A new alkaloid has been isolated from the root of Patrinia scabra. Its structure was elucidated as 2'-acetamido-3'-phenyl propyl 2-benzamido-3-phenyl propionate by extensive spectroscopic analysis.
Objective:To exploit- the effects of gentiana scabra bage on the expression of hepatic collagen proteins in Paragonimus skrjabini rats with liver fibrosis.Methods:Immunohistochemical technique was used to observe the ...Objective:To exploit- the effects of gentiana scabra bage on the expression of hepatic collagen proteins in Paragonimus skrjabini rats with liver fibrosis.Methods:Immunohistochemical technique was used to observe the changes of content of hepatic type Ⅰ,Ⅲ collagen proteins in Paragonimus skrjabini rats with Liver fibrosis before and after the gentiana scabra bage treatmeat.Results:Comparing with the model group,changes of hepatic tvpe Ⅰ and type Ⅲ collagen proteins in gentiana scabra bage treated group were significantly weakened.Conclusions:Gentiana scabra bage treatment can reduce the content of hepatic type Ⅲ and type Ⅰ collagen protein significantly in Paragonimus skrjabini rats with liver fibrosis,thereby,playing the role against hepatic fibrosis.展开更多
The nutritional values of different species of sea cucumber are greatly concerned because of their dietary and curative properties. In this study, two species of sea cucumber, Holothuria edulis, a low-valued noncommer...The nutritional values of different species of sea cucumber are greatly concerned because of their dietary and curative properties. In this study, two species of sea cucumber, Holothuria edulis, a low-valued noncommercial species, and Holothuria scabra, a high-valued commercial species were selected to compare its proximate composition and fatty acids. H. edulis a prevalent species in coastal water of Sabah is not commercially importance like H. scabra. Sea cucumbers were captured live from the Sabah marine habitat. All samples were immediately eviscerated, freeze-dried and stored at 4°C in until analyzed. Silylating agent N, O-Bis(trimethylsilyl) trifluoroacetamide (BSTFA) was used to derivitization of fatty acid prior to gas chromatography-mass spectrometry (GC-MS) analysis. Proximate compositions (%), such as moisture, crude protein, crude lipids and ash were carried using standard methods. Major fatty acids in H. edulis and H. scabra were saturated fatty acid (SFA) accounted for 83.95% and 98.60%, respectively and dominated with Palmitic acids. Polyunsaturated fatty acid (PUFA), arachidonic acid of 16.05% was found only in H. edulis, but absent H. scabra. Proximate compositions (dry weight) were varied greatly within these two species. Moisture, crude protein, crude lipids and ash of 85.5%, 70.5%, 1.37% and 1.27% respectively were obtained in H. edulis. On the other hand 84.5% of moisture, 51.2% of crude protein, 0.27% of crude lipids and 4.44% of ashes were determined in H. scabra. Significantly higher protein (p H. edulis compare to H. scabra could be the choice of option for the utilization of this non commercial species as nutraceutical industry and also alternatives to reduce the pressure on heavily exploited species of H. scabra.展开更多
Antimicrobial use in livestock is faced with various challenges including emergence of antimicrobial resistance and presence of drug residues in meat products,hence the need for alternatives.The aim of this work was t...Antimicrobial use in livestock is faced with various challenges including emergence of antimicrobial resistance and presence of drug residues in meat products,hence the need for alternatives.The aim of this work was to assess the effect a plant(Zehneria scabra)extract on Salmonella infected quails,as an alternative to antibiotic therapy.Quails were randomly assigned into six groups each containing twelve birds.The neutral control(T0)group was not infected and received tap water whereas other groups were infected.The negative control(T-)received tap water.The positive control(T+)received a single dose of oxytetracycline(20 mg/kg).T1,T2 andT3 orally received the plant extract at the following respective doses:9,18 and 37 mg/kg.Quails were infected by oral administration of a single dose of Salmonella Enteritidis(10^(5)CFU).Haematological and biochemical parameters were evaluated.From day 2 to day 9 to day 16,the bacterial load of all treatment groups(T+,T1,T2,T3)decreased.The infection resulted in a significant(p<0.05)increase in serum levels of alanine aminotransferase,triglycerides,total cholesterol and white blood cells,and a significant decrease in the liver and kidney protein content.The treatment resulted in the correction of the aforementioned effects.The plant extract(18 mg/kg)is as effective as oxytetracycline,and can be safely used in phytomedicine for the treatment of Salmonella Enteritidis infection without kidney and liver damage.展开更多
Objective To study the new triterpene glycosides from sea cucumber Holothuria scabra with cytotoxic activity. Methods Triterpene glycosides from H. scabra were separated and purified by chromatography on DA-101, silic...Objective To study the new triterpene glycosides from sea cucumber Holothuria scabra with cytotoxic activity. Methods Triterpene glycosides from H. scabra were separated and purified by chromatography on DA-101, silica gel, and reversed-phase silica gel column, as well as RP-HPLC. Their structures were elucidated on the basis of spectral data and chemical evidence. Results Three triterpene glycosides were identified as scabraside D (1), fuscocineroside C (2), and 24-dehydroechinoside A (3). Their inhibition on P-388, A549, MKN-28, HCT116, and MCF-7 cells were significant. Conclusion Scabraside D (1) is a new triterpene glycoside, and compounds 2 and 3 are isolated from H. scabra for the first time. The glycosides 1-3 show the in vitro cytotoxicity against five human tumor cell lines in comparison to 10-hydroxycamptothecin.展开更多
