In this study,Paprika 247 with milk white fruit at marketable maturity stage as male parent and capsicum 246 with green fruit at marketable maturity stage as female parent were selected as experimental materials to sc...In this study,Paprika 247 with milk white fruit at marketable maturity stage as male parent and capsicum 246 with green fruit at marketable maturity stage as female parent were selected as experimental materials to screen SSR molecular markers closely related to white fruit character,so as to lay a foundation for the use of SSR technique for molecular marker-assisted breeding and provide reference for breeding of new white capsicum germplasms and varieties. 360 pairs of SSR primers were screened in total,and among them,3 pairs of primers（ ES-89,ES-292,ES-296） exhibited remarkable polymorphic differences. This study will provide a basis for next QTL mapping.展开更多
The bsd-pg（bundle sheath defective pale green） mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maiz...The bsd-pg（bundle sheath defective pale green） mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing（SLAF-Seq） technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2：1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats（SSRs） markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.展开更多
The molecular variation and genetic relationships among five populations of the fall webworm （Hyphantria cunea Drury） in China were assessed using microsatellite technology. Ten microsatellite primers, producing pol...The molecular variation and genetic relationships among five populations of the fall webworm （Hyphantria cunea Drury） in China were assessed using microsatellite technology. Ten microsatellite primers, producing polymorphic bands, were used across 300 samples. The percentage of polymorphic loci （PPB） was 98.36%; the percentage of polymorphic loci in five populations ranged from high to low in the following order： Cangzhou population, Yantai population, Qinhuangdao population, Dandong population, and Shijiazhuang population. The results showed that 34.38% of the total genetic variation of the fall webworm （GsT） occurs among populations, while most variation （65.62%） exists within populations. Nei＇s genetic distances ranged from 0.1386 to 0.3224. Using the unweighted pair group method with arithmetic mean （UPGMA）, Nei＇s genetic distances were analyzed by a clustering technique and the dendrogram shows that population differentiation is closely related to the time and geographic origin of the invasion. The major factors impacting genetic diversity of fall webworm populations are longitude, the plain area ratio, annual precipitation, latitude and time of invasion. The formation of genetic structure is correlated with characteristics of the life history and invasion ecology of the species.展开更多
基金Supported by College Students'Innovation and Enterpreneurship Training Program of China in 2017(201710364078)Anhui Science and Technology Major Project(15czz03120)
文摘In this study,Paprika 247 with milk white fruit at marketable maturity stage as male parent and capsicum 246 with green fruit at marketable maturity stage as female parent were selected as experimental materials to screen SSR molecular markers closely related to white fruit character,so as to lay a foundation for the use of SSR technique for molecular marker-assisted breeding and provide reference for breeding of new white capsicum germplasms and varieties. 360 pairs of SSR primers were screened in total,and among them,3 pairs of primers（ ES-89,ES-292,ES-296） exhibited remarkable polymorphic differences. This study will provide a basis for next QTL mapping.
基金supported by the National Natural Science Foundation of China (30700476 and 31071057)the Beijing Natural Science Foundation, China (5083021)
文摘The bsd-pg（bundle sheath defective pale green） mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing（SLAF-Seq） technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2：1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats（SSRs） markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.
基金supported by the National Natural Science Foundation of China (Grant No. 30771739)
文摘The molecular variation and genetic relationships among five populations of the fall webworm （Hyphantria cunea Drury） in China were assessed using microsatellite technology. Ten microsatellite primers, producing polymorphic bands, were used across 300 samples. The percentage of polymorphic loci （PPB） was 98.36%; the percentage of polymorphic loci in five populations ranged from high to low in the following order： Cangzhou population, Yantai population, Qinhuangdao population, Dandong population, and Shijiazhuang population. The results showed that 34.38% of the total genetic variation of the fall webworm （GsT） occurs among populations, while most variation （65.62%） exists within populations. Nei＇s genetic distances ranged from 0.1386 to 0.3224. Using the unweighted pair group method with arithmetic mean （UPGMA）, Nei＇s genetic distances were analyzed by a clustering technique and the dendrogram shows that population differentiation is closely related to the time and geographic origin of the invasion. The major factors impacting genetic diversity of fall webworm populations are longitude, the plain area ratio, annual precipitation, latitude and time of invasion. The formation of genetic structure is correlated with characteristics of the life history and invasion ecology of the species.
