[Objective] To utilize the germplasm resources,new systematic evidence for the classification of sect.Paracamellia,genus Camellia is found in this article.[Method] Fruit anatomy of 10 species representing sect.Paracam...[Objective] To utilize the germplasm resources,new systematic evidence for the classification of sect.Paracamellia,genus Camellia is found in this article.[Method] Fruit anatomy of 10 species representing sect.Paracamellia was investigated.And the main component of sect.Paracamellia in the genus Camellia was studied by MVSP software.[Result] The exocarp was thin;mesocarp was broad,having a large number of stone cells;endocarp was lignified,and separated easily from seed.The principle components analysis suggested that the distance between species was far,showing far relation;the current study did not support the formation of Camellia grijsii（C.grijsii）and Camellia yuhsienensis（C.yuhsienensis）,Camellia shensiensis（C.shensiensis）should not be recognized as a variety of C.yuhsienensis.In addition,it also proved Ming＇s combining of Camellia confusa（C.confusa）and Camellia kissi（C.kissi）was unnatural.[Conclusion] The study provided theoretical basis for the development and application of sect.Paracamellia in the genus Camellia.展开更多
Osmotic stress caused by low-temperature,drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield,particularly in tea plant.Jasmonic acid(JA)is an i...Osmotic stress caused by low-temperature,drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield,particularly in tea plant.Jasmonic acid(JA)is an important phytohormone involving in plant stress.However,underlying molecular mechanisms of JA modulated osmotic stress response remains unclear.In this study,high concentration of mannitol induced JA accumulation and increase of peroxidase activity in tea plant.Integrated transcriptome mined a JA signaling master,MYC2 transcription factor is shown as a hub regulator that induced by mannitol,expression of which positively correlated with JA biosynthetic genes(LOX and AOS)and peroxidase genes(PER).CsMYC2 was determined as a nuclei-localized transcription activator,furthermore,ProteinDNA interaction analysis indicated that CsMYC2 was positive regulator that activated the transcription of CsLOX7,CsAOS2,CsPER1 and CsPER3via bound with their promoters,respectively.Suppression of CsMYC2 expression resulted in a reduced JA content and peroxidase activity and osmotic stress tolerance of tea plant.Overexpression of CsMYC2 in Arabidopsis improved JA content,peroxidase activity and plants tolerance against mannitol stress.Together,we proposed a positive feedback loop mediated by CsMYC2,CsLOX7 and CsAOS2 which constituted to increase the tolerance of osmotic stress through fine-tuning the accumulation of JA levels and increase of POD activity in tea plant.展开更多
Objective:To investigate the antifungal potential of different grades of Sri Lankan orthodox black tea [orange pekoe, broken orange pekoe fannings (BOPF) and Dust No. 1] belonging to the three agro-climatic elevations...Objective:To investigate the antifungal potential of different grades of Sri Lankan orthodox black tea [orange pekoe, broken orange pekoe fannings (BOPF) and Dust No. 1] belonging to the three agro-climatic elevations (low, mid and high). Methods: Antifungal activity was assessedin vitro using methanolic extracts (300μg/disc) and agar disc diffusion bioassay technique against threeCandida species,Candida albicans (C. albicans),Candida glabrata(C. glabrata), andCandida tropicalis. ketoconazole and itraconazole mixture was used as positive control (10μg/disc) and methanol was used as the negative control. The minimum inhibitory concentrations were also determined using standard protocols. Results: None of the extracts were effective againstCandida tropicalis. Furthermore, orange pekoe grade tea belonging to all agro-climatic elevations did not induce any antifungal activity againstC. albicans andC. glabrata as well. Conversely, Dust No. 1 belonging to all three agro-climatic elevations and low-grownBOPF showed moderate antifungal activity againstC. albicans andC. glabrata. Interestingly, the severity of the antifungal effect varied with agro-climatic elevations. The minimum inhibitory concentrations ranged from 64.00–128.00μg/mL againstC. glabrata and 128.00-256.00μg/mL againstC. albicans. Conclusions:Sri Lankan Dust No. 1 andBOPF have marked antifungal activityin vitro and offer promise to be used as a supplementary beverage in prophylaxis and during drug treatment in candidiasis.展开更多
描述了山茶属短柱茶组新种Camellia osmantha Ye CX,Ma JL et Ye H.新种为灌木,花白色,微有香气。其苞被不分化为苞片和萼片,多数,10～12枚,花瓣6～8枚,均易脱落;雄蕊长短不一,大部分分离;花柱短,基部连生,先端分离,与短柱茶组特征一致...描述了山茶属短柱茶组新种Camellia osmantha Ye CX,Ma JL et Ye H.新种为灌木,花白色,微有香气。其苞被不分化为苞片和萼片,多数,10～12枚,花瓣6～8枚,均易脱落;雄蕊长短不一,大部分分离;花柱短,基部连生,先端分离,与短柱茶组特征一致。新种花有香气与窄叶短柱茶C.fluviantlis Hand.-Mzt.接近,但前者叶常为倒卵形,具尾尖,后者叶狭披针形,渐尖。展开更多
基金Supported by General Program of Jinhua City Science and Technology Bureau(2009-2-020)~~
文摘[Objective] To utilize the germplasm resources,new systematic evidence for the classification of sect.Paracamellia,genus Camellia is found in this article.[Method] Fruit anatomy of 10 species representing sect.Paracamellia was investigated.And the main component of sect.Paracamellia in the genus Camellia was studied by MVSP software.[Result] The exocarp was thin;mesocarp was broad,having a large number of stone cells;endocarp was lignified,and separated easily from seed.The principle components analysis suggested that the distance between species was far,showing far relation;the current study did not support the formation of Camellia grijsii（C.grijsii）and Camellia yuhsienensis（C.yuhsienensis）,Camellia shensiensis（C.shensiensis）should not be recognized as a variety of C.yuhsienensis.In addition,it also proved Ming＇s combining of Camellia confusa（C.confusa）and Camellia kissi（C.kissi）was unnatural.[Conclusion] The study provided theoretical basis for the development and application of sect.Paracamellia in the genus Camellia.
