noncancerous tissue.The positive expression of phosphorylated isoforms of Smad3 varied depending on the phosphorylation site[pSMAD3C(S423/425):51.0%and pSMAD3L(S204):31.6%].High expression of pSMAD-3L(S204)was significantly correlated with larger tumors(P=0.038)and later N stages(P=0.035).Additionally,high expression of VEGFR-1 was closely correlated with tumor size(P=0.015)and pathological grading(P=0.013).High expression of both pSMAD3L(S204)and VEGFR-1 was associated with unfavorable outcomes in terms of overall survival(OS).Multivariate analysis indicated that high expression of pSMAD3L(S204)and VEGFR-1 were independent risk factors for prognosis in GC patients.VEGFR-1 protein expression was correlated with TGF-β1(r=0.220,P=0.029),pSMAD3C(S423/425)(r=0.302,P=0.002),and pSMAD3L(S204)(r=0.201,P=0.047),respectively.Simultaneous overexpression of pSMAD3L(S204)and VEGFR-1 was associated with poor OS in gastric cancer patients.CONCLUSION Co-upregulation of pSMAD3L(S204)and VEGFR-1 can serve as a predictive marker for poor gastric cancer prognosis,and pSMAD3L(204)may be involved in enhanced gastric cancer metastasis in a VEGFR-1-dependent manner.

Pancreatic surgery and tertiary pancreatitis services warrant provision for support from a specialist diabetes team

Pancreatic surgery units undertake several complex operations,albeit with consi-derable morbidity and mortality,as is the case for the management of complicated acute pancreatitis or chronic pancreatitis.The centralisation of pancreatic surgery services,with the development of designated large-volume centres,has contribu-ted to significantly improved outcomes.In this editorial,we discuss the complex associations between diabetes mellitus(DM)and pancreatic/periampullary disease in the context of pancreatic surgery and overall management of complex pancreatitis,highlighting the consequential needs and the indispensable role of specialist diabetes teams in support of tertiary pancreatic services.Type 3c pan-creatogenic DM,refers to DM developing in the setting of exocrine pancreatic disease,and its identification and management can be challenging,while the glycaemic control of such patients may affect their course of treatment and outcome.Adequate preoperative diabetes assessment is warranted to aid identification of patients who are likely to need commencement or escalation of glucose lowering therapy in the postoperative period.The incidence of new onset diabetes after pancreatic resection is widely variable in the literature,and depends on the type and extent of pancreatic resection,as is the case with pancreatic parenchymal loss in the context of severe pancreatitis.Early involvement of a specialist diabetes team is essential to ensure a holistic management.In the current era,large volume pancreatic surgery services commonly abide by the principles of enhanced recovery after surgery,with inclusion of provisions for optimisation of the perioperative glycaemic control,to improve outcomes.While various guidelines are available to aid perioperative management of DM,auditing and quality improvement platforms have highlighted deficiencies in the perioperative management of diabetic patients and areas of required improvement.The need for perioperative support of diabetic patients by specialist diabetes teams is uniformly underlined,a fact that becomes clearly more prominent at all different stages in the setting of pancreatic surgery and the management of complex pancreatitis.Therefore,pancreatic surgery and tertiary pancreatitis services must be designed with a provision for support from specialist diabetes teams.With the ongoing accumulation of evidence,it would be reasonable to consider the design of specific guidelines for the glycaemic management of these patients.

State of the HIV, Hepatitis B and C Virus Pandemic from 2003 to 2022 in Burkina Faso: Evolution of Prevalence Trends and Strategic Recommendations to Achieve the WHO’s Goal for Their Eradication by 2030

