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骨质疏松分子生物学研究专家共识
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作者 《中国骨质疏松杂志》社 《中国骨质疏松杂志》骨代谢专家组 +2 位作者 张萌萌 毛未贤 马倩倩 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第2期157-162,共6页
骨质疏松分子生物学研究在骨代谢分子信号通路、骨质疏松易感基因、骨质疏松相关蛋白、骨质疏松靶向治疗等方向取得了很大进展。核因子κB受体活化因子/核因子κB受体活化因子配体/骨保护素(RANK/RANKL/OPG)信号通路、核因子κB受体活... 骨质疏松分子生物学研究在骨代谢分子信号通路、骨质疏松易感基因、骨质疏松相关蛋白、骨质疏松靶向治疗等方向取得了很大进展。核因子κB受体活化因子/核因子κB受体活化因子配体/骨保护素(RANK/RANKL/OPG)信号通路、核因子κB受体活化因子(NF-κB)信号通路、丝裂原活化蛋白激酶/细胞外调节蛋白激酶(MAPK/ERK)信号通路、巨噬细胞集落刺激因子(M-CSF)信号通路、钙离子(Ca^(2+))信号通路、酪氨酸激酶、蛋白激酶B(Src、Akt)信号通路、蛋白激酶C(PKC)信号通路等骨代谢重要通路,维生素D受体(VDR)基因、低密度脂蛋白相关蛋白5(LRP5)基因、亚甲基四氢叶酸还原酶(MTHFR)基因、雌激素受体(ER)基因等易感基因,载脂蛋白E(Apo E)、Klotho蛋白(Klotho)、骨形态发生蛋白(BMP)、骨涎蛋白(BSP)等相关蛋白,以直接或间接的方式参与调控骨代谢,作用重叠相互联系,互为结果,已在本专业领域达成共识。 展开更多
关键词 κB/κB/ κB / B C Wnt/β- Hedghog 2/Smad /B D 5 α1α2 E Klotho 5 -1
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Numerical Simulation of Response of SRC Columns Subjected to Blast Loading 被引量:6
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作者 SUN Jianyun LI Guoqiang LU Yong 《Transactions of Tianjin University》 EI CAS 2006年第B09期126-131,共6页
The dynamic characteristics and failure modes of steel reinforced concrete (SRC) columns subjected to blast loading are complicated because of the transient stress wave in the SRC columns and the interaction between s... The dynamic characteristics and failure modes of steel reinforced concrete (SRC) columns subjected to blast loading are complicated because of the transient stress wave in the SRC columns and the interaction between steel and concrete. This paper presents a numerical simulation of the response of SRC columns subjected to blast loading using hydrocode LS-DYNA. In the numerical model, a sophisticate concrete material model (the Concrete Damage Model) is employed with consideration of the strain rate effect and the damage accumulation. An erosion technique is adopted to model the spalling process of concrete. The possible failure modes of SRC columns are evaluated. It is observed that the failure of SRC columns subjected to blast load can generally be classified into three modes, namely, a direct failure in concrete body due to the stress wave, a transverse shear failure near the support sections due to the high shear force, and a flexural failure pertaining to large local and global deformation of the reinforcing steel. 展开更多
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鞣花酸通过TLR4-SRC/MAPK/NF-κB途径抑制脂多糖诱导RAW264.7巨噬细胞的炎症反应
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作者 乌里盼·托乎达阿里 丁宛婷 +3 位作者 孙媛 周茂杰 姚雨含 赵军 《中南药学》 CAS 2024年第4期943-949,共7页
目的基于Toll样受体4(TLR4)-酪氨酸激酶(SRC)/丝裂原活化蛋白激酶(MAPK)/核转录因子-κB(NF-κB)通路探讨鞣花酸的抗炎机制。方法采用不同浓度(0、0.5、5、25、50、75、100μmol·L^(-1))鞣花酸对RAW264.7巨噬细胞[或脂多糖(LPS)诱... 目的基于Toll样受体4(TLR4)-酪氨酸激酶(SRC)/丝裂原活化蛋白激酶(MAPK)/核转录因子-κB(NF-κB)通路探讨鞣花酸的抗炎机制。方法采用不同浓度(0、0.5、5、25、50、75、100μmol·L^(-1))鞣花酸对RAW264.7巨噬细胞[或脂多糖(LPS)诱导的RAW264.7细胞]干预24 h,噻唑蓝(MTT)法检测RAW264.7巨噬细胞存活率,筛选最适给药浓度。实验分为空白对照组,模型组(0.5 mg·L^(-1)LPS),阳性对照地塞米松组(10μmol·L^(-1)),鞣花酸(5、25、50μmol·L^(-1))给药组。Griess法检测细胞上清液中一氧化氮(NO)的含量;采用ELISA法检测细胞上清液中前列腺素-2(PGE2)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的表达水平;采用Western blot法检测诱导型一氧化氮合酶(iNOS)、环氧合酶-2(COX-2)蛋白以及TLR4-SRC/MAPK/NF-κB通路相关蛋白的表达水平。结果MTT结果筛选出鞣花酸的干预浓度为5~50μmol·L^(-1)。与空白对照组比较,模型组PGE_(2)、NO和炎症因子IL-6、IL-1β、TNF-α表达显著升高(P<0.01);炎症标志物iNOS、COX-2蛋白表达水平显著升高(P<0.01),说明炎症模型建立成功。与模型组比较,鞣花酸能显著抑制LPS诱导NO、PGE_(2)的产生和降低TNF-α、IL-1β、IL-6水平(P<0.05,P<0.01),降低i NOS、COX-2蛋白表达水平(P<0.05,P<0.01),且呈浓度依赖性;显著抑制TLR4蛋白的表达水平以及SRC、P38、JNK、p65、IκBα蛋白的磷酸化水平,并呈浓度依赖性。但对ERK1/2蛋白磷酸化没有显示出明显的抑制作用。结论鞣花酸可能通过TLR4-SRC/MAPK/NF-κB信号通路的调控抑制LPS诱导RAW264.7巨噬细胞的炎症反应,具体的调控机制有待进一步研究。 展开更多
