Objective:To study the influence of over-expression of three functional genes involved in GC biosynthetic pathway,GuHMGR,GuSQS1,and GuBAS on GC production.Methods:Three plant expression vectors were constructed and tr...Objective:To study the influence of over-expression of three functional genes involved in GC biosynthetic pathway,GuHMGR,GuSQS1,and GuBAS on GC production.Methods:Three plant expression vectors were constructed and transformed into Agrobacterium tumefaciens EHA105,which were used to infect Glycyrrhiza uralensis hypocotyls explants.After induction,selection,differentiation,culture,and transplantation,12,15,and 5 regenerated plants over-expressing GuHMGR,GuSQS1,and GuBAS,were obtained,respectively.Results:RT-PCR analysis showed these transgenic regenerated G.uralensis plants had 2-6 copies of GuHMGR,GuSQS1,or GuBAS.HPLC analysis showed the contents of 18α-and 18β-GC in all transgenic regenerated samples were both higher than that in the blank control.With the increase of copy numbers of GuHMGR,GuSQS1,and GuBAS,the contents of 18α-and 18β-GC were both increased in most samples.The highest 18α-and 18β-GC contents in transgenic regenerated plants were about 3.05 times and 2.80 times higher than that in the blank control,respectively.Conclusion:Over-expression of the GuHMGR,GuSQS1,and GuBAS genes enhance the accumulation of 18α-and 18β-GC in the roots and rhizomes of G.uralensis.We hope this work can lay a foundation for the molecular breeding research of G.uralensis and improving the quality of the roots and rhizomes of G.uralensis cultivars.展开更多
目的:建立可用于甘草HMGR,SQS1,β-AS合酶基因CNVs测定的稳定可靠的检测体系。方法:利用Real timePCR方法对甘草HMGR,SQS1,β-AS合酶基因CNVs进行测定。结果:在HMGR,SQS1,β-AS合酶基因以及内标基因lectin的定量检测实验中,其定量反应...目的:建立可用于甘草HMGR,SQS1,β-AS合酶基因CNVs测定的稳定可靠的检测体系。方法:利用Real timePCR方法对甘草HMGR,SQS1,β-AS合酶基因CNVs进行测定。结果:在HMGR,SQS1,β-AS合酶基因以及内标基因lectin的定量检测实验中,其定量反应的Ct值分别在25.82～25.88,29.01～29.08,15.52～15.56,19.06～19.08变化,SD分别是0.033,0.032,0.024,0.011,其CV分别是0.12%,0.22%,0.16%,0.06%。结论:所建立的Real time PCR体系重复性好,检测系统工作稳定可靠,可用于甘草HMGR,SQS1,β-AS基因的CNVs筛选。展开更多
Glycyrrhiza uralensis is frequently used in traditional Chinese medicine. This plant contains a large amount of effective constituents, including triterpenoids and flavonoids. Among them, glycyrrhizin is believed to b...Glycyrrhiza uralensis is frequently used in traditional Chinese medicine. This plant contains a large amount of effective constituents, including triterpenoids and flavonoids. Among them, glycyrrhizin is believed to be the marker compound to evaluate the quality of G. uralensis based on Chinese Pharmacopoeia. Many studies showed that glycyrrhizin possesses various pharmacological activities, such as antibacterial, antiviral, antitumor, anti-inflammatory, and immune-stimulating activities. In this paper, we summarized the cloning, characterization, expression, and polymorphism analysis of several functional genes involved in glycyrrhizin biosynthesis in G. uralensis.展开更多
部分中学教师在阅读课授课时可能会出现过分关注语言知识点而忽略文本本身价值的问题,并且授课模式相对单一,无形之中挫伤了学生学习的积极性与创造性,综合阅读能力迟迟得不到提高。由此,该文主要介绍了SQ3R阅读教学法的具体内容,并以...部分中学教师在阅读课授课时可能会出现过分关注语言知识点而忽略文本本身价值的问题,并且授课模式相对单一,无形之中挫伤了学生学习的积极性与创造性,综合阅读能力迟迟得不到提高。由此,该文主要介绍了SQ3R阅读教学法的具体内容,并以译林版牛津初中《英语》9A Unit 1 People who are happy with their jobs为例设计课堂教学实践。实践证明,SQ3R阅读教学法与阅读课“PWP”模式的结合可以帮助学生掌握阅读技巧,全面提高英语的阅读能力。展开更多
