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Progress of safflower (Carthamus tinctorius L.) regeneration through tissue culture 被引量:9
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作者 FAN Lijiao GUO Meili 《Journal of Medical Colleges of PLA(China)》 CAS 2013年第5期289-301,共13页
Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carri... Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system. 展开更多
关键词 safflower carthamus tinctorius l. REGENERATION Tissue c .ulture
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Color spaces of safflower (Carthamus tinctorius L.) for quality assessment 被引量:5
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作者 Manfei Xu Chenzhao Du +3 位作者 Na Zhang Xinyuan Shi Zhisheng Wu Yanjiang Qiao 《Journal of Traditional Chinese Medical Sciences》 2016年第3期168-175,共8页
Objective:In this study,safflower(Carthamus tinctorius L.)was taken as a representative example to examine the application of color characteristics to evaluate quality.Methods:A computer vision system was established ... Objective:In this study,safflower(Carthamus tinctorius L.)was taken as a representative example to examine the application of color characteristics to evaluate quality.Methods:A computer vision system was established for the objective and nondestructive assessment of color using image processing algorithms.Color parameters were investigated based on the RGB,L*a*b and HSV color spaces.The content of hydroxysafflor yellow A(HSYA),a major bioactive constituent of safflower,was determined by high-performance liquid chromatography.The relationship between HSYA content and color values was investigated by Pearson correlation analysis.A multiple linear regression model was established to predict the HSYA content from color values.Results:The red color and lightness of safflower were found to be significantly related to HSYA content.The prediction equation obtained by multiple regression was reliable with an R2 value of 0.805(P<.01).Conclusion:The results suggest that the computer vision technique could be used as a promising and non-destructive technology for color measurement and quality evaluation of CHM. 展开更多
关键词 Computer vision carthamus tinctorius l. COlOR Quality evaluation
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Safflower (Carthamus Tinctorius L.) a Potential Source of Drugs against Cryptococcal Infections, Malaria and Leishmaniasis 被引量:1
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作者 Aknur Turgumbayeva Gulbaram Ustenova +9 位作者 Ubaidilla Datkhayev Khairolla Rahimov Silvijus Abramavicius Agile Tunaityte Kairat Zhakipbekov Kaldanay Kozhanova Saken Tulemissov Ozikhan Ustenova Gulmira Datkayeva Edgaras Stankevicius 《Phyton-International Journal of Experimental Botany》 SCIE 2020年第1期137-146,共10页
In this research we present that Carthamus Tinctorius L.(gen.Asteraceae,otherwise known as Safflower)(Fig.1)may contain agents active in Cryptococcal infections,malaria and Leishmaniasis,as treatment options are becom... In this research we present that Carthamus Tinctorius L.(gen.Asteraceae,otherwise known as Safflower)(Fig.1)may contain agents active in Cryptococcal infections,malaria and Leishmaniasis,as treatment options are becoming scarce due to drug resistance development.Phytochemistry and pharmacological activities(antimicrobial,antimalarial,antileishmanial)of C.tinctorius L.were analyzed.The composition of volatile oil of safflower dried flowers was analyzed by gas chromatography-mass spectrophotometry with flame ionization detector(GC-FID)and in vitro sensitivity assays were performed to assess biological activity.8 known and 3 unknown compounds were detected in the extract(Fig.1).Then the Safflower ointment was manufactured and its acute toxicity study on rats was tested.The volatile oil of C.tinctorius L exhibited activity against Cryptococcus neoformans,Plasmodium falciparum and Leishmania donovani.Safflower volatile oil has anticryptococcal,antimalarial and antileishmanial effects.The prepared ointment had an excellent acute toxicity safety profile. 展开更多
关键词 carthamus tinctorius l. safflower volatile oil GC-FID biological activity
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Assay for the developmental toxicity of safflower (Carthamus tinctorius L.) to zebrafish embryos/larvae 被引量:1
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作者 Qing Xia Zhiqiang Ma +7 位作者 Xue Mei Jun Luo Yutong Wang Tongtong Li Yaru Feng Yuanyuan Ni Qinwen Zou Ruichao Lin 《Journal of Traditional Chinese Medical Sciences》 2017年第1期71-81,共11页