关键词 RAW264.7 src蛋白激酶 MAPK NF-ΚB
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Methanol Distillation System: Process Analysis and Column Design
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作者 Sun Jinsheng Tian Yufeng Xu Shimin Ding Hui Wang Tao Li Xingang Zheng Yanmei 《化工进展》 EI CAS CSCD 北大核心 2005年第z1期40-51,共12页
Base on industrial research and experience, the process of methanol distillation is analyzed,and above all, a new concept of high pressure flowsheet and low pressure flowsheet is defined. The new configuration helps t... Base on industrial research and experience, the process of methanol distillation is analyzed,and above all, a new concept of high pressure flowsheet and low pressure flowsheet is defined. The new configuration helps to handle problems encountered in many factories in China. The inter influence between process and column internal pattern is also pointed out. Recommendation of new column internal designs is given. Finally, industrial examples tell the how the new concept works and the possibility of combining process to give more opens to solve engineering problems. 展开更多
关键词 METHANOL distillation two - effect distillation high PRESSURE flowsheet and low PRESSURE flowsheet flowsheet analysis simulation with hydraulic column INTERNAL design
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黄土高原SRC参数的空间物理释义与流量估算应用
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作者 薛超伟 张洪波 +4 位作者 冶兆霞 张雨柔 杨志芳 赵孝威 李同方 《人民黄河》 CAS 北大核心 2024年第4期23-31,共9页
黄土高原地处我国西北旱区,降水稀少且潜在蒸发强烈,包气带较厚且空间异质性大等多种问题交织耦合导致区域河川径流演化机制极其复杂,影响区域生态保护与高质量发展的进程。对黄土高原不同生态分区的10个代表性流域的SRC参数进行空间分... 黄土高原地处我国西北旱区,降水稀少且潜在蒸发强烈,包气带较厚且空间异质性大等多种问题交织耦合导致区域河川径流演化机制极其复杂,影响区域生态保护与高质量发展的进程。对黄土高原不同生态分区的10个代表性流域的SRC参数进行空间分析,研究了SRC参数的异质性分布规律,探析了SRC参数在黄土高原地区的物理释义,并基于SRC建立了流域出口断面流量与陆地水储量的区域化响应关系,实现了通过GRACE重力卫星反演数据对流域出口断面流量的初步估算。研究表明,同一生态区内SRC参数α、β具有一定的稳定性,其中β和流域的干旱指数成负相关关系,与平均坡度成正相关关系,在地理位置中随纬度的减小和经度的增大而增大;而α则和土壤孔隙度存在正相关关系,与导水率衰减参数存在负相关关系。通过幂函数可建立黄土高原流域陆地水储量与出口断面流量间的拟合关系,并形成适用于平稳GRACE数据的流域流量过程估算方法。 展开更多
关键词 src GRACE
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用于电梯曳引机减振的MR-SRC减振器设计与分析
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作者 任志英 刘荣阳 +3 位作者 黄伟 李成威 史林炜 徐彩军 《振动与冲击》 EI CSCD 北大核心 2024年第3期295-304,共10页
针对现有曳引机减振常采用的高分子减振垫因环境因素而引发性能大幅退化问题,以电梯曳引机减振器的工程应用需求为导向,确定减振器所需阻尼元件的刚度后,采用真空渗流技术将硅橡胶填充入金属橡胶孔隙并形成包覆,研制出一款耐环境影响的... 针对现有曳引机减振常采用的高分子减振垫因环境因素而引发性能大幅退化问题,以电梯曳引机减振器的工程应用需求为导向,确定减振器所需阻尼元件的刚度后,采用真空渗流技术将硅橡胶填充入金属橡胶孔隙并形成包覆,研制出一款耐环境影响的高性能金属橡胶/硅橡胶连续互穿相复合减振材料(metal rubber-silicone rubber continuous interwoven phase composite damping material, MR-SRC)。然后基于实际结构建立电梯曳引机的有限元数值模型。对加装MR-SRC减振器前后的电梯曳引机进行了模态分析,确定了整体结构的固有频率和振型。对MR-SRC减振器进行冲击响应分析,对比不同MR-SRC材料和冲击载荷对响应的影响,校核减振器的强度。接着,基于MR-SRC的近似等效,对减振器系统的冲击响应进行了理论计算,得出金属橡胶密度为1.8 g/cm^(3)的MR-SRC减振器对减振效果最佳。最后将所制备的MR-SRC减振器安装于实际电梯曳引机,按照电梯减振国标要求,测试电梯在运行过程中的加速度和速度变化,计算并校核电梯运行的相关特性参数。该文章提出的减振器为电梯安全可靠运行提供了一种新途径。 展开更多
