[Objectives]This study aimed at providing gist for selecting qualified transport containers by comparing the performance of common SPF mouse transport containers at home and abroad,and laying a foundation for subseque...[Objectives]This study aimed at providing gist for selecting qualified transport containers by comparing the performance of common SPF mouse transport containers at home and abroad,and laying a foundation for subsequent improvement of transport containers.[Methods]In this study,5 different transport containers were selected,and their microbial filtration performance,ventilation,sterilization method and flux,and other related design and construction were analyzed.[Results]The results showed all 5 SPF mouse transport containers can provide enough air for the survival of mouse and protect them from bacterial infections.Transport container B and D are superior to others on one-time sterilization flux and deformation degree after autoclaving.Furthermore,taking into account the related design and construction of these transport containers,such as viewing window and carrying handle,the result of comprehensive comparison is that the transport container D>A>E>C>B.[Conclusions]In summary,the 5 SPF transport containers tested in this study can all satisfy the basic demand of SPF mouse transportation,but each transport container has its own advantages and disadvantages on storage space,deformation degree after autoclaving and other aspects.Thus,SPF mouse transport containers should be selected by different demand.展开更多
Phosvitin(PV)was treated with high-temperature,mild pressure(HTMP),and enzyme combination,and then phosvitin phosphopeptides-calcium(PPP-Ca)complexes were prepared.The low-calcium specific pathogen free-Kunming(SPF-KM...Phosvitin(PV)was treated with high-temperature,mild pressure(HTMP),and enzyme combination,and then phosvitin phosphopeptides-calcium(PPP-Ca)complexes were prepared.The low-calcium specific pathogen free-Kunming(SPF-KM)mice were used to determine the effect of PPP-Ca complexes on intestinal calcium absorption and their utilization for bone formation.The serum calcium content was the highest with the HTMP-Enz-PPP-Ca treatment(2.19 mmol/L),and it significantly down-regulated the abnormal elevation of serum alkaline phosphatase(AKP)caused by calcium deficiency.The low-calcium control group had the lowest calcium deposited to the femur(80.41 mg/g)and the lowest femur bone mineral density(BMD)(0.17 g/cm^(3)),while HTMP-Enz-PPP-Ca significantly improved bone calcium content(94.33 mg/g)and BMD(0.29 g/cm^(3)).The micro-computed tomography(MCT)images showed that the femur with the normal control,PV-Ca,and HTMP-Enz-PPP-Ca treatments had a more compact,complete,and thicker trabecular network than the low-calcium and CaCl_(2)treatments.These results indicated that the organic calcium(HTMP-Enz-PPP-Ca)promoted calcium absorption and bone deposition,and the effect of HTMP-Enz-PPP-Ca was better than the inorganic CaCl_(2).展开更多
基金Supported by Scientific Research Project of Jiangsu Laboratory Animal Association(DWXH201907).
文摘[Objectives]This study aimed at providing gist for selecting qualified transport containers by comparing the performance of common SPF mouse transport containers at home and abroad,and laying a foundation for subsequent improvement of transport containers.[Methods]In this study,5 different transport containers were selected,and their microbial filtration performance,ventilation,sterilization method and flux,and other related design and construction were analyzed.[Results]The results showed all 5 SPF mouse transport containers can provide enough air for the survival of mouse and protect them from bacterial infections.Transport container B and D are superior to others on one-time sterilization flux and deformation degree after autoclaving.Furthermore,taking into account the related design and construction of these transport containers,such as viewing window and carrying handle,the result of comprehensive comparison is that the transport container D>A>E>C>B.[Conclusions]In summary,the 5 SPF transport containers tested in this study can all satisfy the basic demand of SPF mouse transportation,but each transport container has its own advantages and disadvantages on storage space,deformation degree after autoclaving and other aspects.Thus,SPF mouse transport containers should be selected by different demand.
基金supported by the General Program of National Natural Science Foundation of China(32072237)the Hubei Provincial Natural Science Foundation of China(2020CFB583)Fundamental Research Funds for the Central Universities(2662020SPPY006)。
文摘Phosvitin(PV)was treated with high-temperature,mild pressure(HTMP),and enzyme combination,and then phosvitin phosphopeptides-calcium(PPP-Ca)complexes were prepared.The low-calcium specific pathogen free-Kunming(SPF-KM)mice were used to determine the effect of PPP-Ca complexes on intestinal calcium absorption and their utilization for bone formation.The serum calcium content was the highest with the HTMP-Enz-PPP-Ca treatment(2.19 mmol/L),and it significantly down-regulated the abnormal elevation of serum alkaline phosphatase(AKP)caused by calcium deficiency.The low-calcium control group had the lowest calcium deposited to the femur(80.41 mg/g)and the lowest femur bone mineral density(BMD)(0.17 g/cm^(3)),while HTMP-Enz-PPP-Ca significantly improved bone calcium content(94.33 mg/g)and BMD(0.29 g/cm^(3)).The micro-computed tomography(MCT)images showed that the femur with the normal control,PV-Ca,and HTMP-Enz-PPP-Ca treatments had a more compact,complete,and thicker trabecular network than the low-calcium and CaCl_(2)treatments.These results indicated that the organic calcium(HTMP-Enz-PPP-Ca)promoted calcium absorption and bone deposition,and the effect of HTMP-Enz-PPP-Ca was better than the inorganic CaCl_(2).