分析病例导入联合行为回放式教学在308nm SQ LED光治疗白癜风教学中的应用效果。方法 选取60名学生作为观察对象,将所有学生按照随机数字表法随机分为对照组和观察组,每组各30名学生。对照组学生给予传统教学,观察组学生给予病例导入联...分析病例导入联合行为回放式教学在308nm SQ LED光治疗白癜风教学中的应用效果。方法 选取60名学生作为观察对象,将所有学生按照随机数字表法随机分为对照组和观察组,每组各30名学生。对照组学生给予传统教学,观察组学生给予病例导入联合行为回放式教学。比较两组学生考核成绩、学习积极性和思维能力评分。结果 经过教学,考核成绩评价显示,观察组学生理论知识(95.42±5.26 vs 85.24±5.46分)、操作技能(90.81±4.25 vs 82.50±4.37分)和沟通技能(92.83±4.85 vs 86.49±4.18分)成绩均高于对照组学生(P<0.05)。观察组学生学习积极性(8.56±1.04 vs 7.06±1.26分)和思维能力评分(8.79±0.92 vs 7.23±1.06分)均高于对照组学生(P<0.05)。结论 病例导入联合行为回放式教学在308nm SQ LED光治疗白癜风教学中的应用效果良好,能够提高学生的考核成绩,提高学生的学生积极性和思维能力。展开更多
文摘Objective:To study the influence of over-expression of three functional genes involved in GC biosynthetic pathway,GuHMGR,GuSQS1,and GuBAS on GC production.Methods:Three plant expression vectors were constructed and transformed into Agrobacterium tumefaciens EHA105,which were used to infect Glycyrrhiza uralensis hypocotyls explants.After induction,selection,differentiation,culture,and transplantation,12,15,and 5 regenerated plants over-expressing GuHMGR,GuSQS1,and GuBAS,were obtained,respectively.Results:RT-PCR analysis showed these transgenic regenerated G.uralensis plants had 2-6 copies of GuHMGR,GuSQS1,or GuBAS.HPLC analysis showed the contents of 18α-and 18β-GC in all transgenic regenerated samples were both higher than that in the blank control.With the increase of copy numbers of GuHMGR,GuSQS1,and GuBAS,the contents of 18α-and 18β-GC were both increased in most samples.The highest 18α-and 18β-GC contents in transgenic regenerated plants were about 3.05 times and 2.80 times higher than that in the blank control,respectively.Conclusion:Over-expression of the GuHMGR,GuSQS1,and GuBAS genes enhance the accumulation of 18α-and 18β-GC in the roots and rhizomes of G.uralensis.We hope this work can lay a foundation for the molecular breeding research of G.uralensis and improving the quality of the roots and rhizomes of G.uralensis cultivars.
文摘目的:建立可用于甘草HMGR,SQS1,β-AS合酶基因CNVs测定的稳定可靠的检测体系。方法:利用Real timePCR方法对甘草HMGR,SQS1,β-AS合酶基因CNVs进行测定。结果:在HMGR,SQS1,β-AS合酶基因以及内标基因lectin的定量检测实验中,其定量反应的Ct值分别在25.82～25.88,29.01～29.08,15.52～15.56,19.06～19.08变化,SD分别是0.033,0.032,0.024,0.011,其CV分别是0.12%,0.22%,0.16%,0.06%。结论:所建立的Real time PCR体系重复性好,检测系统工作稳定可靠,可用于甘草HMGR,SQS1,β-AS基因的CNVs筛选。
基金Beijing Project for Young Talents(YETP0819)National Science Foundation of China(81503181)
文摘Glycyrrhiza uralensis is frequently used in traditional Chinese medicine. This plant contains a large amount of effective constituents, including triterpenoids and flavonoids. Among them, glycyrrhizin is believed to be the marker compound to evaluate the quality of G. uralensis based on Chinese Pharmacopoeia. Many studies showed that glycyrrhizin possesses various pharmacological activities, such as antibacterial, antiviral, antitumor, anti-inflammatory, and immune-stimulating activities. In this paper, we summarized the cloning, characterization, expression, and polymorphism analysis of several functional genes involved in glycyrrhizin biosynthesis in G. uralensis.
文摘部分中学教师在阅读课授课时可能会出现过分关注语言知识点而忽略文本本身价值的问题,并且授课模式相对单一,无形之中挫伤了学生学习的积极性与创造性,综合阅读能力迟迟得不到提高。由此,该文主要介绍了SQ3R阅读教学法的具体内容,并以译林版牛津初中《英语》9A Unit 1 People who are happy with their jobs为例设计课堂教学实践。实践证明,SQ3R阅读教学法与阅读课“PWP”模式的结合可以帮助学生掌握阅读技巧,全面提高英语的阅读能力。
文摘分析病例导入联合行为回放式教学在308nm SQ LED光治疗白癜风教学中的应用效果。方法 选取60名学生作为观察对象,将所有学生按照随机数字表法随机分为对照组和观察组,每组各30名学生。对照组学生给予传统教学,观察组学生给予病例导入联合行为回放式教学。比较两组学生考核成绩、学习积极性和思维能力评分。结果 经过教学,考核成绩评价显示,观察组学生理论知识(95.42±5.26 vs 85.24±5.46分)、操作技能(90.81±4.25 vs 82.50±4.37分)和沟通技能(92.83±4.85 vs 86.49±4.18分)成绩均高于对照组学生(P<0.05)。观察组学生学习积极性(8.56±1.04 vs 7.06±1.26分)和思维能力评分(8.79±0.92 vs 7.23±1.06分)均高于对照组学生(P<0.05)。结论 病例导入联合行为回放式教学在308nm SQ LED光治疗白癜风教学中的应用效果良好,能够提高学生的考核成绩,提高学生的学生积极性和思维能力。
