Objective To discuss the genetic relationship between Chaoxian and the other populations with STR polymorphisms, and study the origination of Chaoxian with historical data, analyze the difference among them. Methods S...Objective To discuss the genetic relationship between Chaoxian and the other populations with STR polymorphisms, and study the origination of Chaoxian with historical data, analyze the difference among them. Methods Samples were obtained from 91 unrelated individuals of Jilin province. PCR amplification was performed using PE9700, the results were analyzed with the GeneScan software and then using the Genetic Analyzer ABI377 and Genotyper2.5. Tests for Hardy Weinberg equilibrium (HWE) and the clustering map were carried out using SPSS11.5. The DA (genetic distance) according to Nei's was calculated and a phylogenetic tree based on the neighbor-joining method using Mega2 software package was constructed. Results 81 alleles and 196 genotypes were observed, with the corresponding frequencies ranging from 0.0055 to 0.4615 and 0.0110 to 0.2747, the observed and expected of genotypes were evaluated using χ 2-test and all of the loci were in accordance with Hardy Weinberg equilibrium (P> 0.05). Chaoxian was clustered with the other Chinese ethnic groups, then the American Black and the White; it was clustered with the Baishan Han, the Beijing Han of China first, then the South Korean. Conclusion The differentiation among races is larger than that among minorities, and, the differentiation among minorities is related to and is consistent with their geographic location.展开更多
Objective To study the STR polymorphism in Chinese Drungs. Methods The genetic distributions of 15 STR loci and Amelogenin locus were generated through coamplification, genescan and genotype from 65 Drungs. Results...Objective To study the STR polymorphism in Chinese Drungs. Methods The genetic distributions of 15 STR loci and Amelogenin locus were generated through coamplification, genescan and genotype from 65 Drungs. Results There were 144 STR and 2 Amelogenin alleles in Drung nationality, with their frequencies ranging from 0.0077 to 0.7846, H 0.3723～0.8639, DP 0.5567～0.9548, EPP 0.2738～0.8358, and PIC 0.3461～0.8456, the accumulative DP 0.99999998 and EPP 0.99999894. Conclusion The study founded a basis for the genetic structure of Chinese ethnic groups,which is useful for the application to anthropology and forensic science.展开更多
During World War Ⅱ,many nations took part in the war.Among the supporters of the Alliance there was also Brazil.In August 1944,under the leadership of President Getúlio Vargas,Brazil declared war on Nazi Germany...During World War Ⅱ,many nations took part in the war.Among the supporters of the Alliance there was also Brazil.In August 1944,under the leadership of President Getúlio Vargas,Brazil declared war on Nazi Germany and took part in the Italian campaign by send-ing many troops to support the Allies in the Central Italy.Once the conflict was over,the deceased Brazilian soldiers were buried in Pistoia,a few kilometers from Florence.But only in 1960 the Brazilian government authorized the transfer of the dead soldiers to their home-land.Five years later,during the building of the Brazilian Military Votive Monument,still in the Pistoia cemetery,a last body was found but could not be identified:so he was buried as an"unknown soldier".In December 2012,the Brazilian Embassy in Italy asked for performing forensic genetics analysis for identification purposes on the remains of this last unknown Brazilian soldier.After almost 70years a complete short tandem repeats(STR)profile was obtained,useful for any relatives searching.展开更多
文摘Objective To discuss the genetic relationship between Chaoxian and the other populations with STR polymorphisms, and study the origination of Chaoxian with historical data, analyze the difference among them. Methods Samples were obtained from 91 unrelated individuals of Jilin province. PCR amplification was performed using PE9700, the results were analyzed with the GeneScan software and then using the Genetic Analyzer ABI377 and Genotyper2.5. Tests for Hardy Weinberg equilibrium (HWE) and the clustering map were carried out using SPSS11.5. The DA (genetic distance) according to Nei's was calculated and a phylogenetic tree based on the neighbor-joining method using Mega2 software package was constructed. Results 81 alleles and 196 genotypes were observed, with the corresponding frequencies ranging from 0.0055 to 0.4615 and 0.0110 to 0.2747, the observed and expected of genotypes were evaluated using χ 2-test and all of the loci were in accordance with Hardy Weinberg equilibrium (P> 0.05). Chaoxian was clustered with the other Chinese ethnic groups, then the American Black and the White; it was clustered with the Baishan Han, the Beijing Han of China first, then the South Korean. Conclusion The differentiation among races is larger than that among minorities, and, the differentiation among minorities is related to and is consistent with their geographic location.
基金ThesubjectwassupportedbyNationalNatureScienceFoundationofChina (No .39970 40 1)
文摘Objective To study the STR polymorphism in Chinese Drungs. Methods The genetic distributions of 15 STR loci and Amelogenin locus were generated through coamplification, genescan and genotype from 65 Drungs. Results There were 144 STR and 2 Amelogenin alleles in Drung nationality, with their frequencies ranging from 0.0077 to 0.7846, H 0.3723～0.8639, DP 0.5567～0.9548, EPP 0.2738～0.8358, and PIC 0.3461～0.8456, the accumulative DP 0.99999998 and EPP 0.99999894. Conclusion The study founded a basis for the genetic structure of Chinese ethnic groups,which is useful for the application to anthropology and forensic science.
文摘During World War Ⅱ,many nations took part in the war.Among the supporters of the Alliance there was also Brazil.In August 1944,under the leadership of President Getúlio Vargas,Brazil declared war on Nazi Germany and took part in the Italian campaign by send-ing many troops to support the Allies in the Central Italy.Once the conflict was over,the deceased Brazilian soldiers were buried in Pistoia,a few kilometers from Florence.But only in 1960 the Brazilian government authorized the transfer of the dead soldiers to their home-land.Five years later,during the building of the Brazilian Military Votive Monument,still in the Pistoia cemetery,a last body was found but could not be identified:so he was buried as an"unknown soldier".In December 2012,the Brazilian Embassy in Italy asked for performing forensic genetics analysis for identification purposes on the remains of this last unknown Brazilian soldier.After almost 70years a complete short tandem repeats(STR)profile was obtained,useful for any relatives searching.
