To determine whether squamous cell carcin-oma antigen recognized by human T cell 3(SART3)gene can induce antitumor immunity against tumor cells which express the gene,we constructed mouse tumor cells expressing human ...To determine whether squamous cell carcin-oma antigen recognized by human T cell 3(SART3)gene can induce antitumor immunity against tumor cells which express the gene,we constructed mouse tumor cells expressing human SART3(LM8-SART3)and carried out experiments in vitro/vivo.After subcutaneous injection with SART3 gene vaccine,cytotoxic T lymphocyte(CTL)activity in vitro was measured using Cell Counting Kit-8.As for the in vivo part,C3H mice were divided into several groups.One group was challenged with tumor cells after immunity.Another group was treated with the vaccine after tumor implantation.It was found that human SART3 DNA vaccine can elicit a specific CTL reaction from the mouse splenocytes.After vaccination,tumor occurrence and tumor growth speed was reduced.The vaccine also shows activity in tumor treatment.We con-clude that the human SART3 DNA vaccine can induce antitumor ability against tumor cells expressing human SART3(LM8-SART3)in vitro/vivo which may provide new possibilities in antitumor therapy.展开更多
本文提出了一种基于导引图像滤波(Guided Image Filtering,GIF)和截断全变分(Truncated Total Variation,截断TV)的CT重建算法,称为SART-TTV-GIF算法。导引图像滤波是一个具有边缘保护作用的平滑算子,可在保持边缘特征的同时去除噪声,...本文提出了一种基于导引图像滤波(Guided Image Filtering,GIF)和截断全变分(Truncated Total Variation,截断TV)的CT重建算法,称为SART-TTV-GIF算法。导引图像滤波是一个具有边缘保护作用的平滑算子,可在保持边缘特征的同时去除噪声,它通过把基于截断全变分的算法所重建的图像作为导引图像,然后利用导引图像滤波把导引图像含有的重要特征转移到SART算法重建的图像中,使滤波后的图像和导引图像的重要特征具有相似性,从而提高重建图像的质量。本文用高分辨率的头部模型进行了投影数据无噪声和有噪声实验,实验结果表明,本文提出的SART-TTV-GIF算法与SART算法、SART-TV算法、SART-TTV算法和SART-TV-GIF算法相比,重建的图像质量较高。展开更多
基金This study was supported by the Natural Science Foundation Project of the Health Department of Hubei Province,China.(No.NX200509)。
文摘To determine whether squamous cell carcin-oma antigen recognized by human T cell 3(SART3)gene can induce antitumor immunity against tumor cells which express the gene,we constructed mouse tumor cells expressing human SART3(LM8-SART3)and carried out experiments in vitro/vivo.After subcutaneous injection with SART3 gene vaccine,cytotoxic T lymphocyte(CTL)activity in vitro was measured using Cell Counting Kit-8.As for the in vivo part,C3H mice were divided into several groups.One group was challenged with tumor cells after immunity.Another group was treated with the vaccine after tumor implantation.It was found that human SART3 DNA vaccine can elicit a specific CTL reaction from the mouse splenocytes.After vaccination,tumor occurrence and tumor growth speed was reduced.The vaccine also shows activity in tumor treatment.We con-clude that the human SART3 DNA vaccine can induce antitumor ability against tumor cells expressing human SART3(LM8-SART3)in vitro/vivo which may provide new possibilities in antitumor therapy.
文摘本文提出了一种基于导引图像滤波(Guided Image Filtering,GIF)和截断全变分(Truncated Total Variation,截断TV)的CT重建算法,称为SART-TTV-GIF算法。导引图像滤波是一个具有边缘保护作用的平滑算子,可在保持边缘特征的同时去除噪声,它通过把基于截断全变分的算法所重建的图像作为导引图像,然后利用导引图像滤波把导引图像含有的重要特征转移到SART算法重建的图像中,使滤波后的图像和导引图像的重要特征具有相似性,从而提高重建图像的质量。本文用高分辨率的头部模型进行了投影数据无噪声和有噪声实验,实验结果表明,本文提出的SART-TTV-GIF算法与SART算法、SART-TV算法、SART-TTV算法和SART-TV-GIF算法相比,重建的图像质量较高。
