蓝藻Synechococcussp.PCC7942 HCO3-高亲和转运蛋白操纵子基因cmpABCD是其CO2浓缩机制中的调控基因之一.本研究用携带潮霉素B磷酸转移酶基因(hygromyc in B pho transferase,hpt)筛选标记的同源双臂整合载体pUC-HATH转化蓝藻Synechococc...蓝藻Synechococcussp.PCC7942 HCO3-高亲和转运蛋白操纵子基因cmpABCD是其CO2浓缩机制中的调控基因之一.本研究用携带潮霉素B磷酸转移酶基因(hygromyc in B pho transferase,hpt)筛选标记的同源双臂整合载体pUC-HATH转化蓝藻Synechococcussp.PCC7942,以潮霉素B作为筛选试剂筛选出具潮霉素B抗性的转化藻,运用引物PCR方法证实潮霉素B磷酸转移酶基因表达盒通过质粒pUC-HATH的介导已定点插入蓝藻Synechococcussp.PCC7942基因组中,成功地构建了具有潮霉素B抗性的cmpBCD基因插入失活突变藻株.并最终通过比较野生藻Synechococcussp.PCC7942和突变藻Synechococcussp.PCC7942在不同Na2CO3浓度的改良BG-11培养基中生长特性,探讨了HCO3-高亲和转运蛋白操纵子cmpABCD基因失活对藻体生长的影响.展开更多
Biological CO2 sequestration by microalgae is a promising and environmentally friendly technology applied to sequester CO2. The characteristics of neutral lipid accumulation by two marine oil-rich microalgal strains,n...Biological CO2 sequestration by microalgae is a promising and environmentally friendly technology applied to sequester CO2. The characteristics of neutral lipid accumulation by two marine oil-rich microalgal strains,namely, Isochrysis galbana and Nannochloropsis sp., through CO2 enrichment cultivation were investigated in this study. The optimum culture conditions of the two microalgal strains are 10% CO2 and f medium. The maximum biomass productivity, total lipid content, maximum lipid productivity, carbon content, and CO2 fixation ability of the two microalgal strains were obtained. The corresponding parameters of the two strains were as follows：（（142.42±4.58） g/（m^2·d）,（149.92±1.80） g/（m^2·d））,（（39.95±0.77）%,（37.91±0.58）%）,（（84.47±1.56） g/（m^2·d）,（89.90±1.98） g/（m^2·d））,（（45.98±1.75）%,（46.88±2.01）%）, and（（33.74±1.65） g/（m^2·d）,（34.08±1.32） g/（m^2·d））. Results indicated that the two marine microalgal strains with high CO2 fixation ability are potential strains for marine biodiesel development coupled with CO2 emission reduction.展开更多
Enrichment of bacteria capable of growing with electrochemical reducing power and CO2 was accomplished using a plate-type electrochemical bioreactor (PEB). A bacterial source obtained from wastewater treatment reactan...Enrichment of bacteria capable of growing with electrochemical reducing power and CO2 was accomplished using a plate-type electrochemical bioreactor (PEB). A bacterial source obtained from wastewater treatment reactant and forest soil was cultivated on carbonate-based mineral agar medium prepared in the PEB (PEB-carbonate agar). According to the pyrosequencing analyses, the abundance of Betaproteobacteria and Gammaproteobacteria at the phylum level, and Achromobacter, Alcaligenes, and Pseudomonas at the genus level were selectively increased after the electrochemical enrichment culture. Finally, one genus of bacterium that was autotrophically grown on the PEB-carbonate agar was identified as Alcaligenes. This bacterium may be useful to fix atmospheric CO2 with electrochemical energy obtained from the solar cell.展开更多
Reductions in CO2 emissions have a significant effect on the transportation sector, and there is increasing interest in developing green cars such as electric cars. To prepare for the advent of the electric car era, i...Reductions in CO2 emissions have a significant effect on the transportation sector, and there is increasing interest in developing green cars such as electric cars. To prepare for the advent of the electric car era, it will be necessary to predict the increase in electricity demand owing to the spread of electric cars and determine the policy approaches. Therefore, the analysis was performed to promote the use of electric car that helps reduce CO2 emissions. This study establishes a mode choice model using the stated preference method. To improve the predictive power of the model, some revealed preference data were also examined to consider the characteristics of the commuters and the extent of current electric car technology to determine and verify the parameters of the mode choice models. This was used to estimate changes in CO2 emissions owing to the introduction of electric cars and present effective policy approaches to reduce CO2 emissions.展开更多