Objective:To explore the developmental toxicity and the potential toxicological mechanism of safflower (Carthamus tinctorius L.) on zebrafish embryos/larvae.Methods:Mortality,malformations and increased apoptosis indu... Objective:To explore the developmental toxicity and the potential toxicological mechanism of safflower (Carthamus tinctorius L.) on zebrafish embryos/larvae.Methods:Mortality,malformations and increased apoptosis induced by safflower were assessed in zebraflsh embryos from 6 to 96 hours post-fertilization.Enzymes and genes in the anti-oxidative and apoptotic pathways were also assayed.Results:The lethal concentration 50 of safflower to zebrafish embryos was 345.6 mg/L.Hatching inhibition,abnormal spontaneous movement,depressed heart rate,pericardial edema,yolk sac edema,abnormal head-trunk angle,inhibition of melanin release,enlarged yolk,and short body length were observed in safflower-treated zebrafish.Additional apoptotic cells mainly appeared around the heart.Safflower exposure changed the activities of defense enzymes (SOD T,CAT↑,MDA↑,GPX without trend),increased MDA content,decreased caspase-3 activity,and altered mRNA levels of related genes (ogg1 ↓,p53↓,Cu/Zn-sod↑,Mn-sod↓,cat↓,gpx↑).Conclusion:Safflower exhibits developmental toxicity for zebrafish embryos/larvae.The developing heart was speculated as the target organ of toxicity.Oxidative stress and increased apoptosis have roles in the developmental toxicity of safflower.This article provides a novel method to research the teratogenicity and possible mechanisms of toxicity of traditional Chinese medicines that are prohibited or contraindicated in pregnant women. 展开更多
关键词 carthamus tinctorius l. DEVElOPMENTAl toxicity ZEBRAFISH Apoptosis OXIDATIVE stress
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Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i>L.) cv. NARI-H-15
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作者 Jeyachandran Vijayakumar Ponnirul Ponmanickam +2 位作者 P. Samuel D. N. P. Sudarmani J. Pandiarajan 《American Journal of Plant Sciences》 2017年第4期688-705,共18页
The effect of meta-Topolin (mT) was assessed to develop a reliable protocol for efficient plant regeneration of safflower (Carthamus tinctorius L.) cv. NARI-H-15. For micropropagation, 7 - 9 days old shoot-tip explant... The effect of meta-Topolin (mT) was assessed to develop a reliable protocol for efficient plant regeneration of safflower (Carthamus tinctorius L.) cv. NARI-H-15. For micropropagation, 7 - 9 days old shoot-tip explants cultured on MS basal medium supplemented with 3.0 mg/L meta-Topolin (mT) + 0.5 mg/L CPPU showed 97.7% adventitious shoot formation (42.4 shootlets) than node after 45 days of culture. For organogenesis, the seedling explants of immature leaf cultured on 1.5 mg/L CPPU or 1.5 mg/L NAA fortified medium produced high amount of callus than cotyledon and stem calli after 60 days of culture. However, MS basal medium fortified with 4.0 mg/L mT + 1.5 mg/L CPPU was found beneficial to stimulate 100% organogenic response (74.7 shootlets) from immature leaf calli than cotyledon and stem derived calli after 45 days of culture. The healthy plantlets obtained from micropropagation and organogenesis process cultured on 1/4 MS basal salts, 1.5% sucrose (w/v) and 0.8% agar (w/v) medium supplemented with NAA (1.5 mg/L) and mT (0.1 mg/L) produced maximum of 96% (12.8 rootlets) and 84% (7.3 rootlets) adventitious rooting, respectively than mT and CPPU tested medium. However, maximum of 67% and 42% survival rate was noticed when in vitro raised plants from micropropagation and organogenesis were hardened in pots containing soil mix and maintained under green house condition. This optimized regeneration protocol might be helpful in regeneration of new genotypes and cultivars of safflower to improve agronomic traits through in vitro selection process and Agrobacterium-mediated genetic transformation system. 展开更多
关键词 carthamus tinctorius l. Meta-Topolin N-(2-chloro-4-pyridyl-N’-phenylurea) MICROPROPAGATION ORGANOGENESIS
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红花(Carthamus tinctorius L.)种子醇溶蛋白遗传多样性分析 被引量:14
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作者 刘仁建 吴卫 +2 位作者 郑有良 杨玉霞 张丽 《中国油料作物学报》 CAS CSCD 北大核心 2006年第2期109-114,共6页
