Cell migration is a finely tuned biological process that often involves epithelial-mesenchymal transition(EMT).EMT is typically characterized by the upregulation of mesenchymal markers such as Snail1.This process has ...Cell migration is a finely tuned biological process that often involves epithelial-mesenchymal transition(EMT).EMT is typically characterized by the upregulation of mesenchymal markers such as Snail1.This process has been shown to be of critical importance to normal developmental processes,including neural crest migration and invasion.Interestingly,similar mechanisms are utilized in disease processes,such as tumor metastasis and migration.Notably,EMT and EMT-like processes confer tumor cells with the ability to migrate,invade,and adopt stem cell-like properties that largely account for immunosuppression and tumor recurrence.Therefore,identifying sensitive EMT markers may contribute to cancer prognosis and diagnosis in many facets.Previously,we showed that Elp3 plays an essential role during neural crest migration by stabilizing Snail1.In the current study,we further elucidate the molecular mechanism underlying colorectal cancer migration.We found that mElp3 exerted an identical function to xElp3 in modulating neural crest migration,and both HAT and SAM domains are imperative during this migratory process.Interestingly,overexpression of mElp3 in SW480 cells promoted cell migration and invasion,and we further showed that Elp3 stabilized Snail1 requiring integrity of both SAM and HAT domains.Our findings warrant further exploration of the specific target of Elp3 in cancer cells.展开更多
基于前期研究工作中构建的千里光全长c DNA文库,我们分离到千里光S-腺苷甲硫氨酸合成酶(Sadenosylmethionine synthase,SAMS)基因,本研究分析了该基因开放阅读框,并对该多肽3个高度保守序列形成的结构域和功能位点之间的关系进行了探讨...基于前期研究工作中构建的千里光全长c DNA文库,我们分离到千里光S-腺苷甲硫氨酸合成酶(Sadenosylmethionine synthase,SAMS)基因,本研究分析了该基因开放阅读框,并对该多肽3个高度保守序列形成的结构域和功能位点之间的关系进行了探讨。结果表明,该基因(Gen Bank ID:KC149908.1)编码的蛋白质由394个氨基酸残基组成,理论等电点5.48,相对分子质量43.40 k D;结构域比对和3-D模型分析结果表明,α-helix/β-strand是该蛋白结构域的主要组分,且SPOUT结构与甲基转移酶反应密切相关,其功能可能涉及核酸、蛋白质和磷脂的合成,本研究揭示SAMS的氨基酸保守基序是形成高级结构并决定其生物学功能之关键所在。展开更多
OBJECTIVE:To investigate the relationship between antiviral restriction factor Sterile Alpha Motif and Histidine-Aspartic acid domain-containing protein 1(SAMHD1)expression and T cell activation,furthermore,identifyin...OBJECTIVE:To investigate the relationship between antiviral restriction factor Sterile Alpha Motif and Histidine-Aspartic acid domain-containing protein 1(SAMHD1)expression and T cell activation,furthermore,identifying objective indexes of lung-spleen Qi deficiency symptom pattern.METHODS:We assessed the profile of T lymphocyte subsets,characteristics of SAMHD1 and human leukocyte antigen DR(HLA-DR)expression in lungspleen Qi deficiency patients.At the same time,people living with human immunodeficiency virus/acquired immune deficiency syndrome(HIV/AIDS)(PLWHA)without obvious clinical symptoms and healthy donors in this area were used as