Jasmine(Jasminum sambac Aiton)is a well-known cultivated plant species for its fragrant flowers used in the perfume industry and cosmetics.However,the genetic basis of its floral scent is largely unknown.In this study...Jasmine(Jasminum sambac Aiton)is a well-known cultivated plant species for its fragrant flowers used in the perfume industry and cosmetics.However,the genetic basis of its floral scent is largely unknown.In this study,using PacBio,Illumina,10×Genomics and highthroughput chromosome conformation capture(Hi-C)sequencing technologies,a high-quality chromosome-level reference genome for J.sambac was obtained,exploiting a double-petal phenotype cultivar‘Shuangbanmoli’(JSSB).The results showed that the final assembled genome of JSSB is 580.33 Mb in size(contig N50=1.05 Mb;scaffold N50=45.07 Mb)with a total of 39618 predicted protein-coding genes.Our analyses revealed that the JSSB genome has undergone an ancient whole-genome duplication(WGD)event at 91.68 million years ago(Mya).It was estimated that J.sambac diverged from the lineage leading to Olea europaea and Osmanthus fragrans about 28.8 Mya.On the basis of a combination of genomic,transcriptomic and metabolomic analyses,a range of floral scent volatiles and genes were identified involved in the benzenoid/phenylpropanoid and terpenoid biosynthesis pathways.The results provide new insights into the molecular mechanism of its fragrance biosynthesis in jasmine.展开更多
Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions, 5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA （RAPD） and inter-simple seq...Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions, 5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA （RAPD） and inter-simple sequence repeat （ISSR） marker systems. The varieties analyzed by 11 RAPD and 8 ISSR primers yielded an average of 65% and 80% polymorphism, respectively. The average number of polymorphic bands generated per RAPD primer was 6 and per ISSR primer was 5.87. RAPD and ISSR data analysis individually could not segregate basmati and non-basmati scented rice accessions. However, the analysis using a combined data could group basmati and non-basmati scented rice accessions separately. The bands present specifically among three accessions of non-basmati scented rice were also identified. The study revealed a high genetic diversity among non-basmati scented rice accessions.展开更多
Containing higher aromatic composition,scented rice is better than ordinary rice varieties in taste and nutrition. Major composition of scented rice is 2-acetyl-1-pyrroline( 2-AP). Different scented rice varieties hav...Containing higher aromatic composition,scented rice is better than ordinary rice varieties in taste and nutrition. Major composition of scented rice is 2-acetyl-1-pyrroline( 2-AP). Different scented rice varieties have different content of 2-AP. The content of 2-AP is not only influenced by genetic factors,but also closely connected with types and content of nutrition elements in soil,soil moisture management,environmental temperature,and storage. 2-AP synthesis and regulation are possibly connected with Proline and enzyme.展开更多
This novel rice line is developed recently’by a research team led by Associate Prof QIU Baiqin, Crop Breeding and Cultivation Institute, Zhejiang Academy of Agricultural Sciences. Zhe 9248 is of high resistance to bl...This novel rice line is developed recently’by a research team led by Associate Prof QIU Baiqin, Crop Breeding and Cultivation Institute, Zhejiang Academy of Agricultural Sciences. Zhe 9248 is of high resistance to blast, early mature and of higher yield. Being soft, smooth, and glossy, the cooked rice of "Zhe 9248" has excellent eating quality. The kernels of "Zhe 9248" are hardened to such a degree that they could be capa-展开更多
We analyzed the inheritance of fragrance bysoaking rice grains or leafblades in 17g/l KOHsolution, using scented rice (Shengxiangjing 4)and non-scented rice (Huxuan 19 and Lao-laiqing) as materials. The Fplants of var...We analyzed the inheritance of fragrance bysoaking rice grains or leafblades in 17g/l KOHsolution, using scented rice (Shengxiangjing 4)and non-scented rice (Huxuan 19 and Lao-laiqing) as materials. The Fplants of various crosses did notpresent fragrance. In the Fthe segregating ra-tio of fragrance and non-fragrance plants and展开更多
