A83-01 is a selective inhibitor of the TGF-β type I receptor ALK,which inhibits the TGF-β-induced epithelial-to-mesenchymal transition（EMT） via the inhibition of Smad2 phosphorylation.Previous studies have showed ...A83-01 is a selective inhibitor of the TGF-β type I receptor ALK,which inhibits the TGF-β-induced epithelial-to-mesenchymal transition（EMT） via the inhibition of Smad2 phosphorylation.Previous studies have showed that A83-01 promoted somatic cellular reprogramming significantly.Male germline stem cells（mGSCs）,as an alternative resource of pluripotent stem cells derived adult testis,have promising valuable in clinic medicine and regeneration,however,the derivation of mGSCs was complex and difficult.What the role A83-01 plays in promoting the proliferation of mGSCs is still unknown.In this study,combined with A83-01 and knockout serum replacement（KSR） medium,we obtained a relatively feeder-and serum-free system for mGSCs culturing in vitro and the optimal concentration of A83-01 was 0.25 μmol L-1.After continuous culturing,the proliferation efficiency of undifferentiated mGSCs and differentiation capacity of mGSC were examined as well.Results showed that,A83-01 dramatically increased the number of mGSCs and AP positive colonies,and the mitosis index according to the BrdU assay.A83-01 could also increase the expression of pluripotent markers including Oct4,Klf4,Nanog and c-Myc,analyzed byreal-time quantative PCR.mGSCs cultured in the optimal feeder-and serum-free system combined with A83-01 could form embryoid bodies（EBs）,which consisted of three embryonic layers detected by immunofluorescence and RT-PCR.Remarkably,the results demonstrated 0.25 μmol L-1A83-01 could promote the proliferation of mouse mGSC colonies and maintain their undifferentiated status under feeder-and serum-free systems.展开更多
目的探讨银杏酚酸(Ginkgolic Phenols And Acids,GPAA)对体外培养SACC-83的抑制作用,通过MTT法检测其对SACC-83的生长抑制率。方法采用流式细胞仪检测细胞周期变化和细胞凋亡比例,倒置显微镜观察细胞凋亡。结果 GPAA对SACC-83的增殖有...目的探讨银杏酚酸(Ginkgolic Phenols And Acids,GPAA)对体外培养SACC-83的抑制作用,通过MTT法检测其对SACC-83的生长抑制率。方法采用流式细胞仪检测细胞周期变化和细胞凋亡比例,倒置显微镜观察细胞凋亡。结果 GPAA对SACC-83的增殖有抑制作用,且与浓度呈正相关。结论GPAA对SACC-83的生长具有抑制作用,并可诱导细胞凋亡。展开更多
Objective:To evaluate the expression levels and correlations among the expressions of transforming growth factor-β_1(TGF-β_1),Kunx3 and CD83 in colonic mucosal specimens from IBS patients.Methods:A total of 40 patie...Objective:To evaluate the expression levels and correlations among the expressions of transforming growth factor-β_1(TGF-β_1),Kunx3 and CD83 in colonic mucosal specimens from IBS patients.Methods:A total of 40 patients were selected,who were confirmed as IBS by Rome III standard and 40 healthy volunteers served as control.Colonic mucosal specimens of each subject were collected from colon sigmoideum with biopsy forceps.Runx3,TGF-β_1?and CD83(the marker for immunecompetent mature dendritic cells(DCs)mRNA in the sigmoid colon tissue were measured by real-time fluorescence quantitative PCR.Results:Compared with the control group,CD83 mRNA expressions were higher in patients with IBS than in healthy controls(P<0.05)and were associated with runt-related transcription factor 3(Runx3)mRNA levels(r=-0.361,P<0.05).Meanwhile,Runx3 mRNA levels were associated with TGF-β_1,mRNA expressions in irritable bowel syndrome(IBS)patients(r=0.402,P<0.05).However,there was no correlation between the mRNA expressions of TGF-β_1 and CD83(P>0.05).Conclusions:The increase of abnormal dendritic cells might influence the occurrence and development of IBS.TGF-β_1 signal pathway might not be involved in Runx 3-regulated maturation of dendritic cells in IBS.展开更多