AIM: To study the chemical constituents and bioactivity of the roots of Patrinia scabra Bunge. METHODS: The chemical constituents were isolated using various chromatographic methods, and the structures were elucidated...AIM: To study the chemical constituents and bioactivity of the roots of Patrinia scabra Bunge. METHODS: The chemical constituents were isolated using various chromatographic methods, and the structures were elucidated on the basis of spectral analysis and chemical methods. In addition, cytotoxic activities toward HepG2 cells were tested by the MTT method. RESULTS: A new triterpenoid saponin, 3-O-(4'-isovaleryl)-O-β-D-xylose-12,30-dihydroxy-oleanane-28,13-lactone-22-O- β-D-glucoside(1), along with two known triterpenoid saponins, acanthopanax saponin CP3(2) and foetoside C(3), were isolated. CONCLUSION: The aglycone of compound 1 was a new skeleton derivative of oleanolic acid. Compound 2 showed strong cytotoxicity to HePG2 cells(IC50 1.49 μmol?L-1).展开更多
Objective:To provide a scientific basis for identifying Richardia scabra with the help of pharmacognosy parameters,which has never been done before.Methods:Roots,stems,and leaves of R.scabra were collected for pharmac...Objective:To provide a scientific basis for identifying Richardia scabra with the help of pharmacognosy parameters,which has never been done before.Methods:Roots,stems,and leaves of R.scabra were collected for pharmacognostic studies involving source identification,character observation,microscopic evaluation,phytochemical screening,ultraviolet spectrum analysis,as well as DNA barcoding analysis.Results:The results showed that it had strong microscopic characters,so it could be used to identify R scabra.Phytochemical study showed the presence of carbohydrates,tannins,and proteins from water extract,and the data related to moisture,ash content,and molecular pharmacognosy were obtained.Conclusion:The established method in this study is easy and accurate,which provides a reference basis for its further development and utilization.展开更多
文摘A new alkaloid has been isolated from the root of Patrinia scabra. Its structure was elucidated as 2'-acetamido-3'-phenyl propyl 2-benzamido-3-phenyl propionate by extensive spectroscopic analysis.
基金supported by National Natural Science Foundation of China(No.81360252:81360128)Natural Science Foundation of Yunnan Province(No.2012FB025)
文摘Objective:To exploit- the effects of gentiana scabra bage on the expression of hepatic collagen proteins in Paragonimus skrjabini rats with liver fibrosis.Methods:Immunohistochemical technique was used to observe the changes of content of hepatic type Ⅰ,Ⅲ collagen proteins in Paragonimus skrjabini rats with Liver fibrosis before and after the gentiana scabra bage treatmeat.Results:Comparing with the model group,changes of hepatic tvpe Ⅰ and type Ⅲ collagen proteins in gentiana scabra bage treated group were significantly weakened.Conclusions:Gentiana scabra bage treatment can reduce the content of hepatic type Ⅲ and type Ⅰ collagen protein significantly in Paragonimus skrjabini rats with liver fibrosis,thereby,playing the role against hepatic fibrosis.
文摘The nutritional values of different species of sea cucumber are greatly concerned because of their dietary and curative properties. In this study, two species of sea cucumber, Holothuria edulis, a low-valued noncommercial species, and Holothuria scabra, a high-valued commercial species were selected to compare its proximate composition and fatty acids. H. edulis a prevalent species in coastal water of Sabah is not commercially importance like H. scabra. Sea cucumbers were captured live from the Sabah marine habitat. All samples were immediately eviscerated, freeze-dried and stored at 4°C in until analyzed. Silylating agent N, O-Bis(trimethylsilyl) trifluoroacetamide (BSTFA) was used to derivitization of fatty acid prior to gas chromatography-mass spectrometry (GC-MS) analysis. Proximate compositions (%), such as moisture, crude protein, crude lipids and ash were carried using standard methods. Major fatty acids in H. edulis and H. scabra were saturated fatty acid (SFA) accounted for 83.95% and 98.60%, respectively and dominated with Palmitic acids. Polyunsaturated fatty acid (PUFA), arachidonic acid of 16.05% was found only in H. edulis, but absent H. scabra. Proximate compositions (dry weight) were varied greatly within these two species. Moisture, crude protein, crude lipids and ash of 85.5%, 70.5%, 1.37% and 1.27% respectively were obtained in H. edulis. On the other hand 84.5% of moisture, 51.2% of crude protein, 0.27% of crude lipids and 4.44% of ashes were determined in H. scabra. Significantly higher protein (p H. edulis compare to H. scabra could be the choice of option for the utilization of this non commercial species as nutraceutical industry and also alternatives to reduce the pressure on heavily exploited species of H. scabra.