利用A—PAGE(Acid—polyacrylamide gel electrophoresis)技术对来源于32个国家的53份红花材料进行了种子醇溶蛋白检测。结果表明,这些红花材料具有丰富的醇溶蛋白等位变异,共分离出15条迁移率不同的谱带。其中,每份材料可电泳出5... 利用A—PAGE(Acid—polyacrylamide gel electrophoresis)技术对来源于32个国家的53份红花材料进行了种子醇溶蛋白检测。结果表明,这些红花材料具有丰富的醇溶蛋白等位变异,共分离出15条迁移率不同的谱带。其中,每份材料可电泳出5-12条谱带,平均8.5条,所有材料有1条共有带。红花种子醇溶蛋白可作为评价红花遗传多样性的工具之一。不同材料的遗传相似系数(GS)变异范围为0.375—1.000,平均值为0.752。聚类分析结果表明,在GS值为0.752的水平上供试材料聚为6大类,其亲缘关系远近与地理来源关系不大。其中,来自中国的7份材料分别被聚在了3个大类中,表明中国红花种子醇溶蛋白遗传多样性比较丰富。 展开更多
关键词 红花 醇溶蛋白 遗传多样性 聚类分析 A—PAGE
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SRAP Analysis on Genetic Relationship of Carthamus tinctorius L Varieties in Xinjiang 被引量:1
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作者 刘本文 唐婷婷 +5 位作者 李彦锦 江磊 陈雁 覃瑞 刘虹 李刚 《Agricultural Science & Technology》 CAS 2013年第9期1228-1231,1270,共5页
[Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L(safflower) varieties in Xinjiang, and thus to provide a scientific basis for its protection and development. [Method] Th... [Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L(safflower) varieties in Xinjiang, and thus to provide a scientific basis for its protection and development. [Method] The genomic DNA of five safflower varieties from Xinjiang and one from Yunnan were analyzed and compared by SRAP molecular marker. [Result] Twelve pairs of SRAP primers were selected to amplify the genomic DNA of the six materials. Among the 171 clear DNA bands finally obtained, 93 were polymorphic, accounting for 54.4% of total. Genetic similarity coefficient of the six safflower varieties ranged from 0.60 to 0.92. [Conclusion]SRAP molecular marker is suitable for safflower varieties research and to guide the molecular breeding. 展开更多
关键词 carthamus tinctorius lsafflower Genetic relationship SRAP Cluster analysis
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Molecular mechanism of Salvia miltiorrhiza Bunge and Carthamus tinctorius L. in the treatment of myocardial ischemia based on network pharmacology: a comparative study
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作者 Ronghong Liu Yanping Wang +1 位作者 Wenjing Li Bo Hong 《Asian Journal of Traditional Medicines》 CAS 2022年第2期51-64,共14页
Based on network pharmacology and molecular docking technology,the similarities and differences of anti-myocardial ischemia mechanism between Salvia miltiorrhiza Bunge(SM)and Carthamus tinctorius L.(CT)were studied.Fi... Based on network pharmacology and molecular docking technology,the similarities and differences of anti-myocardial ischemia mechanism between Salvia miltiorrhiza Bunge(SM)and Carthamus tinctorius L.(CT)were studied.Firstly,based on the traditional Chinese medicine systems pharmacology(TCMSP),the related compounds of SM and CT were obtained,and the potential targets of these compounds were collected by the target fishing method.Genecards database was used to obtain targets related to myocardial ischemia.The cross targets of CT,SM,and myocardial ischemia were then selected,and the protein-protein interaction(PPI)network was constructed based on the STRING database.The cross targets were imported into the Metascape database for Gene Oncology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Cytoscape software was used to build the topological network diagram of the drug-compound-target path.Finally,the binding ability of the active ingredient and the key target was verified by molecular docking.65 active ingredients and 38 potential targets were screened from SM,and 22 active ingredients and 58 potential targets were excavated from CT.Important targets common to SM and CT were TNF,IL6,VEGFA,AKT1,etc.The common enrichment pathways involved are fluid shear stress and atherosclerosis,IL-17 signaling pathway,pathways in cancer,and toxoplasmosis.The findings suggested that the two traditional Chinese medicines exerted the effect of myocardial ischemia through the characteristics of multiple targets,multiple pathways,and multiple compounds. 展开更多
关键词 myocardial ischemia Salvia miltiorrhiza Bunge carthamus tinctorius l network pharmacology molecular docking
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油用型红花(Carthamus tinctoris L.)种子醇溶蛋白遗传多样性分析 被引量:7
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作者 官玲亮 吴卫 +2 位作者 郑有良 刘仁建 周宇 《种子》 CSCD 北大核心 2007年第4期6-10,共5页
利用A-PAGE(Acid-polyacrylamide gel electrophoresis)技术对来源于不同国家的79份油用型红花材料醇溶蛋白位点进行检测。结果表明,油用型红花醇溶蛋白位点存在丰富的变异类型,共分离出15条迁移率不同的谱带,每份材料具有3—12条... 利用A-PAGE(Acid-polyacrylamide gel electrophoresis)技术对来源于不同国家的79份油用型红花材料醇溶蛋白位点进行检测。结果表明,油用型红花醇溶蛋白位点存在丰富的变异类型,共分离出15条迁移率不同的谱带,每份材料具有3—12条不等,平均8.5条。材料间平均遗传相似系数(GS)为0.5303,变幅为0.1333—1.000,且各洲间遗传多样性大于洲内遗传多样性。在GS值为0.512的水平上,供试材料聚为六大类,聚类结果表明,红花醇溶蛋白图谱类型与其地理分布有一定的相关,但不显著。 展开更多
