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Analysis of Essential Oil from Stems of Schisandra Chinensis(Turc z.) Baill 被引量:2
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作者 LIHong-jun MENGXiang-ying WUYin BAOYong-li LIYu-xin 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2004年第3期311-313,共3页
The essential oil of Schisandra Chinensis(Turc z.) Baill was extracted and analyzed. The essential oil was obtained in a 1.8%(mL/100 g material) yield. Forty eight compounds were isolated and characterized from the oi... The essential oil of Schisandra Chinensis(Turc z.) Baill was extracted and analyzed. The essential oil was obtained in a 1.8%(mL/100 g material) yield. Forty eight compounds were isolated and characterized from the oil. The results show that the content(20.42%) of β -pinene of naturally essential oil is the highest among the components of the oil,which has laid a foundation for the futher development and application of the stems of Schisandra Chinensis(Turc z.) Baill . 展开更多
关键词 schisandra chinensis(Turc z.) Baill Essential oil GC-MS
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Identification of novel mammalian viruses in tree shrews(Tupaia belangeri chinensis)
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作者 Hong Zhou Ren-Rong Tian +9 位作者 Xiu-Rong Wang Jin-Xuan Yang Yun-Xiao Wang Ming-Liang Zhao Xu-Dong Zhang Yu-Hua Ma Long-Bao Lv Edward CHolmes Yong-Tang Zheng Wei-Feng Shi 《Zoological Research》 SCIE CSCD 2024年第2期429-438,共10页
The Chinese tree shrew(Tupaia belangeri chinensis),a member of the mammalian order Scandentia,exhibits considerable similarities with primates,including humans,in aspects of its nervous,immune,and metabolic systems.Th... The Chinese tree shrew(Tupaia belangeri chinensis),a member of the mammalian order Scandentia,exhibits considerable similarities with primates,including humans,in aspects of its nervous,immune,and metabolic systems.These similarities have established the tree shrew as a promising experimental model for biomedical research on cancer,infectious diseases,metabolic disorders,and mental health conditions.Herein,we used metatranscriptomic sequencing to analyze plasma,as well as oral and anal swab samples,from 105 healthy asymptomatic tree shrews to identify the presence of potential zoonotic viruses.In total,eight mammalian viruses with complete genomes were identified,belonging to six viral families,including Flaviviridae,Hepeviridae,Parvovirinae,Picornaviridae,Sedoreoviridae,and Spinareoviridae.Notably,the presence of rotavirus was recorded in tree shrews for the first time.Three viruses-hepacivirus 1,parvovirus,and picornavirus-exhibited low genetic similarity(<70%)with previously reported viruses at the whole-genome scale,indicating novelty.Conversely,three other viruses-hepacivirus 2,hepatovirus A and hepevirus-exhibited high similarity(>94%)to known viral strains.Phylogenetic analyses also revealed that the rotavirus and mammalian orthoreovirus identified in this study may be novel reassortants.These findings provide insights into the diverse viral spectrum present in captive Chinese tree shrews,highlighting the necessity for further research into their potential for crossspecies transmission. 展开更多
关键词 Tree shrew(Tupaia belangeri chinensis) Meta-transcriptomic sequencing Mammalian viruses Genomic analysis
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Cloning and Sequence Analysis of Expansin Genes from Litchi chinensis Fruit 被引量:4
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作者 LUWang-jin JIANGYue-ming 《Agricultural Sciences in China》 CAS CSCD 2003年第6期658-662,共5页
