The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis f...The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis for clinical use. The potential of second-order calibration method was validated for the quantification of two effective ingredients of Schisandra chinensis in human plasma using spectrofluorimetry. The results obtained in the present study demonstrate the advantages of this strategy for multi-target determination in complex matrices. Although the spectra of the analytes are similar and a large number of interferences also exist, second-order calibration method could predict the accurate concentrations together with reasonable resolution of spectral profiles for analytes of interest owing to its ‘second-order advantage’. Moreover, the method presented in this work allows one to simply experimental procedure as well as reduces the use of harmful chemical solvents.展开更多
目的建立反相高效液相色谱法同时测定五味子中6种木脂素含量的方法,并应用此方法检测南、北五味子及3种市售成药:参芪五味子片、安神补心片、安神补心胶囊中此6种木脂素的含量。方法采用SHIMADZU VP-ODS(4.6 mm×150 mm i.d,5μm)...目的建立反相高效液相色谱法同时测定五味子中6种木脂素含量的方法,并应用此方法检测南、北五味子及3种市售成药:参芪五味子片、安神补心片、安神补心胶囊中此6种木脂素的含量。方法采用SHIMADZU VP-ODS(4.6 mm×150 mm i.d,5μm)色谱柱,以乙腈-水为流动相,进行梯度洗脱,流速0.5mL/min,检测波长220 nm,柱温30℃。结果五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素分离度良好,分别在0.243~2.430μg、0.087~0.870μg、0.060~0.600μg、0.093~0.930μg、0.246~2.460μg、0.063~0.630μg的进样量范围内有良好的线性关系,平均加样回收率分别为100.93%、100.59%、99.78%、100.43%、96.72%、102.33%,RSD值分别为2.14%、2.95%、2.26%、2.81%、1.88%、0.985%。结论本研究所建立的反相高效液相色谱法方法准确可靠、简单可行,可用于五味子中木脂素类成分的定量分析,为五味子药材及以五味子为组方药味的中成药的质量评价奠定基础。展开更多
基金the National Natural Science Foundation of China (Grant No. 21175041)the National Basic Research Program (Grant No. 2012CB910602)Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) for financial supports
文摘The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis for clinical use. The potential of second-order calibration method was validated for the quantification of two effective ingredients of Schisandra chinensis in human plasma using spectrofluorimetry. The results obtained in the present study demonstrate the advantages of this strategy for multi-target determination in complex matrices. Although the spectra of the analytes are similar and a large number of interferences also exist, second-order calibration method could predict the accurate concentrations together with reasonable resolution of spectral profiles for analytes of interest owing to its ‘second-order advantage’. Moreover, the method presented in this work allows one to simply experimental procedure as well as reduces the use of harmful chemical solvents.