关键词 红花 醇溶蛋白 A-PAGE 遗传多样性 聚类分析
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Effects of Plant Population on Growth, Development and Oil Yield of Safflower
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作者 Vallantino Emongor Otsoseng Oagile Boipuso Kedikanetswe 《Journal of Agricultural Science and Technology(B)》 2013年第5期321-333,共13页
Two field trials were clone to evaluate the effects of plant density on the growth, development and yield of safflower. The results showed that plant density and season of growth had significant (P ≤ 0.01) effects ... Two field trials were clone to evaluate the effects of plant density on the growth, development and yield of safflower. The results showed that plant density and season of growth had significant (P ≤ 0.01) effects on growth, development, yield components, yield and oil content of safflower. Increasing safflower plant density from 100,000 to 250,000 plants ha^- significantly reduced plant height (13.2%-21.3%), branch number plant^-1 (37%-54.7%), leaf number plant^-1 (39%-39.2%), leaf area (19.5%-53%), plant spread (39.6%-54.4%), root length (28.1%-54.4%), plant biomass (17%-50%), capitula size (12%-12.7%), capitula number plant^-1 (39.5%-50.5%), seed number capitula~ (39%-45%), capitula weight (3.3%-3.6%), seed yield (67.9%-69.8%) and seed oil content (14.7%-20.8%). The reduction in vegetative growth, yield components, yield and oil content of safflower due to increased plant density was attributed to inter and intra-plant competition for light, nutrients and water necessary for growth and development. The differences between winter and summer grown safflower were attributed to difference in day and night temperature (DIF) and the average daily temperature which were optimum for safflower growth in winter. It was concluded that under Botswana conditions or in semi-arid areas, safflower should be planted at 50 cm × 20 cm or wider in order to maximize yield and oil content and allow the plants to express their maximum genetic potential. 展开更多
关键词 safflower carthamus tinctorius l. plant density vegetative growth yield and yield components oil content.
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The Main Insect Pests of Safflower on Various Plant Parts in Iran
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作者 Mehdi Nasr Esfahani Giti Alizadeh Zahra Zarei Mohamad Nasr Esfahani 《Journal of Agricultural Science and Technology(A)》 2012年第11期1281-1288,共8页
Safflower (Carthamus tinctorius) has its own limited factors, especially insect pests. There are several injurious insect pests reported from various safflower growing areas in Iran, causing severe and economical lo... Safflower (Carthamus tinctorius) has its own limited factors, especially insect pests. There are several injurious insect pests reported from various safflower growing areas in Iran, causing severe and economical losses on various parts of safflower plants. They are safflower Budfly and capsulfly (Acanthiophilus helianthi), aphid (Uroleucon carthami), green leafhopper (Empoasca decipiens), ground bug (Oxycarneus pallens), grampod borer and capsule borer or bordered straw (Heliothis (and or Heliocaverpa) peltigera), red spider mite and two spotted spider mite (Tetranychus urtica), beet armyworm (Spodoptera exigua), scarab beetle (Tropinota (and or Epicometis) hirta), Egyptian cotton leaf (Spodoptera littoralis), cotton boll worm (Heliothi absouleta) and so on. The other reported safflower insect pests from other countries are wireworms (Limonius spp.), lygus bugs (Lygus hesperus), flower thrips (Eastern or Western) or onion thrips (Thrips tabaci), and seed corn maggots (Delia platura), sunflower moths (Helianthus annuus) and Caterpillar (Perigaea capensis) are the serious pests of this crop. The other reports showed that some stored-product insects are capable of establishing infestations in safflower seeds and seed meal under suitable conditions. Only Lasiaderma serricorne, Stegobium penliceum, and the four species of Trogodema were to be as the infectious pests. Safflower is also attractive to several beneficial insects, including lady beetles and lacewings that may provide insect control in surrounding fields. 展开更多
关键词 safflower carthamus tinctorius l. INSECTS PESTS Iran.