关键词 -线
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Seismic cyclic loading test of SRC columns confined with 5-spirals 被引量:5
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作者 WENG ChengChiang YIN YenLiang +1 位作者 WANG JuiChen LIANG ChingYu 《Science China(Technological Sciences)》 SCIE EI CAS 2008年第5期529-555,共27页
Presented herein is an experimental study on seismic resistance of rectangular steel reinforced concrete (SRC) columns confined with a new type of multi-spiral cage. The multi-spiral cage is a device of five interconn... Presented herein is an experimental study on seismic resistance of rectangular steel reinforced concrete (SRC) columns confined with a new type of multi-spiral cage. The multi-spiral cage is a device of five interconnected spirals, named "5-spirals", with a large spiral at the center and four small ones at the corners. The innovation of applying the 5-spirals to SRC column is to take its superiority in concrete confinement and efficiency in automatic production for the precast construction industry. Four full-scale SRC columns were tested under horizontal cyclic loading. All of the tested columns were capable of sustaining a drift angle up to 6% radians. The hysteresis loops observed from the cyclic loading tests indicated that the spirally confined SRC columns demonstrated excellent performances in both strength and ductility. The test results suggested that with significant saving of the confinement steel, the newly innovated 5-spirals can be successfully applied to the precast rectangular SRC columns. 展开更多
关键词 steel reinforced CONCRETE rectangular src column PRECAST construction 5-spirals CONCRETE CONFINEMENT cyclic loading test SEISMIC resistance
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LIMK1通过上调ROS/Src通路进而促进宫颈癌的进展
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作者 贾亚静 杜耐一 +2 位作者 赵炜 李永萍 刘雅坤 《中国比较医学杂志》 CAS 北大核心 2024年第5期92-99,共8页
目的探究LIMK1对宫颈癌(cervical cancer)进展的影响。方法构建LIMK1过表达人宫颈癌HeLa细胞及C-33A细胞,将HeLa细胞接种于裸鼠皮下,观察肿瘤体积并以Western blot实验检测其瘤体细胞中NOX2、NOX4、p-Src、p-RUNX3、RUNX3以及MMP-9蛋白... 目的探究LIMK1对宫颈癌(cervical cancer)进展的影响。方法构建LIMK1过表达人宫颈癌HeLa细胞及C-33A细胞,将HeLa细胞接种于裸鼠皮下,观察肿瘤体积并以Western blot实验检测其瘤体细胞中NOX2、NOX4、p-Src、p-RUNX3、RUNX3以及MMP-9蛋白表达情况;将LIMK1过表达HeLa细胞及LIMK1过表达C-33A细胞培养于5%O 2条件下,并加入抗氧化剂,以Western blot实验检测细胞内LIMK1、NOX4、p-Src、p-RUNX3、RUNX3以及MMP-9蛋白表达情况,以划痕实验检测细胞迁移能力,以Transwell实验检测细胞迁移和侵袭能力,以单克隆增殖实验检测细胞增殖能力。结果接种LIMK1过表达HeLa细胞的裸鼠肿瘤体积明显增大,其NOX2、NOX4、p-Src、p-RUNX3以及MMP-9蛋白升高,而RUNX3蛋白表达降低;LIMK1过表达的HeLa细胞LIMK1、NOX4、p-Src、p-RUNX3以及MMP-9蛋白表达升高,RUNX3蛋白表达降低,同时LIMK1过表达的HeLa细胞及LIMK1过表达C-33A细胞的迁移和侵袭能力以及细胞增殖能力增强,而加入抗氧化剂后,细胞的NOX4、p-Src、p-RUNX3、RUNX3以及MMP-9蛋白表达水平以及细胞迁移、侵袭和增殖能力与LIMK1正常表达的细胞无显著差异。结论LIMK1通过促进ROS/Src通路进而促进宫颈癌细胞的迁移、侵袭和增殖能力,促进了宫颈癌的进展。 展开更多
关键词 LIMK1 ROS src RUNX3
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钢框架-自复位SRC墙板结构的地震易损性分析
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作者 隋璐 吴函恒 +1 位作者 唐鹏辉 袁涛涛 《建筑钢结构进展》 CSCD 北大核心 2024年第1期10-21,共12页
为了研究钢框架-型钢混凝土(SRC)自复位墙板结构的抗震性能,完成了钢框架-自复位SRC墙板子结构模型的低周往复加载试验,以研究其工作机理和失效机制。提出了基于宏观单元的非线性分析模型,并利用试验结果验证模型的准确性,研究结果表明... 为了研究钢框架-型钢混凝土(SRC)自复位墙板结构的抗震性能,完成了钢框架-自复位SRC墙板子结构模型的低周往复加载试验,以研究其工作机理和失效机制。提出了基于宏观单元的非线性分析模型,并利用试验结果验证模型的准确性,研究结果表明该非线性分析模型能较好地模拟自复位SRC墙板的非线性行为。分别建立6层和15层钢框架-自复位SRC墙板结构的整体模型并进行增量动力时程分析(IDA)。以地面峰值加速度为地震动强度指标,以最大层间位移角为损伤指标,根据IDA结果得到不同极限状态下的易损性曲线,建立地震易损性矩阵,并对不同极限状态下结构的损伤风险进行了评估,研究表明:6层和15层钢框架-自复位SRC墙板结构在50年内的倒塌概率分别为0.138%和0.095%,远小于FEMA 750规定的1%,由此可见,钢框架-自复位SRC墙板结构抗震性能较好,并具备良好的抗地震倒塌能力。 展开更多