基金supported by grants from the National Natural Science Foundation of China, China (30972097, 31272518)the Program for New Century Excellent Talents in University, China (NCET-09-0654)the Fundamental Research Funds for the Central Universities, China (QN2011012)
文摘A83-01 is a selective inhibitor of the TGF-β type I receptor ALK,which inhibits the TGF-β-induced epithelial-to-mesenchymal transition（EMT） via the inhibition of Smad2 phosphorylation.Previous studies have showed that A83-01 promoted somatic cellular reprogramming significantly.Male germline stem cells（mGSCs）,as an alternative resource of pluripotent stem cells derived adult testis,have promising valuable in clinic medicine and regeneration,however,the derivation of mGSCs was complex and difficult.What the role A83-01 plays in promoting the proliferation of mGSCs is still unknown.In this study,combined with A83-01 and knockout serum replacement（KSR） medium,we obtained a relatively feeder-and serum-free system for mGSCs culturing in vitro and the optimal concentration of A83-01 was 0.25 μmol L-1.After continuous culturing,the proliferation efficiency of undifferentiated mGSCs and differentiation capacity of mGSC were examined as well.Results showed that,A83-01 dramatically increased the number of mGSCs and AP positive colonies,and the mitosis index according to the BrdU assay.A83-01 could also increase the expression of pluripotent markers including Oct4,Klf4,Nanog and c-Myc,analyzed byreal-time quantative PCR.mGSCs cultured in the optimal feeder-and serum-free system combined with A83-01 could form embryoid bodies（EBs）,which consisted of three embryonic layers detected by immunofluorescence and RT-PCR.Remarkably,the results demonstrated 0.25 μmol L-1A83-01 could promote the proliferation of mouse mGSC colonies and maintain their undifferentiated status under feeder-and serum-free systems.
文摘目的探讨银杏酚酸(Ginkgolic Phenols And Acids,GPAA)对体外培养SACC-83的抑制作用,通过MTT法检测其对SACC-83的生长抑制率。方法采用流式细胞仪检测细胞周期变化和细胞凋亡比例,倒置显微镜观察细胞凋亡。结果 GPAA对SACC-83的增殖有抑制作用,且与浓度呈正相关。结论GPAA对SACC-83的生长具有抑制作用,并可诱导细胞凋亡。
基金supported by Key Science and Technology Project of Hainan Province(Grant No.ZDXM20100042)
文摘Objective:To evaluate the expression levels and correlations among the expressions of transforming growth factor-β_1(TGF-β_1),Kunx3 and CD83 in colonic mucosal specimens from IBS patients.Methods:A total of 40 patients were selected,who were confirmed as IBS by Rome III standard and 40 healthy volunteers served as control.Colonic mucosal specimens of each subject were collected from colon sigmoideum with biopsy forceps.Runx3,TGF-β_1?and CD83(the marker for immunecompetent mature dendritic cells(DCs)mRNA in the sigmoid colon tissue were measured by real-time fluorescence quantitative PCR.Results:Compared with the control group,CD83 mRNA expressions were higher in patients with IBS than in healthy controls(P<0.05)and were associated with runt-related transcription factor 3(Runx3)mRNA levels(r=-0.361,P<0.05).Meanwhile,Runx3 mRNA levels were associated with TGF-β_1,mRNA expressions in irritable bowel syndrome(IBS)patients(r=0.402,P<0.05).However,there was no correlation between the mRNA expressions of TGF-β_1 and CD83(P>0.05).Conclusions:The increase of abnormal dendritic cells might influence the occurrence and development of IBS.TGF-β_1 signal pathway might not be involved in Runx 3-regulated maturation of dendritic cells in IBS.
