To study the immune effect of recombinant avian influenza virus H5 subtype bivalent inactivated vaccine （ HSN1, Re-6 strain ＋ Re-4 strain） and to provide the basis for formulating reasonable immune procedure of avi...To study the immune effect of recombinant avian influenza virus H5 subtype bivalent inactivated vaccine （ HSN1, Re-6 strain ＋ Re-4 strain） and to provide the basis for formulating reasonable immune procedure of avian influenza vaccine in clinical practice. A total of 12 batches of vaccines from three companies were used for the iannune of SPF chickens and SPF ducks. Each chicken or duck serum was separately collected every 3 weeks until the immunization up to the 24^th week. The serum antibody titers of Re-6 and Re-4 were detected. The results showed that the HI titers of the inoculated SPF chickens and SPF ducks roached the peak when the immune time were the 6^th and 3^rd week after the first immunization respectively; then the titer decreased gradually as time prolonged; the highest titer of SPF chickens was greater than that of SPF ducks; the high titer duration of SPF chickens were longer than that of SPF ducks ; and all the vaccines from the three companies showed a good immune effect.展开更多
为了解重组禽流感病毒H5亚型二价灭活疫苗(H5N1,Re-6株+Re-8株)的免疫效果,为临床上制定合理的禽流感疫苗免疫程序提供依据,试验选取14日龄樱桃谷商品肉鸭30只和SPF鸭30只,注射重组禽流感病毒H5亚型二价灭活疫苗(Re-6株+Re-8株)0.4 m L...为了解重组禽流感病毒H5亚型二价灭活疫苗(H5N1,Re-6株+Re-8株)的免疫效果,为临床上制定合理的禽流感疫苗免疫程序提供依据,试验选取14日龄樱桃谷商品肉鸭30只和SPF鸭30只,注射重组禽流感病毒H5亚型二价灭活疫苗(Re-6株+Re-8株)0.4 m L/只作为首次免疫,饲养至28日龄时,樱桃谷肉鸭、SPF鸭随机分成A、B和C、D组,每组均为15只,B、D组再次颈部皮下注射该疫苗0.5 m L/只,均从14日龄起每周采集血清一次,直至56日龄,检测H5抗体情况。结果表明:不同品种鸭对同一亚型禽流感疫苗免疫效果存在差异,首次免疫的日龄可以提至7~10日龄,商品肉鸭一次免疫即可满足防疫需要。展开更多
基金Supported by Shandong Winning Project of Quality Monitoring of Avian Influenza Vaccine
文摘To study the immune effect of recombinant avian influenza virus H5 subtype bivalent inactivated vaccine （ HSN1, Re-6 strain ＋ Re-4 strain） and to provide the basis for formulating reasonable immune procedure of avian influenza vaccine in clinical practice. A total of 12 batches of vaccines from three companies were used for the iannune of SPF chickens and SPF ducks. Each chicken or duck serum was separately collected every 3 weeks until the immunization up to the 24^th week. The serum antibody titers of Re-6 and Re-4 were detected. The results showed that the HI titers of the inoculated SPF chickens and SPF ducks roached the peak when the immune time were the 6^th and 3^rd week after the first immunization respectively; then the titer decreased gradually as time prolonged; the highest titer of SPF chickens was greater than that of SPF ducks; the high titer duration of SPF chickens were longer than that of SPF ducks ; and all the vaccines from the three companies showed a good immune effect.
文摘为了解重组禽流感病毒H5亚型二价灭活疫苗(H5N1,Re-6株+Re-8株)的免疫效果,为临床上制定合理的禽流感疫苗免疫程序提供依据,试验选取14日龄樱桃谷商品肉鸭30只和SPF鸭30只,注射重组禽流感病毒H5亚型二价灭活疫苗(Re-6株+Re-8株)0.4 m L/只作为首次免疫,饲养至28日龄时,樱桃谷肉鸭、SPF鸭随机分成A、B和C、D组,每组均为15只,B、D组再次颈部皮下注射该疫苗0.5 m L/只,均从14日龄起每周采集血清一次,直至56日龄,检测H5抗体情况。结果表明:不同品种鸭对同一亚型禽流感疫苗免疫效果存在差异,首次免疫的日龄可以提至7~10日龄,商品肉鸭一次免疫即可满足防疫需要。