Using PCR degenerate primers, designed with reference to the sequences of the conserved amino acids of known expansins, to amplify cDNA fragments in litchi fruit by RT-PCR, two different cDNA fragments , named as Lc-E... Using PCR degenerate primers, designed with reference to the sequences of the conserved amino acids of known expansins, to amplify cDNA fragments in litchi fruit by RT-PCR, two different cDNA fragments , named as Lc-Exp1 and Lc-Exp2 , were cloned. Lc-Exp1 and Lc-Exp2 was respectively composed of 531 bp encoding 177 amino acids and 537 bp encoding 179 amino acids. Eight cysteine residues and three tryp-tophan residues, which is supposed to be the characteristics of expansins, are conserved in both Lc-Exp1 and c-Exp2. In addition, the homology between the two expansins is 71. 6% at nucleotide acid sequences and 76.3% at amino acid sequences. The homology of Lc-Exp1 with Fa-Exp2 or Pp-Exp1 was 92.7% or 92.1%, but that of Lc-Exp2 with Fa-Exp2 or Pp-Exp1 was only 77. 4% or 76.3% at amino acid sequences. 展开更多
关键词 Litchi chinensis Sonn. fruit EXPANSIN cDNA cloning Sequence analysis
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Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis 被引量:2
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作者 LIU Fengsong LI Fuhua +4 位作者 XIANG Jianhai DONG Bo LIU Yichen ZHANG Xiaojun ZHANG Liusuo 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2008年第2期81-92,共12页
A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fennero- penaeus chinensis by 3 ′and 5′ RACE. The full-length cDNA of Crustin-like gene contains a 3... A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fennero- penaeus chinensis by 3 ′and 5′ RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12. 3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus ( Litopenaeus ) setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary elec- trophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen. 展开更多
关键词 Crustin-like gene Fenneropenaeius chinensis molecular cloning expression analysis microbe challenge
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Establishment of microsatellite-based triplex PCR for parentage analysis of Chinese shrimp Fenneropenaeus chinensis 被引量:4
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作者 GAO Huan KONG Jie +3 位作者 HU Ping MENG Xianhong LUAN Sheng ZHANG Tianshi 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2007年第2期65-74,共10页
Through exploring the microsatellite primers from the random genome sequences of Chinese shrimp (Fenneropenaeus chinensis), some microsatellite primers were obtained with rich polymorphic genetic information, and a ... Through exploring the microsatellite primers from the random genome sequences of Chinese shrimp (Fenneropenaeus chinensis), some microsatellite primers were obtained with rich polymorphic genetic information, and a triplex PCR was established using three primers (RS1101, RS0683 and H081 primers). By adjusting the final concentration of Mg^2+, dNTP and primers, and using a touch-town PCR program, the optimum amplification parameters of PCR system were obtained, which could successfully amplify the three primers in a PCR reaction. In the denatured PAGE gel, the amplified DNA fragments of three primers RS1 101,RS0683 and H081 could be easily identified each other. For the triplex PCR system, the PPE (probabilities of paternity exclusion) is 0.967 9,and the DP (discrimination power) is 0.999 327.Using the triplex PCR to test ten individuals of a parentage and their parents, an individual was excluded from the parentage in all of the three microsatellite loci, which might be mixed into the parentage for some unknown reason such as factitious misplay. The triplex PCR will be of great practical value in identifying the parentages of F. chinensis. 展开更多
关键词 multiplex PCR touch-down PCR microsatellite parentage analysis Fenneropenaeus chinensis
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The Complete Sequence of Mitochondrial COII Gene of Fenneropenaeus chinensis and Its Applicability as a Marker for Phylogenetic Analysis 被引量:4
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作者 YU Shanshan KONG Xiaoyu LI Yulong XU Hui 《Journal of Ocean University of China》 SCIE CAS 2007年第2期187-192,共6页