Background: The World Health Organization (WHO) has set a goal to eradicate or at least significantly reduce the prevalence the human immunodeficiency virus (HIV), hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) by 2030. The main objective was to provide an evolving overview of the prevalence of HIV, HBV and HCV infection between 2003 and 2022 in Burkina Faso. Methods: It was a retrospective cross-sectional study based on data from 2003 to 2022. The data were collected using information available in the databases of the HOSCO and CERBA laboratories and included all individuals who underwent HIV and/or HBV and/or HCV testing. Data analysis was performed using SPSS version 20.0, EpiInfo 7, and R version 4.1.0. Results were considered statistically significant if p Results: The study recorded 7432 samples and the mean age of the subjects was 27.98 ± 8.50 years. During this period, the respective prevalence of HIV, HBV, and HCV were 4.66% (346/7432), 8.77% (582/6636) and 5.54% (322/5816). However, from 2003 to 2022, there was a significant decrease (P y=−1.75x+12.59;y=−0.24x+10.01and y=−0.11x+6.02, with “y” corresponding to prevalence and “x” to the years. Conclusion: Burkina Faso needs to rigorously apply prevention and control strategies recommended by the WHO by 2030.

基于S7-300PLC的净水厂V型滤池控制系统设计思路探究

净水厂是城市供水的主要来源,净水厂出厂水质的安全可靠是确保安全饮用水的首要任务。滤池是净水厂工艺流程中水处理关键环节,对出厂水质产生直接影响。随着科技的发展和进步,实现净水厂滤池自动化高效运行,本文围绕基于S7-300 PLC的净水厂V型滤池控制系统设计展开一系列的探讨,分析研究了净水厂V型滤池在正常过滤过程和反冲洗功能的难点和解决方法,并实现净水厂V型滤池在正常过滤过程和反冲洗功能控制系统精确控制,经过三亚市某水厂的实际运行检验,系统可靠稳定,同时使得净水厂出厂水质符合生活饮用水标准,希望能给净水厂V型滤池控制系统设计上带来一些帮助。

多奈哌齐联合丁苯酞治疗老年阿尔茨海默病的效果及对患者Hcy、CysC、VEGF的影响

目的观察多奈哌齐联合丁苯酞治疗老年阿尔茨海默病(AD)的效果及对患者血清同型半胱氨酸(Hcy)、胱抑素C(CysC)、血管内皮生长因子(VEGF)的影响。方法选取2021年1月—2022年6月福州市中医院收治的老年AD患者90例作为观察组,选择同期进行健康体检的90例健康老年人作为健康对照组,将观察组患者按随机数字表法分为Ⅰ亚组、Ⅱ亚组、Ⅲ亚组各30例,分别给予多奈哌齐、丁苯酞及多奈哌齐+丁苯酞治疗,3组均治疗24周。治疗前、治疗24周结束时对2组受试者进行血清Hcy、CysC、VEGF检测及记录简易精神状态检查量表(MMSE)、日常生活活动能力量表(ADL)评分,根据MMSE评分将患者分为轻度、中度、重度,比较不同病情程度患者治疗前血清Hcy、CysC、VEGF水平,采用Pearson相关分析探讨Hcy、CysC、VEGF与病情程度的相关性,治疗24周结束时评价临床疗效。结果观察组血清Hcy、CysC水平明显高于健康对照组,血清VEGF水平明显低于健康对照组(P均<0.01)。轻度、中度、重度患者血清Hcy、CysC、VEGF水平比较,差异有统计学意义(F/P=20.917/<0.001、37.397/<0.001、60.507/<0.001);Pearson相关分析显示,Hcy、CysC与病情程度呈正相关(r=0.853、0.864,P<0.01),VEGF与病情程度呈负相关(r=-0.901,P<0.01)。3亚组治疗总有效率比较,差异有统计学意义(χ^(2)=6.477,P=0.039);Ⅲ亚组治疗总有效率高于Ⅰ亚组、Ⅱ亚组(χ^(2)/P=5.963/0.015、4.812/0.028),但Ⅰ亚组、Ⅱ亚组治疗总有效率比较,差异无统计学意义(P>0.05)。3亚组治疗24周后MMSE评分与血清VEGF水平较治疗前升高,ADL评分与血清Hcy、CysC水平较治疗前降低,且治疗后3亚组间MMSE、ADL评分与血清Hcy、CysC、VEGF水平比较,差异有统计学意义(P<0.01),Ⅲ亚组治疗后相应指标与Ⅰ亚组、Ⅱ亚组比较差异有统计学意义(P<0.01),但Ⅰ亚组、Ⅱ亚组比较差异无统计学意义(P>0.05)。结论Hcy、CysC、VEGF参与老年AD的形成过程,Hcy、CysC与病情程度呈正相关,VEGF与病情程度呈负相关,多奈哌齐联合丁苯酞治疗老年AD能更有效降低Hcy、CysC水平,升高VEGF水平,进而提高临床治疗效果。

