The Expression of Endothelin Receptor B in Melanoma Cells A375 and Sk-mel-1 and the Proliferative Effects of Endothelin 3 on A375 Cells

In order to investigate the expression of endothelin receptor B （ETR-B） in human malignant melanoma （MM） cells A375 and SK-mel-1 and the proliferative effects of endothelin 3 （ET3） on A375 cells, RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1. MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro. The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells. ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a concentration-dependent manner. It was suggested that ET3/ETR-B might play an important proliferative role in MM.

内皮素受体B在黑素瘤中的表达及内皮素3对黑素瘤细胞A375的体外促生长效应

目的研究内皮素受体B(ETR-B)在人恶性黑素瘤中的表达及内皮素3对黑素瘤细胞A375的体外促生长效应。方法采用免疫组化SP法检测ETR-B在黑素瘤组织中的表达,用RT-PCR法检测ETR-B基因在人恶性黑素瘤细胞A375和SK-mel-1中的表达,用MTT比色法检测不同浓度内皮素3对黑素瘤细胞A375的体外促增殖活性。结果ETR-B在41例恶性黑素瘤和23例色素痣中的阳性率分别为78.05%和8.69%,两组间差异有统计学意义。P<0.05。15例原位和26例Ⅰ～Ⅳ期恶性黑素瘤ETR-B阳性表达率分别为53.33%和92.31%,两组比较.P<0.05。13例转移性恶性黑素瘤(Ⅲ～Ⅳ期)中ETR-B阳性表达率100%,28例未转移者(0～Ⅱ期)的阳性表达率为67.86%,两组比较,P<0.05。ETR-B基因在人恶性黑素瘤细胞A375和SK-mel-1中均有表达;内皮素3对黑素瘤细胞A375在体外具有很强的促增殖作用,且促增殖能力呈内皮素3浓度依赖性。结论内皮素3/ETR-B在促黑素瘤细胞生长中有重要作用。