目的对湖南汉族人群20个常染色体短串联重复序列(short tandem repeat,STR)基因座的等位基因分布、群体遗传学参数和邻近群体的遗传分析进行研究,评估其在法医学中的应用价值。方法应用Power-Plex21^(■) 试剂盒对2997例湖南汉族无关个...目的对湖南汉族人群20个常染色体短串联重复序列(short tandem repeat,STR)基因座的等位基因分布、群体遗传学参数和邻近群体的遗传分析进行研究,评估其在法医学中的应用价值。方法应用Power-Plex21^(■) 试剂盒对2997例湖南汉族无关个体进行20个STR基因座复合扩增及等位基因分型,统计等位基因频率及群体遗传学参数,计算湖南汉族与已公开报道的13个群体间的Nei’s遗传距离,进行多维尺度分析并构建系统发生树。结果20个常染色体STR基因座的杂合度为0.6009~0.9116,个人识别能力为0.7745~0.9866,三联体非父排除率为0.2920~0.8191,二联体非父排除率为0.1910~0.7086,多态信息含量为0.5348~0.9093,基因型分布符合Hardy-Weinberg平衡。20个基因座的累积个体识别率为1-1.3509×10^(-21),三联体非父累积排除率与二联体非父累积排除率分别为0.999996523945999、0.999999996129773,基于湖南汉族人群与其他13个群体间遗传距离获得的多维尺度分析及系统发生树结果显示,其与湖北汉族相距较近,而与云南苗族最远。结论20个常染色体STR基因座在湖南汉族人群中呈高度多态性和良好的鉴别能力,能为该地区法医学个体识别、亲权鉴定和群体学研究提供基础数据。展开更多
Objective To investigate the genetic relation am on g ten ethnic groups in northwest China. Methods Allele frequ encies of six STR(short tandem repeat ) loci in D13S1358, VWA, FGA, D5S818, D13S 317, and D7S820 were ...Objective To investigate the genetic relation am on g ten ethnic groups in northwest China. Methods Allele frequ encies of six STR(short tandem repeat ) loci in D13S1358, VWA, FGA, D5S818, D13S 317, and D7S820 were collected from Lasa Tibetan, Changdu Tibetan, Xi'an Han, G ansu Dongxiang, Gansu Yugu, Xinjiang Uygur, Ozbak, Kirgiz, Sibe, Ningxia Hui by the results of State Key laboratory, Forensic Science Department, school of medi cal, Xi'an Jiaotong University and internet biological information data bank, a nd compared with that of the Mongolian, Zhuang in China, and White and Negro in USA. The polymorphism index (H, DP, PPE, PIC) and genetic distance, then the phy logenetic tree of all population were reported. Results The res ulting tree topology exhibited strong geographic and racial partitioning consist ent with that obtained with HLA and classical genetic polymorphisms. Conclusion The results suggest that forensic STR loci may be particular ly powerful tools and provide the necessary fine resolution for the reconstructi on of recent human evolutionary history.展开更多
文摘目的对湖南汉族人群20个常染色体短串联重复序列(short tandem repeat,STR)基因座的等位基因分布、群体遗传学参数和邻近群体的遗传分析进行研究,评估其在法医学中的应用价值。方法应用Power-Plex21^(■) 试剂盒对2997例湖南汉族无关个体进行20个STR基因座复合扩增及等位基因分型,统计等位基因频率及群体遗传学参数,计算湖南汉族与已公开报道的13个群体间的Nei’s遗传距离,进行多维尺度分析并构建系统发生树。结果20个常染色体STR基因座的杂合度为0.6009~0.9116,个人识别能力为0.7745~0.9866,三联体非父排除率为0.2920~0.8191,二联体非父排除率为0.1910~0.7086,多态信息含量为0.5348~0.9093,基因型分布符合Hardy-Weinberg平衡。20个基因座的累积个体识别率为1-1.3509×10^(-21),三联体非父累积排除率与二联体非父累积排除率分别为0.999996523945999、0.999999996129773,基于湖南汉族人群与其他13个群体间遗传距离获得的多维尺度分析及系统发生树结果显示,其与湖北汉族相距较近,而与云南苗族最远。结论20个常染色体STR基因座在湖南汉族人群中呈高度多态性和良好的鉴别能力,能为该地区法医学个体识别、亲权鉴定和群体学研究提供基础数据。
文摘Objective To investigate the genetic relation am on g ten ethnic groups in northwest China. Methods Allele frequ encies of six STR(short tandem repeat ) loci in D13S1358, VWA, FGA, D5S818, D13S 317, and D7S820 were collected from Lasa Tibetan, Changdu Tibetan, Xi'an Han, G ansu Dongxiang, Gansu Yugu, Xinjiang Uygur, Ozbak, Kirgiz, Sibe, Ningxia Hui by the results of State Key laboratory, Forensic Science Department, school of medi cal, Xi'an Jiaotong University and internet biological information data bank, a nd compared with that of the Mongolian, Zhuang in China, and White and Negro in USA. The polymorphism index (H, DP, PPE, PIC) and genetic distance, then the phy logenetic tree of all population were reported. Results The res ulting tree topology exhibited strong geographic and racial partitioning consist ent with that obtained with HLA and classical genetic polymorphisms. Conclusion The results suggest that forensic STR loci may be particular ly powerful tools and provide the necessary fine resolution for the reconstructi on of recent human evolutionary history.
