Candida shehatae gene xyl1 and Pichia stipitis gene xyl2,encoding xylose reductase(XR)and xylitol dehydrogenase(XD)respectively,were amplified by PCR.The genes xyl1 and xyl2 were placed under the control of promoter G...Candida shehatae gene xyl1 and Pichia stipitis gene xyl2,encoding xylose reductase(XR)and xylitol dehydrogenase(XD)respectively,were amplified by PCR.The genes xyl1 and xyl2 were placed under the control of promoter GAL in vector pYES2 to construct the recombinant expression vector pYES2-P12.Subsequently the vector pYES2-P12 was transformed into S.cerevisiae YS58 by LiAc to produce the recombinant yeast YS58-12.The alcoholic ferment indicated that the recombinant yeast YS58-12 could convert xylose to ethanol with the xylose consumption rate of 81.3%.展开更多
基金supported by the National High Technology Research and Development Program of China (Nos.2002AA514010,2001AA514024).
文摘Candida shehatae gene xyl1 and Pichia stipitis gene xyl2,encoding xylose reductase(XR)and xylitol dehydrogenase(XD)respectively,were amplified by PCR.The genes xyl1 and xyl2 were placed under the control of promoter GAL in vector pYES2 to construct the recombinant expression vector pYES2-P12.Subsequently the vector pYES2-P12 was transformed into S.cerevisiae YS58 by LiAc to produce the recombinant yeast YS58-12.The alcoholic ferment indicated that the recombinant yeast YS58-12 could convert xylose to ethanol with the xylose consumption rate of 81.3%.