controls.RESULTS:Immunohematologic indexes lower CD4 count,lower CD4/CD8 ratio and higher SAMHD1 level were found in lung-spleen Qi deficiency patients.Furthermore,we demonstrated a positive relationship between SAMHD1 and HLA-DR level as well as with interferon factor in lung-spleen Qi deficiency syndrome and patients without obvious clinical signs and symptoms groups.CONCLUSIONS:These data indicated the positive relationship between SAMHD1 and T cell activation which further elucidated the role of SAMHD1 in cellular immune response.Furthermore,combination of T lymphocyte subsets counts and SAMHD1 level may be used as clinical and biological reference basis for the differentiation and diagnosis of HIV/AIDS traditional Chinese medicine syndromes.展开更多
LATERAL ORGAN BOUNDARIES DOMAIN(LBD)基因家族是在拟南芥中发现的高等植物所特有的一类基因,编码的蛋白中含有LATERAL ORGAN BOUNDARIES(LOB)结构域。LBD基因一般在侧生器官与茎尖分生组织的边界处、侧生器官的近轴面一侧的基部表达,...LATERAL ORGAN BOUNDARIES DOMAIN(LBD)基因家族是在拟南芥中发现的高等植物所特有的一类基因,编码的蛋白中含有LATERAL ORGAN BOUNDARIES(LOB)结构域。LBD基因一般在侧生器官与茎尖分生组织的边界处、侧生器官的近轴面一侧的基部表达,并呈现出在多种组织内特异性表达的特征,暗示该类基因可能在植物的多种发育过程中发挥功能。LBD蛋白结构中除含有上述LOB结构域以外,尚未发现其它已知功能的结构域的存在。目前,已经在拟南芥中发现43个LBD基因,而在玉米和水稻中各有35和43个LBD基因。根据LBD蛋白结构中是否含有亮氨酸拉链类似基序,将LBD基因分为两类:第一类(class I)LBD蛋白结构域中包含完整亮氨酸拉链基序;第二类(class II)LBD蛋白结构域中不含亮氨酸拉链基序。本文就LBD基因的结构以及它们对高等植物生长发育的影响、LBD基因和植物激素的关系、LBD基因与miRNA的关系进行了系统的总结。展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.81760503 and 81760660)the Applied Basic Research Key Project of Yunnan(Grant Nos.2018FE001(-318)and 2018FE001(-123))the Yunnan Health Science and Technology Plan Projects(Grant No.2016NS207).
文摘Cell migration is a finely tuned biological process that often involves epithelial-mesenchymal transition(EMT).EMT is typically characterized by the upregulation of mesenchymal markers such as Snail1.This process has been shown to be of critical importance to normal developmental processes,including neural crest migration and invasion.Interestingly,similar mechanisms are utilized in disease processes,such as tumor metastasis and migration.Notably,EMT and EMT-like processes confer tumor cells with the ability to migrate,invade,and adopt stem cell-like properties that largely account for immunosuppression and tumor recurrence.Therefore,identifying sensitive EMT markers may contribute to cancer prognosis and diagnosis in many facets.Previously,we showed that Elp3 plays an essential role during neural crest migration by stabilizing Snail1.In the current study,we further elucidate the molecular mechanism underlying colorectal cancer migration.We found that mElp3 exerted an identical function to xElp3 in modulating neural crest migration,and both HAT and SAM domains are imperative during this migratory process.Interestingly,overexpression of mElp3 in SW480 cells promoted cell migration and invasion,and we further showed that Elp3 stabilized Snail1 requiring integrity of both SAM and HAT domains.Our findings warrant further exploration of the specific target of Elp3 in cancer cells.
