The Effect of Soil Enzymes and Polysaccharides Secreted by the Roots of Salvia miltiorrhiza Bunge under Drought,High Temperature,and Nitrogen and Phosphorus Deficits

Root exudates serve as crucial mediators for information exchange between plants and soil,and are an important evolutionary mechanism for plants’adaptation to environmental changes.In this study,15 different abiotic stress models were established using various stress factors,including drought(D),high temperature(T),nitrogen deficiency(N),phosphorus deficiency(P),and their combinations.We investigated their effects on the seedling growth of Salvia miltiorrhiza Bunge and the activities of Solid-Urease(S-UE),Solid-Nitrite Reductase(S-NiR),Solid-Nitrate Reductase(S-NR),Solid-Phosphotransferase(S-PT),and Solid-Catalase(S-CAT),as well as the contents of polysaccharides in the culture medium.The results showed that the growth of S.miltiorrhiza was inhibited under 15 stress conditions.Among them,13 stress conditions increased the root-shoot ratio.These 15 stress conditions significantly reduced the activity of S-NR,two combinations significantly improved the activity of S-NIR,they were synergistic stresses of high temperature and nitrogen deficiency(TN),and synergistic stresses of drought and nitrogen deficiency(DN)(p<0.05).The activity of S-UE was significantly improved under N,D,T,synergistic stresses of drought and high temperature(DT),DN,synergistic stresses of drought and phosphorus deficiency(DP),and synergistic stresses of high temperature,nitrogen,and phosphorus deficiency(TNP)stress conditions(p<0.05).Most stress combinations reduced the activity of S-PT,but D and T significantly improved it.(p<0.05).The N,DN,and TN stress conditions significantly reduced S-CAT activity.The P,DT,and synergistic stresses of drought,high temperature,and phosphorus deficiency(DTP)significantly decreased the total polysaccharide content of the soil(p<0.05).The research suggested that abiotic stress hindered the growth of S.miltiorrhiza and altered the behavior of root secretion.Roots regulated the secretion of several substances in response to various abiotic stresses,including soil nitrogen cycle enzymes,phosphorus transport-related enzymes,and antioxidant enzymes.In conclusion,plants regulate the utilization of rhizosphere substances in response to abiotic stresses by modulating the exudation of soil enzymes and polysaccharides by the root system.At the same time,soil carbon sequestration was affected by the adverse environment,which restricted the input of organic matter into the soil.

Hypoglycemic Effect of Radix Et Rhizoma Salviae Miltiorrhizae,Guixi Green Tea and Their Compatibility on Mice

[Objectives]To explore the hypoglycemic effect of Radix Et Rhizoma Salviae Miltiorrhizae,Guixi green tea and their compatibility on mice,so as to provide more options for the adjuvant treatment of diabetes and improve the comprehensive utilization value of Guixi green tea.[Methods]After being fed adaptively for 7 d,60 SPF-grade male mice were intraperitoneally injected with alloxouracil(180 mg/kg)to induce hyperglycemia model,from which 35 mice with a successful model were selected.They were randomly divided into 5 groups(Danshen group,green tea group,compatible group,positive control group and blank control group),with 7 animals in each group.The mice in all groups were administered according to the dose of 200 mg/kg once a day for 15 d.After the last administration,their body weight was measured on the 15 th day after fasting for 12 h,and their fasting blood glucose was measured by tail clipping.[Results]After modeling,the mice in each group showed typical hyperglycemia symptoms,namely obvious polydipsia,polyphagia,polyuria and weight loss.Compared with the blank control group,Danshen group and green tea group had obvious hypoglycemic effect(P<0.05),and compatible group had significant hypoglycemic effect(P<0.01).Compared with the blank control group,the weight loss of diabetic mice could also be inhibited in green tea group and compatible group(P<0.05).[Conclusions]Danshen,Guixi green tea and their compatibility had the effect of lowering blood sugar,among which the compatible group had the most obvious effect.