cyclin E、p53、CD44s、CD44v6与早期乳腺癌的相关性及预后预测分析

目的分析肿瘤组织的细胞周期素E(cyclin E)和p53、CD44s、CD44v6蛋白与早期乳腺癌的相关性及预后预测价值。方法选取2018年7月—2020年7月喀什地区第二人民医院收治的80例临床分期为Ⅰ~Ⅱ期的早期乳腺癌患者进行回顾性分析,将其分为恶性组;另选取同期喀什地区第二人民医院收治的80例通过病理学检验确诊为良性乳腺肿瘤患者,将其分为良性组。分别对两组患者留存的肿瘤组织进行免疫组化检测,检测cyclin E、p53、CD44s、CD44v6表达,并对比上述指标的阳性率,应用Spearman相关分析方法分析cyclin E、p53、CD44s、CD44v6与早期乳腺癌的相关性。所有患者均采取手术治疗,随后对80例早期乳腺癌患者进行3年随访,依照随访结果将患者分为2个亚组,即预后不良组(n=25)和预后良好组(n=55),对比两组患者一般临床特征及cyclin E、p53、CD44s、CD44v6阳性率,并应用Logistic回归分析cyclin E、p53、CD44s、CD44v6对早期乳腺癌预后的预测价值。结果恶性组患者cyclin E、p53、CD44s、CD44v6阳性率分别为42.50%、61.25%、77.50%、81.25%,明显高于良性组1.25%、5.00%、10.00%、12.50%(P<0.05);Spearman相关分析结果显示:cyclin E、p53、CD44s、CD44v6与早期乳腺癌呈正相关(P<0.05);预后良好组与预后不良组患者绝经情况、年龄、病理类型、治疗方式、三阴性乳腺癌、肿瘤大小、组织分化程度对比,差异无统计学意义(P>0.05),预后良好组与预后不良组患者临床分期、cyclin E、p53、CD44s、CD44v6阳性例数对比,差异有统计学意义(P<0.05);Logistic回归分析结果表明:cyclin E、p53、CD44s、CD44v6阳性表达为影响早期乳腺癌预后的独立影响因素(P<0.05)。结论cyclin E、p53、CD44s、CD44v6表达水平与乳腺癌的发生、发展具有明显关系,且上述指标呈阳性表达为早期乳腺癌预后不良的独立预测因素。

Evaluation of Technical Education by Using a Modern Structured MOODLE Laboratory Course, in Relation to Recent Data

E-learning platforms support education systems worldwide, transferring theoretical knowledge as well as soft skills. In the present study high-school pupils’, and adult students’ opinions were evaluated through a modern structured MOODLE interactive course, designed for the needs of the laboratory course “Automotive Systems”. The study concerns Greek secondary vocational education pupils aged 18 and vocational training adult students aged 20 to 50 years. The multistage, equal size simple random cluster sample was used as a sampling method. Pupils and adult students of each cluster completed structured 10-question questionnaires both before and after attending the course. A total of 120 questionnaires were collected. In general, our findings disclosed that the majority of pupils and adult students had significantly improved their knowledge and skills from using MOODLE. They reported strengthening conventional teaching, using the new MOODLE technology. The satisfaction indices improved quite, with the differences in their mean values being statistically significant.