为探明室温贮藏对花果香红茶风味品质和生化组成的影响,本研究比较了4种不同年份(0、1、2、3a)花果香红茶风味感官品质,并采用顶空固相微萃取-气相色谱-质谱联用技术和超高效液相色谱-静电场轨道阱高分辨质谱对花果香红茶中挥发性化合...为探明室温贮藏对花果香红茶风味品质和生化组成的影响,本研究比较了4种不同年份(0、1、2、3a)花果香红茶风味感官品质,并采用顶空固相微萃取-气相色谱-质谱联用技术和超高效液相色谱-静电场轨道阱高分辨质谱对花果香红茶中挥发性化合物和非挥发性化合物进行检测。结果表明,室温贮藏对花果香红茶的感官品质影响显著,室温贮藏3 a茶叶失去花果香品质特征,以陈酸等品质特征为主。基于主成分分析和系统聚类分析均可将供试茶样划分成不同年份的4个类群,基于偏最小二乘判别分析和单因素方差分析可从不同贮藏年份花果香红茶中筛选出芳樟醇、顺式-β-罗勒烯、己酸等15种挥发性差异化合物(变量投影重要性(variable importance in project,VIP)>1和P<0.05),茶氨酸、表儿茶素、葡萄糖酸等154种非挥发性差异化合物(VIP>2和P<0.05)。挥发性化合物中芳樟醇、己醛、顺式-β-罗勒烯、2-戊基呋喃等的含量随贮藏年份增加而减少,而己酸、二氢猕猴桃内酯、1-乙基-2-甲酰基吡咯、反式-β-紫罗兰酮等的含量随贮藏年份增加而增加。非挥发性化合物中大部分氨基酸类、核苷酸类、糖类、茶多酚等的含量随贮藏时间延长而减少,而有机酸和脂类化合物等的含量多呈增加趋势。研究结果可为阐明花果香红茶贮藏过程的风味品质变化提供科学依据,也有利于引导市场理性存茶和科学消费。展开更多
The scent of receptive females as a signal to reproduction stimulates male mice to olfactory search of a potential breeding partner.This searching behavior is coupled with infection risk due to bacterial contamination...The scent of receptive females as a signal to reproduction stimulates male mice to olfactory search of a potential breeding partner.This searching behavior is coupled with infection risk due to bacterial contamination of the fecal and urine scent marks.We hypothesized that sniffing of female soiled bedding induced the migration of immuno-competent cells into airways as a possible adaptation to breeding-related infection.Using bronchoalveolar lavage in a study on mice,we found the number of leukocytes to be significantly higher in male mice that were provided new portions of soiled bedding daily from female cages,in comparison with male mice that were kept in isolation from female scent.The number of leukocytes in blood was equal in both groups.However,monocytes were fewer in number in male mice exposed to female scent than in male mice isolated from female mice.Scent-induced migration of leukocytes was accompanied by typical behavioral(increased sniffing activity and aggressiveness)and morpho-logical(increase preputial glands and seminal vesicles)responses to olfactory sexual stimulus.展开更多
基金financially supported by the National Natural Science Foundation of China(Grant No.31772338)the Basic Scientific Research Business Special Project of Jiangsu Academy of Agricultural Sciences(Grant No.0090756100ZX)。
文摘Jasmine(Jasminum sambac Aiton)is a well-known cultivated plant species for its fragrant flowers used in the perfume industry and cosmetics.However,the genetic basis of its floral scent is largely unknown.In this study,using PacBio,Illumina,10×Genomics and highthroughput chromosome conformation capture(Hi-C)sequencing technologies,a high-quality chromosome-level reference genome for J.sambac was obtained,exploiting a double-petal phenotype cultivar‘Shuangbanmoli’(JSSB).The results showed that the final assembled genome of JSSB is 580.33 Mb in size(contig N50=1.05 Mb;scaffold N50=45.07 Mb)with a total of 39618 predicted protein-coding genes.Our analyses revealed that the JSSB genome has undergone an ancient whole-genome duplication(WGD)event at 91.68 million years ago(Mya).It was estimated that J.sambac diverged from the lineage leading to Olea europaea and Osmanthus fragrans about 28.8 Mya.On the basis of a combination of genomic,transcriptomic and metabolomic analyses,a range of floral scent volatiles and genes were identified involved in the benzenoid/phenylpropanoid and terpenoid biosynthesis pathways.The results provide new insights into the molecular mechanism of its fragrance biosynthesis in jasmine.
文摘Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions, 5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA （RAPD） and inter-simple sequence repeat （ISSR） marker systems. The varieties analyzed by 11 RAPD and 8 ISSR primers yielded an average of 65% and 80% polymorphism, respectively. The average number of polymorphic bands generated per RAPD primer was 6 and per ISSR primer was 5.87. RAPD and ISSR data analysis individually could not segregate basmati and non-basmati scented rice accessions. However, the analysis using a combined data could group basmati and non-basmati scented rice accessions separately. The bands present specifically among three accessions of non-basmati scented rice were also identified. The study revealed a high genetic diversity among non-basmati scented rice accessions.
