[Objective] The paper was to study the effects of exogenous GA3 on seed germination and seedling growth of Salicornia europaea L.under salt stress.[Method] With the combination of different concentrations of NaCl（50,...[Objective] The paper was to study the effects of exogenous GA3 on seed germination and seedling growth of Salicornia europaea L.under salt stress.[Method] With the combination of different concentrations of NaCl（50,100,150,200,250,300,350,400 mmol/L）with 10 μmol /L GA3 treatments,the effects of exogenous GA3 on seed germination and seedling growth of S.europaea under salt stress were studied.[Result] Exogenous GA3 promoted the seed germination rate,germination capacity,germination index and seed vigor index of S.europaea under salt stress.Under the treatment of exogenous GA3,the root system vigor,the lengths of roots and stems and the salt-tolerance of S.europaea were higher than that of single salt stress treatment.[Conclusion] Within a certain concentration range,exogenous GA3 could alleviate the inhibition effects of salt stress on seed germination and seedling growth of S.europaea.展开更多
Objective:To investigate the pharmacodynamic material basis,mechanism of actions and targeted diseases of Salicornia europaea L.(SE)based on the network pharmacology method,and to verify the antidepressant-like effect...Objective:To investigate the pharmacodynamic material basis,mechanism of actions and targeted diseases of Salicornia europaea L.(SE)based on the network pharmacology method,and to verify the antidepressant-like effect of the SE extract by pharmacological experiments.Methods:Retrieval tools including Chinese medicine(CM),PubMed,PharmMapper,MAS 3.0 and Cytoscape were used to search the components of SE,predict its targets and related therapeutic diseases,and construct the"Component-TargetPathway"network of SE for central nervous system(CNS)diseases.Further,protein-protein interaction(PPI)network,Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)function annotation of depression-related targets were analyzed to predict the antidepressant mechanism of SE.Chronic unpredictable mild stress(CUMS)model was used to construct a mouse model with depression-like symptoms.And the animals were randomly divided into 6 groups(n=10)including the normal group(nonstressed mice administered with distilled water),the CUMS group(CUMS mice administered with distilled water),the venlafaxine group(CUMS mice administered with venlafaxine 9.38 mg/kg),SE high-,medium-,and low-dose groups(CUMS mice administered with SE 1.8,1.35 and 0.9 g/kg,respectively).Then some relevant indicators were determined for experimental verification by the forced swim test(FST),the tail suspension test(TST)and open-field test(OFT).Dopamine(DA)concentration in hippocampus and cerebral cortex,IL-2 and corticosterone(CORT)levels in blood,and nuclear factor E2 related factor 2(Nrf2),kelch-like epichlorohydrin related protein 1(Keap1),NAD(P)H dehydrogenase[quinone]1(NQO1)and heme oxygenase-1(HO-1)levels in mice were measured by enzyme linked immunosorbent assay(ELISA)and Western blot respectively to explore the possible mechanisms.Results:The"target-disease"network diagram predicted by network pharmacology,showed that the potential target of SE involves a variety of CNS diseases,among which depression accounts for the majority.The experimental results showed that SE(1.8,1.35 g/kg)significantly decreased the immobility period,compared with the CUMS group in FST and TST in mice after 3-week treatment,while SE exhibited no significant effect on exploratory behavior in OFT in mice.Compared with CUMS group,the SE group(0.9 g/kg)showed significant differences(P<0.05)in DA levels in the hippocampus and cerebral cortex.In addition,compared with CUMS control group,SE(1.8 g/kg)group showed a significant effect on decreasing the activities of CORT(P<0.05),and serum IL-2level with no statistical significance.Finally,Western blot results showed that compared with the model group,Nrf2,Keap1,NQO1 and HO-1 protein expressions in SE group(1.8 g/kg)were up-regulated(all P<0.01).Conclusion:The SE extract may have an antidepressant effect,which appeared to regulate Nrf2-ARE pathway and increased levels of DA and CORT in the hippocampus and cortex.展开更多
基金Supported by General Projects of Basic Research of Natural Sciencein Jiangsu Universities(KJD180118)Excellent Course Construction Project of Plant Physiology in Jiangsu Province(2010)+1 种基金Innovative Team Building and Personnel Training Programs in Zhejiang Science and Technology Department(2010F20007)Outstanding Teaching Team Project in Nanjing Xiaozhuang College(4085024)~~
文摘[Objective] The paper was to study the effects of exogenous GA3 on seed germination and seedling growth of Salicornia europaea L.under salt stress.[Method] With the combination of different concentrations of NaCl（50,100,150,200,250,300,350,400 mmol/L）with 10 μmol /L GA3 treatments,the effects of exogenous GA3 on seed germination and seedling growth of S.europaea under salt stress were studied.[Result] Exogenous GA3 promoted the seed germination rate,germination capacity,germination index and seed vigor index of S.europaea under salt stress.Under the treatment of exogenous GA3,the root system vigor,the lengths of roots and stems and the salt-tolerance of S.europaea were higher than that of single salt stress treatment.[Conclusion] Within a certain concentration range,exogenous GA3 could alleviate the inhibition effects of salt stress on seed germination and seedling growth of S.europaea.
