A polydiacetylene nanocrystalline film has been fabricated using surface evaporationmethod and observed by using Aurora 2000, TopoMetrix scanning near-field optical microscope(SNOM) system. The SNOM images of this fil...A polydiacetylene nanocrystalline film has been fabricated using surface evaporationmethod and observed by using Aurora 2000, TopoMetrix scanning near-field optical microscope(SNOM) system. The SNOM images of this film together with the standard testing Al sampleindicate that the resolution of the system is better than 80 nm 1/6 of the incident wavelength,488 nm. The possibility of SNOM data storage using thus fabricated film is also demonstrated.展开更多
文摘扫描近场光学显微镜突破衍射极限,具有纳米量级的空间分辨率,量子点(QD s)标记有荧光强度高且抗光漂白能力强等优点。结合上述两种技术,对人胃腺癌SGC-7901细胞膜表面特异性结合的叶酸受体(FR)进行成像探测,获得了叶酸受体在SGC-7901细胞膜表面上的分布,以及细胞内化外源性叶酸过程中叶酸受体在细胞膜表面的分布变化,成像的光学分辨率达到120 nm。实验结果表明:特异性结合的叶酸受体在SGC-7901细胞膜表面的分布,绝大部分是以聚集体的形式存在。随着SGC-7901细胞内化叶酸量的增加,叶酸受体在细胞膜表面的分布密度逐渐降低,并在经过120 m in左右趋于稳定。上述方法和手段为实现单细胞水平上靶点分布和变化的长期监测,肿瘤细胞内化受体的机制研究提供了新的技术途径。
文摘A polydiacetylene nanocrystalline film has been fabricated using surface evaporationmethod and observed by using Aurora 2000, TopoMetrix scanning near-field optical microscope(SNOM) system. The SNOM images of this film together with the standard testing Al sampleindicate that the resolution of the system is better than 80 nm 1/6 of the incident wavelength,488 nm. The possibility of SNOM data storage using thus fabricated film is also demonstrated.