The complete mitochondrial cytochrome oxidase subunit II (COII) gene of Penaeinae shrimp Fenneropenaeus chinen- sis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%,... The complete mitochondrial cytochrome oxidase subunit II (COII) gene of Penaeinae shrimp Fenneropenaeus chinen- sis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence similarity to Marsupenaeus japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A+T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively. The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those estimated with COI and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COII gene has a faster evolutionary rate than that of the COI gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level. The results of the present study indicated that Farfantepenaeus, Fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the four subgenera to genus level. 展开更多
关键词 中国对虾 线粒体COⅡ基因 完整序列 系统发生分析 标记物
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Cloning and Sequence Analysis of Actin Gene Fragment from Iris lactea var.chinensis Fisch.Koidz
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作者 Fu Guo-hua Yang Tao +1 位作者 Li Wei Wang Jin-gang 《Journal of Northeast Agricultural University(English Edition)》 CAS 2013年第3期12-16,共5页
Degenerate primers were designed based on the conserved sequences of the Actin gene from other plants. Total RNA was extracted from the leaves of lris lacteal var.chinensis Fisch.Koidz. Actin gene fragment was obtaine... Degenerate primers were designed based on the conserved sequences of the Actin gene from other plants. Total RNA was extracted from the leaves of lris lacteal var.chinensis Fisch.Koidz. Actin gene fragment was obtained by reverse transcription polymerase chain reaction (RT-PCR) and cloned into pMD18-T vector. The positive clone identified by PCR was sequenced. The sequencing result showed that the Actin gene fragment from lris lacteal var.chinensis Fisch.Koidz contained about 598 bp, encoding 199 amino acids. Homology comparison with Actin gene sequences of other plants in the GenBank showed that it shared over 82% nueleotide sequence homology and 90% amino acid sequence homology. It indicated that this was the Actin gene. Because of the stability expression ofActin gene, it usually cited as the internal reference to study the expression and regulation of foundation in other genes of lris lacteal var.chinensis Fisch.Koidz well. 展开更多
关键词 Iris lacteal var.chinensis Fisch.Koidz Actin gene CLONING sequence analysis
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Quality Evaluation of Atractylodes chinensis(DC.)Koidz.in Chengde Region Based on Dual Component Analysis
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作者 Xinyuan GAO Hongling ZHAO +3 位作者 Ruyue BAI Zhe LI Jinyue WEI Chunying ZHAO 《Medicinal Plant》 CAS 2021年第5期33-38,41,共7页
[Objectives]To evaluate the quality of Atractylodes chinensis(DC.)Koidz.in Chengde region.[Methods]35 samples of A.chinensis from different growth years were collected,and a high performance liquid chromatography(HPLC... [Objectives]To evaluate the quality of Atractylodes chinensis(DC.)Koidz.in Chengde region.[Methods]35 samples of A.chinensis from different growth years were collected,and a high performance liquid chromatography(HPLC)method was established for the dual components of atractylodin and atractylone,and the quality evaluation was conducted on the cultivated A.chinensis from Chengde region in terms of moisture,ash and volatile oil content.[Results]The moisture content of all samples met the regulations,and the content of volatile oil was relatively high.Among them,seven samples with atractylodin content lower than 0.3%were unqualified products(accounting for 20%),and one sample had excessive ash content(accounting for 3%).[Conclusions]The established method for the determination of the dual components of Atractylodis Rhizoma is stable,simple,fast,precise and reusable.Besides,according to the Pearson correlation analysis among the indicators,it is concluded that the atractylodin and atractylone content are significantly related to the growth period and the volatile oil content.In addition,the quality evaluation indicators of A.chinensis in Chengde region show that there are differences in the quality of Atractylodis Rhizoma,but the overall pass rate is relatively high.It is necessary to further study the reasons for the differences. 展开更多