文摘蓝藻Synechococcussp.PCC7942 HCO3-高亲和转运蛋白操纵子基因cmpABCD是其CO2浓缩机制中的调控基因之一.本研究用携带潮霉素B磷酸转移酶基因(hygromyc in B pho transferase,hpt)筛选标记的同源双臂整合载体pUC-HATH转化蓝藻Synechococcussp.PCC7942,以潮霉素B作为筛选试剂筛选出具潮霉素B抗性的转化藻,运用引物PCR方法证实潮霉素B磷酸转移酶基因表达盒通过质粒pUC-HATH的介导已定点插入蓝藻Synechococcussp.PCC7942基因组中,成功地构建了具有潮霉素B抗性的cmpBCD基因插入失活突变藻株.并最终通过比较野生藻Synechococcussp.PCC7942和突变藻Synechococcussp.PCC7942在不同Na2CO3浓度的改良BG-11培养基中生长特性,探讨了HCO3-高亲和转运蛋白操纵子cmpABCD基因失活对藻体生长的影响.
基金The Basic Scientific Fund for National Public Research Institutes of China under contract Nos 2017Q09 and2016Q02the National Natural Science Foundation of China under contract No.41776176+2 种基金the National Key Research and Development Program of China under contract No.2017YFC1404604the Shandong Provincial Natural Science Foundation under contract No.ZR2015PD003the 2012 Taishan Scholar
文摘Biological CO2 sequestration by microalgae is a promising and environmentally friendly technology applied to sequester CO2. The characteristics of neutral lipid accumulation by two marine oil-rich microalgal strains,namely, Isochrysis galbana and Nannochloropsis sp., through CO2 enrichment cultivation were investigated in this study. The optimum culture conditions of the two microalgal strains are 10% CO2 and f medium. The maximum biomass productivity, total lipid content, maximum lipid productivity, carbon content, and CO2 fixation ability of the two microalgal strains were obtained. The corresponding parameters of the two strains were as follows：（（142.42±4.58） g/（m^2·d）,（149.92±1.80） g/（m^2·d））,（（39.95±0.77）%,（37.91±0.58）%）,（（84.47±1.56） g/（m^2·d）,（89.90±1.98） g/（m^2·d））,（（45.98±1.75）%,（46.88±2.01）%）, and（（33.74±1.65） g/（m^2·d）,（34.08±1.32） g/（m^2·d））. Results indicated that the two marine microalgal strains with high CO2 fixation ability are potential strains for marine biodiesel development coupled with CO2 emission reduction.
文摘Enrichment of bacteria capable of growing with electrochemical reducing power and CO2 was accomplished using a plate-type electrochemical bioreactor (PEB). A bacterial source obtained from wastewater treatment reactant and forest soil was cultivated on carbonate-based mineral agar medium prepared in the PEB (PEB-carbonate agar). According to the pyrosequencing analyses, the abundance of Betaproteobacteria and Gammaproteobacteria at the phylum level, and Achromobacter, Alcaligenes, and Pseudomonas at the genus level were selectively increased after the electrochemical enrichment culture. Finally, one genus of bacterium that was autotrophically grown on the PEB-carbonate agar was identified as Alcaligenes. This bacterium may be useful to fix atmospheric CO2 with electrochemical energy obtained from the solar cell.
文摘Reductions in CO2 emissions have a significant effect on the transportation sector, and there is increasing interest in developing green cars such as electric cars. To prepare for the advent of the electric car era, it will be necessary to predict the increase in electricity demand owing to the spread of electric cars and determine the policy approaches. Therefore, the analysis was performed to promote the use of electric car that helps reduce CO2 emissions. This study establishes a mode choice model using the stated preference method. To improve the predictive power of the model, some revealed preference data were also examined to consider the characteristics of the commuters and the extent of current electric car technology to determine and verify the parameters of the mode choice models. This was used to estimate changes in CO2 emissions owing to the introduction of electric cars and present effective policy approaches to reduce CO2 emissions.