基于注意力机制的轻量化YOLO v5s蓝莓检测算法

为实现自然环境下蓝莓的精确快速检测,在YOLO v5s的基础上提出了一种结合轻量级网络和注意力机制的改进算法。首先,在主干网络和检测头的位置去除了最大目标检测层的结构,因而降低模型的参数量,增强模型对小目标的检测能力。其次,将MHSA(Multi‐head self‐attention,多头自注意力)替换了SPPF(Spatial pyramid pooling‐fast,快速空间金字塔池化)前面的C3模块,使模型学习到更全面的特征表示,增强模型对蓝莓图像中复杂空间关系和上下文信息的理解能力。最后,在C3模块中加入了S-PSA(Sequential polarized self‐attention,顺序极化自注意力),以便模型能够更好地捕捉特征图中相邻区域之间的上下文依赖关系。结果表明,改进后的YOLO v5s算法对成熟、半成熟和未成熟蓝莓的检测精度分别提升1.2、4.4、2.6百分点,平均精度提升2.7百分点,模型参数量减少76.0%。与当前主流轻量化目标检测模型相比,改进后的模型性能更加优越,能为自然环境下蓝莓采摘机器人视觉系统提供一种有效的方案。

基于C/S架构和物联网技术的可移动医疗设备共享调配系统的设计与应用

目的 设计一个可移动医疗设备高效管理与临床科室共享调配相结合的医疗设备共享管理平台,实现可移动医疗设备使用的全过程管理。方法 基于C/S架构,利用物联网技术优化整合现有医疗设备大数据资源,结合医院需求,构建可移动医疗设备共享调配系统,实现医疗设备共享管理、预约借用、统计分析等核心功能。通过比较系统上线前后呼吸机调配次数、平均调配时间、呼吸机使用率、单台呼吸机日均收入4个指标,分析系统应用效果。结果 系统上线后,呼吸机调配次数明显增加、平均调配时间明显降低,呼吸机使用率、单台呼吸机日均收入明显升高,且差异均有统计学意义(P﹤0.05),表明系统对提高呼吸机调配效率与使用效率有促进作用。结论 可移动医疗设备共享调配系统优化了医院设备管理流程,实现了医疗资源的统一调配,有效满足临床紧急抢救时医疗设备调配使用的相关需求,提高了医疗设备周转率以及设备管理人员的工作效率,有助于医院节约人员成本、降低采购费用,为医院管理者制定相关决策提供数据支撑。

妊娠糖尿病患者蛋白C、蛋白S和抗凝血酶Ⅲ水平及其与孕周的关系

目的探讨妊娠糖尿病(GDM)患者蛋白C(PC)、蛋白S(PS)和抗凝血酶Ⅲ(ATⅢ)水平及其与孕周的关系。方法选择2019年1月至2023年3月在医院就诊的232例GDM孕妇作为观察组,同时随机选取同期在医院进行孕检的268例正常孕妇作为对照组,比较两组一般资料和PC、PS和ATⅢ水平;按孕周将观察组和对照组分为孕中期和孕晚期,分别比较孕中期和孕晚期观察组和对照组PC、PS和ATⅢ水平。通过相关性分析,分析PC、PS和ATⅢ水平与孕周的相关性。结果与对照组相比,观察组PS和PC水平降低(P<0.05),但ATⅢ水平差异无统计学意义(P>0.05)。GDM患者PC和ATⅢ水平与孕周呈负相关(r=-0.156、-0.134,P<0.05)。结论GDM患者PS和PC水平降低,且随孕周增大,PC、PS和ATⅢ水平降低,应尽早进行干预,以减少血栓形成的风险。

基于轻量化YOLO v8s-GD的自然环境下百香果快速检测模型

为了提高百香果检测精度,并将深度学习模型部署在移动平台上,实现快速实时推理,本文提出一种基于改进YOLO v8s的轻量化百香果检测模型(YOLO v8s-GD)。使用聚集和分发机制(GD)替换颈部特征融合网络,提高模型对百香果图像特征信息跨层融合能力和模型泛化能力;通过基于层自适应幅度的剪枝(LAMP)修剪模型,损失一定精度换取减小模型体积,减少模型参数量,以实现在嵌入式设备上快速检测;运用知识蒸馏学习策略弥补因剪枝而损失的检测精度,提高模型检测性能。实验结果表明,对于自然环境下采集的百香果数据集,改进后模型参数量和内存占用量相比原YOLO v8s基线模型分别降低63.88%和62.10%,精确率(Precision)和平均精度(AP)相较于原模型分别提高0.9、2.3个百分点,优于其他对比模型。在Jetson Nano和Jetson Tx2嵌入式设备上实时检测帧率(FPS)分别为5.78、19.38 f/s,为原模型的1.93、1.24倍。因此,本文提出的改进后模型能够有效检测复杂环境下百香果目标,为实际场景中百香果自动采摘等移动端检测设备部署和应用提供理论和技术支持。