关键词 Atractylodes chinensis(DC.)Koidz. HPLC Correlation analysis Quality evaluation
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胚乳和外源赤霉素对五味子[Schisandra chinensis (Turcz.) Baill]种子发芽的影响 被引量:12
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作者 李成浩 牛遇达 +1 位作者 刘桂丰 杨传平 《植物生理学通讯》 CSCD 北大核心 2006年第4期625-627,共3页
以组织培养和土壤播种的未经层积处理的五味子种子为材料,检测胚乳和外源赤霉素(GA3)对发芽影响的结果表明,在不加生长调节物质的MS培养基上,去掉外种皮带全胚乳的种子发芽率只有2.7%,而带1/2和1/3胚乳的种子发芽率则分别为4... 以组织培养和土壤播种的未经层积处理的五味子种子为材料,检测胚乳和外源赤霉素(GA3)对发芽影响的结果表明,在不加生长调节物质的MS培养基上,去掉外种皮带全胚乳的种子发芽率只有2.7%,而带1/2和1/3胚乳的种子发芽率则分别为46%和70.6%;培养基中添加GA,,可提高去掉外种皮带全胚乳和带1/2胚乳种子的发芽率,而对带1/3胚乳的种子则无效果。土壤播种时,用10—1000mg·L—GA3对去掉外种皮种子进行1~10d前处理,其中100mg·L—GA3前处理10d的发芽率最高,达到38.7%。 展开更多
关键词 五味子 种皮 发芽 胚乳 赤霉素
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海州湾中国毛虾(Acetes chinensis)种群生物学特征和资源开发状态研究
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作者 吴晓睿 宋大德 +8 位作者 熊瑛 仲霞铭 李纲 杨帆 康中杰 李国东 李冬佳 施金金 闫欣 《海洋与湖沼》 CAS CSCD 北大核心 2023年第2期573-582,共10页
为掌握我国限额捕捞物种海州湾中国毛虾(Acetes chinensis)的资源动态,于2020、2021年6~7月分别采集了1094、1700尾中国毛虾样本,测定其体长、体质量和性别等特征,并运用FiSATⅡ软件对生长、死亡参数及资源利用水平等进行评估。结果显示... 为掌握我国限额捕捞物种海州湾中国毛虾(Acetes chinensis)的资源动态,于2020、2021年6~7月分别采集了1094、1700尾中国毛虾样本,测定其体长、体质量和性别等特征,并运用FiSATⅡ软件对生长、死亡参数及资源利用水平等进行评估。结果显示:2020、2021年限额捕捞期间其平均体长分别为32.30、35.73 mm,雌雄性比分别为1.52︰1、1.62︰1,体长大于35 mm的毛虾中雌性比例分别为98.5%、90.0%,并得出了体质量-体长关系式;2020、2021年自然死亡系数(M)分别为0.55、0.61 month^(-1),捕捞死亡系数(F)分别为0.62、0.27 month^(-1);2020、2021年开捕体长(L_(c))分别为26.28、29.73 mm,均小于最适开捕体长(L_(opt))33 mm;2020、2021年资源开发率(E)分别为0.53、0.31,均小于当年E_(0.1)(0.60、0.77),表明海州湾中国毛虾资源尚未过度捕捞。鉴于我国实施伏季休渔期毛虾限额捕捞的规模不断扩大,应谨慎增加捕捞努力量,将开发率E有效控制在E_(0.1)以内。 展开更多
关键词 中国毛虾 海州湾 体长股分析法 群体生物学特征 开发率
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Effect of Schisandra chinensis polysaccharide on intracerebral acetylcholinesterase and monoamine neurotransmitters in a D-galactose-induced aging brain mouse model 被引量:2
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作者 Mingsan Miao Jianlian Gao +2 位作者 Guangwei Zhang Xiao Ma Ying Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2009年第9期687-693,共7页
BACKGROUND: The most prominent characteristic of brain aging is decreased learning and memory ability. The functions of learning and memory are closely related to intracerebral acetylcholinesterase (ACHE) and monoa... BACKGROUND: The most prominent characteristic of brain aging is decreased learning and memory ability. The functions of learning and memory are closely related to intracerebral acetylcholinesterase (ACHE) and monoamine neurotransmitter activity. Previous studies have shown that Schisandra chinensis polysaccharide has an anti-aging effect. OBJECTIVE: To explore the effects of Schisandra chinensis polysaccharide on AChE activity and monoamine neurotransmitter content, as well as learning and memory ability in a D-galactose-induced aging mouse brain model compared with the positive control drug Kangnaoling. DESIGN, TIME AND SETTING: Completely randomized, controlled experiment based on neurobiochemistry was performed at the Pharmacological Laboratory, Henan University of Traditional Chinese Medicine from September to December 2003. MATERIALS: Schisandra chinensis was purchased from Henan Provincial Medicinal Company. Schisandra chinensis polysaccharide was obtained by water extraction and alcohol precipitation. Kangnaoling pellets were provided by Liaoning Tianlong Pharmaceutical (batch No. 20030804; state drug permit No. H21023095). A total of 50 six-week-old Kunming mice were randomly divided into five groups: blank control, model, Kangnaoling, high and low dosage Schisandra chinensis polysaccharide groups, with 10 mice per group. METHODS: Mice in the blank control group were subcutaneously injected with 0.5 mL/20 g normal saline into the nape of the neck each day, while the remaining mice were subcutaneously injected with 5% D-galactose saline solution (0.5 mL/20 g) in the nape for 40 days to induce a brain aging