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Reproductive Components of Safflower Genotypes Submitted of Bulk Density Levels in the Brazilian Cerrado
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作者 Juliana Terezinha Sasso Paludo Edna Maria Bonfim-Silva +3 位作者 Tonny José Araújo da Silva Maurício Dutra Zanotto William Fenner Marcio Koetz 《American Journal of Plant Sciences》 2017年第9期2069-2082,共14页
Nutrient absorption in crops can decline and their development can be hindered by increased bulk density. This study aimed at assessing the manner in which bulk density levels affect the reproductive structures of the... Nutrient absorption in crops can decline and their development can be hindered by increased bulk density. This study aimed at assessing the manner in which bulk density levels affect the reproductive structures of the safflower genotypes in the Brazilian Cerrado. The completely randomized design was adopted with four replications for the experiment, which was conducted in a greenhouse using Oxisol collected from 0.0 to 0.2 m depth from the region supporting Cerrado vegetation. The treatments included ten safflower genotypes (PI 237538, PI 248385, PI 250196, PI 301049, PI 305173, PI 305205, PI 306520, PI 306603, PI 560202 and PI 613366) and five bulk density levels (1.0, 1.2, 1.4, 1.6 and 1.8 Mg·m-3). Evaluations were done at 90 days after emergence, in terms of the number, diameter and dry mass of the heads. The data were submitted to the analysis of variance. The means were grouped using the Scott-Knott test at 5% probability. The diameter and dry mass of the chapters were influenced by the mean bulk density of 1.10 Mg·m-3. A notable interaction was evident between the safflower genotypes and bulk density levels for the diameter and dry mass of the head alone, revealing the high degree of genetic variability that environmental changes induce among the genotypes. The PI 250196, PI 301049, PI 305173 and PI 305205 genotypes exhibited greater stability to the bulk density variations compared with the others. Mean bulk density of 1.2 Mg·m-3 was found to impair the development of the reproductive components of the safflower genotypes. 展开更多
关键词 carthamus tinctorius l. BUlK Density Oleaginous CROP safflower GENOTYPES Physical Attributes of Soil
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Phytometric and Productive Characteristics of Safflower Submitted to Phosphate Fertilization in the Oxisol of the Brazilian Cerrado
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作者 Edna Maria Bonfim-Silva Luiz Fernando Santos Miranda +2 位作者 Laura Cristina Rezende das Neves Helon Hébano de Freitas Sousa Jefferson Vieira José 《American Journal of Plant Sciences》 2017年第12期2966-2976,共11页
The safflower (Carthamus tinctorius L.), in particular, has been soaring in significance as an alternative for oil and biodiesel production. In this context, the goal of this study was to assess the influence exerted ... The safflower (Carthamus tinctorius L.), in particular, has been soaring in significance as an alternative for oil and biodiesel production. In this context, the goal of this study was to assess the influence exerted by phosphate fertilization on the phytometric and productive characteristics of the safflower raised on the Oxisol of the Cerrado. The experiment was performed in a greenhouse, using pots of 3 dm-3?in volume. Adopting the completely randomized design, five doses of phosphorus (P2O5: 0, 90, 180, 360 and 540 mg·dm-3) and six replicates were included. The following variables were estimated, viz., number of leaves, plant height, and stem diameter, besides chlorophyll index (SPAD index), number of chapters and shoot dry matter. Barring the shoot dry matter and number of chapters, all the other variables were evaluated on days 30 and 45 after the plants were transplanted. Data were submitted to analysis of variance at 1%, 5% and 10% probabilities, and regression analysis, at 1% and 5% probabilities, respectively. All the variables revealed significant differences and adjustments to the linear regression model (P < 0.1), excepting the SPAD index on day 30 after the plant emergence which was adjusted to the quadratic regression model. The highest findings of 24.47 for leaf number, 34.97 cm for plant height, 3.76 mm for stem diameter, 64.76 for chlorophyll index, 5 units for the number of chapters and 3.45 g for dry shoot mass were recorded in response to the highest phosphorus dose in the experimental range (P2O5: 540 mg·dm-3). Phosphate fertilization was observed to affect the phytometric characteristics and boost the development of the safflower plants cultivated in the Cerrado Oxisol, substantiating that safflower could be a viable cash crop that can be established in the Brazilian Cerrado. 展开更多
关键词 carthamus tinctorius l. Alternative Culture for BIODIESEl Oleaginous
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Soil Water Availability on Growth and Development of Safflower Plants
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作者 Edna Maria Bonfim-Silva Ellen Cristina Alves de Anicésio +2 位作者 Jakeline Rosa de Oliveira Helon Hébano de Freitas Sousa Tonny José Araújo da Silva 《American Journal of Plant Sciences》 2015年第13期2066-2073,共8页