Systematic investigation of Radix Salviae for treating diabetic peripheral neuropathy disease based on network Pharmacology

BACKGROUND Diabetic peripheral neuropathy(DPN)is a debilitating complication of diabetes mellitus with limited available treatment options.Radix Salviae,a traditional Chinese herb,has shown promise in treating DPN,but its therapeutic mech-anisms have not been systematically investigated.AIM Radix Salviae(Danshen in pinin),a traditional Chinese medicine(TCM),is widely used to treat DPN in China.However,the mechanism through which Radix Salviae treats DPN remains unclear.Therefore,we aimed to explore the mechanism of action of Radix Salviae against DPN using network pharmacology.METHODS The active ingredients and target genes of Radix Salviae were screened using the TCM pharmacology database and analysis platform.The genes associated with DPN were obtained from the Gene Cards and OMIM databases,a drug-com-position-target-disease network was constructed,and a protein–protein inter-action network was subsequently constructed to screen the main targets.Gene Ontology(GO)functional annotation and pathway enrichment analysis were performed via the Kyoto Encyclopedia of Genes and Genomes(KEGG)using Bioconductor.RESULTS A total of 56 effective components,108 targets and 4581 DPN-related target genes of Radix Salviae were screened.Intervention with Radix Salviae for DPN mainly involved 81 target genes.The top 30 major targets were selected for enrichment analysis of GO and KEGG pathways.CONCLUSION These results suggested that Radix Salviae could treat DPN by regulating the AGE-RAGE signaling pathway and the PI3K-Akt signaling pathway.Therefore,Danshen may affect DPN by regulating inflammation and apoptosis.

Salvia Polyphenolates Combined with Doxophylline in the Treatment of Patients with Chronic Pulmonary Heart Disease in the Compensated Stage

Objective:To statistically determine the effect of salvia polyphenolates combined with doxophylline treatment in patients with chronic pulmonary heart disease in the compensated stage.Methods:From January 2023 to January 2024,76 patients with chronic pulmonary heart disease in the compensated stage were selected as research subjects.The patients were divided into a research group and a reference group using a randomized numerical table method.The research group was treated with salvia polyphenolates combined with doxophylline,while the reference group received conventional therapy.The treatment effects of the two groups were compared.Results:The patients in the research group,treated with salvia polyphenolates combined with doxophylline,showed maximal ventilation of 73.26±4.83 L/min,left ventricular ejection fraction of 56.14±1.98%,and total effective treatment rate of 94.74%.These results were better than those of the reference group.The differences between the data of the research group and the reference group were statistically significant(P<0.05).Conclusion:For patients with chronic pulmonary heart disease in the compensated stage,treatment with salvia polyphenolates combined with doxophylline significantly improves maximum ventilation and left ventricular ejection fraction,and also results in a higher total effective treatment rate.

Study on Optimization of Ethanol Reflux Extraction of Phenolic Acids from Salvia miltiorrhiza

The extraction technology of phenolic acid compounds from Salvia miltiorrhiza by ethanol reflux was studied. In this experiment, salvianolic acid B standard was used to make the standard curve. Single factor experiment and orthogonal experiment were used to study the extraction of different ethanol concentrations, reflux times and material-to-liquid ratios. The OD value of salvianolic acid compounds was measured with a spectrophotometer. The extraction rate of phenolic acid compounds under different extraction conditions was calculated through a regression equation, so as to obtain the optimal conditions for the ethanol reflux extraction process of Salvia miltiorrhiza. The experimental data can provide a reference for the ethanol reflux extraction process of salvianolic acids in the industry. According to the experiment, the extraction rate of phenolic acids in Salvia miltiorrhiza was the highest when the ethanol concentration was 60%, the reflux time was 1.5 hours, and the ratio of material-to-liquid was 1:10.

Plant growth-promoting properties and anti-fungal activity of endophytic bacterial strains isolated from Thymus altaicus and Salvia deserta in arid lands

Endophytes,as crucial components of plant microbial communities,significantly contribute to enhancing the absorption of nutrients such as nitrogen and phosphorus by their hosts,promote plant growth,and degrade pathogenic fungal mycelia.In this study,an experiment was conducted in August 2022 to explore the growth-promoting potential of endophytic bacterial strains isolated from two medical plant species,Thymus altaicus and Salvia deserta,using a series of screening media.Plant samples of Thymus altaicus and Salvia deserta were collected from Zhaosu County and Habahe County in Xinjiang Uygur Autonomous Region,China,in July 2021.Additionally,the inhibitory effects of endophytic bacterial strains on the four pathogenic fungi(Fusarium oxysporum,Fulvia fulva,Alternaria solani,and Valsa mali)were determined through the plate confrontation method.A total of 80 endophytic bacterial strains were isolated from Thymus altaicus,while a total of 60 endophytic bacterial strains were isolated from Salvia deserta.The endophytic bacterial strains from both Thymus altaicus and Salvia deserta exhibited plant growth-promoting properties.Specifically,the strains of Bacillus sp.TR002,Bacillus sp.TR005,Microbacterium sp.TSB5,and Rhodococcus sp.TR013 demonstrated strong cellulase-producing activity,siderophore-producing activity,phosphate solubilization activity,and nitrogen-fixing activity,respectively.Out of 140 endophytic bacterial strains isolated from Thymus altaicus and Salvia deserta,104 strains displayed anti-fungal activity against Fulvia fulva,Alternaria solani,Fusarium oxysporum,and Valsa mali.Furthermore,the strains of Bacillus sp.TR005,Bacillus sp.TS003,and Bacillus sp.TSB7 exhibited robust inhibition rates against all the four pathogenic fungi.In conclusion,the endophytic bacterial strains from Thymus altaicus and Salvia deserta possess both plant growth-promoting and anti-fungal properties,making them promising candidates for future development as growth-promoting agents and biocontrol tools for plant diseases.

