Topotecan (TPT), a semisynthetic analogue of the natural product camptothecin is a cell cycle-specific drug with antitumor activity. To clarify the effect of TPT on SUD4 and DOHH2 cell line in this study, we examined ...Topotecan (TPT), a semisynthetic analogue of the natural product camptothecin is a cell cycle-specific drug with antitumor activity. To clarify the effect of TPT on SUD4 and DOHH2 cell line in this study, we examined the apoptosis and cell cycle changes of the two human cancer cell lines by exposing to TPT for 18 hours at various concentrations. The linear relationship between apoptosis cell number and the concentration of TPT was observed by means of Flow Cytometry and Annexin V assay. Then, DOHH2 cell is much more sensitive to TPT than SUD4 cell. In addition, Cell Question Software Assay showed positive relationship between the frequency of cells accumulated in S-phase and the concentration of TPT. The least concentration of TPT to change cell cycle is 5 nmol·L?1 in both cell lines. These results suggest that the inducing apoptosis of cancer cells is one of mechanism of TPT antitumor activity.展开更多
Se trouvant dans le sud-est de la Chine au bord dela mer, la province du Fujian s’appelle aussi toutsimplement "Min". Les anc(?)tres d’une grande partie de lapopulation de Taiwan sont venus du Fujian (Min)...Se trouvant dans le sud-est de la Chine au bord dela mer, la province du Fujian s’appelle aussi toutsimplement "Min". Les anc(?)tres d’une grande partie de lapopulation de Taiwan sont venus du Fujian (Min). Les展开更多
John Howe先生是英国土木工程师学会和澳大利亚土木工程师学会的注册工程师．在澳大利亚凯恩斯机场、香港新机场和深圳盐田港工程建设时期．曾担任建设方在混凝土路面砖路面工程的技术总监；并亲手创建英国混凝土路面砖协会，参与英国...John Howe先生是英国土木工程师学会和澳大利亚土木工程师学会的注册工程师．在澳大利亚凯恩斯机场、香港新机场和深圳盐田港工程建设时期．曾担任建设方在混凝土路面砖路面工程的技术总监；并亲手创建英国混凝土路面砖协会，参与英国混凝土路面砖产品领域相关产品标；住、设计施工规程的制定.John Howe先生还是“国际小型混凝土预制构件委员会（SEPT）”成员、2012年11月在上海召开“第十届国际混凝土路面砖大会”期间．中国建筑砌块协会曾特别邀请他．组织举办了一场“英国可持续排水（SuDs）与透水路面”的公开讲座一考虑到目前我国“海绵城市建设”工程缺乏必要的技术支撑．本刊编辑部特将IohnHowe先生当时的演讲内容，编辑整理加工成“英国可持续排水（Suds）与透水路面的关系”和“英国缝隙透水混凝土路面砖路面设计与施工方法概要”两篇文章进行连载（本期和下期）．供广大读者借鉴、季成云先生和沈玉露女士为编辑整理这两篇文章．提供了翻译、记录的素材、展开更多
Objective: We studied the feasibility of using Alcohol, Smoking, and Substance Involvement Screening Test (ASSIST) linked with Brief Intervention (ALBI) in treating Substance Use Disorder in Nigeria. There is a clear ...Objective: We studied the feasibility of using Alcohol, Smoking, and Substance Involvement Screening Test (ASSIST) linked with Brief Intervention (ALBI) in treating Substance Use Disorder in Nigeria. There is a clear gap in meeting the needs for treatment and care for people with drug use disorders in Nigeria with many users reporting a self-perceived need for treatment and about 40% of them wanting to receive drug treatment but were unable to access such services. Methods: ASSIST questionnaire was used to gather baseline data, and ALBI along with Motivational Interviewing (MI) was used to intervene for 6 weeks following which ASSIST score was repeated and compared with baseline. Results: Majority of the participants were aged 21 - 30 years, mostly males with (61.3%) lacking steady jobs. Lifetime prevalence of any drug use was (88.2%). Three months prevalence was nicotine (72.0%), alcohol (66.7%), cannabis (47.3%), opioids (11.8%) among others. The ASSIST scores were mostly on the severe ranges. ASSIST result after six weeks showed marked reduction. The Mean Difference (MD) in the ASSIST scores after intervention was significant for tobacco, opioids, alcohol, and cannabis. The Cohen’s D effect size was large for tobacco (1.08), alcohol (0.92) and cannabis (0.73) but low for other substances. Conclusion: ALBI along with MI was helpful in combating the menace of substance use.展开更多
Geothermal heat pumps （GHPs） are an attractive proposition for renewable energy worldwide as it uses energy naturally stored in the earth. The Earth is a very resourceful form of energy, using the natural solar ener...Geothermal heat pumps （GHPs） are an attractive proposition for renewable energy worldwide as it uses energy naturally stored in the earth. The Earth is a very resourceful form of energy, using the natural solar energy collection and heat storage capabilities as an infinite heat source/heat sink at the base of permeable pavements, which can provide excellent temperature gradients for GHP＇s. Experimental rigs were setup up at The University of Edinbttrgh for a combined permeable pavement and GHP system. At the base of a pavement