目的研究乳癌临床常用化疗药物顺铂(cisplatin or DDP),阿霉素(adriamycin,ADM),氟尿嘧啶(fluorouracil,5Fu)对MDA-MB231乳癌细胞SNCG表达的干扰效应。方法通过RT-PCR及免疫组织化学法检测上述药物处理组和阴性对照组MDA-MB231细胞的SNC...目的研究乳癌临床常用化疗药物顺铂(cisplatin or DDP),阿霉素(adriamycin,ADM),氟尿嘧啶(fluorouracil,5Fu)对MDA-MB231乳癌细胞SNCG表达的干扰效应。方法通过RT-PCR及免疫组织化学法检测上述药物处理组和阴性对照组MDA-MB231细胞的SNCG表达状况,用Quantity One软件及北航真彩色医学图像处理系统(CM-2000B)分别对各组SNCG mRNA和蛋白相对表达水平进行分析。结果ADM和5Fu处理组与阴性对照组比较,MDA-MB231细胞的SNCG mRNA及蛋白表达水平差异均有统计学意义(P值均小于0.05),而DDP处理组表达水平与对照组无差别。结论ADM和5Fu可抑制MDA-MB231细胞的SNCG表达。展开更多
Objective:To investigate the correlation between periostin and SNCG and esophageal cancer invasion,infiltration and apoptosis.Methods:A total of 78 cases esophageal surgical resection specimens were collected,expressi...Objective:To investigate the correlation between periostin and SNCG and esophageal cancer invasion,infiltration and apoptosis.Methods:A total of 78 cases esophageal surgical resection specimens were collected,expression of periostin and SNCG in esophageal cancer were detected. Effect of periostin and SNCG in esophageal carcinoma invasion and infiltration was analyzed. Results:The upregulated rate of periostin had significant difference in esophageal cancer tissues (39.74%),adjacent tissues(17.86%) and normal tissues(0.00%);The positive expression rates of SNCG had significant difference in esophageal cancer tissues(61.54%),adjacent tissues(32.14% and normal tissues 11.96%);The upreguialed rate of perioslin had a significant correlation with lymph node metastasis,adventitia invasion.TNM stage:The positive expression rates of SNCG had a significant correlation with differentiation degree,lymph node metastasis,adventitia invasion.TNM stage:Apoptosis index ol the positive of expression ol SNCG of esophageal cancer tissue(4.541±2.267) was significantly lower than that of the negative expression(7.316±2.582) (P【0.05).Conclusions:SNCG may play an important role in invasion,infiltration and apoptosis of esophageal cancer and serve as larget spots in the targeted therapy of esophageal cancer.展开更多
Objective:The aim of the study was to evaluate the inhibition of different chemotherapy drugs on γ-synuclein (SNCG) positive expression of breast cancer cell line MDA-MB231,and the effects on cell cycle and apoptosis...Objective:The aim of the study was to evaluate the inhibition of different chemotherapy drugs on γ-synuclein (SNCG) positive expression of breast cancer cell line MDA-MB231,and the effects on cell cycle and apoptosis,and to explore the related mechanism as well.Methods:We treated the breast cancer cell line MDA-MB231 for the inhibition of SNCG with chemotherapy drugs such as irinotecan,nedaplatin and 5-fluorouracil using RT-PCR and immunohistochemistry,and adopted flow cytometry to detect cell cycle distribution and apoptosis.Results:At the transcription and translation levels,the SNCG expression level in nedaplatin group and 5-fluorouracil group was lower than that of other groups and there was statistically significance (P < 0.01) compared with the control group,while there was not statistically significant between irinotecan group and the control group.After drugs action,cell cycle and distribution in each experiment group changed obviously,where the cells in G0G1 phase increased,especially the cells in the nedaplatin group and 5-fluorouracil group changed most significantly,as well as the obvious change in the cells of nedaplatin group and 5-fluorouracil group in the apoptosis period.Conclusion:There was a stronger inhibition of SNCG expression in nedaplatin and 5-fluorouracil groups,and can cause significant cell cycle and apoptosis changes.It may also be concluded that nedaplatin and 5-fluorouracil could make effects by the mechanisms of inhibiting cancer cell proliferation and inducing cell apoptosis.展开更多
基金supported by China International Medical Forndation special Foundation(Grant No:CIMF-F-H001-225)Science and technology commision of Shanghai medical guide Foundations(Grant No:114119a7700)
文摘Objective:To investigate the correlation between periostin and SNCG and esophageal cancer invasion,infiltration and apoptosis.Methods:A total of 78 cases esophageal surgical resection specimens were collected,expression of periostin and SNCG in esophageal cancer were detected. Effect of periostin and SNCG in esophageal carcinoma invasion and infiltration was analyzed. Results:The upregulated rate of periostin had significant difference in esophageal cancer tissues (39.74%),adjacent tissues(17.86%) and normal tissues(0.00%);The positive expression rates of SNCG had significant difference in esophageal cancer tissues(61.54%),adjacent tissues(32.14% and normal tissues 11.96%);The upreguialed rate of perioslin had a significant correlation with lymph node metastasis,adventitia invasion.TNM stage:The positive expression rates of SNCG had a significant correlation with differentiation degree,lymph node metastasis,adventitia invasion.TNM stage:Apoptosis index ol the positive of expression ol SNCG of esophageal cancer tissue(4.541±2.267) was significantly lower than that of the negative expression(7.316±2.582) (P【0.05).Conclusions:SNCG may play an important role in invasion,infiltration and apoptosis of esophageal cancer and serve as larget spots in the targeted therapy of esophageal cancer.
基金Supported by the grant of Youth Fund Projects of Sichuan Provincial Department of Education (No. 11ZB163)
文摘Objective:The aim of the study was to evaluate the inhibition of different chemotherapy drugs on γ-synuclein (SNCG) positive expression of breast cancer cell line MDA-MB231,and the effects on cell cycle and apoptosis,and to explore the related mechanism as well.Methods:We treated the breast cancer cell line MDA-MB231 for the inhibition of SNCG with chemotherapy drugs such as irinotecan,nedaplatin and 5-fluorouracil using RT-PCR and immunohistochemistry,and adopted flow cytometry to detect cell cycle distribution and apoptosis.Results:At the transcription and translation levels,the SNCG expression level in nedaplatin group and 5-fluorouracil group was lower than that of other groups and there was statistically significance (P < 0.01) compared with the control group,while there was not statistically significant between irinotecan group and the control group.After drugs action,cell cycle and distribution in each experiment group changed obviously,where the cells in G0G1 phase increased,especially the cells in the nedaplatin group and 5-fluorouracil group changed most significantly,as well as the obvious change in the cells of nedaplatin group and 5-fluorouracil group in the apoptosis period.Conclusion:There was a stronger inhibition of SNCG expression in nedaplatin and 5-fluorouracil groups,and can cause significant cell cycle and apoptosis changes.It may also be concluded that nedaplatin and 5-fluorouracil could make effects by the mechanisms of inhibiting cancer cell proliferation and inducing cell apoptosis.