文摘基于前期研究工作中构建的千里光全长c DNA文库,我们分离到千里光S-腺苷甲硫氨酸合成酶(Sadenosylmethionine synthase,SAMS)基因,本研究分析了该基因开放阅读框,并对该多肽3个高度保守序列形成的结构域和功能位点之间的关系进行了探讨。结果表明,该基因(Gen Bank ID:KC149908.1)编码的蛋白质由394个氨基酸残基组成,理论等电点5.48,相对分子质量43.40 k D;结构域比对和3-D模型分析结果表明,α-helix/β-strand是该蛋白结构域的主要组分,且SPOUT结构与甲基转移酶反应密切相关,其功能可能涉及核酸、蛋白质和磷脂的合成,本研究揭示SAMS的氨基酸保守基序是形成高级结构并决定其生物学功能之关键所在。
基金Supported by the National Natural Science Foundation of China(Construction of HIV Pseudovirus Infected Cell Model and Application of Yiaikang in the Study of T Cell Immune Response,No.82004207)the National Special Science and Technology Program on Major Infectious Diseases(Study on the Scheme of TCM to Reduce the Incidence of Adverse Reactions to AIDS Antiviral Therapy,No.2017ZX10205502002+6 种基金Study on the Scheme of TCM to Reduce the Incidence of AIDS Opportunistic Infection,No.2017ZX10205502003)the Science and Technology Tackling Plan of Henan Province(Exploring the Activation Mechanism of Yiaikang on CD8+T Lymphocytes in HIV/AIDS Patients based on STING/SAMHD1 Pathway,No.202102310178Regulation of HIV/AIDS Treg CellDerived Exosomeso on Activation of CD4+T Cells and the Intervention of Yiaikang,No.212102311126)Subject Construction Project of Henan Characteristic Backbone Subject of TCM of Henan University of TCM(Deep Sequencing/Molecular Docking/Gene Editing Network System to Analyze The Mechanism of TCM Regulating the Immune Function of HIV Patients,No.STG-ZYXKY-2020029)Henan Province TCM Research Project(Explore the Mechanism of Yiaikang in the Treatment of AIDS Immunity Based on HIV gag-CTL Response,No.2018JDZX106,2019AZB003Study on TCM Treatment of Aged AIDS,No.20-21ZYZD03)Key Scientific Research Projects of Colleges and Universities in Henan Province(to Explore the Regulation Mechanism of Yiaikang on AIDS Lung Qi Deficiency Syndrome from TGF-β/Smad/ID,No.20A360010)
文摘OBJECTIVE:To investigate the relationship between antiviral restriction factor Sterile Alpha Motif and Histidine-Aspartic acid domain-containing protein 1(SAMHD1)expression and T cell activation,furthermore,identifying objective indexes of lung-spleen Qi deficiency symptom pattern.METHODS:We assessed the profile of T lymphocyte subsets,characteristics of SAMHD1 and human leukocyte antigen DR(HLA-DR)expression in lungspleen Qi deficiency patients.At the same time,people living with human immunodeficiency virus/acquired immune deficiency syndrome(HIV/AIDS)(PLWHA)without obvious clinical symptoms and healthy donors in this area were used as controls.RESULTS:Immunohematologic indexes lower CD4 count,lower CD4/CD8 ratio and higher SAMHD1 level were found in lung-spleen Qi deficiency patients.Furthermore,we demonstrated a positive relationship between SAMHD1 and HLA-DR level as well as with interferon factor in lung-spleen Qi deficiency syndrome and patients without obvious clinical signs and symptoms groups.CONCLUSIONS:These data indicated the positive relationship between SAMHD1 and T cell activation which further elucidated the role of SAMHD1 in cellular immune response.Furthermore,combination of T lymphocyte subsets counts and SAMHD1 level may be used as clinical and biological reference basis for the differentiation and diagnosis of HIV/AIDS traditional Chinese medicine syndromes.
文摘LATERAL ORGAN BOUNDARIES DOMAIN(LBD)基因家族是在拟南芥中发现的高等植物所特有的一类基因,编码的蛋白中含有LATERAL ORGAN BOUNDARIES(LOB)结构域。LBD基因一般在侧生器官与茎尖分生组织的边界处、侧生器官的近轴面一侧的基部表达,并呈现出在多种组织内特异性表达的特征,暗示该类基因可能在植物的多种发育过程中发挥功能。LBD蛋白结构中除含有上述LOB结构域以外,尚未发现其它已知功能的结构域的存在。目前,已经在拟南芥中发现43个LBD基因,而在玉米和水稻中各有35和43个LBD基因。根据LBD蛋白结构中是否含有亮氨酸拉链类似基序,将LBD基因分为两类:第一类(class I)LBD蛋白结构域中包含完整亮氨酸拉链基序;第二类(class II)LBD蛋白结构域中不含亮氨酸拉链基序。本文就LBD基因的结构以及它们对高等植物生长发育的影响、LBD基因和植物激素的关系、LBD基因与miRNA的关系进行了系统的总结。
