The quality of fish sperm is an important factor affecting artificial reproduction.Since the sexual maturation of male and female is not synchronized or for the fish that need to kill the male fish for sperm,sperm pre...The quality of fish sperm is an important factor affecting artificial reproduction.Since the sexual maturation of male and female is not synchronized or for the fish that need to kill the male fish for sperm,sperm preservation solution is used to preserve the sperm temporarily during reproduction,in order to meet the needs of large-scale reproduction.Therefore,the preservation effect of sperm preservation solution directly affects the artificial reproduction.The quality of sperm in the preservation solution can be judged macroscopically by microscopic examination,but it is not accurate,while sperm chromatin structure analysis(SCSA)can quantitatively judge the quality of sperm in the preservation solution,thus providing guarantee for reproduction.The Zhaos sperm preservation solution of Pseudobagrus hwanghoensis has been verified by artificial breeding and SCSA analysis for many times,and it is better than Hanks sperm preservation solution.When the DNA fragmentation index is less than or equal to 0.15,the sperm is vigorous and the effect is obvious.展开更多
目的探讨连续顺序追踪超声法（SCSA）在诊断胎儿肢体畸形中的价值。方法采用GE Vo Lu So NE8对2013年1月至2014年12月住院的18331例孕妇行SCSA对胎儿肢体进行检查,对超声检查情况及不同孕周SCSA法检出胎儿肢体畸形的情况进行分析。结果 ...目的探讨连续顺序追踪超声法（SCSA）在诊断胎儿肢体畸形中的价值。方法采用GE Vo Lu So NE8对2013年1月至2014年12月住院的18331例孕妇行SCSA对胎儿肢体进行检查,对超声检查情况及不同孕周SCSA法检出胎儿肢体畸形的情况进行分析。结果 （1）经SCSA法检查之后,本研究中的18331例胎儿中,发现59例胎儿肢体畸形,后经临床随访,有64例胎儿肢体畸形。SCSA法诊断准确率为92.19%（59/64）;（2）孕周在19～27周SCSA法检出胎儿畸形最多（84.76%）,而孕周〈19周、19～〈27周、27～〈31周、〉31周SCSA检出胎儿肢体畸形率均较少。结论 SCSA法在诊断胎儿肢体畸形具有较高的应用价值,应在临床上进行推广。展开更多
Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic...Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife＇s antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay （SCSA） and the terminal deoxynucleotidyl transferase dUTP nick end labelling （TUNEL） assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count （P〈O.01）, more motile sperms （P=-O.04） and fewer sperm head defects （P=-O.05）. These associations were consistent within and across study regions. Furthermore, testosterone, follicle-stimulating hormone （FSH） and sexual hormone-binding globulin （SHBG） were significantly negatively correlated with Fas within and across regions as well. The data indicated no association between the anti-apoptotic Bcl-xL marker and semen or personal characteristics. The finding of Fas-positive sperm cells associated with better semen quality in a cohort of spouses of pregnant women seems different from previous data obtained in infertile men and warrants further investigation to clarify the biological significance of sperm apoptotic markers.展开更多
基金Key Scientific and Technological Research Project of Henan Province(172102110097).
文摘The quality of fish sperm is an important factor affecting artificial reproduction.Since the sexual maturation of male and female is not synchronized or for the fish that need to kill the male fish for sperm,sperm preservation solution is used to preserve the sperm temporarily during reproduction,in order to meet the needs of large-scale reproduction.Therefore,the preservation effect of sperm preservation solution directly affects the artificial reproduction.The quality of sperm in the preservation solution can be judged macroscopically by microscopic examination,but it is not accurate,while sperm chromatin structure analysis(SCSA)can quantitatively judge the quality of sperm in the preservation solution,thus providing guarantee for reproduction.The Zhaos sperm preservation solution of Pseudobagrus hwanghoensis has been verified by artificial breeding and SCSA analysis for many times,and it is better than Hanks sperm preservation solution.When the DNA fragmentation index is less than or equal to 0.15,the sperm is vigorous and the effect is obvious.
文摘目的探讨连续顺序追踪超声法（SCSA）在诊断胎儿肢体畸形中的价值。方法采用GE Vo Lu So NE8对2013年1月至2014年12月住院的18331例孕妇行SCSA对胎儿肢体进行检查,对超声检查情况及不同孕周SCSA法检出胎儿肢体畸形的情况进行分析。结果 （1）经SCSA法检查之后,本研究中的18331例胎儿中,发现59例胎儿肢体畸形,后经临床随访,有64例胎儿肢体畸形。SCSA法诊断准确率为92.19%（59/64）;（2）孕周在19～27周SCSA法检出胎儿畸形最多（84.76%）,而孕周〈19周、19～〈27周、27～〈31周、〉31周SCSA检出胎儿肢体畸形率均较少。结论 SCSA法在诊断胎儿肢体畸形具有较高的应用价值,应在临床上进行推广。
文摘Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife＇s antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay （SCSA） and the terminal deoxynucleotidyl transferase dUTP nick end labelling （TUNEL） assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count （P〈O.01）, more motile sperms （P=-O.04） and fewer sperm head defects （P=-O.05）. These associations were consistent within and across study regions. Furthermore, testosterone, follicle-stimulating hormone （FSH） and sexual hormone-binding globulin （SHBG） were significantly negatively correlated with Fas within and across regions as well. The data indicated no association between the anti-apoptotic Bcl-xL marker and semen or personal characteristics. The finding of Fas-positive sperm cells associated with better semen quality in a cohort of spouses of pregnant women seems different from previous data obtained in infertile men and warrants further investigation to clarify the biological significance of sperm apoptotic markers.