Safflower (Carthamus tinctorius L.) is a promising culture to be widespread in Brazil. However, the lack of basic knowledge about cultivation techniques, such as water demand by the culture, is still obstacle to the e... Safflower (Carthamus tinctorius L.) is a promising culture to be widespread in Brazil. However, the lack of basic knowledge about cultivation techniques, such as water demand by the culture, is still obstacle to the expansion of safflower in that country. The objective was, then, to evaluate the effect of the soil water availability on growth and development of safflower in the Cerrado soil of Mato Grosso, Brazil. The experiment was conducted in a greenhouse, in a completely randomized design with five water availabilities (25%, 50%, 75%, 100% and 125% of the maximum water holding capacity in the soil) and four replications. Maintenance soil moisture was performed by gravimetric method with daily weighing of experimental units. The variables analyzed were: plant height, stem diameter, number of leaves, number of heads, heads diameter, dry mass of shoots, heads, and roots. The results were submitted to analysis of variance and regression test at 5% probability by SISVAR program. All variables set to the quadratic regression model, showing the best results in the water availability between 64% and 76%. Safflower is shown to be more sensitive to water stress with increased tolerance to water deficit in the soil than to flooding. 展开更多
关键词 carthamus tinctorius l. FIElD Capacity WATER STRESS IMA 0213
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Soil Water Availabilities in the Content and Accumulation of Nitrogen and Chlorophyll Index in the Safflower
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作者 Edna Maria Bonfim-Silva Ellen Cristina Alves de Anicésio +2 位作者 Jakeline Rosa de Oliveira Helon Hébano de Freitas Sousa Tonny José Araújo da Silva 《American Journal of Plant Sciences》 2015年第14期2280-2286,共7页
The safflower (Carthamus tinctorius L.) is adapted to grow in adverse conditions, is tolerant to salinity and water deficit, however, its productivity increases when grown in areas with greater availability of water a... The safflower (Carthamus tinctorius L.) is adapted to grow in adverse conditions, is tolerant to salinity and water deficit, however, its productivity increases when grown in areas with greater availability of water and in fertilized conditions, showing that the knowledge of crop management can bring income gains for producers. It is known that extreme conditions of soil moisture damage the crop development. Given this context, the objective was to evaluate the influence of water availability on the nutritional characteristics of the safflower. The experiment was conducted in a greenhouse in a completely randomized design with five water availabilities (25%, 50%, 75%, 100% and 125% of the maximum water holding capacity of the soil) in four replications. Maintaining soil moisture was performed by gravimetric method with daily weighing of experimental units. The variables analyzed were SPAD reading (chlorophyll content), concentration and accumulation of nitrogen in the shoot and in the sections. The results were submitted to analysis of variance and regression test at 5% probability by Sisvar program. There was adjusting of variables to linear and quadratic regression models. Water availabilities between 65% and 75% have greater potential to promote better nutrition for safflower plants. Both deficit and excess of water in the soil are detrimental to the absorption of nitrogen and reduce the chlorophyll content. Safflower is less tolerant to excess than to deficit of water. 展开更多
关键词 carthamus tinctorius l. NUTRITION SPAD IRRIGATION
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Potential for Safflower Cultivation under Temperate Conditions
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作者 Jehad Abbadi Joska Gerendas 《Journal of Agricultural Science and Technology(A)》 2012年第12期1307-1321,共15页
Safflower is a Mediterranean-subtropical oil crop, with temperate climate. To assess the suitability of safflower may have certain production potential in organic farming in regions cultivation under temperate climate... Safflower is a Mediterranean-subtropical oil crop, with temperate climate. To assess the suitability of safflower may have certain production potential in organic farming in regions cultivation under temperate climate in low input organic farming, ten cultivars were grown in two years experiment in Lindhoft-Kiel in Northern Germany in clay/sand soil. Pearson correlations reveal that oil yield exhibited tight positive correlations with plant height, plant dry matter, capitula plant-1, achenes capitulum-1 and thousand achene mass (TAM), but was not correlated with days to full flowering or oil concentration. Yield component analysis reveals that achenes capitulum1 had the major influence on the variation of oil yield of all studied cultivars, followed by capitula plant1 and TAM, while the oil concentration had slightly negative influence, and the plant density was negatively associated with oil yield. Although two years field experiment is not enough to analyse the performance of safflower adaptability to certain region, it can be concluded that the most adaptive cultivars are PI-209286 originated from Romania, and cultivar CART-19/89 originated from Poland, while the less adaptive cultivars were DO-13/03 and DO-15/03 with German origin in addition to the Canadian cultivar PI-572475/Saffire. The most striking yield component that contributes to the high yield is achene capitulumt, which can be used as a base for selecting cultivars adaptive to humid cold conditions. The growth period between full flowering and harvest influencing this yield component must be given attention to avoid low temperature and rainfall by tuning sowing date to improve yield at these conditions. 展开更多
关键词 carthamus tinctorius safflower production organic farming temperate climate ADAPTATION genotype.