基金Supported by the National Nature Science Foundation of China(No.81173530)Tianjin Research Program of Applied Basic and Cutting-edge Technologies(No.17JCZDJC33200 and No.12JCZDJC25900)。
文摘Objective:To investigate the pharmacodynamic material basis,mechanism of actions and targeted diseases of Salicornia europaea L.(SE)based on the network pharmacology method,and to verify the antidepressant-like effect of the SE extract by pharmacological experiments.Methods:Retrieval tools including Chinese medicine(CM),PubMed,PharmMapper,MAS 3.0 and Cytoscape were used to search the components of SE,predict its targets and related therapeutic diseases,and construct the"Component-TargetPathway"network of SE for central nervous system(CNS)diseases.Further,protein-protein interaction(PPI)network,Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)function annotation of depression-related targets were analyzed to predict the antidepressant mechanism of SE.Chronic unpredictable mild stress(CUMS)model was used to construct a mouse model with depression-like symptoms.And the animals were randomly divided into 6 groups(n=10)including the normal group(nonstressed mice administered with distilled water),the CUMS group(CUMS mice administered with distilled water),the venlafaxine group(CUMS mice administered with venlafaxine 9.38 mg/kg),SE high-,medium-,and low-dose groups(CUMS mice administered with SE 1.8,1.35 and 0.9 g/kg,respectively).Then some relevant indicators were determined for experimental verification by the forced swim test(FST),the tail suspension test(TST)and open-field test(OFT).Dopamine(DA)concentration in hippocampus and cerebral cortex,IL-2 and corticosterone(CORT)levels in blood,and nuclear factor E2 related factor 2(Nrf2),kelch-like epichlorohydrin related protein 1(Keap1),NAD(P)H dehydrogenase[quinone]1(NQO1)and heme oxygenase-1(HO-1)levels in mice were measured by enzyme linked immunosorbent assay(ELISA)and Western blot respectively to explore the possible mechanisms.Results:The"target-disease"network diagram predicted by network pharmacology,showed that the potential target of SE involves a variety of CNS diseases,among which depression accounts for the majority.The experimental results showed that SE(1.8,1.35 g/kg)significantly decreased the immobility period,compared with the CUMS group in FST and TST in mice after 3-week treatment,while SE exhibited no significant effect on exploratory behavior in OFT in mice.Compared with CUMS group,the SE group(0.9 g/kg)showed significant differences(P<0.05)in DA levels in the hippocampus and cerebral cortex.In addition,compared with CUMS control group,SE(1.8 g/kg)group showed a significant effect on decreasing the activities of CORT(P<0.05),and serum IL-2level with no statistical significance.Finally,Western blot results showed that compared with the model group,Nrf2,Keap1,NQO1 and HO-1 protein expressions in SE group(1.8 g/kg)were up-regulated(all P<0.01).Conclusion:The SE extract may have an antidepressant effect,which appeared to regulate Nrf2-ARE pathway and increased levels of DA and CORT in the hippocampus and cortex.