model. On day 11, mice in the high and low dosage Schisandra chinensis polysaccharide groups were intragastrically infused with 20 mg/mL and 10 mg/mL Schisandra chinensis polysaccharide solution (0.2 mL/10 g), respectively. Mice from the Kangnaoling group were intragastrically infused with 35 mg/mL Kangnaoling suspension (0.2 mL/10 g), and the mice in the model group were intragastrically infused with the same volume of normal saline (0.2 mL/10 g) once per day for 30 consecutive days. MAIN OUTCOME MEASURES: Two hours after the final administration, pathohistological changes in the cerebral cortex and hippocampus were observed using hematoxylin & eosin staining. AChE activity was detected using chromatometry. Monoamine neurotransmitter content was measured using fluorimetry. Learning and memory was measured using the step down test and darkness avoidance test. RESULTS: Both Schisandra chinensis polysaccharide and Kangnaoling improved pathological injury to the cerebral cortex and hippocampus in a mouse model of brain aging. Compared with the blank control group, AChE activity and content of norepinephrine (NA), dopamine (DA), and 5-hydroxytryptamine (5-HT) were significantly decreased in the model group (P 〈 0.01 ). In contrast, AChE activity and NA, DA, and 5-HT levels significantly increased in the Kangnaoling and high dosage Schisandra chinensis polysaccharide groups (P 〈 0.01), while NA levels significantly increased in the low dosage Schisandra chinensis polysaccharide group (P 〈 0.01). Drug treatment improved learning and memory abilities (P 〈 0.01 or P 〈 0.05). CONCLUSION: Schisandra chinensis polysaccharide significantly increased levels of central neurotransmitters and improved learning and memory in a mouse model of brain aging. The effects of Schisandra chinensis polysaccharide were equal to that of Kangnaoling pellets. 展开更多
关键词 schisandra chinensis polysaccharide D-GALACTOSE brain aging NEUROTRANSMITTER acetylcholine esterase
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Analysis of Nutrition Composition of Schisandra Fruits 被引量:1
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作者 孙吉虹 袁晓颖 +1 位作者 赵秋雁 朱虹 《Journal of Forestry Research》 SCIE CAS CSCD 1997年第2期97-98,共2页
A systematically analysis of nutritional composition of Schisandra chinensis and appreciation on its value of utilization were made for exploring a new way of utilization. The results showed that the fruit of Schisand... A systematically analysis of nutritional composition of Schisandra chinensis and appreciation on its value of utilization were made for exploring a new way of utilization. The results showed that the fruit of Schisandra contains rich in kinds of vitamins, amino acids, organic acids and microelements that fit with human body. Its kemal contains the fatty ac-ids with high value of utilization. Linoleic acid accounts for 50.7% of the amount of fatty acids in in kemel. The contents ofthe trace elemat Fe, Zn are also higher. Ve's content of Schisandra is 8 times of the orange. 展开更多
关键词 Schisander chinensis Chemical composition Nutrition analysis
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Nortriterpenoids from the fruit stalk of Schisandra chinensis (Turcz.) Baill. 被引量:1
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作者 Yan Liu Xiaohui Rong +4 位作者 Yuanhang Chang Juan Pan Wei Guan Haixue Kuang Bingyou Yang 《Frigid Zone Medicine》 2022年第2期103-108,共6页
Objective:The fruit stalk of Schisandra chinensis(Turcz.)Baill.(S.chinensis)has been found to contain bioactive components similar to the fruit of S.chinensis.Here,we report a recent discovery about new nortriterpenoi... Objective:The fruit stalk of Schisandra chinensis(Turcz.)Baill.