Studies on the Physicochemical Properties, Structure and Antitumor Activity of an Oligosaccharide Homologue SnS-2 from the Root of Scrophularia ningpoensis Hemsl.

An oligosaccharide homologue named SnS-2 was isolated from the root of Scrophularia ningpoensis Hemsl. SnS-2 was purified by means of gel-permeation chromatography and ion-exchange chromatography. Its physico-chemical properties, including carbohydrate content and molecular weight were determined. The structure of SnS-2 was elucidated by chemical methods along with 1H and 13C NMR spectroscopy, including two-dimensional DQCOSY and H-detected 1H, 13C HMQC experiments. These results show that SnS-2 possesses a backbone con-sisting of terminal a-Galp-(1, a-Galp-(16), a-Glcp-(16) and nonreducing end -Fruf-(2. The bioactive as-say showed that it could inhibit the growth of Lewis pulmonary carcinoma implanted in mice.

Iridoid glycosides from the roots of Scrophularia ningpoensis Hemsl

Two new iridoid glycosides,named scrophularianoids A（1） and B（2）,were isolated from the roots of Scrophularia ningpoensis.The chemical structures were established on the basis of extensive analyses of spectroscopic data.Compounds 1 and 2 were inactive in our preliminary in vitro myocardial protective bioassay.

Molecular authentication of geo-authentic Scrophularia ningpoensis

Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation.Like other herbal medicines,S.ningpoensis collected from different localities may considerably differ in their therapeutic efficacy,and the one grown in Zhejiang Province is recognized as geo-authentic.However,it is difficult to confirm the geo-graphical authenticity by similar morphological characteristics.In the present study,inter-simple sequence repeat(ISSR) markers were conducted to detect S.ningpoensis from different origins.A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones.By cloning and sequencing that specific band,sequence characterized amplified region(SCAR) markers were designed to distinguish geo-authentic S.ningpoensis from others.This is a rapid and easy method that can be used to identify the geographical authenticity of S.ningpoensis.

HPLC Determination of Harpagoside and Cinnamic Acid in Radix Scrophulariae

A gradient HPLC method was established for the determination of harpagoside and cinnamic acid in Radix Scrophulariae (Xuanshen) and a proposition was put forward for the lowest content of the characteristic and active constituent (harpagoside) for qualified Radix Scrophulariae. The experimental conditions were as follows: Ultrasphere ODS column (250 mm 4.6 mm, 5 mm), mobile phase: acetonitrile-water (containing 1.0% acetic acid) (20:8050:50) (20 min), flow-rate 1 mLmin-1, room temperature, detection wavelength 278 nm. Twenty-eight samples of Xuan-shen (Radix Scrophulariae) from different districts of China were analyzed and the contents of harpagoside and cinnamic acid in Xuan-shen were 0.041~0.244% and 0.012~0.068% respectively. The recoveries (RSD)% were 97.13(0.80)% for harpagoside and 99.38(0.51)% for cinnamic acid. The method is simple and accurate. It can be used for the quality control of Radix Scrophulariae. We propose that the content of harpagoside in qualified Radix Scrophularia should be no less than 0.05%.

施肥对玄参生长及品质的影响

以玄参(Scrophularia ningpoensis Hemsl.)、微生物菌肥、氨基酸硼锌肥为试验材料,对玄参分别施用氨基酸硼锌肥、微生物菌肥,统计分析玄参地上部分、地下部分生长情况,病虫害发生率,药材有效成分含量等指标,研究了不同肥料对玄参生长、产量和质量的影响,以期为科学种植玄参提供参考。结果表明,微生物菌肥、氨基酸硼锌肥对玄参植株、药茎、子芽生长有促进作用,对斑枯病等病害具有抑制作用,微生物菌肥对地下部分生长和病害抑制效果比氨基酸硼锌肥更好,但对地上部分的促进作用不及氨基酸硼锌肥,微生物菌肥、氨基酸硼锌肥对玄参药材中有效成分哈巴苷含量具有明显的提高作用,微生物菌肥、氨基酸硼锌肥处理分别可提升至对照的2.46、2.10倍。综合植株生长、产量、抗病性等因素,可配施微生物菌肥、氨基酸硼锌肥和化肥,达到高产、高抗、高质的目的,生产出道地好药材。

Harpagometabolins Ⅰ and Ⅱ, Two New Metabolites from Harpagoside by Human Intestinal Bacteria

Harpagoside, which is one main iridoid constituent of the dried roots of Scrophularia ningpoensis Hemsl., was biotransformated by bacteria isolated from human feeal flora a.d three metabolites were obtained. The structures of the metabolites, including two new alkaloids, named bar'Pagometabolins Ⅰ (l) and Ⅱ (2), and a known alkaloid acubinine B(3), were identified by chemical methods and the spectroscopic evidences.

Structural characterization and identification of major constituents in Radix Scrophulariae by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry

AIM: To analyze the major constituents in Radix Scrophulariae(Scrophularia ningpoensis). METHOD: Radix Scrophulariae was analyzed by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry(ESI-Q-TOF MS/MS). Compounds were separated by HPLC using a C18 column and gradient elution of acetonitrile and 0.1 %(V/V) acetic acid-water. Negative ion mode was employed. RESULTS: A total of thirty-six compounds, including fourteen iridoid glycosides, nineteen phenylpropanoid glycosides, and three organic acids, were identified from Radix Scrophulariae based on the accurate mass measurement of precursor and product ions. Twenty-one of the constituents were identified by comparing their retention times(tR) and ESI-MS/MS data with those of reference standards and/or previous publications, while another fifteen compounds were tentatively identified or deduced according to their Q-TOF MS/MS data which afforded sufficient structural information. CONCLUSION: It is believed that this study is useful for the identification of constituents in Radix Scrophulariae, as well as related plants and complex prescriptions.