Biotechnology ofα-linolenic acid in oilseed rape(Brassica napus)using FAD2 and FAD3 from chia(Salvia hispanica)

α-Linolenic acid(ALA,18:3Δ9,12,15)is an essential fatty acid for humans since it is the precursor for the biosynthesis of omega-3 long-chain polyunsaturated fatty acids(LC-PUFA).Modern people generally suffer from deficiency of ALA because most staple food oils are low or lack ALA content.Biotechnological enrichment of ALA in staple oil crops is a promising strategy.Chia(Salvia hispanica)has the highest ALA content in its seed oil among known oil crops.In this study,the FAD2 and FAD3 genes from chia were engineered into a staple oil crop,oilseed rape(Brassica napus),via Agrobaterium tumefaciens-mediated transformation of their LP4-2A fusion gene construct driven by the seed-specific promoter P_(NapA).In seeds of T0,T1,and T2 lines,the average ALA contents were 20.86,23.54,and 24.92%,respectively,which were 2.21,2.68,and 3.03 folds of the non-transformed controls(9.42,8.78,and 8.22%),respectively.The highest seed ALA levels of T0,T1,and T2 plants were 38.41,35.98,and 39.19%respectively,which were 4.10-4.77 folds of the respective controls.FA-pathway enzyme genes(BnACCD,BnFATA,BnSAD,BnSCD,BnDGAT1,BnDGAT2,and BnDGAT3)and positive regulatory genes(BnWRI1,BnLEC1,BnL1L,BnLEC2,BnABI3,BnbZIP67,and BnMYB96)were all significantly up-regulated.In contrast,BnTT1,BnTT2,BnTT8,BnTT16,BnTTG1,and BnTTG2,encoding negative oil accumulation regulators but positive secondary metabolism regulators,were all significantly down-regulated.This means the foreign ShFAD2-ShFAD3 fusion gene,directly and indirectly,remodeled both positive and negative loci of the whole FA-related network in transgenic B.napus seeds.

Chemical constituents and pharmacological activities of Salvia miltiorrhiza

Salvia miltiorrhiza Bunge(S.miltiorrhiza),a perennial plant of the genus Salvia,is widely used in traditional folklore medicine.Previous chemical research on this plant contains diterpenoid quinones,phenolic acids,polysaccharides and other compounds.The pharmacological investigation of S.miltiorrhiza has shown that it has various pharmacological activities,such as cardiovascular system protection,anti-inflammatory,anti-oxidant,anti-tumor,liver protection,and neuroprotection activities.This research tends to give an overview of the main chemical constituents and pharmacological effects of S.miltiorrhiza,aiming to reveal its potential value and provide reference for its further development.