structure （approximately 1 meter） below the ground＇s surface, temperatures are constant of 10℃ in the U.K all year round. The GHP performance efficiency was analysed by the coefficient of performance （COP） in a heating cycle and the energy efficiency ratio （EER） in a cooling cycle. The mean COP and EER for both systems averaged between 2-4.5 and 3-5 respectively. The combined GHP and pavement structure operated at an optimum efficiency for both heating and cooling cycles and has shown to be unaffected by higher summer or lower winter temperatures. This hybrid system is an attractive renewable energy technology and has additional environmental benefits such as urban runoff reuse and recycling.展开更多
文摘Topotecan (TPT), a semisynthetic analogue of the natural product camptothecin is a cell cycle-specific drug with antitumor activity. To clarify the effect of TPT on SUD4 and DOHH2 cell line in this study, we examined the apoptosis and cell cycle changes of the two human cancer cell lines by exposing to TPT for 18 hours at various concentrations. The linear relationship between apoptosis cell number and the concentration of TPT was observed by means of Flow Cytometry and Annexin V assay. Then, DOHH2 cell is much more sensitive to TPT than SUD4 cell. In addition, Cell Question Software Assay showed positive relationship between the frequency of cells accumulated in S-phase and the concentration of TPT. The least concentration of TPT to change cell cycle is 5 nmol·L?1 in both cell lines. These results suggest that the inducing apoptosis of cancer cells is one of mechanism of TPT antitumor activity.
文摘Se trouvant dans le sud-est de la Chine au bord dela mer, la province du Fujian s’appelle aussi toutsimplement "Min". Les anc(?)tres d’une grande partie de lapopulation de Taiwan sont venus du Fujian (Min). Les
文摘Objective: We studied the feasibility of using Alcohol, Smoking, and Substance Involvement Screening Test (ASSIST) linked with Brief Intervention (ALBI) in treating Substance Use Disorder in Nigeria. There is a clear gap in meeting the needs for treatment and care for people with drug use disorders in Nigeria with many users reporting a self-perceived need for treatment and about 40% of them wanting to receive drug treatment but were unable to access such services. Methods: ASSIST questionnaire was used to gather baseline data, and ALBI along with Motivational Interviewing (MI) was used to intervene for 6 weeks following which ASSIST score was repeated and compared with baseline. Results: Majority of the participants were aged 21 - 30 years, mostly males with (61.3%) lacking steady jobs. Lifetime prevalence of any drug use was (88.2%). Three months prevalence was nicotine (72.0%), alcohol (66.7%), cannabis (47.3%), opioids (11.8%) among others. The ASSIST scores were mostly on the severe ranges. ASSIST result after six weeks showed marked reduction. The Mean Difference (MD) in the ASSIST scores after intervention was significant for tobacco, opioids, alcohol, and cannabis. The Cohen’s D effect size was large for tobacco (1.08), alcohol (0.92) and cannabis (0.73) but low for other substances. Conclusion: ALBI along with MI was helpful in combating the menace of substance use.
文摘Geothermal heat pumps （GHPs） are an attractive proposition for renewable energy worldwide as it uses energy naturally stored in the earth. The Earth is a very resourceful form of energy, using the natural solar energy collection and heat storage capabilities as an infinite heat source/heat sink at the base of permeable pavements, which can provide excellent temperature gradients for GHP＇s. Experimental rigs were setup up at The University of Edinbttrgh for a combined permeable pavement and GHP system. At the base of a pavement structure （approximately 1 meter） below the ground＇s surface, temperatures are constant of 10℃ in the U.K all year round. The GHP performance efficiency was analysed by the coefficient of performance （COP） in a heating cycle and the energy efficiency ratio （EER） in a cooling cycle. The mean COP and EER for both systems averaged between 2-4.5 and 3-5 respectively. The combined GHP and pavement structure operated at an optimum efficiency for both heating and cooling cycles and has shown to be unaffected by higher summer or lower winter temperatures. This hybrid system is an attractive renewable energy technology and has additional environmental benefits such as urban runoff reuse and recycling.
