Analysis on Grain Traits and Sedimentation Value of Wheat Genotype Heng 9966, Its Parents and Their Closely Related Varieties

[Objective] 7 indictors of wheat genotype Heng 9966, its parents and their closely related varieties were analyzed based on the 1000-grain weight, sedimentation value and traits of selected grains in DUS test, so as to provide references for the protection of variety intellectual property. [Method] Testing comparison and statistical analysis were conducted to the dormant seeds of winter wheat genotype 9966, parents of Liangxing 99 and Liangxing 96 aw well as the closely related variety of the parents, Jimai 22. [Result] Heng 9966 showed a difference of 2 codes in 1000-grain weight and sedimentation value from the above-mentioned 3 genotypes, and the variant analysis reached the extremely significant level of 0.01. And it also showed extremely significant difference at the level of 0.01 with Liangxing 66 in grain length, with Liangxing 66 and Jimai 22 in grain width, with Liangxing 66 in grain area, with Liangxing 66 and Jimai 22 in grain perimeter. [Conclusion] Heng 9966 had significant distinctness from its parents and their closely related variety, and grain shape can be used as a reference indicator for variety distinctness for the protection of variety intellectual property.

Detection of QTLs with Additive Effects, Epistatic Effects, and QTL × Environment Interactions for Zeleny Sedimentation Value Using a Doubled Haploid Population in Cultivated Wheat

In order to understand the genetic basis for Zeleny sedimentation value （ZSV） of wheat, a doubled haploid （DH） population Huapei 3 × Yumai 57 （Yumai 57 is superior to Huapei 3 for ZSV）, and a linkage map consisting of 323 marker loci were used to search QTLs for ZSV. This program was based on mixed linear models and allowed simultaneous mapping of additive effect QTLs, epistatic QTLs, and QTL x environment interactions （QEs）. The DH population and the parents were evaluated for ZSV in three field trials. Mapping analysis produced a total of 8 QTLs and 2 QEs for ZSV with a single QTL explaining 0.64-14.39% of phenotypic variations. Four additive QTLs, 4 pairs of epistatic QTLs, and two QEs collectively explained 46.11% of the phenotypic variation （PVE）. This study provided a precise location of ZSV gene within the Xwmc 93 and GluD1 interval, which was designated as Qzsv-1D. The information obtained in this study should be useful for manipulating the QTLs for ZSV by marker assisted selection （MAS） in wheat breeding programs.

Composition and Content of High-Molecular-Weight Glutenin Subunits and Their Effects on Wheat Quality

Sedimentation values, flour glutenin macropolymer (GMP) contents, composition and contents of high-molecular-weight (HMW) glutenin subunits (GS) of 233 flour samples were determined. Our data indicated that subunit 1 occurred more frequently at Glu-A1 , subunit pair 7 + 8 at Glu-B1 and 2 + 12 at Glu-D1. The significant relationships between Glu-1 quality score and total HMW glutenin content, sedimentation value and GMP content suggested that the composition of HMW-GS affects wheat quality strongly. Moreover, the total content of HMW-GS was correlated with certain quality parameters more significantly. Relationship between subunit 5 + 10 content and breadmaking quality was better than others, but 2 + 12, 7 + 8, 7 + 9 and 4 + 12 also correlated with certain quality parameters significantly. The contents of total HMW-glutenin, x-type subunits and y-type subunits related with sedimentation value, flour GMP content, and Glu-1 quality score more strongly than that of individual subunit or subunit pair. The flour GMP content, with excellent correlation to sedimentation value, total contents of HMW glutenin, x- and y-type subunits and many other quality parameters, could be an ideal indicator of breadmaking quality at earlier generations for breeding purpose for its simple procedure and small scale.