目的对一起因食用肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件进行溯源分析。方法对8例患者进行流行病学调查,采集相关环境、食品及粪便等样本,根据国家标准进行实验室检测,经增菌后挑取可疑菌落进行生化鉴定,对检出的6株沙门...目的对一起因食用肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件进行溯源分析。方法对8例患者进行流行病学调查,采集相关环境、食品及粪便等样本,根据国家标准进行实验室检测,经增菌后挑取可疑菌落进行生化鉴定,对检出的6株沙门氏菌株采用标准诊断血清进行血清凝集试验,药敏试验及脉冲场凝胶电泳。结果该起事件发病8例,6份样本中,分离出肠炎沙门氏菌,沙门氏菌检出率食品、环境和病人粪便样本均为100%,药敏试验结果显示,耐药谱为多粘菌素E(Colistin,CT)―头孢噻肟(Cefotaxime,CTX)―四环素(Tetracycline,TET)―环丙沙星(Ciprofloxacin,CIP)―氨苄西林(Ampicillin,AMP)―氨苄西林/舒巴坦(Ampicillin/Sulbactam,AMS),均呈现6重耐药现象,对氯霉素(Chloramphenicol,CHL)、复方新诺明(Sulfamethoxazole,SXT)、厄他培南(Ertapenem,ETP)、美罗培南(Meropenem,MEM)、头孢他啶(Ceftazidime,CAZ)、头孢他啶/阿维巴坦(Ceftazidime/avibactam,CZA)、替加环素(Tigecycline,TIG)、阿奇霉素(Azithromycin,AZM)、阿米卡星(Amikacin,AMI)敏感,6株菌的脉冲场凝胶电泳(Pulse Field Gel Electrophoresis,PFGE)条带一致,同源性100%,显示为同一克隆。结论该起事件为市民食用被肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件。展开更多
Objective To characterize the diarrheal patients with Salmonella typhimurium （S. typhimurium） infections and to set up the first baseline for S. typhimuri_um pulsed-field gel electrophoresis （PFGE） patterns in Hen...Objective To characterize the diarrheal patients with Salmonella typhimurium （S. typhimurium） infections and to set up the first baseline for S. typhimuri_um pulsed-field gel electrophoresis （PFGE） patterns in Henan province, thus laying a foundation for comprehensive surveillance of Salmonella in human as well as foods. Methods S. typhimurium isolates recovered from outpatients with diarrhea in Henan province from May to October of 2006 were characterized. Antimicrobial susceptibility tests of 8 antimicrobial agents and PFGE were carried out to analyze the S. typhimurium isolates. Results Twenty-four （0.9%） S. typhimurium isolates were identified from 2661 stool specimens of diarrheal cases. Eighty-eight percent of isolates showed resistance to at least one antimicrobial agent. The resistance to chloramphenicol （79%） was most common. Fifty-eight percent of isolates were resistant to ciprofloxacin. All the 14 ciprofloxacin-resistant isolates were resistant to more than five antimicrobial agents. Thirty-three percent of S. typhimurium isolates were resistant to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline （R-type ACSSuT）. Eight antimicrobia-resistant phenotypes were found among the 24 isolates in 16 PFGE patterns. Conclusion The rate of multidrug-resistant S. typhimurium is relatively high in S. typhimurium PFGE patterns of Henan province. Multidrug-resistant S. typhimurium should be considered a public health threat.展开更多
目的了解云南省沙门菌患者中分离最多的肠炎沙门菌及食品中分离最多的德尔卑沙门菌脉冲场凝胶电泳(pulse field gel electrophoresis,PFGE)分子分型及耐药状况。方法参照中国细菌性传染病分子分型实验室监测网络Pulse Net China的沙门菌...目的了解云南省沙门菌患者中分离最多的肠炎沙门菌及食品中分离最多的德尔卑沙门菌脉冲场凝胶电泳(pulse field gel electrophoresis,PFGE)分子分型及耐药状况。方法参照中国细菌性传染病分子分型实验室监测网络Pulse Net China的沙门菌PFGE分子分型方法进行分子分型。分析耐药板最低抑菌浓度(minimal inhibitory concentration,MIC)值,根据美国临床实验室标准化协会(Clinical and Laboratory Standard Institute,CLSI)的相应标准获得S、I、R结果。结果56株肠炎沙门菌呈11种PFGE带型,有4种优势带型,对氨苄舒的耐药率最高,为57.14%,对亚胺培南最敏感。27株德尔卑沙门菌呈25种PFGE带型,对复合磺胺的耐药率最高,为64.29%,对亚胺培南最敏感。结论肠炎沙门菌分子分型有明显的优势带型,本地区肠炎沙门菌对氨苄舒的耐药率最高。德尔卑沙门菌分子分型呈多样分布,复方磺胺是本地区德尔脾沙门菌最耐药抗生素。2种沙门菌都对亚胺培南最敏感。展开更多
目的通过脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)技术分析一起食物中毒事件病原菌的相关性,从遗传学确定该起事件发生的原因。方法参照《感染性腹泻诊断标准》(WS 271—2007),对采集的肛拭子和可疑食物等样品进行病原...目的通过脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)技术分析一起食物中毒事件病原菌的相关性,从遗传学确定该起事件发生的原因。方法参照《感染性腹泻诊断标准》(WS 271—2007),对采集的肛拭子和可疑食物等样品进行病原菌培养、分离和鉴定。利用脉冲场凝胶电泳对分离菌株进行分子分型,采用Bio Numerics 5.1软件开展相关性分析。药敏试验的方法为微量肉汤稀释法。结果本起食物中毒事件共分离到4株肠炎沙门菌,分别分离自患者肛拭子(2株)、厨师肛拭子(1株)与可疑食物(1株)。4株分离菌对7种抗生素存在不同程度的耐药性;PFGE溯源性分析结果显示,4株分离菌呈现高度同源。结论本起食物中毒事件由食用被肠炎沙门菌污染的食物所致。分离株的多重耐药性应引起相关部门的重视。PFGE分子分型从全基因组的层面分析菌株的遗传学关系,是病原菌溯源研究的重要手段。展开更多