基于YOLOv5s的钢铁表面缺陷检测算法

为改善因小目标检测效果差而导致的钢铁表面缺陷检测精度差的问题,以YOLOv5s为基础,通过在主干网络添加注意力机制(attention mechanism,SE),将C2f模块代替C3模块,将双向特征金字塔网络(bidirectional feature pyramid network,BIFPN)网络代替颈部网络中的路径聚合网络(path aggregation network,PAN)网络的这三种方法来提升模型对缺陷小目标的检测能力。旨在提升检测精度并达到实时检测要求。结果表明,改进后的YOLOv5s-SCB算法在NEU-DET(northeastern university-detect)上的均值平均精度(mean average precision,mAP)值达到77.9%,在达到实时检测的前提下,相较于YOLOv5s网络提高了3.7%,与其余基于YOLOv5s改进的算法及YOLOv8相比,YOLOv5s-SCB实现了更好的检测效果。可见本文提出的钢铁表面缺陷检测算法YOLOv5S-SCB可以更好地完成钢铁表面缺陷检测。

NOTCH3基因C260S位点突变导致CADASIL的临床和影像学特征分析

目的探讨NOTCH3基因第5外显子C260S位点突变导致的伴有皮层下梗死和白质脑病的常染色体显性遗传性脑动脉病(cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy,CADASIL)家系的临床和影像学特征。方法选取2021年12月首都医科大学附属北京同仁医院来自同一家庭的CADASIL患者,对所有患者进行NOTCH3基因测序,回顾性分析患者的临床表现和头颅影像学特征。复习既往文献报道的导致同一位置氨基酸改变的其他突变类型的临床及影像学特征。结果4名家庭成员中,包括先证者(46岁,女)及其两个姐姐(分别为48岁和50岁)和女儿(18岁)。先证者及其父亲、两个姐姐都有偏头痛病史,其中大姐有记忆力减退;先证者患有脑梗死及伴有视觉先兆的偏头痛;先证者女儿体健;先证者父亲因脑梗死去世。4名家庭成员均存在C260S位点的NOTCH3基因突变。既往文献无此位点突变的报道,先证者头颅MRI示右侧脑桥亚急性梗死,颞叶、脑室周围及脑干异常高信号改变,其大姐脑桥可见腔隙性梗死灶。结论NOTCH3基因第5外显子c.778T>A(p.C260S)的罕见突变导致的CADASIL发病时间早,早期会出现认知障碍。合并偏头痛的脑干梗死患者,需警惕CADASIL的可能。

miR29c下调S100A4蛋白表达参与人胃癌细胞增殖促进凋亡

目的探讨miR-29c对人胃腺癌细胞增殖、凋亡的影响及其与转移相关蛋白S100A4的关系。方法采用细胞培养人胃癌细胞系BGC-823、SGC-7901和人正常胃粘膜上皮GES-1细胞,应用qRT-PCR检测各株细胞miR29c的表达。用hsamiR29c-mimics类似物(miR29c组),hsa-miR29c-inhibitor(阴性对照组)分别转染BGC-823细胞,空白对照组加入转染液,用CCK8及MTT法检测细胞增殖,流式细胞术检测细胞凋亡,Western blot检测S100A4相关肿瘤相关蛋白的表达。结果与正常胃粘膜上皮细胞GES-1表达率(1.131±0.069)相比,MiR-29c在胃癌细胞BGC823(0.254±0.025),SGC-7901(0.466±0.057)(F=57.5,P<0.05)。BGC823细胞转染miR29c模拟物后MiR-29c的表达量为:4.126±0.231,0.405±0.174,0.557±0.102,差异具有统计学意义(F=850.1,P<0.05)。转染24h、48h、72h后,MiR-29c组细胞的OD450值分别为:1.074±0.127、1.609±0.178、2.008±0.104;阴性对照组为:0.419±0.169、0.477±0.092、0.738±0.153,空白对照组为:0.830±0.305。其中,转染48h、72h后,MiR-29c组明显高于对照组(F=59.46,P<0.05)。细胞转染24h后,miR29c组细胞凋亡率分别是(6.900±0.414)%、(4.490±0.316)%、(3.850±0.243)%,,阴性对照组凋亡率分别是(10.830±0.231)%、(11.940±0.279)%、(13.530±0.941)%,空白对照组凋亡率分别是:(10.190±0.188)%、(11.76±0.569)%、(13.260±0.987),(P<0.001).各组S100A4蛋白的表达分别为0.770±0.028、1.109±0.083、1.024±0.042,(P=0.0021)。结论miR29c在胃癌细胞系中表达降低,通过抑制S100A4抑制人胃癌细胞增殖并促进胃癌细胞凋亡。