关键词 自复位src墙板 线
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参七虫草方通过ASS1/src/STAT3信号通路改善肺纤维化大鼠的炎症反应
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作者 何程 陈炜 +3 位作者 张念志 栾军 王三凤 张尤 《南方医科大学学报》 CAS CSCD 北大核心 2024年第4期644-651,共8页
目的观察参七虫草方对肺纤维化大鼠精氨酸琥珀酸盐合成酶-1(ASS1)/src酪氨酸激酶(src)/信号转导和转化激活因子3(STAT3)通路相关因子表达的影响,探讨其治疗肺纤维化的作用机制。方法选择120只SPF级SD雄性大鼠,采用随机数字表法分为5组:... 目的观察参七虫草方对肺纤维化大鼠精氨酸琥珀酸盐合成酶-1(ASS1)/src酪氨酸激酶(src)/信号转导和转化激活因子3(STAT3)通路相关因子表达的影响,探讨其治疗肺纤维化的作用机制。方法选择120只SPF级SD雄性大鼠,采用随机数字表法分为5组:空白组、模型组、参七虫草方组、吡非尼酮组、精氨酸脱亚胺酶(ADI)腹腔注射组(24只/组)。除空白组以外,4组均使用一次性气管内滴注博来霉素(BLM)诱导制备特发性肺纤维化的大鼠模型。造模成功后,参七虫草组予参七虫草方汤剂(0.423 g/kg)灌胃;吡非尼酮组予溶于生理盐水的吡非尼酮胶囊粉末(10 mL·kg^(-1)·d^(-1))灌胃2次;ADI腹腔注射组予以2.25 mg/(kg·d)腹腔注射,1次/3d;空白组、模型组分别以10mL/(kg·d)生理盐水灌胃;给药疗程28d。于第7、14、21、28天分4批处死大鼠,分离肺组织并计算肺指数。采用苏木素-伊红、马松染色观察大鼠肺组织的组织病理学;吉姆萨染色分析BALF中不同种类的炎症细胞;采用酶联免疫吸附测定法检测血清趋化因子配体2(CCL2)、转化生长因子β1(TGF-β1)的含量;蛋白免疫印迹法测定肺组织src、p-src^(Try529)、STAT3、p-STAT3^(Try705)的蛋白表达;采用实时荧光定量聚合酶链式反应技术检测肺组织ASS1、src、STAT3的表达水平。结果参七虫草组、吡非尼酮组和ADI腹腔注射组各时间点中性粒细胞、巨噬细胞、淋巴细胞均低于同一时间点的模型组(P<0.05)。在相同时间点,参七虫草组、吡非尼酮组和ADI腹腔注射组大鼠血清CCL2、TGF-β1水平均低于模型组(P<0.05)。参七虫草组、吡非尼酮组和ADI腹腔注射组的p-src^(Try529)、p-STAT3^(Try705)蛋白表达水平及肺组织ASS1、src、STAT3mRNA均低于同一时间点的模型组(P<0.05)。结论参七虫草方可以缓解大鼠的肺纤维化程度,其机制可能通过调控ASS1/src/STAT3信号通路的活化从而缓解BLM大鼠肺纤维化的炎症反应。 展开更多
关键词 ASS1/src/STAT3信号通路
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长周期地震动对SRC框架-RC核心筒混合结构协同工作的影响
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作者 杨柯 王博 +2 位作者 祝超 刘伯权 李红 《振动与冲击》 EI CSCD 北大核心 2024年第12期36-45,共10页
长周期地震动因含有丰富的低频成分易使(超)高层建筑等长周期结构发生严重震害。SRC(steel reinforced concrete)框架-RC(reinforced concrete)核心筒混合结构作为广泛应用于高烈度区的双重抗侧力结构体系,确保其强震下的协同工作是实... 长周期地震动因含有丰富的低频成分易使(超)高层建筑等长周期结构发生严重震害。SRC(steel reinforced concrete)框架-RC(reinforced concrete)核心筒混合结构作为广泛应用于高烈度区的双重抗侧力结构体系,确保其强震下的协同工作是实现多道抗震防线,形成合理破坏模式的关键。该文通过与普通地震动对比,阐明了远场长周期地震动对SRC框架-RC核心筒混合结构协同工作的影响规律,并基于谐振激励下的结构动力分析模型及经验模态分解初步揭示了其内在机理。算例分析结果表明:受低频特性影响,远场长周期地震动作用下结构框架剪力分担率与底层倾覆力矩占比明显大于普通地震动,最大框架剪力分担率所在楼层与普通地震动相比出现下移,且当峰值加速度增至400 gal时,SRC框架中下部楼层剪力分担率超过40%,底部倾覆力矩占比超过70%,按照现行规范设计的SRC框架将难以保证远场长周期地震动作用下结构的抗震安全;激励频率对于SRC框架-RC核心筒混合结构协同工作的影响与振型参与方式有关,低频激励(周期为0.5倍~2.0倍结构基本周期)下结构响应主要受低阶振型主导;远场长周期地震动的卓越分量是导致框架剪力分担率增大的原因。 展开更多
关键词 src框架-RC核心筒混合结构 -
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武汉地区湖泊沉积物重金属的分布及潜在生态效应评价 被引量:77
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作者 乔胜英 蒋敬业 +1 位作者 向武 唐俊红 《长江流域资源与环境》 CAS CSSCI CSCD 北大核心 2005年第3期353-357,共5页
对武汉地区6个湖泊沉积物重金属元素Hg、Cd、Cu、Pb、Zn、As、Cr、Ni的空间分布特征及其潜在生态效应进行研究。调查区的湖泊底泥重金属含量表现出市区内湖泊沉积物的重金属含量普遍高于郊区湖泊,其中墨水湖中重金属含量最高;湖泊沉积... 对武汉地区6个湖泊沉积物重金属元素Hg、Cd、Cu、Pb、Zn、As、Cr、Ni的空间分布特征及其潜在生态效应进行研究。调查区的湖泊底泥重金属含量表现出市区内湖泊沉积物的重金属含量普遍高于郊区湖泊,其中墨水湖中重金属含量最高;湖泊沉积物柱重金属元素垂向分布表现为城区受到一定程度污染的湖泊某些元素表现出表层沉积物显著高于底部沉积物含量的特征,而郊区湖泊除Cd外其它元素垂向含量变化不大,说明郊区湖泊受到人为影响较小。利用潜在生态危害指数法对湖泊沉积物进行生态危害评价,显示武汉地区湖泊元素生态危害排序为:Cd>Hg>As>Cu>Pb>Zn;墨水湖沉积物重金属潜在生态危害相对最高,其次是金银湖;其它湖泊重金属生态危害都比较轻,总体而言武汉湖泊重金属生态危害还是较小的。但参照国外沉积物基准的生态数据库阈值,市区受到人为污染较严重的湖泊沉积物可能会对生物产生负面影响。 展开更多
关键词 Cd Hg Zn As
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微小RNA-150及靶基因SRC激酶信号抑制剂1在下肢深静脉血栓中的表达及作用机制