(S.chinensis)has been found to contain bioactive components similar to the fruit of S.chinensis.Here,we report a recent discovery about new nortriterpenoids with a novel skeleton and anti-gastric cancer activity,which were isolated from the fruit stalk of S.chinensis.Methods:The chemical components of ethyl acetate extract from 70%ethanol extract from S.chinensis fruit stalk were separated,purified,and identified by liquid chromatography methods(silica gel,ODS,HPLC)and extensive spectroscopic analyses(NMR,IR,UV,MS,CD).Results:Two new nortriterpenoids,schilancitrilactone M and 25-hydroxyl schindilactone D(1 and 2),along with ten known nortriterpenoids(3-12)were isolated from the fruit stalk of S.chinensis.The isolated compounds were tested for their cytotoxic activities against MGC-803 cells,and the results showed that compounds 6-8 possessed significant activities with IC50 of 9.01,11.77,and 2.74μmol/L,respectively.Conclusion:Twelve nortriterpenoids including two new compounds were isolated from the fruit stalk of S.chinensis for the first time.Among them,compounds 6-8 showed significant anti-gastric cancer activities.We postulated that the fruit stalk of S.chinensis could be used as an anti-gastric cancer drug. 展开更多
关键词 nortriterpenoids schisandra chinensis(Turcz.)Baill. fruit stalk CYTOTOXICITY MGC-803 cells
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Study on HPLC method to determine contents of Schisandrin A and Schisandrin B in Schisandra chinensis extraction
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作者 XU Liangmei LI Jianping YAN Changjiang SHAN Anshan 《Journal of Northeast Agricultural University(English Edition)》 CAS 2007年第4期323-326,共4页
The determination method of Schisandrin A and Schisandrin B in Schisandra chinensis was improved with the high performance liquid chromagraphy (HPLC). The sample was extracted exceedingly in the critical limit of CO... The determination method of Schisandrin A and Schisandrin B in Schisandra chinensis was improved with the high performance liquid chromagraphy (HPLC). The sample was extracted exceedingly in the critical limit of CO2. The retention time of Schisandrin A and Schisandrin B was reduced, with methano/water (75 : 25) as mobile phase. The wavelength for detection was 254 nm. The R^2 of standard curve was 0.9998 and the relative standard deviation was 2.31% and 3.17% with the recovery of 96.45% and 97.37%, respectively. The result shows that the rate of veracity of this method is higher and it proves that the determination method of Sehisandrin A and Schisandrin B in Schisandra chinensis is a feasible method. 展开更多
关键词 HPLC schisandra chinensis schisandra A Schisandrin B
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Schisandra chinensis (Turcz.) Baill
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作者 Guo ZJ, Wang XM, Xi’an Jiaotong University, Xi’an, China 《Journal of Pharmaceutical Analysis》 SCIE CAS 2012年第4期240-240,共1页
Schisandra chinensis (Turcz.) Baill. is a deciduous woody vine, and belongs to the Schisandraceae family. This species is a horticultural plant with edible fruit. It is used medicinally and for making rope. It is dist... Schisandra chinensis (Turcz.) Baill. is a deciduous woody vine, and belongs to the Schisandraceae family. This species is a horticultural plant with edible fruit. It is used medicinally and for making rope. It is distributed in Hebei, Heilongjiang, Jilin, Liaoning, Nei Mongol, Shanxi 展开更多
关键词 schisandra chinensis Baill Turcz
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Pharmacological effects of Schisandra Chinensis Fructus polysaccharide on intestinal protection
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作者 LI Ming-song ZHANG Dan-shen JING Yong-shuai 《中国药理学与毒理学杂志》 CAS 北大核心 2021年第10期766-767,共2页
Schisandra Chinensis Fructus(SCF)is the fruit of Schisandra chinensis(Turcz.)Baill.,a perennial vine.It was first recorded in Shen Nong′s herbal classic and has a long application history.Studies have shown that SCF ... Schisandra Chinensis Fructus(SCF)is the fruit of Schisandra chinensis(Turcz.)Baill.,a perennial vine.It was first recorded in Shen Nong′s herbal classic and has a long application history.Studies have shown that SCF has anti-inflammatory,protective liver,antioxidant,antibacterial and other pharmacological effects.Ancient prescriptions are commonly used in the treatment of chronic diarrhea and other