文摘Antimicrobial use in livestock is faced with various challenges including emergence of antimicrobial resistance and presence of drug residues in meat products,hence the need for alternatives.The aim of this work was to assess the effect a plant(Zehneria scabra)extract on Salmonella infected quails,as an alternative to antibiotic therapy.Quails were randomly assigned into six groups each containing twelve birds.The neutral control(T0)group was not infected and received tap water whereas other groups were infected.The negative control(T-)received tap water.The positive control(T+)received a single dose of oxytetracycline(20 mg/kg).T1,T2 andT3 orally received the plant extract at the following respective doses:9,18 and 37 mg/kg.Quails were infected by oral administration of a single dose of Salmonella Enteritidis(10^(5)CFU).Haematological and biochemical parameters were evaluated.From day 2 to day 9 to day 16,the bacterial load of all treatment groups(T+,T1,T2,T3)decreased.The infection resulted in a significant(p<0.05)increase in serum levels of alanine aminotransferase,triglycerides,total cholesterol and white blood cells,and a significant decrease in the liver and kidney protein content.The treatment resulted in the correction of the aforementioned effects.The plant extract(18 mg/kg)is as effective as oxytetracycline,and can be safely used in phytomedicine for the treatment of Salmonella Enteritidis infection without kidney and liver damage.
基金National High-Tech Research and Development Project (863 Project, 2006AA09Z417)National Natural Science Foundation of China (20772155)
文摘Objective To study the new triterpene glycosides from sea cucumber Holothuria scabra with cytotoxic activity. Methods Triterpene glycosides from H. scabra were separated and purified by chromatography on DA-101, silica gel, and reversed-phase silica gel column, as well as RP-HPLC. Their structures were elucidated on the basis of spectral data and chemical evidence. Results Three triterpene glycosides were identified as scabraside D (1), fuscocineroside C (2), and 24-dehydroechinoside A (3). Their inhibition on P-388, A549, MKN-28, HCT116, and MCF-7 cells were significant. Conclusion Scabraside D (1) is a new triterpene glycoside, and compounds 2 and 3 are isolated from H. scabra for the first time. The glycosides 1-3 show the in vitro cytotoxicity against five human tumor cell lines in comparison to 10-hydroxycamptothecin.
文摘AIM: To study the chemical constituents and bioactivity of the roots of Patrinia scabra Bunge. METHODS: The chemical constituents were isolated using various chromatographic methods, and the structures were elucidated on the basis of spectral analysis and chemical methods. In addition, cytotoxic activities toward HepG2 cells were tested by the MTT method. RESULTS: A new triterpenoid saponin, 3-O-(4'-isovaleryl)-O-β-D-xylose-12,30-dihydroxy-oleanane-28,13-lactone-22-O- β-D-glucoside(1), along with two known triterpenoid saponins, acanthopanax saponin CP3(2) and foetoside C(3), were isolated. CONCLUSION: The aglycone of compound 1 was a new skeleton derivative of oleanolic acid. Compound 2 showed strong cytotoxicity to HePG2 cells(IC50 1.49 μmol?L-1).
基金The authors are sincerely grateful to Guangdong Pharmaceutical University for their help in carrying out this workSupport was also provided by Guangzhou Science and Technology Plan Project(No.202002020071)We appreciate the professional assistance provided by Figdraw(www.figdraw.com)with the pattern drawing.
文摘Objective:To provide a scientific basis for identifying Richardia scabra with the help of pharmacognosy parameters,which has never been done before.Methods:Roots,stems,and leaves of R.scabra were collected for pharmacognostic studies involving source identification,character observation,microscopic evaluation,phytochemical screening,ultraviolet spectrum analysis,as well as DNA barcoding analysis.Results:The results showed that it had strong microscopic characters,so it could be used to identify R scabra.Phytochemical study showed the presence of carbohydrates,tannins,and proteins from water extract,and the data related to moisture,ash content,and molecular pharmacognosy were obtained.Conclusion:The established method in this study is easy and accurate,which provides a reference basis for its further development and utilization.