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作者 李孝成 陈俞宏 韦征霞 《血管与腔内血管外科杂志》 2024年第3期338-344,共7页
目的探讨微小RNA(miRNA)-150及靶基因SRC激酶信号抑制剂1(SRCIN1)在下肢深静脉血栓(LEDVT)中的表达及作用机制。方法收集2022年1月至2023年1月柳州市人民医院收治的60例LEDVT患者的临床资料作为LEDVT组,另纳入同期进行体检的60例健康者... 目的探讨微小RNA(miRNA)-150及靶基因SRC激酶信号抑制剂1(SRCIN1)在下肢深静脉血栓(LEDVT)中的表达及作用机制。方法收集2022年1月至2023年1月柳州市人民医院收治的60例LEDVT患者的临床资料作为LEDVT组,另纳入同期进行体检的60例健康者作为对照组。采集两组受试者的外周静脉血,并分离内皮祖细胞。通过荧光定量逆转录聚合酶链反应(qRT-PCR)检测miRNA-150的表达水平,利用生物信息学分析和双荧光素酶报告基因实验验证miRNA-150的靶基因,采用蛋白质印迹法(Western blot)检测SRCIN1蛋白的表达,采用四甲基偶氮唑蓝(MTT)实验检测内皮祖细胞增殖情况。结果与对照组相比,LEDVT组内皮祖细胞中miRNA-150的相对表达量明显降低(P﹤0.01)。生物信息学分析和双荧光素酶报告基因实验证实SRCIN1是miRNA-150的靶基因;在野生型SRCIN1中,与转染miRNA-NC的细胞相比,转染miRNA-150 agomir细胞的荧光素酶活性明显降低(P﹤0.01)。在突变型SRCIN1中,miRNA-150对SRCIN1的负向调控作用消失。Western blot检测结果显示,与miRNA-150antagomir组内皮祖细胞相比,miRNA-150 agomir组内皮祖细胞中SRCIN1蛋白的表达水平降低(P﹤0.05);与对照组相比,LDVT组内皮祖细胞中SRCIN1蛋白的表达水平升高(P﹤0.05)。MTT实验检测结果显示,与miRNA-150antagomir组相比,miRNA-150 agomir组的吸光度(OD)值在48、72 h时均明显升高(P﹤0.01)。结论miRNA-150在内皮祖细胞中的表达水平降低可能与LEDVT的发生、发展有关。高表达的miRNA-150可能通过靶向SRCIN1促进内皮祖细胞的增殖,从而达到治疗LEDVT的目的。 展开更多
关键词 RNA-150 src激酶信号抑制剂1
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Seismic behavior of large-scale FRP–recycled aggregate concrete–steel columns with shear connectors 被引量:2
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作者 Zeng Lan Li Lijuan +1 位作者 Yang Xianqian Liu Feng 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2019年第4期823-844,共22页
The application of fi ber-reinforced polymer (FRP) composites for the development of high-performance composite structural systems has received signifi cant recent research attention. A composite of FRP–recycled aggr... The application of fi ber-reinforced polymer (FRP) composites for the development of high-performance composite structural systems has received signifi cant recent research attention. A composite of FRP–recycled aggregate concrete (RAC)–steel column (FRSC), consisting of an outer FRP tube, an inner steel tube and annular RAC fi lled between two tubes, is proposed herein to facilitate green disposal of demolished concrete and to improve the ductility of concrete columns for earthquake resistance. To better understand the seismic behavior of FRSCs, quasi-static tests of large-scale basalt FRSCs with shear connectors were conducted. The infl uence of the recycled coarse aggregate (RCA) replacement percentage, shear connectors and axial loading method on the lateral load and deformation capacity, energy dissipation and cumulative damage were analyzed to evaluate the seismic behavior of FRSCs. The test results show that FRSCs have good seismic behavior, which was evidenced by high lateral loads, excellent ductility and energy dissipation capacity, indicating RAC is applicable in FRSCs. Shear connectors can signifi cantly postpone the steel buckling and increase the lateral loads of FRSCs, but weaken the deformation capacity and energy dissipation performance. 展开更多
关键词 recycled AGGREGATE concrete (RAC) fi ber-reinforced polymer (FRP) FRPRACsteel column (FRSC) shear connector SEISMIC behavior
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柱切换直接进样高效液相色谱法测定蜂蜜中3种抗生素的残留量 被引量:12
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作者 薄海波 柳春辉 +2 位作者 张红丽 李永民 陈立仁 《分析化学》 SCIE EI CAS CSCD 北大核心 2005年第4期515-518,共4页
