SWEET(sugars will eventually be exported transporters)基因广泛存在于植物、动物和微生物中,在细胞膜或者细胞器膜形成跨膜孔道,协助糖类物质完成跨膜运输。拟南芥SWEET1/2/3基因属于SWEET基因家族CladeⅠ分支。通过表达谱数据分析...SWEET(sugars will eventually be exported transporters)基因广泛存在于植物、动物和微生物中,在细胞膜或者细胞器膜形成跨膜孔道,协助糖类物质完成跨膜运输。拟南芥SWEET1/2/3基因属于SWEET基因家族CladeⅠ分支。通过表达谱数据分析发现,SWEET1基因在花器官优先表达,SWEET2基因在营养生长和生殖生长期均有表达,SWEET3基因在花中表达。三个基因在体外鉴定具有葡萄糖转运活性,但由于缺少功能缺失的突变体和功能冗余,它们的生理功能仍不清楚。通过CRISPR/Cas9系统在SWEET1/2/3基因中创建了靶向突变,鉴定获得了sweet1、sweet1/2、sweet3和sweet1/2/3突变体。突变体在营养生长过程中表型与野生型相同,但它们的角果长度显著短于野生型的角果。在高浓度葡萄糖培养基上,sweet1、sweet1/2和sweet1/2/3突变体对葡萄糖敏感,其特征是根更短,高度严重降低,表明SWEET1/2/3基因在葡萄糖信号中具有功能。对葡萄糖信号通路中关键基因的进一步分析发现,HXK1、KIN10和KIN11在野生型和突变体之间转录和翻译水平没有显著差异。结果表明,拟南芥SWEET1/2/3基因在葡萄糖信号传导和调节角果的发育中起着重要作用。展开更多
Sugars,especially glucose and fructose,contribute to the taste and quality of tomato fruits.These compounds are translocated from the leaves to the fruits and then unloaded into the fruits by various sugar transporter...Sugars,especially glucose and fructose,contribute to the taste and quality of tomato fruits.These compounds are translocated from the leaves to the fruits and then unloaded into the fruits by various sugar transporters at the plasma membrane.SWEETs,are sugar transporters that regulate sugar efflux independently of energy or pH.To date,the role of SWEETs in tomato has received very little attention.In this study,we performed functional analysis of SlSWEET7a and SlSWEET14 to gain insight into the regulation of sugar transport and storage in tomato fruits.SlSWEET7a and SlSWEET14 were mainly expressed in peduncles,vascular bundles,and seeds.Both SlSWEET7a and SlSWEET14 are plasma membrane-localized proteins that transport fructose,glucose,and sucrose.Apart from the resulting increase in mature fruit sugar content,silencing SlSWEET7a or SlSWEET14 resulted in taller plants and larger fruits(in SlSWEET7a-silenced lines).We also found that invertase activity and gene expression of some SlSWEET members increased,which was consistent with the increased availability of sucrose and hexose in the fruits.Overall,our results demonstrate that suppressing SlSWEET7a and SlSWEET14 could be a potential strategy for enhancing the sugar content of tomato fruits.展开更多
SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in plants.While the functions of SWEETs have been well established in model plants...SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in plants.While the functions of SWEETs have been well established in model plants such as Arabidopsis,their functions in economically important fruit crops like pineapple have not been well studied.Here we aimed to investigate the substrate specificity of pineapple SWEETs by comparing the protein sequences of known glucose and sucrose transporters in Arabidopsis with those in pineapple.Our genome-wide approach and 3D structure comparison showed that the Arabidopsis SWEET8 homolog in pineapple,AcSWEET10,shares similar sequences and protein properties responsible for glucose transport.To determine the functional conservation of AcSWEET10,we tested its ability to complement glucose transport mutants in