Based on single-factor experiment and L9 （34 ） orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The resuhs indicated that the optimal concentration...Based on single-factor experiment and L9 （34 ） orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The resuhs indicated that the optimal concentrations of various components in a 20 ill ISSR-PCR reaction system of S. przewalskii Maxim. were ： 2 ul of 10 x buffer （ Mg2＋）, 0.4 mmol/L dNTPs, 1.0 U of Taq DNA polymerase, 0.3 umol/L ISSR primers and 30 ng of DNA template. This study laid the foun- dation for the utilization of S. przewalskii Maxim. germplasm resources.展开更多
From the root of Salvia przewalskii Maxim.a new phenolic acid, przewalskinic acid A was isolated and the structure was established by the analysis of^(13)C-NMR,~1H-NMR and two-dimensional COSY experiments.
Neo-przewaquinone A was isolated from the root of Salvia przewalskii Maxim. The structure elucidation and 1H, 13C NMR assignments were achieved by spectroscopic method.
Two new ionone derivatives, rhododendrone A and rhododendronside, have been isolated from the alcoholic extract of the aerial parts of Rhododendron Przewalskii Maxim . The structures of the two compounds, (3R,6S)...Two new ionone derivatives, rhododendrone A and rhododendronside, have been isolated from the alcoholic extract of the aerial parts of Rhododendron Przewalskii Maxim . The structures of the two compounds, (3R,6S) -3-hydroxy- α -ionone 1 and 1,1,5-trimethyl-6-(3-hydroxyl)cyclohexene-5-yl-1- β-D -pyranoglucoside 2, have been elucidated on the basis of the spectroscopic analyses.展开更多
Two new ionone derivatives, named rhododendrone and rhododendronside, were isolated from the alcoholic extract of the aerial parts of Rhododendron przwalskii Maxim. Their structures were elucidated on the basis of spe...Two new ionone derivatives, named rhododendrone and rhododendronside, were isolated from the alcoholic extract of the aerial parts of Rhododendron przwalskii Maxim. Their structures were elucidated on the basis of spectroscopic analysis展开更多
The antibacierial(staphylococcus aureus)activities of 23 diterpene quinones from the Salvia przewalskii Maxim,the artificial synthesis ones and that of the metabolism in pigs were examined in an effort to study the qu...The antibacierial(staphylococcus aureus)activities of 23 diterpene quinones from the Salvia przewalskii Maxim,the artificial synthesis ones and that of the metabolism in pigs were examined in an effort to study the quantitative structure activity relationship with the Free-Wilson method.With the application of the multipiy regression,the Free-Wilson pattern was established by the dummy(indicator)variable of the structural fragments or substituents.The results showed:(1)The diketone moiety played the fundamental role in antibacterial activities,ortho-quinones was higher active than para-quinones.(2)The single bond existed between the C15 and the C16 at D-ring led to higher activity.(3)The saturation at the A-ring led to higher activity.(4)The components adjacent to nitrogen substituents at C15 led to higher activity.(5)The hydroxylation or dehydrogenation at A-ring led to less activity.The research results can provide the theoretical basis for developing a new antibacterial drug from the Salvia diterpene quinones.展开更多
基金Supported by Project of Qinghai Science and Technology Department(2011-NF19)Fund for Middle-aged and Young Scientists of Qinghai University(2012-QYT-1)
文摘Based on single-factor experiment and L9 （34 ） orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The resuhs indicated that the optimal concentrations of various components in a 20 ill ISSR-PCR reaction system of S. przewalskii Maxim. were ： 2 ul of 10 x buffer （ Mg2＋）, 0.4 mmol/L dNTPs, 1.0 U of Taq DNA polymerase, 0.3 umol/L ISSR primers and 30 ng of DNA template. This study laid the foun- dation for the utilization of S. przewalskii Maxim. germplasm resources.
文摘From the root of Salvia przewalskii Maxim.a new phenolic acid, przewalskinic acid A was isolated and the structure was established by the analysis of^(13)C-NMR,~1H-NMR and two-dimensional COSY experiments.
文摘Neo-przewaquinone A was isolated from the root of Salvia przewalskii Maxim. The structure elucidation and 1H, 13C NMR assignments were achieved by spectroscopic method.
基金Supported by the National Natural Science Foundation of China( No.2 0 2 75 0 2 0 ) and the Natural Science Foundation ofShandong Province( No.Z2 0 0 2 B0 2 )
文摘Two new ionone derivatives, rhododendrone A and rhododendronside, have been isolated from the alcoholic extract of the aerial parts of Rhododendron Przewalskii Maxim . The structures of the two compounds, (3R,6S) -3-hydroxy- α -ionone 1 and 1,1,5-trimethyl-6-(3-hydroxyl)cyclohexene-5-yl-1- β-D -pyranoglucoside 2, have been elucidated on the basis of the spectroscopic analyses.
文摘Two new ionone derivatives, named rhododendrone and rhododendronside, were isolated from the alcoholic extract of the aerial parts of Rhododendron przwalskii Maxim. Their structures were elucidated on the basis of spectroscopic analysis
基金This work was supported by a grant from the National Tackle Key Projects of Chinathe project of sciences and techniques of CAAS.
文摘The antibacierial(staphylococcus aureus)activities of 23 diterpene quinones from the Salvia przewalskii Maxim,the artificial synthesis ones and that of the metabolism in pigs were examined in an effort to study the quantitative structure activity relationship with the Free-Wilson method.With the application of the multipiy regression,the Free-Wilson pattern was established by the dummy(indicator)variable of the structural fragments or substituents.The results showed:(1)The diketone moiety played the fundamental role in antibacterial activities,ortho-quinones was higher active than para-quinones.(2)The single bond existed between the C15 and the C16 at D-ring led to higher activity.(3)The saturation at the A-ring led to higher activity.(4)The components adjacent to nitrogen substituents at C15 led to higher activity.(5)The hydroxylation or dehydrogenation at A-ring led to less activity.The research results can provide the theoretical basis for developing a new antibacterial drug from the Salvia diterpene quinones.