文摘目的对一起因食用肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件进行溯源分析。方法对8例患者进行流行病学调查,采集相关环境、食品及粪便等样本,根据国家标准进行实验室检测,经增菌后挑取可疑菌落进行生化鉴定,对检出的6株沙门氏菌株采用标准诊断血清进行血清凝集试验,药敏试验及脉冲场凝胶电泳。结果该起事件发病8例,6份样本中,分离出肠炎沙门氏菌,沙门氏菌检出率食品、环境和病人粪便样本均为100%,药敏试验结果显示,耐药谱为多粘菌素E(Colistin,CT)―头孢噻肟(Cefotaxime,CTX)―四环素(Tetracycline,TET)―环丙沙星(Ciprofloxacin,CIP)―氨苄西林(Ampicillin,AMP)―氨苄西林/舒巴坦(Ampicillin/Sulbactam,AMS),均呈现6重耐药现象,对氯霉素(Chloramphenicol,CHL)、复方新诺明(Sulfamethoxazole,SXT)、厄他培南(Ertapenem,ETP)、美罗培南(Meropenem,MEM)、头孢他啶(Ceftazidime,CAZ)、头孢他啶/阿维巴坦(Ceftazidime/avibactam,CZA)、替加环素(Tigecycline,TIG)、阿奇霉素(Azithromycin,AZM)、阿米卡星(Amikacin,AMI)敏感,6株菌的脉冲场凝胶电泳(Pulse Field Gel Electrophoresis,PFGE)条带一致,同源性100%,显示为同一克隆。结论该起事件为市民食用被肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件。
基金supported by the World Health Organization Global Salm-Surv as part of China-US Collaborative Program on Emerging & Re-emerging Infectious Diseases
文摘Objective To characterize the diarrheal patients with Salmonella typhimurium （S. typhimurium） infections and to set up the first baseline for S. typhimuri_um pulsed-field gel electrophoresis （PFGE） patterns in Henan province, thus laying a foundation for comprehensive surveillance of Salmonella in human as well as foods. Methods S. typhimurium isolates recovered from outpatients with diarrhea in Henan province from May to October of 2006 were characterized. Antimicrobial susceptibility tests of 8 antimicrobial agents and PFGE were carried out to analyze the S. typhimurium isolates. Results Twenty-four （0.9%） S. typhimurium isolates were identified from 2661 stool specimens of diarrheal cases. Eighty-eight percent of isolates showed resistance to at least one antimicrobial agent. The resistance to chloramphenicol （79%） was most common. Fifty-eight percent of isolates were resistant to ciprofloxacin. All the 14 ciprofloxacin-resistant isolates were resistant to more than five antimicrobial agents. Thirty-three percent of S. typhimurium isolates were resistant to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline （R-type ACSSuT）. Eight antimicrobia-resistant phenotypes were found among the 24 isolates in 16 PFGE patterns. Conclusion The rate of multidrug-resistant S. typhimurium is relatively high in S. typhimurium PFGE patterns of Henan province. Multidrug-resistant S. typhimurium should be considered a public health threat.
文摘目的了解云南省沙门菌患者中分离最多的肠炎沙门菌及食品中分离最多的德尔卑沙门菌脉冲场凝胶电泳(pulse field gel electrophoresis,PFGE)分子分型及耐药状况。方法参照中国细菌性传染病分子分型实验室监测网络Pulse Net China的沙门菌PFGE分子分型方法进行分子分型。分析耐药板最低抑菌浓度(minimal inhibitory concentration,MIC)值,根据美国临床实验室标准化协会(Clinical and Laboratory Standard Institute,CLSI)的相应标准获得S、I、R结果。结果56株肠炎沙门菌呈11种PFGE带型,有4种优势带型,对氨苄舒的耐药率最高,为57.14%,对亚胺培南最敏感。27株德尔卑沙门菌呈25种PFGE带型,对复合磺胺的耐药率最高,为64.29%,对亚胺培南最敏感。结论肠炎沙门菌分子分型有明显的优势带型,本地区肠炎沙门菌对氨苄舒的耐药率最高。德尔卑沙门菌分子分型呈多样分布,复方磺胺是本地区德尔脾沙门菌最耐药抗生素。2种沙门菌都对亚胺培南最敏感。
文摘目的 对一起食用凉拌菜引起的食物中毒事件进行流行病学调查、病原学检测、溯源分析和药物敏感试验,查找食物中毒事件原因,及时采取有效措施控制污染源。方法 采集病例粪便样本22份,食品样本30份,对采集样本进行分离鉴定、脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)、药敏试验。结果 采集的52份样本检测出肠炎沙门菌6株,1株在采集的食品样本芹菜拌花生和腐竹检出,5株在采集的病例粪便标本检出,经PFGE分子分型分析,6株肠炎沙门菌带型相似度均在95.3%以上,为高度同源,耐药结果显示6菌株耐药普一致。结论 此次食物中毒事件是食用被肠炎沙门菌污染的芹菜拌花生和腐竹引起,通过实验室细菌分离鉴定、PFGE对病原菌追溯,快速有效的处置食物中毒事件。