不同垄作方式和种植密度对丹参产量与主要成分含量的影响

为探究不同栽培方式和生长时期对丹参根与地上部产量和主要活性成分含量的影响,本研究以紫花丹参为试验材料,采用小垄单行[三种密度:H1(8万株·hm^(-2))、H2(10万株·hm^(-2))、H3(13.3万株·hm^(-2))]与大垄双行[三种密度:H4(8万株·hm^(-2))、H5(10万株·hm^(-2))、H6(13.3万株·hm^(-2))]两种垄作方式,动态取样,测定并分析不同垄作方式、种植密度和生长时期的单株重、产量、主要活性成分含量及积累规律。结果表明,大垄双行丹参单株干重、产量、根的主要活性成分含量平均值均高于小垄单行。根的单株干重和产量的最佳处理分别为H5(48.09 g)、H4(5986.69 kg·hm^(-2)),均在11月19日最高;地上部单株干重和产量的最佳处理均为H4(50.34 g、6712.75 kg·hm^(-2)),均在10月14日最高。根中主要活性成分总含量(总丹参酮+总黄酮+总酚酸)在两种垄作方式下均呈“升-降-升”的变化趋势,最佳处理为H4(295.4 mg·g^(-1)),在9月7日最高;叶、茎中(总黄酮+总酚酸)总含量的最佳处理分别为H4(238.42 mg·g^(-1))、H6(122.43 mg·g^(-1)),均在10月14日最高;丹参酮ⅡA、丹参酮I、隐丹参酮和丹酚酸B四种活性成分总含量的最佳处理为H6(63.23 mg·g^(-1))。综上,大垄双行栽培方式优于小垄单行。本研究结果为丹参的合理种植及根与地上部综合开发利用提供了理论依据。

鼠尾草属植物资源的分类研究进展

鼠尾草属(Salvia L.)全球约有1000个种,国外许多种已在药用、观赏、食用、精油和保健等诸多方面开发其价值。我国已发现84个种24个变种,资源丰富,但广泛栽培和应用的种质仅有供以药用的丹参(S.miltiorrhiza Bunge)。为促进该属植物在我国的高效开发和推广,本文在经典植物分类学基础上,依托本课题组10余年野外调查与栽培观测的扎实数据,重点针对鼠尾草的园艺学特性进行归纳分类与特点描述,包含用途、花期、花色、花大小、茎特征以及园林造型等6个方面。根据花期可分为春花(4-6月)、夏花(7-8月)和秋花类型(9-11月),花色包含紫色、蓝色、红色、粉色、黄色和白色系列。按照花朵大小分为小型(<1.5 cm)、中型(1.5~3.0 cm)、中大型(3.0~4.5 cm)和大型花种类(>4.5 cm)。株型主要为蔓生、丛生和直立类型,部分种质在园林造型方面的可塑性强。国内鼠尾草资源以春季花期、紫色和蓝色以及中(大)型花朵的丛生草本类型为主。本研究完善了鼠尾草属植物的应用分类体系,为我国鼠尾草属资源的深度开发和广泛应用提供参考。

基于网络药理学和分子对接探讨丹参治疗冠心病和脑卒中的“异病同治”作用机制研究

目的:基于网络药理学及分子对接方法探讨丹参有效成分治疗冠心病和脑卒中“异病同治”的作用机制及关键靶点。方法:通过中药系统药理学数据库和分析平台(TCMSP)、在线人类孟德尔遗传数据库(OMIM)、DrugBank等数据库筛选丹参治疗冠心病及脑卒中的潜在靶点,并通过Metascape在线平台进行靶点蛋白-蛋白相互作用(PPI)、基因本体(GO)和京都基因与基因组百科全书(KEGG)通路分析,使用Cytoscape 3.9.1构建活性成分-关键靶点-核心通路网络,筛选出核心成分。采用分子对接模拟核心成分与关键靶点的结合程度。结果:筛选丹参治疗冠心病与脑卒中“异病同治”的潜在靶点52个,主要富集于糖基化终末产物/糖基化终末产物受体(AGE/RAGE)、血小板激活、脂质与动脉粥样硬化、流体剪应力与动脉粥样硬化等通路。核心药效成分与磷脂酰肌醇-3-激酶(PI3K)、蛋白激酶B(AKT)、一氧化氮合酶(NOS3,eNOS)、B淋巴细胞瘤相关蛋白-2(Bcl-2)、环氧合酶2(PTGS2)、整合素亚基α2b(ITGA2B)、基质金属蛋白酶9(MMP9)等靶点结合稳定。结论:丹参“异病同治”冠心病与脑卒中主要通过抗血小板活化与聚集、保护心脑血管细胞、稳定斑块、减轻炎症等机制改善动脉粥样硬化,并存在靶点竞争及协同疗效的潜在可能。

荔枝草中乙酸乙酯层化学成分分析

目的:对荔枝草的乙酸乙酯层进行化学成分研究。方法:采用硅胶柱色谱、中压快速色谱和Sephadex-LH凝胶色谱等方法进行分离纯化,运用波谱手段、理化性质和文献对照进行各单体化合物的结构鉴定。结果:从荔枝草的乙酸乙酯层分离出2个单体化合物,分别鉴定为泽兰黄酮和高车前素。结论:采用中压色谱能从荔枝草乙酸乙酯萃取层中快速大量提取泽兰黄酮和高车前素,该方法省时、简便、高效,值得推广应用。