intestinal diseases and diabetes.Modern clinical pharmacology features of SCF polysaccharide(SCFP)in diabetes,liver diseases,enteritis and other aspects have achieved excellent results.Gut is an important digestive organ of human body,but intestinal diseases are varied,including Crohn′s disease,ulcerative colitis,intestinal flora imbalance,etc..It is a chronic and non-specific inflammatory disease.The disease is persisted for a long time and the incidence rate is expected to rise.Most of the symptoms are recurrent diarrhea,bloody stool and abdominal pain.It is considered by the World Health Organization as a refractory disease.At present,there is little possibility of complete cure,which is closely related to complex environmental factors,eating habits and heredity.In recent years,clinical studies have found that SCFP has a variety of pharmacological effects on intestinal protection.①Reduce inflammatory factors:intestinal mucositis is a common adverse reaction in patients with chemotherapy.The development of mucositis is related to pro-inflammatory factors such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),IL^(-1)β,Interferon-γ(IFN-γ).SCFP can significantly reduce IL-6 TNF-α,IL^(-1)β,and IL-8,as well as the accumulation of T cells in the process of resisting apoptosis,reduce the inflammatory reaction and protect the damage to villi and crypts,improve the symptoms of small intestinal mucositis caused by weight loss and diarrhea.②Promote immunoglobulin A secretion:intestinal mucosal immunity is the first line of defense of the body′s immune system.Its main antibody is secretory immunoglobulin A,which can destroy and phagocytize microorganisms,bacteria and viruses.SCFP can improve intestinal immunity by increasing the number and activity of T lymphocytes,promoting the secretion of secretory immunoglobulin A,and affecting the activity of a variety of cytokines.③Regulation of intestinal flora:the flora in the intestine has the functions of auxiliary nutrient absorption,biological antagonism and immune regulation,and can form a natural barrier for the host's intestine.When the human intestinal flora is disordered,probiotics will be greatly reduced,harmful bacteria will proliferate and destroy the intestinal environment.Under these conditions,the intake of SCFP significantly increased the number of beneficial bacteria such as bifidobacteria and lactobacillus,and significantly decreased the number of conditional pathogens such as enterococcus and escherichia coli,indicating that SCFP can indeed regulate the intestinal disorder caused by lincomycin hydrochloride to a certain extent. This may be because beneficial bacteria in the intestine metabolize polysaccharides produce short chain fatty acids such as lactic acid and acetic acid, which reduces the pH value in the intestine and inhibits the growth of enterococcus and Escherichia coli. In conclusion, SCFP can treat and protect intestinal diseases to a certain extent, which provides a favorable basis for the treatment of intestinal diseases. 展开更多
关键词 schisandra chinensis Fructus POLYSACCHARIDE intestinal diseases inflammatory factor
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Metabolism alternation of schisandra chinensis in carbon tetrachloride-intoxicated rats and patients with advanced hepatocellular carcinoma
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作者 Rong-rong WU Zhi-yong XIAO +3 位作者 Xiao-rui ZHANG Feng LIU Wen-xia ZHOU Yong-xiang ZHANG 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2018年第4期314-314,共1页