建立了柱切换反相高效液相色谱法直接进样分离、测定蜂蜜中3种四环素族抗生素(土霉素OTC、四环素TC、金霉素CTC)残留量的分析方法.方法包括:用缓冲溶液溶解样品、直接进样、二次蒸馏水作流动相在C18预柱上在线富集和净化,然后用柱切换... 建立了柱切换反相高效液相色谱法直接进样分离、测定蜂蜜中3种四环素族抗生素(土霉素OTC、四环素TC、金霉素CTC)残留量的分析方法.方法包括:用缓冲溶液溶解样品、直接进样、二次蒸馏水作流动相在C18预柱上在线富集和净化,然后用柱切换阀将预柱与一个C18分析柱接通,草酸溶液-乙腈-甲醇作流动相、紫外检测器在350 nm处检测.各组分回收率均大于85%;相对标准偏差小于10%;标准曲线的相关系数在0.9983~0.9991之间;最低检出浓度(≤0.02 mg/kg),满足欧盟和日本等国要求(0.05mg/kg). 展开更多
关键词 线 线 C18 OTC
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Achievements of Truss Models for Reinforced Concrete Structures 被引量:2
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作者 P. G. Papadopoulos H. Xenidis +3 位作者 P. Lazaridis A. Diamantopoulos P. Lambrou Y. Arethas 《Open Journal of Civil Engineering》 2012年第3期125-131,共7页
Achievements are presented for truss models of RC structures developed in previous years: 1. Two constitutive models, biaxial and triaxial, are based on regular trusses, with bars obeying nonlinear uniaxial σ-ε laws... Achievements are presented for truss models of RC structures developed in previous years: 1. Two constitutive models, biaxial and triaxial, are based on regular trusses, with bars obeying nonlinear uniaxial σ-ε laws of material under simulation;both models have been compared with test results and show a dependence of Poisson ratio on curvature of σ-ε law. 2. A truss finite element has been used in the nonlinear static and dynamic analysis of plane RC frames;it has been compared with test results and describes, in a simple way, the formation of plastic hinges. 3. Thanks to the very simple geometry of a truss, the equilibrium equations can be easily written and the stiffness matrix can be easily updated, both with respect to the deformed truss, within each step of a static incremental loading or within each time step of a dynamic analysis, so that to take into account geometric nonlinearities. So the confinement of a RC column is interpreted as a structural stability effect of concrete. And a significant role of the transverse reinforcement is revealed, that of preventing, by its close spacing and sufficient amount, the buckling of inner longitudinal concrete struts, which would lead to a global instability of the RC column. 4. The proposed truss model is statically indeterminate, so it exhibits some features, which are not met by the “strut-and-tie” model. 展开更多
关键词 Reinforced CONCRETE Structure TRUSS Model CONSTITUTIVE Law Material and Geometric NONLINEARITIES CONCRETE Cracking Reinforcement Yield CONCRETE ULTIMATE Compressive Strength Plastic HINGE RC column Confinement Buckling of Inner CONCRETE Struts Global Instability
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Hydrodynamics of air-kerosene bubble column under elevated pressure in homogeneous flow regime 被引量:1
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作者 Bay Van Tran Son Ich Ngo +4 位作者 Young-Il Lim Keon Bae Dong Hyun Lee Kang-Seok Go Nam-Sun Nho 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2021年第5期190-202,共13页