基金supported by the National Natural Science Foundation of China(Grant Nos.32202542 and U20A2045)the Project of Major Science and Technology in Anhui Province(Grant No.202003a06020021)+2 种基金the Project of Science and Technology of Yunnan Province(Grant No.202102AE090038)Anhui Provincial Natural Science Foundation(Grant No.2108085QC121)the Natural Science Projects for Colleges and Universities in the Anhui Province(Grant No.KJ2021A0145)。
文摘Osmotic stress caused by low-temperature,drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield,particularly in tea plant.Jasmonic acid(JA)is an important phytohormone involving in plant stress.However,underlying molecular mechanisms of JA modulated osmotic stress response remains unclear.In this study,high concentration of mannitol induced JA accumulation and increase of peroxidase activity in tea plant.Integrated transcriptome mined a JA signaling master,MYC2 transcription factor is shown as a hub regulator that induced by mannitol,expression of which positively correlated with JA biosynthetic genes(LOX and AOS)and peroxidase genes(PER).CsMYC2 was determined as a nuclei-localized transcription activator,furthermore,ProteinDNA interaction analysis indicated that CsMYC2 was positive regulator that activated the transcription of CsLOX7,CsAOS2,CsPER1 and CsPER3via bound with their promoters,respectively.Suppression of CsMYC2 expression resulted in a reduced JA content and peroxidase activity and osmotic stress tolerance of tea plant.Overexpression of CsMYC2 in Arabidopsis improved JA content,peroxidase activity and plants tolerance against mannitol stress.Together,we proposed a positive feedback loop mediated by CsMYC2,CsLOX7 and CsAOS2 which constituted to increase the tolerance of osmotic stress through fine-tuning the accumulation of JA levels and increase of POD activity in tea plant.
基金Support by the National Science Foundation of Sri Lanka(Grant NSF/Felow/01/2011).
文摘Objective:To investigate the antifungal potential of different grades of Sri Lankan orthodox black tea [orange pekoe, broken orange pekoe fannings (BOPF) and Dust No. 1] belonging to the three agro-climatic elevations (low, mid and high). Methods: Antifungal activity was assessedin vitro using methanolic extracts (300μg/disc) and agar disc diffusion bioassay technique against threeCandida species,Candida albicans (C. albicans),Candida glabrata(C. glabrata), andCandida tropicalis. ketoconazole and itraconazole mixture was used as positive control (10μg/disc) and methanol was used as the negative control. The minimum inhibitory concentrations were also determined using standard protocols. Results: None of the extracts were effective againstCandida tropicalis. Furthermore, orange pekoe grade tea belonging to all agro-climatic elevations did not induce any antifungal activity againstC. albicans andC. glabrata as well. Conversely, Dust No. 1 belonging to all three agro-climatic elevations and low-grownBOPF showed moderate antifungal activity againstC. albicans andC. glabrata. Interestingly, the severity of the antifungal effect varied with agro-climatic elevations. The minimum inhibitory concentrations ranged from 64.00–128.00μg/mL againstC. glabrata and 128.00-256.00μg/mL againstC. albicans. Conclusions:Sri Lankan Dust No. 1 andBOPF have marked antifungal activityin vitro and offer promise to be used as a supplementary beverage in prophylaxis and during drug treatment in candidiasis.
文摘描述了山茶属短柱茶组新种Camellia osmantha Ye CX,Ma JL et Ye H.新种为灌木,花白色,微有香气。其苞被不分化为苞片和萼片,多数,10～12枚,花瓣6～8枚,均易脱落;雄蕊长短不一,大部分分离;花柱短,基部连生,先端分离,与短柱茶组特征一致。新种花有香气与窄叶短柱茶C.fluviantlis Hand.-Mzt.接近,但前者叶常为倒卵形,具尾尖,后者叶狭披针形,渐尖。