OBJECTIVE To study the pharmacokinetics change of schisandra chinensis under the pathological condition of liver dysfunction for safe and rational use of herbal medicines.METHODS The metabolism of four effective ligna... OBJECTIVE To study the pharmacokinetics change of schisandra chinensis under the pathological condition of liver dysfunction for safe and rational use of herbal medicines.METHODS The metabolism of four effective lignans from schisandra chinensis(SC),schisandrin,schisantherin A,deoxyshisandrin and γ-schisandrin was studied using microsomes from patients with advanced hepato.cellular carcinoma.In situ intestinal and hepatic perfusions were conducted to clarify the contributions from impairments of gut and liver on the pharmacokinetics of the four schisandra lignans in CCl4-intoxi.cated rats.The metabolism in rat and human liver microsomes and transport in Caco-2 monolayer cell model were studied to reveal the key factors for the in vivo disposition of the four lignans.RESULTS When SC alcoholic extract was orally administrated to CCl4-intoxicated rat for a short term(4 d),the pharmacokinetics of four active SC lignans was significantly changed while its hepatoprotective effect was not obviously observed.The plasma concentrations of the four schisandra lignans were dramatical.ly elevated compared with the control.The Cmax,AUC and MRT were all increased or prolonged signif.icantly while parameter CLz/F was obviously reduced in rat pretreated with CCl4.In hepatic perfusion study and liver microsomes incubation,it was found that the hepatic metabolism of the four lignans was markedly decreased mainly due to the activity reduction of multiple CYP450 isoenzymes involved the metabolism,which,eventually,might lead to the alternation of their pharmacokinetic profiles in CCl4-intoxicated rats or patients with advanced hepatocellular carcinoma.CONCLUSION The pharmacoki.netic studies of SC components in pathological situation of liver dysfunction are expected to provide useful data for rational and safe application of SC preparations in clinic or further pharmacological and toxicological research. 展开更多
关键词 五味子 肝功能 治疗方法 临床分析
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中国对虾(Fenneropenaeus chinensis)黄、渤海3个野生地理群遗传多样性的微卫星DNA分析 被引量:16
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作者 刘萍 孟宪红 +3 位作者 何玉英 孔杰 李健 王清印 《海洋与湖沼》 CAS CSCD 北大核心 2004年第3期252-257,共6页
运用微卫星DNA技术 ,以中国对虾渤海湾群体 (BH)、辽东湾群体 (LD)和海州湾群体(HZ)的 3个野生群共计 60个个体为实验材料 ,进行了 7个基因位点的遗传参数分析。同时对7对微卫星引物的多态性信息含量 (PIC)进行了评估 ,EN0 0 2 1位点提... 运用微卫星DNA技术 ,以中国对虾渤海湾群体 (BH)、辽东湾群体 (LD)和海州湾群体(HZ)的 3个野生群共计 60个个体为实验材料 ,进行了 7个基因位点的遗传参数分析。同时对7对微卫星引物的多态性信息含量 (PIC)进行了评估 ,EN0 0 2 1位点提供的信息含量低于 0 5 ,其他 6对微卫星引物的PIC值均在 0 5以上 ,适于应用于中国对虾的群体分析。结果表明 ,这7个微卫星位点在中国对虾 3个地理群 60个样品的分析中共获得了 5 6个等位基因 ,对 3个野生群体的 7个基因位点共计 2 1个群体位点进行了杂合度观测值 (Ho)和杂合度期望值 (He)计算 ;在Hardy Weinberg平衡条件下 ,进行了P检验 ,发现BH和HZ各有 1个群体位点发生平衡偏离 ,而LD有 1个群体位点发生平衡偏离 ,还有 2个群体位点已发生显著平衡偏离。但除此之外 ,对 3个地理群间的遗传分化指数Fst值进行的计算结果表明 ,BH和LD之间遗传分化较弱 ,HZ与另 2个地理群间则产生了中等程度的分化。从变异贡献率来看 ,有 92 83%的遗传变异来自个体之间 ,只有 7 1 7%的遗传变异来自群体之间。经过UPGMA聚类 ,发现BH群体和LD群体的亲缘关系较近 ,而HZ群体与前两者亲缘关系较远。 展开更多
关键词 中国对虾 地理群体 微卫星DNA 多态性分析
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太白红杉(Larix chinensis)叶的形态解剖学特征与环境因子的关系 被引量:37
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作者 刘全宏 王孝安 +1 位作者 田先华 肖娅萍 《西北植物学报》 CAS CSCD 2001年第5期885-893,共9页
根据生态气候学方法推断出不同海拔高度气候指标 ,应用多元统计方法 ,对分布于秦岭太白山地区海拔 30 0 0~ 340 0 m的太白红杉 ( L arix chinensis)叶特征进行了生态解剖学研究 ,分析了影响其变化的主导生态因子。观察指标包括叶形态指... 根据生态气候学方法推断出不同海拔高度气候指标 ,应用多元统计方法 ,对分布于秦岭太白山地区海拔 30 0 0~ 340 0 m的太白红杉 ( L arix chinensis)叶特征进行了生态解剖学研究 ,分析了影响其变化的主导生态因子。观察指标包括叶形态指标 (叶片总厚度、每束叶中的叶数和叶长宽比 )、叶解剖学特征 (叶表皮角质膜厚度 ,上、下表皮厚度 ,近上、下表皮叶肉厚度 ,内皮层厚度 ,管胞直径 ,叶转输组织和维管束厚度及其与叶片总厚度之比 )。结果表明 :1水热综合因子是影响太白红杉叶长宽比和叶表皮角质膜厚度的主导因子 ,随着水热综合因子的增大 ,叶长宽比呈增加趋势 ,而角质膜厚度则逐渐变薄。2年降水量是影响其它形态解剖学指标的主导因子 ,随着年降水量的增加 ,每束叶中的叶数、叶厚度、管胞直径、叶转输组织和维管束厚度、内皮层厚度、转输组织和维管束厚度及其与叶总厚度之比呈减少和变薄的趋势 ,而上、下表皮厚度则逐渐增加。 3近上、下表皮叶肉厚度与环境因子无明显相关 。 展开更多
关键词 太白红杉 形态解剖 环境因子 多元统计分析
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北五味子(Schizandra Chinensis Baillon)的营养学研究 被引量:3
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作者 杜延茹 卢宝臣 +1 位作者 陈思学 姚忠文 《哈尔滨师范大学自然科学学报》 CAS 1992年第4期84-86,共3页
北五味子(Schizandra Chinensis Baillon)分布在东北东部山区,在林下林间大量生长,野生资源丰富。是我国东北地道的中草药,并为医药学家视为佳品。前人对五味子的药理学、成药学作了大量的研究工作,但关于它的营养学研究尚未见报导。作... 北五味子(Schizandra Chinensis Baillon)分布在东北东部山区,在林下林间大量生长,野生资源丰富。是我国东北地道的中草药,并为医药学家视为佳品。前人对五味子的药理学、成药学作了大量的研究工作,但关于它的营养学研究尚未见报导。作者利用先进的科学仪器定量测定了原汁的各种营养素的含量。结果表明五味子营养素齐全,维生素、微量元素、有机酸含量丰富,是制造天然保健饮料的优秀原料。 展开更多
关键词 五味子 营养学 北五味子
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