基金Supported by Special Fund for Basic Scientific Research Project in Chinese Academy of Tropical Agricultural Sciences(1630032014031)Special Fund for Scientific and Technological Project of Chinese Academy of Tropical Agricultural Science"Research on Selection of High Efficient Varieties of Grain Crops in Hot Region"(0315012)
文摘Containing higher aromatic composition,scented rice is better than ordinary rice varieties in taste and nutrition. Major composition of scented rice is 2-acetyl-1-pyrroline( 2-AP). Different scented rice varieties have different content of 2-AP. The content of 2-AP is not only influenced by genetic factors,but also closely connected with types and content of nutrition elements in soil,soil moisture management,environmental temperature,and storage. 2-AP synthesis and regulation are possibly connected with Proline and enzyme.
文摘This novel rice line is developed recently’by a research team led by Associate Prof QIU Baiqin, Crop Breeding and Cultivation Institute, Zhejiang Academy of Agricultural Sciences. Zhe 9248 is of high resistance to blast, early mature and of higher yield. Being soft, smooth, and glossy, the cooked rice of "Zhe 9248" has excellent eating quality. The kernels of "Zhe 9248" are hardened to such a degree that they could be capa-
文摘We analyzed the inheritance of fragrance bysoaking rice grains or leafblades in 17g/l KOHsolution, using scented rice (Shengxiangjing 4)and non-scented rice (Huxuan 19 and Lao-laiqing) as materials. The Fplants of various crosses did notpresent fragrance. In the Fthe segregating ra-tio of fragrance and non-fragrance plants and
文摘为探明室温贮藏对花果香红茶风味品质和生化组成的影响,本研究比较了4种不同年份(0、1、2、3a)花果香红茶风味感官品质,并采用顶空固相微萃取-气相色谱-质谱联用技术和超高效液相色谱-静电场轨道阱高分辨质谱对花果香红茶中挥发性化合物和非挥发性化合物进行检测。结果表明,室温贮藏对花果香红茶的感官品质影响显著,室温贮藏3 a茶叶失去花果香品质特征,以陈酸等品质特征为主。基于主成分分析和系统聚类分析均可将供试茶样划分成不同年份的4个类群,基于偏最小二乘判别分析和单因素方差分析可从不同贮藏年份花果香红茶中筛选出芳樟醇、顺式-β-罗勒烯、己酸等15种挥发性差异化合物(变量投影重要性(variable importance in project,VIP)>1和P<0.05),茶氨酸、表儿茶素、葡萄糖酸等154种非挥发性差异化合物(VIP>2和P<0.05)。挥发性化合物中芳樟醇、己醛、顺式-β-罗勒烯、2-戊基呋喃等的含量随贮藏年份增加而减少,而己酸、二氢猕猴桃内酯、1-乙基-2-甲酰基吡咯、反式-β-紫罗兰酮等的含量随贮藏年份增加而增加。非挥发性化合物中大部分氨基酸类、核苷酸类、糖类、茶多酚等的含量随贮藏时间延长而减少,而有机酸和脂类化合物等的含量多呈增加趋势。研究结果可为阐明花果香红茶贮藏过程的风味品质变化提供科学依据,也有利于引导市场理性存茶和科学消费。
基金supported by the Russian Foundation for Basic Research(No.08-04-00406)the Heiwa Nakajima Foundation(Japan)。
文摘The scent of receptive females as a signal to reproduction stimulates male mice to olfactory search of a potential breeding partner.This searching behavior is coupled with infection risk due to bacterial contamination of the fecal and urine scent marks.We hypothesized that sniffing of female soiled bedding induced the migration of immuno-competent cells into airways as a possible adaptation to breeding-related infection.Using bronchoalveolar lavage in a study on mice,we found the number of leukocytes to be significantly higher in male mice that were provided new portions of soiled bedding daily from female cages,in comparison with male mice that were kept in isolation from female scent.The number of leukocytes in blood was equal in both groups.However,monocytes were fewer in number in male mice exposed to female scent than in male mice isolated from female mice.Scent-induced migration of leukocytes was accompanied by typical behavioral(increased sniffing activity and aggressiveness)and morpho-logical(increase preputial glands and seminal vesicles)responses to olfactory sexual stimulus.