7576张含丹参-当归药对的门诊中药饮片处方回顾性分析

目的:了解江苏省中西医结合医院南部院区/南京市溧水区中医院(以下简称“该院”)含丹参-当归药对的门诊中药饮片处方情况,探究该药对的临床配伍应用规律,为该药对的合理使用提供参考。方法:采用回顾性分析方法,统计2020—2022年该院使用丹参-当归药对的7576张门诊中药饮片处方,对患者的性别、年龄、处方药味数、金额、疗程、涉及科室及病症、使用剂量、常用的配伍比例及配伍饮片等相关内容进行统计分析。结果:7576张含丹参-当归药对的门诊中药饮片处方中,女性患者处方数约为男性患者处方数的3.21倍(5776张vs.1800张),>30~40岁患者居多。单张处方的中药饮片味数多集中在16~20味,开具的疗程均<30 d。丹参的使用剂量为《中华人民共和国药典》规定的10~15 g的处方有6490张,占85.67%;当归的使用剂量为《中华人民共和国药典》规定的6~12 g的处方有6608张,占87.22%。处方数排序居前3位的科室依次为国医堂、妇科及脑病科,治疗的病证以冲任失调、心脾两虚及气滞血瘀证为主。单张处方中丹参用量大于等于当归用量,丹参与当归的配伍比例以1∶1为多,高频配伍饮片为茯苓、川芎、炒白芍。结论:基于处方用药分析,该院含丹参-当归药对的门诊中药饮片处方基本合理,为丹参-当归药对的使用剂量范围及配伍规律研究提供了数据支持,有利于促进临床合理用药。

丹参-当归治疗缺血性脑卒中作用机制网络药理学研究

目的探讨丹参-当归治疗缺血性脑卒中(CIS)的潜在作用机制。方法通过中药系统药理学数据库与分析平台(TCMSP)、Swiss TargetPrediction数据库获取丹参和当归的活性成分及作用靶点,并通过检索PubMed、中国知网相关文献进行补充;通过GeneCards、人类孟德尔遗传数据库(OMIM)、DisGeNET数据库获取CIS的潜在靶点;将丹参-当归治疗CIS的共有靶点导入String 11.0平台,构建活性成分与疾病靶点蛋白的蛋白相互作用(PPI)网络,利用Cytoscape 3.8.2软件构建疾病-药物-活性成分-靶点可视化网络;将共有靶点导入DAVID数据库进行基因本体论(GO)功能富集和京都基因与基因组百科全书(KEGG)通路富集分析,并利用Cytoscape 3.8.2软件构建活性成分-核心靶点-通路网络;利用AutoDock软件对疾病-药物-活性成分-靶点网络中度值排名前6的核心靶点和活性成分进行分子对接验证。结果共检索到82种活性成分,其中丹参65个、当归17个,潜在作用靶点787个,疾病靶点671个,共有靶点76个。度值排名前6的活性成分为丹参醇B、丹参新醌D、木犀草素、阿魏酸、藁本内酯、丹参酮ⅡA,核心靶点为MAPK14,MAPK1,AKT1,PTGS2,EGFR,JAK2。共获得GO功能条目2545个,其中生物学过程2244个,细胞组成128个,分子功能173个,分别涉及对脂多糖的反应、膜筏、内肽酶活性等;KEGG信号通路152条,主要涉及PI3K-Akt信号通路、脂质和动脉粥样硬化、钙信号通路等。分子对接结果证明了6种活性成分与其相应核心靶点均有较好的结合活性。结论丹参-当归治疗CIS具有多成分-多靶点-多通路的调控特点,其作用机制可能与抗炎、抗氧化应激、抗神经细胞凋亡、调节自噬功能等有关。

胆汁酸诱导胃癌的发生机制及丹参提取物潜在作用的研究进展

胆汁反流可导致胃黏膜发生炎性反应、癌变。胆汁酸可以通过激活各种受体和信号通路来诱导胃癌的发生。传统中药丹参提取物可以起到预防胆汁酸诱导胃癌的作用。文章对近年来胆汁酸在胃癌发生中的作用机制进行分析,胃黏膜长时间暴露于胆汁酸中会导致黏膜损伤及炎性反应,导致慢性胃炎、肠化生、异型增生和胃癌;丹参提取物在预防和治疗胆汁酸诱导的胃癌中具有潜在优势,丹参素通过调节多种涉及炎症、氧化应激、DNA损伤和细胞凋亡的信号通路,可以预防胆汁反流引起胃炎和胃癌。