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Simultaneous determination of four nucleosides in Carthamus tinctorius L.and Safflower injection using high-performance liquid chromatography 被引量:1
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作者 周斐然 赵明波 屠鹏飞 《Journal of Chinese Pharmaceutical Sciences》 CAS 2009年第4期326-330,共5页
We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column usin... We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column using a gradient elution with mobile phases of 0.05% trifluoroacetic acid (TFA) aqueous solution (A) and methanol (B). The assay was carried out at a flow rate of 1 mL/min at 25 ℃ with detection at 260 nm. Cytidine, uridine, adenosine and guanosine showed good linearity in the ranges of4.02-503μg/mL (r2= 0.9998), 9.38-1407 μg/mL (rz = 0.9999), 80.6-8060μg/mL (r2 = 0.9999) and 2.10---630μg/mL (r2 = 0.9987) with average recoveries of 97.2%, 94.5%, 98.6% and 108.6%, respectively. The contents of cytidine, uridine, adenosine and guanosine in different Carthamus tinctorius L. and Safflower injection were significantly different. This is the first report on the quantitative determination of nucleosides in Carthamus tinctorius L. and Safflower injection. 展开更多
关键词 carthamus tinctorius l. safflower injection NUClEOSIDE High-performance liquid chromatography
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Cloning,expression and activity analysises of chalcone synthase genes in Carthamus tinctorius
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作者 Xiaohui Tang Chaoxiang Ren +6 位作者 Jing Hu Jiang Chen Jie Wang Rui Wang Qinghua Wu Wan Liao Jin Pei 《Chinese Herbal Medicines》 CAS 2023年第2期291-297,共7页
Objective:Flavonoids are the bioactive compounds in safflower(Carthamus tinctorius),in which chalcone synthase(CHS)is the first limiting enzyme.However,it is unclear that which chalcone synthase genes(CHSs)are partici... Objective:Flavonoids are the bioactive compounds in safflower(Carthamus tinctorius),in which chalcone synthase(CHS)is the first limiting enzyme.However,it is unclear that which chalcone synthase genes(CHSs)are participated in flavonoids biosynthesis in C.tinctorius.In this study,the CHSs in the molecular characterization and enzyme activities were investigated.Methods:Putative chalcone biosynthase genes were screened by the full-length transcriptome sequences data in C.tinctorius.Chalcone biosynthase genes in C.tinctorius(CtCHSs)were cloned from cDNA of flowers of C.tinctorius.The cloned gene sequences were analyzed by bioinformatics,and their expression patterns were analyzed by real-time PCR(RT-PCR).The protein of CtCHS in the development of flowers was detected by polyclonal antibody Western blot.A recombinant vector of CtCHS was constructed.The CtCHS recombinant protein was