A multiphase computational fluid dynamics(CFD)model coupled with the population balance equation(PBE)was developed in a homogeneous air-kerosene bubble column under elevated pressure(P).The specific pressure drop(ΔP/... A multiphase computational fluid dynamics(CFD)model coupled with the population balance equation(PBE)was developed in a homogeneous air-kerosene bubble column under elevated pressure(P).The specific pressure drop(ΔP/L),gas holdup(α_(G)),and Sauter mean diameter(d_(32))were experimentally measured in the bubble column with 1.8 m height and 0.1 m inner diameter,which was operated at a superficial gas velocity of 12.3 mm·s^(-1),and P=1-35 bar(1 bar=10^(5) Pa).A modified drag coefficient model was proposed to consider the effect of bubble swarm and pressure on hydrodynamics of the bubble column.The Luo breakage model was modified to account for liquid density,viscosity,surface tension and gas density.TheΔP/L,α_(G),and d_(32) obtained from the CFD model were compared with experimental data,and the gas density-dependent parameters of the CFD model were identified.With increasing P from 1 to 35 bar,theα_(G) varied from 5.4%to 7.2% and the d32 decreased from 2.3 to 1.5 mm.The CFD-PBE model is applicable to predict hydrodynamics of pressurized bubble columns for gas-organic liquid in the homogeneous regime. 展开更多
关键词 BUBBLE column Air-kerosene High pressure HOMOGENOUS regime Computational fluid dynamics (CFD) Population balance equation (PBE)
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Modeling bubble column reactor with the volume of fluid approach:Comparison of surface tension models 被引量:4
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作者 Qi Liu Zhenghong Luo 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2019年第11期2659-2665,共7页
This work aims at comparing surface tension models in VOF(Volume of Fluid) modeling and investigating the effects of gas distributor and gas velocity. Hydrodynamics of a continuous chain of bubbles inside a bubble col... This work aims at comparing surface tension models in VOF(Volume of Fluid) modeling and investigating the effects of gas distributor and gas velocity. Hydrodynamics of a continuous chain of bubbles inside a bubble column reactor was simulated. The grid independence study was first conducted and a grid size of 1.0 mm was adopted in order to minimize the computing time without compromising the accuracy of the results. The predictions were validated by comparing the experimental studies reported in the literature. It was found that all surface tension models can describe the bubble rise and bubble plume in a column with slight deviations. 展开更多
关键词 BUBBLE column reactor Computational FLUID dynamics Volume of FLUID method Surface tension MODELS Gas DISTRIBUTOR
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Visual experimental study of nanofluids application to promote CO_(2) absorption in a bubble column
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作者 Shangyuan Cheng Guisheng Qi +1 位作者 Yuliang Li Yixuan Yang 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第3期228-237,共10页
