Hydrogels composed of natural polysaccharides hold great potential as a release system for delivery of small molecule drugs. In this study, the functional drug sphingosine 1-phosphate （SIP） was loaded in the hydroge...Hydrogels composed of natural polysaccharides hold great potential as a release system for delivery of small molecule drugs. In this study, the functional drug sphingosine 1-phosphate （SIP） was loaded in the hydrogel which was derived from aldehyde hyaluroulc acid （AHA） and carboxymethyl chitosun （CC） through Schiff＇s base reaction for promoting ungiogenesis. The scanning electron microscopy （SEM） images demonstrated the suitable threedimension network structure of hydrogel. The rheological properties and compressive stress-strain behavior of hydrngel were also examined, and the results indicated that the hydrogei possessed good mechanical properties. In vitro drug release behaviors showed that drug released from the hydrogei in a sustained manner and could achieve prolonged release time compared to the pure drug. The 3- （ 4, 5-dimethylthiazol-2-yl ） -2, 5-diphenyl （MTT） assay showed the good cytocompatibility of the resulting hydrogel. More importantly, the chicken chorioallantoic membrane （CAM） assay confirmed that the SIP loaded hydrogel could significantly promote angingenesis in the CAM model. As a result, our results strongly suggested that the as-prepared hydrogel would be a good candidate for delivery of functional drugs.展开更多
基金National Natural Science Foundations of China(Nos.31271028,31570984)International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality,China(No.15540723400)+2 种基金Open Foundation of State Key Laboratory for Modification of Chemical Fibers,Polymer Materials,China(No.LK1416)the Innovation Funds of Donghua University,China(No.15D310516)“111 Project”Biomedical Textile Materials Science and Technology,China(No.B07024)
文摘Hydrogels composed of natural polysaccharides hold great potential as a release system for delivery of small molecule drugs. In this study, the functional drug sphingosine 1-phosphate （SIP） was loaded in the hydrogel which was derived from aldehyde hyaluroulc acid （AHA） and carboxymethyl chitosun （CC） through Schiff＇s base reaction for promoting ungiogenesis. The scanning electron microscopy （SEM） images demonstrated the suitable threedimension network structure of hydrogel. The rheological properties and compressive stress-strain behavior of hydrngel were also examined, and the results indicated that the hydrogei possessed good mechanical properties. In vitro drug release behaviors showed that drug released from the hydrogei in a sustained manner and could achieve prolonged release time compared to the pure drug. The 3- （ 4, 5-dimethylthiazol-2-yl ） -2, 5-diphenyl （MTT） assay showed the good cytocompatibility of the resulting hydrogel. More importantly, the chicken chorioallantoic membrane （CAM） assay confirmed that the SIP loaded hydrogel could significantly promote angingenesis in the CAM model. As a result, our results strongly suggested that the as-prepared hydrogel would be a good candidate for delivery of functional drugs.