甘西鼠尾草化学成分和药理作用研究进展及质量标志物预测分析

甘西鼠尾草是一味药用价值较高的中药材,为丹参替代品广泛应用于临床。其含有萜类、酚酸类、挥发油、氨基酸和苷类等化学成分,具有抗炎、抑菌、抗氧化、抗心脑血管疾病等药理作用。通过查阅国内外相关文献,对甘西鼠尾草的化学成分和药理作用进行归纳总结,并对其质量标志物进行预测,以期为甘西鼠尾草进一步深入开发研究和临床应用提供参考。

丹参对糖尿病肾病保护作用及机制研究

糖尿病肾病(diabetes nephropathy,DN)是导致终末期肾脏病的首位致病因素,严重威胁患者生命健康。传统中医药在糖尿病肾病的治疗中具有重要潜力作用,丹参作为活血化瘀药代表,具有活血祛瘀、清心除烦之功。经研究发现,丹参含有丹酚酸、丹参酮、挥发油、多糖、含氮类化合物等多种成分,能够通过多途径多靶点改善肾小球超滤过状态和间质纤维化等病理改变,其中丹酚酸类和丹参酮类是丹参发挥糖尿病肾病保护作用的主要生物活性成分,具有降糖、调脂、抗炎、抗氧化应激、改善血流动力学、纠正肠道菌群紊乱、调节自噬水平等多种药理作用。该文参考近年来丹参相关制剂干预糖尿病肾病的临床研究和动物实验,对丹参在糖尿病肾病发生发展过程中起到的作用机制进行归纳总结,以期为临床应用丹参治疗糖尿病肾病提供更好的治疗策略和理论依据。

高光谱结合人工神经网络鉴别不同来源的丹参饮片

提出了高光谱结合人工神经网络法(ANN)鉴别不同来源丹参饮片的方法。采集了9种不同来源丹参饮片的高光谱;分别采用最大最小归一化、均值中心化、标准正态变量变换、Savitzky-Golay平滑滤波、多元散射校正等5种光谱预处理方法,结合ANN建立了鉴别这些样品来源的分类模型。测试集验证结果表明,当隐含层节点数设置为17时,对光谱进行均值中心化预处理可建立最佳的ANN模型,分类准确率为98.77%。7种丹参样品判别结果的真正率、命中率和特异度均达到100.00%;其余2种丹参样品的真正率、命中率和特异度也不小于90.00%。

丹参常见病害及用药情况调查

为提高丹参病害防治技术同时为丹参生产提供基础数据,通过对济南、临沂等山东丹参主要生产区域的多个种植基地及农资经营单位走访调查,结合文献调研,对丹参常见病害、发病规律、用药种类、农残现状进行总结归纳。结果表明丹参常见病害有10种,使用包括生物农药及复合制剂等在内的20类43种农药,用药品种均符合用药规定,但存在缺乏农药登记及防治技术指导、生物防治推广不足等问题。该结果为科学防治丹参病害,促进丹参产业绿色持续发展提供依据。

施肥对鼠尾草属2种外来植物资源分配的影响

【目的】比较鼠尾草属2种外来植物一串红和椴叶鼠尾草在不同营养条件下生长、繁殖、防御方面资源分配策略,了解椴叶鼠尾草的入侵机制。【方法】采用盆栽试验法,以2种外来植物为材料,设置对照(0 g)、低肥(2 g)和高肥(4 g)3个营养梯度,测定生长、繁殖指标,物理防御、化学防御指标和营养物质含量。【结果】(1)2种植物的生物量、营养物质含量和花的化学防御能力均为高肥处理>低肥处理>对照,叶的物理和化学防御能力为高肥处理<低肥处理<对照。(2)在相同条件下,椴叶鼠尾草的株高、叶片数、花数、总生物量高于一串红,将43%~52%的总生物量分配给有性生殖,叶的物理和化学防御指标也高于一串红,但花的化学防御指标和营养物质含量比一串红低。椴叶鼠尾草叶的化学防御物质含量比花高,一串红则相反。【结论】2种植物在资源充足的条件下会减少叶防御投入,增加生长繁殖的投入。椴叶鼠尾草侧重叶的防御,一串红侧重花的防御。