Objective To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011. Methods Seventy-four 5treptococcol pyogenes involved in an outbreak of ...Objective To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011. Methods Seventy-four 5treptococcol pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011. Emm genotyping, pulsed-field gel electrophoresis （PFGE）, superantigen （SAg） genes and antimicrobial susceptibility profiling were analyzed for these isolates. Results A total of 4 different emm types were identified. Emm12 was the most prevalent type which contained four predominating PFGE patterns corresponding to four different virulence and superantigen profiles. Emm12（79.7%） and emml （14.9%） accounted for approximately 94% of all the isolates. The speA gene was all negative in emm12 isolates and positive in emml isolates. All strains were resistant to erythromycin, and 89.4% of them were resistant to erythromycin, tracycline, and clindamycin simultaneously. Conclusion Several highly diversified clones with a high macrolide resistance rate comprise a predominant proportion of circulating strains, though no new emm type was found in this outbreak. The data provide a baseline for further surveillance of scarlet fever, which may contribute to the explanation of the outbreak and development of a GAS vaccine in China.展开更多
This study aimed to investigate the carriage of Streptococcus pyogenes in population vulnerable to scarlet fever and to compare their genotypic characterization between different age groups. Pharyngeal swabs were coll...This study aimed to investigate the carriage of Streptococcus pyogenes in population vulnerable to scarlet fever and to compare their genotypic characterization between different age groups. Pharyngeal swabs were collected from 120 - 150 students in each of the three districts in Shanghai in May and December during 2015 to 2017, while emm typing and detection of 12 superantigen genes were performed to characterize the isolates. During 2015-2017, the average carriage rate in students was 5.7% (135/2,371), without significant difference between different years or districts. The carriage rate was significantly different between children from the three age groups, with 2.4% in 3 - 4 years, 5.4% in 5 - 9 years, and 9.1% in 10 - 14 years. Eight emm types were found, including emm 1, emm 4, emm 12, emm 22, emm 75, emm 89, emm 70 and emm 241, among which emm 12 accounted for 60%, and emm 1 27.5%. The predominance of emm 12 was found in each year, but the proportion of emm 12 was lower in 10 - 14 years (43.3%) than in 3 - 4 years (86.7%) and in 5 - 9 years (73.3%) (P = 0.002 and 0.003). Superantigen genes of speB, speC, speG, ssa and smeZ were found in almost all the isolates. The average carriage of S. pyogenes in population vulnerable to scarlet fever was 5.7% in Shanghai, highest in 10 - 14 years (9.1%), while emm 12 was the predominant type.展开更多
目的探索北京市猩红热发病的时空分布、演变特征及热点区域,为制定精准防控措施提供依据。方法采用SaTScan软件进行时间扫描和时空扫描分析。利用R软件spdep包进行全局空间自相关分析以判断有无聚集,采用局部Getis-Ord(G_( i )^(∗))进...目的探索北京市猩红热发病的时空分布、演变特征及热点区域,为制定精准防控措施提供依据。方法采用SaTScan软件进行时间扫描和时空扫描分析。利用R软件spdep包进行全局空间自相关分析以判断有无聚集,采用局部Getis-Ord(G_( i )^(∗))进行热点分析,定位发病的局部聚集区域。结果2015-2020年北京市猩红热发病率由西南到东北呈逐渐降低趋势。全局空间自相关分析显示,2015-2020年猩红热发病率存在空间聚集现象(Moran’s I=0.406,P<0.001),且为高-高聚集[G_( i )^(∗)=0.005,E(G_( i )^(∗))=0.003]。热点分析结果显示,10个区的48个乡、镇、街道为热点区域,主要分布于南部及西南部郊区,涉及区主要为大兴(12个)、房山(7个)和门头沟区(6个)。时空分析显示,2017年4月-2019年1月北京市东南方向存在一个聚集区,2017年4月-2019年12月北京西南方向存在一个聚集区域。结论2017-2019年北京市猩红热发病略有增加。丰台、房山、门头沟和大兴区等各区交界地应为北京市猩红热防控的重点地区,应在该区域重点人群中加强环境治理,并开展针对呼吸道传染病的预防措施及宣传教育。展开更多