induced and purified to detect the enzyme reaction(catalyzing the reaction of p-coumaryl-CoA and malonyl-CoA to produce naringin chalcone).The reaction product was detected by HPLC and LC-MS.Results:Two full-length CtCHS genes were successfully cloned from the flowers of safflower(CtCHS1 and CtCHS3),with gene lengths of 1525 bp and 1358 bp,respectively.RT-PCR analysis showed that both genes were highly expressed in the flowers,but the expression of CtCHS1 was higher than that of CtCHS3 at each developmental stage of the flowers.WB analysis showed that only CtCHS1 protein could be detected at each developmental stage of the flowers.HPLC and LC-MS analyses showed that CtCHS1 could catalyze the conversion of p-coumaryl-CoA and malonyl-CoA substrates to naringin chalcone.Conclusion:CtCHS1 is involved in the biosynthesis of naringin chalcone in safflower. 展开更多
关键词 carthamus tinctorius l. chalcone synthase expression analysis FlAVONOIDS functional identification gene cloning safflower
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不同品种红花HPLC指纹图谱及其化学成分差异性研究 被引量:1
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作者 黄亚威 周慧娟 +4 位作者 刘旭云 王沛琦 胡尊红 杨谨 胡学礼 《中成药》 CAS CSCD 北大核心 2023年第1期155-160,共6页
目的采用HPLC指纹图谱结合化学模式识别评价不同品种红花Carthamus tinctorius L.质量。方法建立HPLC指纹图谱,采用HPLC-Q-TOF-MS法对共有峰进行鉴定,结合聚类分析、偏最小二乘判别分析进行质量评价,筛选差异成分。结果43批样品指纹图... 目的采用HPLC指纹图谱结合化学模式识别评价不同品种红花Carthamus tinctorius L.质量。方法建立HPLC指纹图谱,采用HPLC-Q-TOF-MS法对共有峰进行鉴定,结合聚类分析、偏最小二乘判别分析进行质量评价,筛选差异成分。结果43批样品指纹图谱中有12个共有峰,鉴定出6-羟基山柰酚-3,6,7-三-O-β-D-葡萄糖苷、烟花苷等11种成分,相似度为0.919~0.998。不同品种样品聚为3类,9,12,13-三羟基-10-十八碳烯酸等6种成分为质量差异标志物。结论该方法可有效识别不同品种红花成分差异,为该药材选育及质量控制提供依据。 展开更多
关键词 红花 HPlC指纹图谱 品种 化学成分 HPlC-Q-TOF-MS 聚类分析 偏最小二乘判别分析
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化肥减量配施微生物菌肥对膜下滴灌红花生长发育及产量的影响
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作者 贾东海 宋贤明 +5 位作者 顾元国 李强 曾幼玲 苗昊翠 郭美丽 侯献飞 《新疆农业科学》 CAS CSCD 北大核心 2024年第4期781-790,共10页
【目的】研究化肥减量配施微生物菌肥对膜下滴灌红花生长发育及产量的影响。【方法】2020~2021年在新疆塔城地区裕民县设置2年定位施肥试验,采用裂区试验设计,设6个处理:(1)对照CK:不施肥;(2)CF:复合肥20 kg/667m^(2);(3)OF:微生物菌肥2... 【目的】研究化肥减量配施微生物菌肥对膜下滴灌红花生长发育及产量的影响。【方法】2020~2021年在新疆塔城地区裕民县设置2年定位施肥试验,采用裂区试验设计,设6个处理:(1)对照CK:不施肥;(2)CF:复合肥20 kg/667m^(2);(3)OF:微生物菌肥20 kg/667m^(2);(4)CF+25M:化肥15 kg/667m^(2)(化肥减量25%)+微生物菌肥5 kg/667m^(2);(5)CF+37.5M:化肥12.5 kg/667m^(2)(化肥减量37.5%)+微生物菌肥7.5 kg/667m^(2);(6)CF+50M:化肥10 kg/667m^(2)(化肥减量50%)+微生物菌肥10 kg/667m^(2)。研究不同施肥处理对红花农艺性状、干物质积累与分配和产量形成的影响。【结果】处理CF+25M和CF+37.5M有利于红花的生长发育,可以显著增加红花株高、分枝数、叶片数和叶绿素含量等,并能促进红花干物质积累,调节干物质分配,协调改善红花产量构成因素,从而增加红花花丝和籽粒产量。其中以CF+37.5M处理下红花综合性状表现最优,其单株果球数、千粒重、花丝产量和籽粒产量分别比CF处理显著提高了87.85%、12.29%、11.42%和15.78%,同时株高、根长和叶绿素含量亦均达到最高水平。【结论】化肥减量配施微生物菌肥CF+37.5M:化肥12.5 kg/667m^(2)(化肥减量37.5%)+微生物菌肥7.5 kg/667m^(2)最优,能有效降低化肥施用量,促进红花生长发育,从而提高肥料利用率。 展开更多
关键词 红花 化肥减量 微生物菌肥 干物质积累 生长 产量
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