yeast and analyzed its expression in stamens and impact on the microspore phenotype and seed set in transgenic Arabidopsis.The results showed that AcSWEET10 is functionally equivalent to AtSWEET8 and plays a critical role in regulating microspore formation through the regulation of the Callose synthase5(CalS5),which highlights the importance of SWEET transporters in pineapple.This information could have important implications for improving fruit crop yield and quality by manipulating SWEET transporter activity.展开更多
Seed weight and size are critical agronomic traits essential for determining yield and composition,receiving significant attention throughout domestication(Meyer et al.,2012).Seed weight serves as a reliable indicator...Seed weight and size are critical agronomic traits essential for determining yield and composition,receiving significant attention throughout domestication(Meyer et al.,2012).Seed weight serves as a reliable indicator of average seed size due to their positive correlation.Regulation of seed weight involves both developmental and environmental cues,affecting cell proliferation or expansion(Li et al.,2019).展开更多
Background: Imatinib mesylate has become one of the main chemotherapeutic agents currently used to treat patients with chronic myeloid leukemia (CML). Although cutaneous reactions to this drug have been documented bef...Background: Imatinib mesylate has become one of the main chemotherapeutic agents currently used to treat patients with chronic myeloid leukemia (CML). Although cutaneous reactions to this drug have been documented before, this is the first time that Sweet syndrome has been reported with its use. Observations: We report a case of Sweet syndrome secondary to the administration of imatinib to treat CML. On 2 separate occasions, a 53-year-old African American woman with CML developed neutrophilic dermatosis consistent with Sweet syndrome after chemotherapy with imatinib. Conclusion: Greater awareness of the adverse effects of imatinib and the character- ization of its cutaneous adverse effects will lead to improved surveillance for and treatment of those adverse effects.展开更多
David Mungania has always dreamt of owning a house. But the major obstacle for him is the high cost of housing. Being a tenant is costly and the stress of finding money for the high monthly rent adds to his woes.
文摘SWEET(sugars will eventually be exported transporters)基因广泛存在于植物、动物和微生物中,在细胞膜或者细胞器膜形成跨膜孔道,协助糖类物质完成跨膜运输。拟南芥SWEET1/2/3基因属于SWEET基因家族CladeⅠ分支。通过表达谱数据分析发现,SWEET1基因在花器官优先表达,SWEET2基因在营养生长和生殖生长期均有表达,SWEET3基因在花中表达。三个基因在体外鉴定具有葡萄糖转运活性,但由于缺少功能缺失的突变体和功能冗余,它们的生理功能仍不清楚。通过CRISPR/Cas9系统在SWEET1/2/3基因中创建了靶向突变,鉴定获得了sweet1、sweet1/2、sweet3和sweet1/2/3突变体。突变体在营养生长过程中表型与野生型相同,但它们的角果长度显著短于野生型的角果。在高浓度葡萄糖培养基上,sweet1、sweet1/2和sweet1/2/3突变体对葡萄糖敏感,其特征是根更短,高度严重降低,表明SWEET1/2/3基因在葡萄糖信号中具有功能。对葡萄糖信号通路中关键基因的进一步分析发现,HXK1、KIN10和KIN11在野生型和突变体之间转录和翻译水平没有显著差异。结果表明,拟南芥SWEET1/2/3基因在葡萄糖信号传导和调节角果的发育中起着重要作用。
基金This research was supported by the National Key Research and Development Program of China(2019YFD100030)the National Natural Science Foundation of China(No.31372054).