2022年末世界卫生组织通报多国猩红热等A族链球菌(group A Streptococcus,GAS)感染增多,以10岁内儿童多见,死亡病例也超过预期,引起了国际社会的普遍关注。该文梳理此次GAS疾病暴发的现状,以及发生的原因和应对措施,希望能引起我国临床...2022年末世界卫生组织通报多国猩红热等A族链球菌(group A Streptococcus,GAS)感染增多,以10岁内儿童多见,死亡病例也超过预期,引起了国际社会的普遍关注。该文梳理此次GAS疾病暴发的现状,以及发生的原因和应对措施,希望能引起我国临床工作者的重视,提高认知和警惕性,及早发现新型冠状病毒感染(coronavirus disease 2019)管控措施优化后可能出现的感染性疾病异常的流行病学变化,以保障儿童健康。展开更多
基金supported by a fund for China Mega-Project for Infectious Disease(2011ZX10004-001)
文摘Objective To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011. Methods Seventy-four 5treptococcol pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011. Emm genotyping, pulsed-field gel electrophoresis （PFGE）, superantigen （SAg） genes and antimicrobial susceptibility profiling were analyzed for these isolates. Results A total of 4 different emm types were identified. Emm12 was the most prevalent type which contained four predominating PFGE patterns corresponding to four different virulence and superantigen profiles. Emm12（79.7%） and emml （14.9%） accounted for approximately 94% of all the isolates. The speA gene was all negative in emm12 isolates and positive in emml isolates. All strains were resistant to erythromycin, and 89.4% of them were resistant to erythromycin, tracycline, and clindamycin simultaneously. Conclusion Several highly diversified clones with a high macrolide resistance rate comprise a predominant proportion of circulating strains, though no new emm type was found in this outbreak. The data provide a baseline for further surveillance of scarlet fever, which may contribute to the explanation of the outbreak and development of a GAS vaccine in China.
文摘This study aimed to investigate the carriage of Streptococcus pyogenes in population vulnerable to scarlet fever and to compare their genotypic characterization between different age groups. Pharyngeal swabs were collected from 120 - 150 students in each of the three districts in Shanghai in May and December during 2015 to 2017, while emm typing and detection of 12 superantigen genes were performed to characterize the isolates. During 2015-2017, the average carriage rate in students was 5.7% (135/2,371), without significant difference between different years or districts. The carriage rate was significantly different between children from the three age groups, with 2.4% in 3 - 4 years, 5.4% in 5 - 9 years, and 9.1% in 10 - 14 years. Eight emm types were found, including emm 1, emm 4, emm 12, emm 22, emm 75, emm 89, emm 70 and emm 241, among which emm 12 accounted for 60%, and emm 1 27.5%. The predominance of emm 12 was found in each year, but the proportion of emm 12 was lower in 10 - 14 years (43.3%) than in 3 - 4 years (86.7%) and in 5 - 9 years (73.3%) (P = 0.002 and 0.003). Superantigen genes of speB, speC, speG, ssa and smeZ were found in almost all the isolates. The average carriage of S. pyogenes in population vulnerable to scarlet fever was 5.7% in Shanghai, highest in 10 - 14 years (9.1%), while emm 12 was the predominant type.
文摘目的探索北京市猩红热发病的时空分布、演变特征及热点区域,为制定精准防控措施提供依据。方法采用SaTScan软件进行时间扫描和时空扫描分析。利用R软件spdep包进行全局空间自相关分析以判断有无聚集,采用局部Getis-Ord(G_( i )^(∗))进行热点分析,定位发病的局部聚集区域。结果2015-2020年北京市猩红热发病率由西南到东北呈逐渐降低趋势。全局空间自相关分析显示,2015-2020年猩红热发病率存在空间聚集现象(Moran’s I=0.406,P<0.001),且为高-高聚集[G_( i )^(∗)=0.005,E(G_( i )^(∗))=0.003]。热点分析结果显示,10个区的48个乡、镇、街道为热点区域,主要分布于南部及西南部郊区,涉及区主要为大兴(12个)、房山(7个)和门头沟区(6个)。时空分析显示,2017年4月-2019年1月北京市东南方向存在一个聚集区,2017年4月-2019年12月北京西南方向存在一个聚集区域。结论2017-2019年北京市猩红热发病略有增加。丰台、房山、门头沟和大兴区等各区交界地应为北京市猩红热防控的重点地区,应在该区域重点人群中加强环境治理,并开展针对呼吸道传染病的预防措施及宣传教育。
文摘2022年末世界卫生组织通报多国猩红热等A族链球菌(group A Streptococcus,GAS)感染增多,以10岁内儿童多见,死亡病例也超过预期,引起了国际社会的普遍关注。该文梳理此次GAS疾病暴发的现状,以及发生的原因和应对措施,希望能引起我国临床工作者的重视,提高认知和警惕性,及早发现新型冠状病毒感染(coronavirus disease 2019)管控措施优化后可能出现的感染性疾病异常的流行病学变化,以保障儿童健康。