谷氨酸、CRP及S-100β蛋白水平与脑出血患者预后的关系

目的:分析谷氨酸、C反应蛋白(CRP)及S-100β蛋白水平与脑出血患者预后的关系。方法:选择2021年1月—2023年12月南昌大学第四附属医院收治的80例脑出血患者,患者入院后采集血样,测定血清谷氨酸、CRP及S-100β蛋白水平。随访90 d,采用格拉斯哥预后量表(GPS)进行预后评价,根据GPS评分分为预后不良组(1~3分)、预后良好组(4~5分),比较两组谷氨酸、CRP及S-100β蛋白水平,分析谷氨酸、CRP及S-100β蛋白水平与预后的相关性,采用受试者操作特征(ROC)曲线分析这些生物标志物对患者预后预测的价值。结果:在80例脑出血患者中,预后不良发生率为43.75%(35/80)。预后不良组血清谷氨酸、CRP及S-100β蛋白水平均高于预后良好组(P<0.05)。点二列相关系数分析结果显示,谷氨酸、CRP及S-100β蛋白与预后呈正相关(r=0.709、0.764、0.654,P<0.05)。Pearson相关系数分析显示,谷氨酸、CRP与S-100β蛋白呈正相关(r=0.361、0.431,P<0.001)。ROC曲线显示,谷氨酸、CRP、S-100β蛋白对患者预后预测的AUC分别为0.912、0.945、0.881,有一定预测价值。结论:谷氨酸、CRP及S-100β蛋白水平与脑出血患者的预后呈正相关,并具有一定的预测价值,在评估脑出血患者的预后时,这些可作为重要的参考指标之一。

基于STC12C5A60S2单片机显示模块的实验研究

以常用的STC12C5A60S2单片机为主控芯片,以实验室单片机平台为载体,分别对数码管、LCD1602、LCD12864、OLED以及TFT五种显示屏进行DS18B20温度传感器采集与显示实验。实验测试结果表明五种显示模块均能有效采集并显示当前温度值,对比分析各种显示模块的性能,为不同应用场景下的显示需求提供了参考和借鉴,具有重要的实际应用价值。

纳米C-S-H/PCE对蒸养UHPC力学性能增强机理研究

目前超高性能混凝土(UHPC)制品的力学性能有待进一步提升,采用纳米C-S-H/PCE对UHPC进行增强在理论上是一种可行的技术途径。本文研究了蒸养条件下掺入以聚羧酸(PCE)为分散剂的纳米水化硅酸钙(C-S-H/PCE)对UHPC力学性能的影响,通过测试水化热、水化产物、微观形貌和孔结构等对其机理进行了分析。结果表明,掺入3.0%(质量分数)纳米C-S-H/PCE可以显著提高蒸养UHPC的抗压强度和抗折强度。掺入纳米C-S-H/PCE可以促进UHPC的早期水化,生成更多的水化硅酸钙(C-S-H)凝胶,使水化放热温峰增高,并缩短达到水化温峰的时间。掺入纳米C-S-H/PCE可有效改善蒸养UHPC的孔结构,在掺量为3.0%(质量分数)时总孔隙率最小,有害孔比例最低。该研究可为纳米C-S-H/PCE在蒸养UHPC中的应用提供参考。