文摘Sugars,especially glucose and fructose,contribute to the taste and quality of tomato fruits.These compounds are translocated from the leaves to the fruits and then unloaded into the fruits by various sugar transporters at the plasma membrane.SWEETs,are sugar transporters that regulate sugar efflux independently of energy or pH.To date,the role of SWEETs in tomato has received very little attention.In this study,we performed functional analysis of SlSWEET7a and SlSWEET14 to gain insight into the regulation of sugar transport and storage in tomato fruits.SlSWEET7a and SlSWEET14 were mainly expressed in peduncles,vascular bundles,and seeds.Both SlSWEET7a and SlSWEET14 are plasma membrane-localized proteins that transport fructose,glucose,and sucrose.Apart from the resulting increase in mature fruit sugar content,silencing SlSWEET7a or SlSWEET14 resulted in taller plants and larger fruits(in SlSWEET7a-silenced lines).We also found that invertase activity and gene expression of some SlSWEET members increased,which was consistent with the increased availability of sucrose and hexose in the fruits.Overall,our results demonstrate that suppressing SlSWEET7a and SlSWEET14 could be a potential strategy for enhancing the sugar content of tomato fruits.
基金We especially thank Dr Binghua Wu(Fujian Agriculture and Forestry University,China)for kindly providing the yeast mutant strain EBY.VW4000 and Prof.Zhong-Nan Yang(Shanghai Normal University,China)for sharing Atsweet8 seeds.This work was supported by the Science and Technology Major Project of Guangxi(Gui Ke AA22068096)the Science and Technology Innovation Project of Pingtan Science and Technology Research Institute(PT2021007,PT2021003)+2 种基金the General Project of Fujian Natural Science Foundation(2020 J01594)the Project of Guangxi Featured Fruit Innovation Team on Pineapple Breeding and Cultivation Post under the National Modern Agricultural Industry Technology System(nycytxgxcxtd-17-05)the Guangxi Academy of Agricultural Sciences Basic Research Project(Gui Nong Ke 2021YT046).The funding bodies played no role in the design of the study and collection,analysis and interpretation of the data,and writing the manuscript.
文摘SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in plants.While the functions of SWEETs have been well established in model plants such as Arabidopsis,their functions in economically important fruit crops like pineapple have not been well studied.Here we aimed to investigate the substrate specificity of pineapple SWEETs by comparing the protein sequences of known glucose and sucrose transporters in Arabidopsis with those in pineapple.Our genome-wide approach and 3D structure comparison showed that the Arabidopsis SWEET8 homolog in pineapple,AcSWEET10,shares similar sequences and protein properties responsible for glucose transport.To determine the functional conservation of AcSWEET10,we tested its ability to complement glucose transport mutants in yeast and analyzed its expression in stamens and impact on the microspore phenotype and seed set in transgenic Arabidopsis.The results showed that AcSWEET10 is functionally equivalent to AtSWEET8 and plays a critical role in regulating microspore formation through the regulation of the Callose synthase5(CalS5),which highlights the importance of SWEET transporters in pineapple.This information could have important implications for improving fruit crop yield and quality by manipulating SWEET transporter activity.
基金supported by the I.C.Gunsalus Scholar and a seed-funding from the Center for Digital Agriculture to L.-Q.C.from the University of Illinois Urbana-Champaign,UnitedStates.
文摘Seed weight and size are critical agronomic traits essential for determining yield and composition,receiving significant attention throughout domestication(Meyer et al.,2012).Seed weight serves as a reliable indicator of average seed size due to their positive correlation.Regulation of seed weight involves both developmental and environmental cues,affecting cell proliferation or expansion(Li et al.,2019).
文摘Background: Imatinib mesylate has become one of the main chemotherapeutic agents currently used to treat patients with chronic myeloid leukemia (CML). Although cutaneous reactions to this drug have been documented before, this is the first time that Sweet syndrome has been reported with its use. Observations: We report a case of Sweet syndrome secondary to the administration of imatinib to treat CML. On 2 separate occasions, a 53-year-old African American woman with CML developed neutrophilic dermatosis consistent with Sweet syndrome after chemotherapy with imatinib. Conclusion: Greater awareness of the adverse effects of imatinib and the character- ization of its cutaneous adverse effects will lead to improved surveillance for and treatment of those adverse effects.
文摘David Mungania has always dreamt of owning a house. But the major obstacle for him is the high cost of housing. Being a tenant is costly and the stress of finding money for the high monthly rent adds to his woes.