The addition of dispersed-phase nanoparticles in the liquid phase can enhance the gas-liquid transfer process as the suspended nanoparticles affect the transfer process inside the fluid through microdisturbance or mic... The addition of dispersed-phase nanoparticles in the liquid phase can enhance the gas-liquid transfer process as the suspended nanoparticles affect the transfer process inside the fluid through microdisturbance or micro-convection effects.In this article,a high-speed digital camera was used to visualize the bubble behavior of CO_(2) in pure water and nanofluids to examine the effects of CO_(2) gas flow rate,nanoparticle solid content and type on the bubble behavior in the fluids.The CO_(2) absorption performance in three water-based nanofluids were compared in a bubbler.And the mass transfer characteristics during CO_(2) bubble absorption and the reasons for the enhanced gas-liquid mass transfer effect of nanoparticles were analyzed.The results showed that the presence of nanoparticles affected the formation process of bubbles in the fluid,shortened the bubble detachment time,reduced the detachment diameter,effectively increased the gas-liquid contact area,and improved the bubbles detachment frequency.The system with MCM-41 corresponded to a higher overall mass transfer coefficient.Uncalined MCM-41 contained surfactant that enhanced foaming behavior in water.This prevented the transfer of CO_(2) to some extent,and the CO_(2) absorption by uncalined MCM-41/H_(2)O was 5.34%higher than that by pure water.Compared with SiO_(2) nanoparticles with the same particle size and the same composition,MCM-41 had a higher adsorption capacity and better hydrophilicity due to its larger specific surface area and rich porous structure,which was more favorable to accelerate the collision between nanoparticles and CO_(2) bubbles to cause micro-convection.Under the condition of 0.1%(mass)solid content,the enhancement of CO_(2) absorption process by MCM-41 nanoparticles was more significant and improved by 16.9%compared with pure water. 展开更多
关键词 Nanofluids CO_(2) absorption Mass transfer MCM-41 Bubble column
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Seismic Behavior of Diaphragm-Through Connections of Concrete-Filled Square Steel Tubular Columns and H-Shaped Steel Beams 被引量:6
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作者 荣彬 陈志华 +1 位作者 Apostolos Fafitis 苗纪奎 《Transactions of Tianjin University》 EI CAS 2013年第3期195-201,共7页
Based on the introductions of a type of diaphragm-through connection between concrete-filled square steel tubular columns (CFSSTCs) and H-shaped steel beams,a finite element model of the connection is developed and us... Based on the introductions of a type of diaphragm-through connection between concrete-filled square steel tubular columns (CFSSTCs) and H-shaped steel beams,a finite element model of the connection is developed and used to investigate the seismic behavior of the connection.The results of the finite element model are validated by a set of cyclic loading tests.The cyclic loading tests and the finite element analyses indicate that the failure mode of the suggested connections is plastic hinge at the beam with inelastic rotation angle exceeding 0.04 rad.The suggested connections have sufficient strength,plastic deformation and energy dissipation capacity to be used in composite moment frames as beam-to-column rigid connections. 展开更多
关键词 concrete-filled square STEEL TUBULAR column H-shaped STEEL beam diaphragm-through connection seismic behavior load transfer mechanism
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